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负载骨形态发生蛋白2水凝胶诱导牙髓干细胞的成骨分化

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中国组织工程研究 2025年第16期29卷 3301-3310页

作者：伊斯拉尔古丽·麦麦提贾森刘佳新疆医科大学第一附属医院(附属口腔医院)儿童口腔科-口腔预防科新疆维吾尔自治区乌鲁木齐市830054 西安医学院口腔医学院陕西省西安市710021 新疆维吾尔自治区口腔医学研究所新疆维吾尔自治区乌鲁木齐市830054

背景:前期研究证明,甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶支架可促进人牙髓干细胞的增殖及分化。水凝胶负载骨形态发生蛋白2被认为是骨修复中有前景的材料。目的:观察负载不同质量浓度骨形态发生蛋白2的甲基丙烯酸酐改性明... 详细信息

背景:前期研究证明,甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶支架可促进人牙髓干细胞的增殖及分化。水凝胶负载骨形态发生蛋白2被认为是骨修复中有前景的材料。目的:观察负载不同质量浓度骨形态发生蛋白2的甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶对人牙髓干细胞成骨向分化的诱导作用。方法:制备含0,50,100,200μg/mL骨形态发生蛋白2的甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶,分别记为GelMA/TDM、BMP-2(50 ng/mL)GelMA/TDM、BMP-2(100 ng/mL)GelMA/TDM、BMP-2(200 ng/mL)GelMA/TDM,检测复合水凝胶对骨形态发生蛋白2的体外缓释性能。采用改良组织块酶消化法提取人牙髓干细胞,分别接种于4种水凝胶表面,采用CCK-8法检测细胞增殖,DAPI染色检测细胞黏附;对各组水凝胶表面的人牙髓干细胞进行成骨诱导,进行碱性磷酸酶染色、碱性磷酸酶活性检测与茜素红染色,采用RT-PCR法检测相关成骨基因(Runx2、骨形态发生蛋白2、骨桥蛋白、骨钙素、Ⅰ型胶原)表达。结果与结论:①甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶可持续释放骨形态发生蛋白2长达21 d,在第3-6天释放较快,第6天之后释放趋于平稳;②4种水凝胶均可促进人牙髓干细胞的增殖,其中以BMP-2(100 ng/mL)-GelMA/TDM复合水凝胶的作用最明显;相较于GelMA/TDM复合水凝胶,BMP-2-GelMA/TDM复合水凝胶可促进人牙髓干细胞的黏附,其中以BMP-2(200 ng/mL)-GelMA/TDM复合水凝胶的作用最明显;③相较于GelMA/TDM复合水凝胶,BMP-2-GelMA/TDM复合水凝胶可提升碱性磷酸酶活性、钙结节含量与相关成骨基因表达,综合分析显示BMP-2(100 ng/mL)-GelMA/TDM复合水凝胶的作用更明显;④结果表明,BMP-2(100 ng/mL)-GelMA/TDM复合水凝胶促进牙髓干细胞成骨向分化的能力更明显。

关键词：人牙髓干细胞骨形态形成蛋白2 细胞增殖细胞分化组织工程牙髓-牙本质再生

New Types of Exact Solutions for （N＋1）-Dimensional φ^4 Model

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Communications in Theoretical Physics 2006年第1X期46卷 91-96页

作者： JIA Man LOU sen-Yue Center of Nonlinear Science and Department of Physics Ningbo University Ningbo 315211 China Department of Physics Shanghai Jiao Tong University Shanghai 200030 China

New types of exact solutions of the （N ＋ 1）-dimensional φ^4-model are studied in detail. Some types of interaction solutions such as the periodic-periodic interaction waves and the periodic-solitary wave interacti... 详细信息

关键词： φ^4-model periodic-periodic interaction waves

Integrating value-chain approach with participatory multicriteria analysis for sustainable planning of a niche crop in Indian Himalayas

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Journal of Mountain Science 2019年第10期16卷 2417-2434页

作者： Sudeshna Maya sen Arun KANSAL Coca-Cola Department of Regional Water Studies TERI School of Advanced Studies

The value-chain approach(VCA)was used for exploring how a niche crop,namely large cardamom(Amomum subulatum Roxb.),can be developed sustainably to increase incomes and enhance rural livelihoods in the *** cardamom is a high-value,agro-climatically suitable,and non-perishable spice crop grown in the Himalayan *** in Sikkim,the crop is important to the local economy and is sold in both domestic and international markets;however,its production in India has been declining significantly in recent *** VCA and participatory methods of data collection the study helped elicit value chain actors’perspectives on various reasons for the decline as well as potential strategies to improve the cardamom value *** results present the multiple environmental and climatic(*** stress),social(*** participation)and institutional(*** of trust between actors)challenges and corresponding upgrading *** participatory multi-criteria analysis(MCA),the strategies were prioritized based on the overall preferences of multiple *** analysis considered eight criteria and 25 options,or strategies,and found that that marketability and profitability were the most important criteria,and increasing cardamom production,the most important *** discussions along with sensitivity analysis also showed how these options could be implemented and revealed how criteria preferences can change with expert *** combination of VCA and MCA,because it supports participatory decision-making and balances multiple dimensions of sustainable development,can be effectively deployed in planning for agricultural development in the mountains.

关键词： Large Cardamom Participatory decision-making Upgrading strategies Climate change adaptation Sikkim Sustainable Value Chain

Strongly Approximative Similarity of Operators

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Acta Mathematica Sinica,English Series 2009年第6期25卷 923-930页

作者： You Qing JI sen ZHU Department of Mathematics Jilin University Changchun 130012 P. R. China Institute of Mathematics Jilin University Changchun 130012 P. R. China

Two operators A, B ∈ B（H） are said to be strongly approximatively similar, denoted by A -sas B, if （i） given ε 〉 0, there exist Ki ∈ B（H） compact with ｜｜Ki｜｜〈ε（i = 1,2） such that A＋K1 and B ＋ K2 are similar; （ii） σ0（A） = σ0（B） and dim H（λ; A） = dim H（λ; B） for each λ ∈ σ0（A）. In this paper, we prove the following result. Let S,T ∈ B（H） be quasitriangular satisfying：（i） σ（T） = σ（S） = σw（S） is connected and σe（S） = σlre（S）; （ii） ρs-F（S） ∩ σ（S） consists of at most finite components and each component Ω satisfies that Ω = int Ω, where int Ω is the interior of Ω. Then, S -sas T if and only if S and T are essentially similar.

关键词： compact operator quasitriangular operator Cowen-Douglas operator essentially similar

Coupled Nonlinear Schrodinger Equation: Symmetries and Exact Solutions

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Communications in Theoretical Physics 2009年第1期51卷 27-34页

作者： LIU Ping LOU sen-Yue Department of Physics Shanghai Jiao Tong University Shanghai 200240 China Department of Electronic Engineering University of Electronic Science and Technology of China Zhongshan Institute Zhongshan 528402 China Department of Physics Ningbo University Ningbo 315211 China

The symmetries, symmetry reductions, and exact solutions of a coupled nonlinear Schrodinger （CNLS） equation derived from the governing system for atmospheric gravity waves are researched by means of classical Lie group approach in this paper. Calculation shows the CNLS equation is invariant under some Galilean transformations, scaling transformations, phase shifts, and space-time translations. Some ordinary differential equations are derived from the CNLS equation. Several exact solutions including envelope cnoidal waves, solitary waves and trigonometric function solutions for the CNLS equation are also obtained by making use of symmetries.

关键词： coupled nonlinear SchrSdinger equation classical Lie group approach symmetry exact solution

Weakly Strongly Singular Integral Operators on Anisotropic Hardy Spaces and Their Dual Operators

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Acta Mathematica Sinica,English Series 2009年第11期25卷 1849-1864页

作者： Yong DING sen Hua LAN School of Mathematical Sciences Laboratory of Mathematics and Complex Systems (BNU) Ministry of Education China Beijing Normal University Beijing 100875 P. R. China Department of Mathematics Lishui University Lishui 323000 P. R. China

Let A be an expansive dilation. We define weakly strongly singular integral kernels and study the action of the operators induced by these kernels on anisotropic Hardy spaces associated with A.

关键词： anisotropic Hardy space weakly strongly singular operator atom molecule

Modified Two-Scale Model for Low-Grazing-Angle Scattering from Two-Dimensional Sea Surface

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Chinese Physics Letters 2002年第5期19卷 666-669页

作者： Zhang Yan-Dong Wu Zhen-sen School of Sciences Xidian University Xi'an 710071

A hybrid model for describing a fully developed two-dimensional sea surface is presented, which incorporates the sea spectrum into a fractal model, and an improved two-scale model is applied to calculate the scattering coefficient. In this model the slope distribution of the large-scale surface is obtained with a numerical method instead of being assumed, and the projecting and ray tracing methods are applied to obtain the shadow function for arbitrary incident and scattering angles. Moreover the effect of curvature of the large-scale undulating surface on the scattered wave is taken into account. Hence the precision of the results is greatly increased at low-grazing angles. Finally the amplitude and fractal characteristics of the scattered wave are analysed, which are of great significance for distinguishing and detecting targets on the sea.

关键词： Numerical methods

Energy Equality and Uniqueness of Weak Solutions to MHD Equations in L~∞(O,T;L^n(Ω))

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Acta Mathematica Sinica,English Series 2009年第5期25卷 803-814页

作者： Yan YONG Quan sen JIU Institute of Applied Mathematics Academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing 100190 P. R. China School of Mathematical Sciences Capital Normal University Beijing 100037 P. R. China

In this paper, we study the energy equality and the uniqueness of weak solutions to the MHD equations in the critical space L∞（0, T; L^n（Ω）. We prove that if the velocity u belongs to the critical space L∞（0, T; L^n（Ω）, the energy equality holds. On the basis of the energy equality, we further prove that the weak solution to the MHD equations is unique.

关键词： MHD equations weak solutions energy equality uniqueness

Electroless plating of Ni-Zn(Fe)-P alloy on carbon steel sheets

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中国有色金属学会会刊：英文版 2004年第Z1期14卷 157-161页

作者： WANG sen-lin WU Hui-huang College of Materials Science and Engineering Huaqiao University Quanzhou 362021 China State Key Laboratory for Physical Chemistry of Solid Surface Department of ChemistryXiamen University Xiamen 361005 China State Key Laboratory for Physical Chemistry of Solid Surface Department of ChemistryXiamen University Xiamen 361005 China

The autocatalytic deposition of Ni-Zn(Fe)-P alloys were carried out on carbon steel sheets from bath containing nickel sulfate, zinc sulfate, sodium hypophosphite, sodium citrate and boric acid. The effects of pH and mole ratio of NiSO4/ZnSO4 on the deposition rate and the composition of deposit were studied. It is found that the presence of zinc sulfate in the bath has an inhibitory effect on the alloy deposition. As a consequence, the mole fraction of zinc in the deposits never reaches high value, which is less than 18.0%. The structure and surface morphology of Ni-Zn(Fe)-P coatings were charactered by XRD and SEM. The alloys plated at all conditions consist of amorphous phase coexisting with a crystalline cubic Ni phase. The surface morphology of coating is dependent on the deposition parameters.

关键词： electroless plating nickel zinc