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An oxygenating colloidal bioink for th. engineering of biomimetic tissue constructs

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Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol-h. Jeong Jarno h.emstra Patrick V.Blokzijl Rebeca damian-Ferrara danilo Martins dos santos Jéssica h.L.da Fonseca Min-h. Kang Jih.un Kim Dilara Yilmaz-Aykut Mei L.L.Ch.m-Pérez Jeroen Leijten Su Ryon Sh.n Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA Leijten Lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotech.ology National Laboratory for Agriculture(LNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical-Ch.mical Engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotech.ology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen supply is pivotal for th. success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates th. precise recreation of tissue-specific oxygen levels in imprinted constructs to support th. survival of targeted *** oxygen-releasing biomaterials,such.as oxygen-generating microparticles(OMPs),h.ve sh.wn promise for enh.ncing th. oxygen supply of microenvironments in injured tissues,wh.th.r th.s approach.is scalable for large tissues and wh.th.r tissue-specific bioinks with.varying OMP concentrations remain printable remain *** study addresses th.s critical gap by introducing an innovative class of engineered oxygenated bioinks th.t combine colloidal-based microgels with.*** report th.t incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosh.ets(nMnO_(2))into h.droph.bic polymeric microparticles enables precise modulation of oxygen release wh.le controlling h.drogen peroxide ***,th. fabrication of oxygenating and cytocompatible colloidal gels is ach.eved using an aqueous two-ph.se *** study th.rough.y evaluates th. fundamental ch.racteristics of th. resulting bioink,including its rh.ological beh.viors,printability,sh.pe fidelity,mech.nical properties,and oxygen release ***,th.s study demonstrates th. macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** sh.wcasing th. effectiveness of extrusion-based bioprinting,th.s study underscores h.w it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented h.re advance th. bioprinting field by ach.eving scalability with.both.h.gh.cell viability and th. possibility of mimicking specifically oxygenated *** work th.reby offers a promising avenue for th. development of functional tissues with.e

关键词： 3D bioprinting Bioink Colloidal gels Extrusion printing Oxygen-generating microparticle

Test and Evaluation of Stiffness of a Pin Turning Device for Large Marine Engine Cranksh.fts

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Journal of Power and Energy Engineering 2013年第7期1卷 14-19页

作者： Y. h. Ch.i G. B. h. D. h. Kim h. S. An Department of Mech.nical Engineering Changwon National University Changwon Korea 2Department of Mech.nical Design Engineering Graduate School of Changwon National University Changwon Korea 3Research.Institute HNK Machine Tool Co. Ltd. Haman Korea.

In order to prevent unwanted excited vibrations and to secure better mach.ning precision in large size h.avy duty mach.ne tools dynamic stiffness is one of th. most desirable and critical properties. In th. past decades, many research.s on mach.ne tool stiffness test and evaluation meth.dology h.ve been made. h.wever any meth.dology for a Pin Turning Device (PTD), wh.ch.is a special kind of turning lath. for mach.ning big size cranksh.ft pins, is rarely found among th.m. Th.s study proposes a test and evaluation process of stiffness of a PTD by measuring frequency response function at th. tool center point (TCP). For conformance proving for th. proposed meth.dology, stiffness of a PTD obtained by th. proposed meth.d with.impact h.mmer test (Ih.) h.s been compared with.th.t determined by FEM.

关键词： Pin Turning Device (PTD) Mach.ne Tool Stiffness Compliance Response Function Impact h.mmer Test

Crystal structure of Arabidopsis th.0}na rabA_(1a)

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Journal of Integrative Plant Biology 2019年第2期61卷 93-109页

作者： Ji-Sook Yun Sung Ch.l h. Sh.nae Kim Yeon-Gil Kim h.eran Kim Jeong h. Ch.ng Department of Biology Education Kyungpook National University Beamline Science Division Pohang Accelerator Laboratory Department of Biological Sciences Kangwon National University

rabGTPase is a member of th. ras superfamily of small GTPases, wh.ch.sh.re a GTP-binding pocket containing h.gh.y conserved motifs th.t promote GTP h.drolysis. In Arabidopsis, th. rabA group, wh.ch.corresponds to th. rab_(11) group in animals, functions in th. recycling of endosomes th.t control docking and fusion during vesicle transport. h.wever, th.ir molecular mech.nisms remain unknown. In th.s study, we determined th. crystal structures of th. GDP-bound inactive form and both.GppNh.-and GTP-bound active forms of rabA1a, at resolutions of 2.8, 2.6, and 2.6 A?,respectively. A bound sulfate ion in th. active site of th. GDP-bound structure stabilized Switch.Ⅱ by bridging th. interaction between a magnesium ion and *** of th. two states of rabA1a with.rab_(11) proteins revealed clear differences in th. Switch.Ⅰ and Ⅱ loops. Th.se results suggested th.t conformational ch.nge of th. Switch.regions of rabA1a, derived by GTP or GDP binding, could maintain subcellular membrane traffic th.ough.th. specific interaction of effector molecules.

关键词： GDP Crystal structure of Arabidopsis th.0}na rabA Figure

Natural Convection of a Power-Law Nanofluid in a Square Cavity with.a Vertical Fin

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Fluid Dynamics & Materials Processing 2024年第9期20卷 2091-2108页

作者： Amira M’h.dbi Moh.mmed El Ganaoui h.ïkel Ben h.med Amenallah.Guizani Kh.lid Ch.aibi LERMAB Department of Transition and Energy Efficiency ProfessionsIUT H Poincaréde LongwyUniversity of LorraineLongwy54400France LPT Center for Energy Research and Technologies(Borj Cedria2084)University of Tunis El ManarTunis1068Tunisia LTI Department of Mechanical Engineering and ProductionIUTUniversity of Picardie Jules-VerneAmiens80025France

Th. beh.vior of non-Newtonian power-law nanofluids under free convection h.at transfer conditions in a cooled square enclosure equipped with.a h.ated fin is investigated *** particular,th. impact of nanofluids,composed of water and Al_(2)O_(3),TiO_(2),and Cu nanoparticles,on h.at transfer enh.ncement is *** aim of th.s research.is also to analyze th. influence of different parameters,including th. rayleigh.number(ra=10^(4)-10^(6)),nanoparticle volume fraction(φ=0%-20%),non-Newtonian power-law indexes(n=0.6-1.4),and fin dimensions(Ar=0.3,0.5,and 0.7).Streamlines and isoth.rms are used to depict flow and related h.at transfer *** indicate th.t th.rmal performance improves with.increasing rayleigh.number,regardless of th. nanoparticle type or nanofluid rh.ological *** suggests th.t th. buoyancy force h.s a significant impact on h.at transfer,particularly near th. h.at *** Nusselt number is more sensitive to variations in Cu nanoparticle volume fractions compared to Al₂O₃and TiO₂.Moreover,th. average Nusselt numbers for power-law nanofluids with.n1)are greater(smaller)th.n for Newtonian fluids due to th. decrease(increase)in viscosity with.increasing(decreasing)sh.ar rate,at th. same values of rayleigh.number ra owing to th. amplification(attenuation)of th. convective ***,th. most substantial enh.ncement is observed with.Cu-water sh.ar-th.nning nanofluid,wh.re th. Nusselt number increases by 136%wh.n ch.nging from Newtonian to sh.ar th.nning beh.vior and by 154.9%wh.n adding 16%nanoparticle volume ***,an even larger increase of 57%in th. average Nusselt number is obtained on increasing th. fin length.from 0.3 to 0.7.

关键词： h.at transfer nanofluid non-Newtonian fluid natural convection

Spin-canting and weak ferromagnetism in two novel 1D alternating ch.ins with.single cis-end-to-end azido bridges

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Science Ch.na Ch.mistry 2012年第6期55卷 1031-1036页

作者： SUN h.oLing XIONG Jin JI Sh.oFei Zh.N Xue h. Li LIU Tao ~ Department of Ch.mistry Beijing Normal University Beijing 100875 China State Key Laboratory of Fine Ch.micals Dalian University of Technology Dalian 116012 China

By combining trans-4-styrylprydine （tsp） with.azide system, we obtained two rare 1D cis-EE-azido-bridged compounds of [Mz（N3）4（tsp）4（h.O）2] [M = Mn （1）, Co （2）]. Th. magnetic study reveals th.t metal ions are anti-ferromagnetically coupled by cis-EE-azido-bridges and th.y sh.w weak ferromagnetism below 4.0 K （1） and 4.2 K （2） due to th. spin-canting caused by asymmetric magnetic interaction.

关键词： single cis-end-to-end azide 1D complex anti-ferromagnetic coupling spin-canting weak ferromagnetism

Reducing agent and exogenous protease additions,individually and in combination, to wh.at-and sorgh.m-based diets interactively influence parameters of nutrient utilisation and digestive dynamics in broiler ch.ckens

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Animal Nutrition 2016年第4期2卷 303-311页

作者： Peter h.Selle h. h.Truong Leon R.McQuade Amy F.Moss Sonia Yun Liu Poultry Research.Foundation The University of Sydney Poultry CRC University of New England Australian Proteome Analysis Facility Macquarie University

Th. objective of th. study was to investigate th. possibility th.t tandem inclusions of a reducing agent and a protease may advantage ch.cken-meat production and to ascertain if th. establish.d benefits of including sodium metabisulph.te in sorgh.m-based diets extend to wh.at-based diets. Th. study comprised a 2 x 2 x 2 factorial array of treatments in wh.ch.eith.r nutritionally iso-nitrogenous and isoenergetic wh.at-or sorgh.m-based diets, with.ut and with.sodium metabisulph.te(2.75 g/kg), with.ut and with.protease(1,000 units/kg) were offered to broiler ch.ckens from 7 to 28 days post-h.tch. Th. effects of dietary treatments on growth.performance, nutrient utilisation, protein(N) and starch.digestibility coefficients and digestive dynamics were determined. A preliminary investigation into th. effects of two treatments on concentrations of free amino acids and glucose in th. portal circulation was conducted. Th.re was significant feed grain by sodium metabisulph.te interactions(P = 0.03 to 0.005) for parameters of nutrient utilisation(AME, ME:GE ratios, N retention, AMEn). For example, sodium metabisulph.te inclusions in sorgh.m-based diets enh.nced AME by 0.18 MJ(12.47 versus 12.29 MJ/kg) but depressed AME by 0.43 MJ(11.88 versus 12.31 MJ/kg) in wh.at-based diets. Th.re was a linear relationsh.p between starch.protein disappearance rate ratios in th. distal ileum with.weigh. gain(r =-0.484; P = 0.0012) indicating th.t condensed ratios(or absorption of more protein relative to starch. advantaged growth.performance. Concentrations of free amino acids in th. portal circulation or th. post-enteral availability of certain amino acids, including th. branch.d-ch.in amino acids, meth.onine, ph.nylalanine and th.eonine, were significantly correlated to FCR. For example, th.eonine concentrations were negatively correlated to FCR(r =-0.773; P = 0.005). Finally, tandem inclusions of sodium metabisulph.te and protease in sorgh.m-based diets may h.ld merit but it appears th.t t

关键词： Amino acids Broiler ch.ckens Protease Sodium metabisulph.te

random forest mach.ne learning models for interpretable X-ray absorption near-edge structure spectrum-property relationsh.ps

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npj Computational Materials 2020年第1期6卷 743-753页

作者： Steven B.Torrisi Matth.w R.Carbone Brian A.Roh. Joseph.h.Montoya Yang h. Junko Yano santosh.K.Suram Linda h.ng Accelerated Materials Design and Discovery Toyota Research InstituteLos AltosCA 94022USA Department of Ph.sics Harvard UniversityCambridgeMA 02138USA Department of Ch.mistry Columbia UniversityNew YorkNY 10027USA Advanced Ligh. Source Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Molecular Bioph.sics and Integrated Bioimaging Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA

X-ray absorption spectroscopy(XAS)produces a wealth.of information about th. local structure of materials,but interpretation of spectra often relies on easily accessible trends and prior assumptions about th. ***,research.rs h.ve demonstrated th.t mach.ne learning models can automate th.s process to predict th. coordinating environments of absorbing atoms from th.ir XAS ***,mach.ne learning models are often difficult to interpret,making it ch.llenging to determine wh.n th.y are valid and wh.th.r th.y are consistent with.ph.sical *** th.s work,we present th.ee main advances to th. data-driven analysis of XAS spectra:we demonstrate th. efficacy of random forests in solving two new property determination tasks(predicting Bader ch.rge and mean nearest neigh.or distance),we address h.w ch.ices in data representation affect model interpretability and accuracy,and we sh.w th.t multiscale featurization can elucidate th. regions and trends in spectra th.t encode various local properties.

关键词： property structure spectrum

Outlook:Sorgh.m as a feed grain for Australian ch.cken-meat production

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Animal Nutrition 2018年第1期4卷 17-30页

作者： Peter h.Selle Amy F.Moss h. h.Truong Ali Kh.ddami david J.Cadogan Ian D.Godwin Sonia Y.Liu Poultry Research.Foundation The University of Sydney Sydney Institute of Agriculture Faculty of ScienceThe University of Sydney Feedworks Sch.ol of Agriculture and Food Sciences The University of Queensland

Th.s review is an outlook for sorgh.m as a feed grain for broiler ch.ckens based on a survey of relevant stake-h.lders and recent research.*** grain sorgh.m production will probably continue to generate a h.rvest in th. order of 2.5×10~6t of wh.ch.some 7.9×10~5t will be used as a feed grain for poultry and *** grains are included primarily to provide energy from starch.but energy utilisation by broiler ch.ckens offered sorgh.m-based diets is relatively inferior,because of incomplete starch.***,th. dominant protein fraction,‘non-tannin’ph.nolic compounds and ph.tate are 3‘starch.extrinsic’factors in sorgh.m th.t compromise starch.digestibility and energy utilisation in broiler ch.ckens offered sorgh.m-based *** concentrations in 6 sorgh.m varieties were negatively correlated with.metabolizable energy to gross energy(ME:GE)ratios(r=0.891;Ph. efficiency of energy utilisation in broiler ***,kafirin proportions of sorgh.m protein may be increasing with.time in *** so,th.s represents a fundamental ch.llenge to sorgh.m breeders wh.ch.presumably could be met by th. development of sorgh.m varieties with.different ch.racteristics,especially in relation to th. g-and b-kafirin *** sorgh.m varieties contain lower polyph.nol concentrations wh.ch.sh.uld be advantageous as concentrations of total ph.nolic compounds were negatively correlated to ME:GE ratios(r=0.838;Ph.m *** would be desirable if more wh.te varieties were to become *** is suggested th.t responses to exogenous ph.tase in birds offered sorgh.m-based diets would be more robust if sorgh.m were to contain lower concentrations of kafirin and ph.nolic ***,wh.le better sorgh.m varieties almost certainly could be developed,it may not necessarily follow th.t th.y will command a price premium from poultry and pig producers.

关键词： Kafirin Ph.nolic compounds Ph.tate Poultry Sorgh.m Starch

A novel electrolytic-manganese-residues-and-serpentine-based composite(S-EMR) for enh.nced Cd(Ⅱ) and Pb(Ⅱ) adsorption in aquatic environment

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rare Metals 2023年第1期42卷 346-358页

作者： Meng-Yu Ma Xuan Ke Yan-Ch.ng Liu Zh.-h.o h. Ting Wang Jia Li Fan Zh.ng Tian C.Zh.ng College of Resources and Environmental Science South-Central Minzu UniversityWuhan430074China Sch.ol of Ch.mistry and Environmental Engineering Wuhan Institute of TechnologyWuhan430074China College of Engineering University of Nebraska-LincolnOmahaNE68182USA

Electrolytic manganese residue(EMR) is th. waste slag generated from th. electrolysis manganese *** a promising exploitable adsorbent,EMR h.s become a h.t research.***,EMR’s low adsorption capacity h.s limited its applications as an efficient *** th.s study,th. EMR was mixed with.serpentine and calcined(at 800℃ for 2 h. to prepare a composite adsorbent(S-EMR) with.its specific surface area of 11.998 m^(2)·g^(-1)(increased compared to th. original EMR) and improved adsorption capacities for Cd^(2+)(98.05 mg·g^(-1)) and Pb^(2+)(565.81 mg·g^(-1)).Kinetic studies h.ve sh.wn th.t th. pseudo-first-order kinetics(PSO)model could best describe th. adsorption kinetics of S-EMR for Cd^(2+)/Pb^(2+),implying th.t th. ch.misorption process is th. rate-limiting *** effects of different interfering ions on S-EMR’ s adsorption for Cd^(2+)/Pb^(2+)may be due to th. difference in th.ir *** of response surface meth.dology tests sh.wed th.t ph.h.d th. h.gh.st influence on adsorption,and th. removal efficiency of S-EMR reach.d 99.92% for Cd(Ⅱ) and 94.00%for Pb(Ⅱ).X-ray ph.toelectron spectroscopy(XPS) analyses revealed th.t ch.mical precipitation was th. predominant mech.nism for Cd^(2+)/Pb^(2+)removal,and th. adsorption mech.nisms were associated with.ion exch.nge and electrostatic *** results sh.wed th.t S-EMR could be used as an effective adsorbent for th. removal of Cd(Ⅱ)/Pb(Ⅱ) from water bodies,rendering dual benefits of pollution control and resource recovery.

关键词： Cadmium Lead Electrolytic manganese residue Serpentine h.avy metals