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Antitumor Effect of Cationic INKKI Peptide from Bovine <i>β</i>-Casein on Melanoma B16F10

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Journal of Cancer Therapy 2012年第4期3卷 237-244页

作者： Ricardo Alexandre Azevedo Adilson Kleber ferreira aline Vivian Vatti Auada Kerly Fernanda Mesquita Pasqualoto Rafael Marques-Porto Durvanei Augusto Maria Ivo Lebrun Butantan Institute Biochemistry and Biophysical Laboratory Sao Paulo Brazil

Cationic peptide with the sequence INKKI 41-45 was isolated from bovine β-casein after tryptic hydrolysis and synthetized. The aim of this work was to evaluate the antiproliferative activity in vitro and antitumor effect in animal model. The in vitro cytotoxicity was evaluated on B16F10 melanoma cells by MTT assay. Detection of apoptosis was measured using the annexin V/PI double staining and cell cycle analysis performed flow cytometry. Caspase-3 activity was analyzed with substrate specific fluorogenic DEVD-MCA. In vivo, antitumor activity was evaluated in B16F10 melanoma tumor-bearing C57BL/6J mice. The animals were treated with 55 mg/kg INKKI administered into peritumoral region, while control group received saline solution. The following antitumor parameters were examined: tumor volume, number of metastases, tumor delayed time, tumor doubling time. Histological analyses were performed with H & E staining. The results showed that INKKI induced dose-response cytotoxicity selective for B16F10 melanoma cells (IC50 1.7 μM) and did not present cytotoxic effects for FN1 fibroblast cells. INKKI-induced apoptosis detected trough of annexin V/PI assay and it was accompanied with an increase of sub-G1 apoptotic fractions and significant increase of caspase-3 cleavage. The tumor-bearing mice treated with INKKI showed a significant reduction in tumor volume of 72.62% and decreased of metastasis number loci. In addition, INKKI caused a significant delay in tumor growth and prolonged the tumor doubling time. Histological analysis revealed an increased of necrosis areas and reduction of tumor cells in tumor treated with INKKI, it was a many hallmark of its antitumor effects observed from in vivo experiments. In conclusion, we show that INKKI is a peptide that could be considered a new putative candidate development to anticancer therapy drug.

关键词： β-Casein Peptide Apoptosis Metastasis Tumor Growth Melanoma

重视孵化场清洁消毒可获取良好健康和免于人畜共患病(续1)

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国外畜牧学（猪与禽） 2018年第12期38卷 42-44页

作者：蔡霞(译) 刘珅(译) 王晶晶(校) aline Kuntze ferreira 不详安伟捷公司

孵化场想要有理想的生产性能,重视清洗消毒很重要。有效的清洁消毒必须清除表面的有机和无机残留物、外来物以及微生物以防止生物膜的形成。

关键词：孵化场消毒人畜共患病

Interrelationship between chromosome 8 aneuploidy,C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

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World Journal of Gastroenterology 2006年第38期12卷 6207-6211页

作者： Danielle Queiroz Calcagno Mariana ferreira Leal aline Damaceno Seabra Andre Salim Khayat Elizabeth Suchi Chen Samia Demachki Paulo Pimentel Assumpcao Mario Henrique Girao Faria Silvia Helena Barem Rabenhorst Márcia Valéria Pitombeira ferreira Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano Human Cytogenetics and Toxicological Genetics Laboratory Department of BiologyCenter of Biological SciencesFederal University of ParáBelémPABrazil Genetics Division Department of Morphology Federal University of Sao PauloSao PauloSPBrazil Department of Pathology and Surgery Service Federal University of ParáBelémPABrazil Joao de Barros Barreto University Hospital Federal University of ParáBelémPABrazil Molecular Genetics Laboratory Department of Pathology Medical SchoolFederal University of CearáFortalezaCEBrazil

AIM: To investigate chromosome 8 numerical aberra- tions, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histo- pathological characteristics of gastric tumors. METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immu- nostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases showed chromosome 8 aneu- ploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level ofchromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal- type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic path- ways.

关键词： Chromosome 8 aneuploidy C-MYC amplification Immunostaining Gastric adenocarcinoma

Differences in age modulates neutrophils function

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Advances in Biological Chemistry 2014年第1期4卷 51-58页

作者： Claudia J. Lagranha Tatiana C. Alba-Loureiro aline Isabel da Silva Danuta Duarte Bezerra de Lima Anderson Apolonio Pedroza Diorginis ferreira Tania Pithon-Curi Department of Nutrition Federal University of Pernambuco Recife Brazil Department of Physical Education and Sports Science Federal University of Pernambuco Vitoria de Santo Ant&atilde o Brazil Department of Physiology and Biophysics Institute of Biomedical Sciences University of S&atilde o Paulo S&atilde o Paulo Brazil Post-Graduate Program in Human Movement Science Biological Sciences and Health Center Cruzeiro do Sul University Sao Paulo Brazil

The sexual maturation in all mammals is the period in which the quiescent gonads are activated by gonadotropins from anterior pituitary, increasing the secretion of sexual hormones. Sexual maturation it is also related with the development of several other body features such as body mass and maturation of the circulatory, skeletal and hematopoietic systems. The aim of the present study was to evaluate the function of neutrophils submitted to in vivo lower and higher concentration of testosterone (sexually immature: 60 days and sexually mature: 90 days). Using different approaches we evaluated cell viability and function and gene expression in rat neutrophils from 60 and 90 days-old animals. Neutrophils from 90 days-old rats showed a decrease in phagocytic and fungicidal capacity, without change in cellular viability. Additionally, we verified that sexual maturation induced increase in production of reactive oxygen and nitrogen species (RONS) and also in TNF-α, IL-6 and IL-10 cytokines. In conclusion, our data suggest that increase in testosterone levels induced significant alteration in neutrophil function, impairing phagocytic capacity.

关键词： Testosterone Neutrophils Function NADPH-Oxidase Components Sexual Maturation

Hepatocarcinoma with metastasis to the anterior mediastinum

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Hepatoma Research 2016年第1期2卷 293-296页

作者： Rogerio Camargo Pinheiro Alves Lorena Sagrilo Auer Lisa Rodrigues da Cunha Saud Raquel Coris Arrelaro aline Carboni Casado Bruno Gustavo ferreira Denis Szjenfeld Paula Bechara Poletti Department of Hepatology Instituto de Assistencia Medica ao Servidor Publico Estadual04028-000 Sao PauloBrazil Department of Gastroenterology Instituto de Assistencia Medica ao Servidor Publico Estadual04028-000 Sao PauloBrazil Department of Radiology Instituto de Assistencia Medica ao Servidor Publico Estadual04028-000 Sao PauloBrazil

Liver malignancies are the sixth leading cause of cancer worldwide,whereas hepatocellular carcinoma(HCC)is the most frequent histological type of liver *** metastasis,which rarely involves the mediastinum,is associated with poor *** 80-year-old male presenting with mild diffuse abdominal pain for 4 months,associated with hyporexia,increased abdominal volume,dry cough,and loss of 4 kg in 1 month,sought medical assistance due to hemoptysis and chest *** study revealed HCC with mediastinal metastasis,after which sorafenib therapy was *** progressed to death 4 months after the start of the treatment.

关键词： Hepatocellular carcinoma mediastinal metastasis sorafenib computed tomography

重视孵化场清洁消毒可获取良好健康和免于人畜共患病

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国外畜牧学（猪与禽） 2018年第11期38卷 17-19页

作者：蔡霞(译) 耿拓宇(校) 王晶晶(审) 靳文广(制图表) aline kuntze ferreira 不详安伟捷公司

孵化场想要有理想的生产性能,重视清洗消毒很重要.孵化场是生产链中一个共同的环节,单个孵化场每月可生产几百万羽雏鸡,在一体化生产中没有哪儿比孵化场更重要了.此外,由于消费者和市场对肉鸡生产中减少抗生素使用的压力一直持续不断,... 详细信息

孵化场想要有理想的生产性能,重视清洗消毒很重要.孵化场是生产链中一个共同的环节,单个孵化场每月可生产几百万羽雏鸡,在一体化生产中没有哪儿比孵化场更重要了.此外,由于消费者和市场对肉鸡生产中减少抗生素使用的压力一直持续不断,因此从产蛋到出雏实行有效的消毒程序以限制细菌和霉菌的生长已变得必不可少.

关键词：孵化场人畜共患病清洁消毒健康生产性能一体化生产清洗消毒肉鸡生产

Determination of isotretinoin in pharmaceutical formulations by reversed-phase HPLC

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Journal of Biomedical Science and Engineering 2010年第5期3卷 454-458页

作者： Carla Aiolfi Guimaraes Farid Menaa Bouzid Menaa Ivo Lebrun Joyce S. Quenca-Guillen aline Vivian Vatti Auada Lucildes P. Mercuri Paula ferreira Maria Inês Rocha Miritello Santoro 不详

The development of facile and rapid quantification of biologically active biomolecules such as isotretitoin in therapeutic drugs contained in many generic formu- lations is necessary for determining their efficiency and their quality to improve the human health care. Isotretritoin finds its applications in the maintenance of epithelial tissues. Different processes to date such as normal phase HPLC, or gas chromatrography am- ong others are able to separate and quantify isote- troin. However, the extraction is quite complex and in the case of HPLC, the analysis requires long retention times. In such context, an isocratic reversed- phase high-performance liquid chromatography (HP- LC) technique coupled with an UV-vis detector is described here for easy separation and quantification of 13-cis-retinoic (isotretinoin) from soft gelatin capsule formulations. The isotretinoin was extracted from three different commercial drug samples with tetrahydrofuran (THF) solvent by a procedure that can be completed in less than 10 minutes. Subsequent separation and quantification were accomplished in less than 5 minutes under isocratic reversed-phase conditions on a Lichrospher RP18 column and a mobile phase consisting of 0.01% TFA/acetonitrile (15/85, v/v) at a flow rate of 1.0 mL/min. Isotretoin was detected for the three samples via its UV-vis absorbance at 342 nm. The method was validated and the results showed good linearity, precision and accuracy for sensitive and selective quantitative determination of isotretinoin in the different pharmaceutical formulations. We found that the average isotretinoin content in two of the three commercial pro- ducts fell outside the 90-110% United States Pha- rmacopeia specifications. Consequently, the facile extraction and the precise method for the biomole- cule quantification open up tremendous possibilities in improving the quality control of drugs which can exist as different generic brands.

关键词： 13-Cis Retinoic Acid Reversed-Phase Chromatography Isotretitoin Extraction Isotretinoin Quantification Pharmaceuticals Formulation

Economic Viability of Production of Tree Parica （Schizolobium amazonicum Huber ex. Ducke） of Reforestation Project in the Municipality Paragominas-PA, Brazil

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Journal of Life Sciences 2014年第12期8卷 967-971页

作者： Manoel Tavares de Paula Altem Nascimento Pontes Helio Raymundo ferreira Filho Lucy Anne Cardoso Lobao Gutierrez Ismael Matos da Silva Maria da Conceicao Silva Damasceno aline Lima de Sena Center of Natural Science and Technology University of Pare State Belem 66095-100 Para State Brazil Center of Social Sciences and Education University of Pare State Belem 66095-100 Para State Brazil

The objective of this study was to analyze the profitability of production Parica reforestation in the municipality of Paragominas-PA. The study was conducted in the municipality of Paragominas Messoregiao located in the northeast of Para. The basic data used in the economic evaluation of production of planting parica （costs and revenues） were derived from primary source through raising production cost of parica with farmers from the municipality, analyzing the period of production horizon of seven years. The discount rate chosen for economic evaluation was 10% per year. NPV （net present value） calculation IRR （internal rate of return）, BCR （benefit-cost ratio） and EP （break-even point）. To check the economic feasibility of producing parica the following criteria were used for economic analysis. The results for NPV, IRR and BC ratio were respectively of $495,970, 28.83% and 2.43. For EP （break-even point） the value of 41.08% was found, which demonstrates the minimum amount of production, so that revenue equals costs. Based on the results obtained, it was found that the reforestation model with parica proposed in this paper presents economically viable for deployment.

关键词： Amazon parica reforestation

Dimensions of Access to Antihypertensive Medications in Ceilandia,Distrito Federal,Brazil

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Health 2015年第11期7卷 1517-1526页

作者： Fabiana Xavier Cartaxo Salgado Dayani Galato Gislane ferreira de Melo Marileusa Dosolina Chiarello aline Gomes de Oliveira Letícia Farias Gerlack Micheline Marie Milward de Azevedo Meiners Margo Gomes de Oliveira Karnikowski Graduate Program in Health Sciences and Technology Campus of CeilandiaUniversity of Brasília(FCE/UnB)BrasíliaBrazil Graduate Program in Gerontology Catholic University of Brasília(UCB)BrasíliaBrazil Graduate Program in Public Health University of Brasília(UnB)BrasíliaBrazil

Access can be understood as the sum of a number of elements of the interface between patients and the health care system. This study took a comprehensive approach to the dimensions of access to medications, employing indicators to evaluate the dimensions of access to antihypertensive medications in Ceilandia, DF, Brazil. This was a cross-sectional epidemiological study, administering questionnaires during home visits. The survey covered epidemiological and socioeconomic profiles, behavioral habits and the dimensions of access to antihypertensive medications comprising physical, financial, and geographic availability and accept ability according to the hypertensive population of Ceilandia. The total sample comprised 400 individuals and the hypertensive subset numbered 140 (35%). Indicators of physical availability of medications revealed that users found it difficult to acquire their drugs on almost one third of occasions and in some cases were unable to access any of these products. The greatest barriers to access were reported by users of pharmacies belonging to the Brazilian National Health Service (SUS) and on the “People’s Pharmacies” network. More than one third of the hypertensive sample spent their own money on medications they could not find at these pharmacies. The majority of the hypertensive subsets were overweight/obese, a minority engaged in physical activity and 40% were smokers/ex-smokers. More women reduced their salt intake. Men had higher incomes, educational level, and socioeconomic status. Failure to keep the public health care system supplied has prejudiced access to essential medications for hypertension treatment, transferring the costs onto users. This population has lifestyle habits that increase the risk of exacerbation of hypertension. These results reveal a need for effective public policies to ensure access to antihypertensive medications and involve users of the health care system in changing their habits and behaviors in order to achie

关键词： Health Care Access Systemic Arterial Hypertension Drug Treatments