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Plant metabolism paves the way for breeding crops with high nutritional value and stable yield

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Science China(Life Sciences) 2021年第12期64卷 2202-2205页

作者： Cheng Jin Chuanying Fang Yuanyuan Zhang alisdair.r.fer.ie Jie Luo College of Tropical Crops Hainan UniversityHaikou 572208China Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476Germany Centre of Plant Systems Biology and Biotechnology Plovdiv 4000Bulgaria

Plant metabolites often harbor diverse bioactivities;for humans,plant-derived bioactive metabolites are indispensable for ***,plants produce these metabolites to keep themselves *** the domestication and improvement of crops,humans largely focused on increased yield to support population *** example,large seed sizes,high seed weights,and low seed shattering were selected in cereal crops,whereas fruit sizes and shapes were selected in vegetable crops.

关键词： crops breeding Plant

The utility of metabolomics as a tool to inform maize biology

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Plant Communications 2021年第4期2卷 66-88页

作者： David B.Medeiros Yariv Brotman alisdair.r.fer.ie Max Planck Institute of Molecular Plant Physiology PotsdamGermany Department of Life Sciences Ben-Gurion University of the NegevBeershevaIsrael

With the rise of high-throughput omics tools and the importance of maize and its products as food and bioethanol,maize metabolism has been extensively *** maize is still rich in genetic and phenotypic variation,yielding a wide range of structurally and functionally *** maize metabolome is also incredibly dynamic in terms of topology and subcellular *** this review,we examine a broad range of studies that cover recent developments in maize *** attention is given to current methodologies and to the use of metabolomics as a tool to define biosynthetic pathways and address biological *** also touch upon the use of metabolomics to understand maize natural variation and evolution,with a special focus on research that has used metabolite-based genome-wide association studies(mGWASs).

关键词： GWAS metabolism metabolite profiling primary metabolites secondary metabolites Zea mays

The conditional mitochondrial protein complexome in the Arabidopsis thaliana root and shoot

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Plant Communications 2023年第5期4卷 172-190页

作者： Youjun Zhang Silvia Martinez Jaime Mustafa Bulut Alexander Graf alisdair.r.fer.ie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut f€ur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany

Protein complexes are important for almost all biological ***,to fully understand how cells work,it is also necessary to characterize protein complexes and their dynamics in response to various cellular ***,the dynamics of protein interaction play crucial roles in regulating the(dis)association of protein complexes and,in turn,regulating biological processes such as ***,mitochondrial protein complexes were investigated by blue native PAGE and size-exclusion chromatography under conditions of oxidative stress in order to monitor their dynamic(dis)*** of enzyme interactions and changes in protein complex abundance were observed in response to oxidative stress induced by menadione *** included changes in enzymatic protein complexes involving g-amino butyric acid transaminase(GABA-T),D-ornithine aminotransferase(D-OAT),or proline dehydrogenase 1(POX1)that are expected to affect proline *** treatment also affected interactions between several enzymes of the tricarboxylic acid(TCA)cycle and the abundance of complexes of the oxidative phosphorylation *** addition,we compared the mitochondrial complexes of roots and *** differences between the two tissues were observed in the mitochondrial import/export apparatus,the formation of super-complexes in the oxidative phosphorylation pathway,and specific interactions between enzymes of the TCA cycle that we postulate may be related to the metabolic/energetic requirements of roots and shoots.

关键词： mitochondrial complexes BN-PAGE size-exclusion chromatography TCA cycle

Plant cell cultures as heterologous bio-factories for secondary metabolite production

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Plant Communications 2021年第5期2卷 23-34页

作者： Tong Wu Sandra M.Kerbler alisdair.r.fer.ie Youjun Zhang Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 114476 Potsdam-GolmGermany Leibniz-Institute für Gemüse-und Zierpflanzenbau Theodor-Echtermeyer-Weg 114979 GrobbeerenGermany Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria

Synthetic biology has been developing rapidly in the last decade and is attracting increasing attention from many plant *** production of high-value plant-specific secondary metabolites is,however,limited mostly to *** is potentially problematic because of incorrect post-translational modification of proteins and differences in protein micro-compartmentalization,substrate availability,chaperone availability,product toxicity,and cytochrome p450 reductase *** other heterologous systems,plant cells may be a promising alternative for the production of high-value *** commercial plant suspension cell cultures from different plant species have been used successfully to produce valuable metabolites in a safe,low cost,and environmentally friendly ***,few metabolites are currently being biosynthesized using plant platforms,with the exception of the natural pigment *** Arabidopsis thaliana and Nicotiana tabacum cell cultures can be developed by multiple gene transformations and CrISPr-Cas9 genome *** that the introduction of heterologous biosynthetic pathways into Arabidopsis and *** is not widely used,the biosynthesis of foreign metabolites is currently limited;however,therein lies great ***,we discuss the exemplary use of plant cell cultures and prospects for using *** and *** cell cultures to produce valuable plant-specific metabolites.

关键词： plant cell culture synthetic biology secondary metabolites

Cucumber malate decarboxylase,CsNADP-ME2,functions in the balance of carbon and amino acid metabolism in fruit

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Horticulture research 2023年第12期10卷 70-83页

作者： Nan Shan Youjun Zhang Yicong Guo Wenna Zhang Jing Nie alisdair.r.fer.ie Xiaolei Sui Beijing Key Laboratory of Growth and Developmental regulation for Protected Vegetable Crops College of HorticultureChina Agricultural UniversityBeijing 100193China Jiangxi Province Key Laboratory of root and Tuber Crops Biology(Jiangxi Agricultural University) Nanchang 330045China Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476 Germany

Central metabolism produces carbohydrates and amino acids that are tightly correlated to plant growth and thereby crop *** is reported to link mitochondrial respiratory metabolism with cytosolic biosynthetic *** the function of malate metabolism-related enzymes in providing carbon has been characterized in some plants,evidence for this role in the fleshy fruit of cucumber is ***,radiolabeled bicarbonate fed into the xylem stream from the cucumber roots was incorporated into amino acids,soluble sugars,and organic acids in the exocarp and vasculature of *** activities of decarboxylases,especially decarboxylation from NADP-dependent malic enzyme(NADP-ME),were higher in cucumber fruit than in the leaf *** localization revealed that CsNADP-ME2 was mainly located in the exocarp and vascular bundle system of *** and gas-exchange analysis indicated that overexpression of CsNADP-ME2 could promote carbon flux into soluble sugars and starch in *** studies combined with metabolic profiling revealed that the downregulation of CsNADP-ME2 in rNA interference(rNAi)lines caused the accumulation of its substrate,malate,in the *** addition to inhibition of glycolysis-related gene expression and reduction of the activities of the corresponding enzymes,increased amino acid synthesis and decreased sugar abundance were also observed in these *** opposite effect was found in CsNADP-ME2-overexpressing lines,suggesting that there may be a continuous bottom-up feedback regulation of glycolysis in cucumber ***,our studies indicate that CsNADP-ME2 may play potential roles in both central carbon reactions and amino acid metabolism in cucumber fruits.

关键词： sugar carbon thereby

Exploring the genic resources underlying metabolites throughmGWASandmQTL inwheat: From large-scale gene identification and pathway elucidation to crop improvement

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Plant Communications 2021年第4期2卷 127-139页

作者： Jie Chen Mingyun Xue Hongbo Liu alisdair.r.fer.ie Wei Chen National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Max-Planck-Institute of Molecular Plant Physiology Potsdam-Golm 14476Germany

Common wheat(Triticum aestivum L.)is a leading cereal crop,but has lagged behind with respect to the interpretation of the molecular mechanisms of phenotypes compared with other major cereal crops such as rice and *** recently available genome sequence of wheat affords the pre-requisite information for efficiently exploiting the potential molecular resources for decoding the genetic architecture of complex traits and identifying valuable breeding ***,the successful application of metabolomics as an emergent large-scale profiling methodology in several species has demonstrated this approach to be accessible for reaching the above *** such productive avenue is combining metabolomics approaches with genetic ***,this trial is not as widespread as that for sequencing technologies,especially when the acquisition,understanding,and application of metabolic approaches in wheat populations remain more difficult and even arguably *** this review,we briefly introduce the techniques used in the acquisition of metabolomics data and their utility in large-scale identification of functional candidate *** progress has been made in delivering improved varieties,suggesting that the inclusion of information concerning these metabolites and genes and metabolic pathways enables a more explicit understanding of phenotypic traits and,as such,this procedure could serve as an-omics-informed roadmap for executing similar improvement strategies in wheat and other species.

关键词： wheat metabolomics mGWAS mQTL pathway elucidation

The metabolic changes that effect fruit quality during tomato fruit ripening

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Molecular Horticulture 2022年第1期2卷 11-29页

作者： Feng Zhu Weiwei Wen Yunjiang Cheng alisdair.r.fer.ie National r&D Center for Citrus Preservation Key Laboratory of Horticultural Plant BiologyMinistry of EducationHuazhong Agricultural UniversityWuhan 430070China Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 114476 PotsdamGolmGermany

As the most valuable organ of tomato plants,fruit has attracted considerable attention which most focus on its quality formation during the ripening process.A considerable amount of research has reported that fruit quality is affected by metabolic shifts which are under the coordinated regulation of both structural genes and transcriptional *** recent years,with the development of the next generation sequencing,molecular and genetic analysis methods,lots of genes which are involved in the chlorophyll,carotenoid,cell wall,central and secondary metabolism have been identified and confirmed to regulate pigment contents,fruit softening and other aspects of fruit flavor ***,both research concerning the dissection of fruit quality related metabolic changes,the transcriptional and post-translational regulation of these metabolic pathways are ***,a weighted gene correlation network analysis of representative genes of fruit quality has been carried out and the potential of the combined application of the gene correlation network analysis,fine-mapping strategies and next generation sequencing to identify novel candidate genes determinants of fruit quality is discussed.

关键词： Tomato ripening Metabolites regulation Fruit quality

CsbZIP1-CsMYB12 mediates the production of bitter-tasting flavonols in tea plants (Camellia sinensis) through a coordinated activator–repressor network

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Horticulture research 2021年第1期8卷 1495-1512页

作者： Xuecheng Zhao Xiangsheng Zeng Ning Lin Shuwei Yu alisdair rfernie Jian Zhao State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University230036 HefeiChina College of Agronomy Anhui Agricultural University230036 HefeiChina Max-Planck-Institute of Molecular Plant Physiology Am Mühlenberg 114476 Potsdam-GolmGermany

Under high light conditions or UV radiation,tea plant leaves produce more flavonols,which contribute to the bitter taste of tea;however,neither the flavonol biosynthesis pathways nor the regulation of their production are well ***,tea leaf flavonols are enhanced by UV-B but reduced by shading ***,CsUGT78A14,CsMYB12,and CsbZIP1 were upregulated by UV-B radiation and downregulated by ***12 and CsbZIP1 bound to the promoters of CsFLS and CsUGT78A14,respectively,and activated their expression ***1 positively regulated CsMYB12 and interacted with CsMYB12,which specifically activated flavonol ***,CsPIF3 and two MYB repressor genes,CsMYB4 and CsMYB7,displayed expression patterns opposite to that of ***4 and CsMYB7 bound to CsFLS and CsUGT78A14 and repressed their CsMYB12-activated *** CsbZIP1 and CsMYB12 regulated neither CsMYB4 nor CsMYB7,CsMYB12 interacted with CsbZIP1,CsMYB4,and CsMYB7,but CsbZIP1 did not physically interact with CsMYB4 or ***,CsPIF3 bound to and activated CsMYB7 under shading to repress flavonol *** combined results suggest that UV activation and shading repression of flavonol biosynthesis in tea leaves are coordinated through a complex network involving CsbZIP1 and CsPIF3 as positive MYB activators and negative MYB repressors,*** study thus provides insight into the regulatory mechanism underlying the production of bitter-tasting flavonols in tea plants.

关键词： coordinated media opposite

Analysis of metabolic alterations in Arabidopsis following changes in the carbon dioxide and oxygen partial pressures

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Journal of Integrative Plant Biology 2014年第9期56卷 941-959页

作者： Alexandra Florian Stefan Timm Zoran Nikoloski Takayuki Tohge Hermann Bauwe Wagner L.Araujo alisdair.r.fer.ie Max Planck Institute for Molecular Plant Physiology Department of Plant Physiology University of Rostock Max-Planck Partner group at the Departament of Plant Biology Federal University of Vicosa

As sessile organisms,plants are subject to a multitude of environmental variations including several which directly affect their interaction with the *** the indiscriminant nature of rubisco,the relative rates of photosynthesis and photorespiration are known to be responsive to changes in gas ***,comprehensive profiling methods have not yet been applied in order to characterize the wider consequences of these changes on primary metabolism in ***,although transcriptional profiling has revealed that a subset of photorespiratory enzymes are co-expressed,whether transcriptional responses play a role in short-term responses to atmospheric compositional changes remains *** address these questions,plants Arabidopsis thaliana（Arabidopsis） ecotype Columbia（Col-O） grown under normal air conditions were transferred to different CO_2 and O_2 concentrations and characterized at the physiological,molecular,and metabolic levels following this *** results reveal alterations in the components,which are directly involved in,or supporting,photorespiration,including transcripts and metabolite *** results further highlight that the majority of the regulation of these pathways is not mediated at the level of transcription and that the photorespiratory pathway is essential also in conditions in which flux through the pathway is minimized,yet suggest that flux through this pathway is not mediated at the level of transcription.

关键词： Carbon dioxide partial pressures gene expression metabolic profiling oxygen partial pressures