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Complex Coacervate Materials as Artificial Cells

Accounts of Materials Research 2023年第3期4卷 287-298页

作者： alexander b.cook Sebastian Novosedlik Jan C.M.van Hest bio-Organic Chemistry Institute for Complex Molecular SystemsEindhoven University of Technology5600 MB EindhovenThe Netherlands

CONSPECTUS:Cells have evolved to be self-sustaining compartmentalized systems that consist of many thousands of biomolecules and metabolites interacting in complex cycles and reaction *** subtle intricacies of these self-assembled structures are still largely *** importance of liquid−liquid phase separation(both membraneless and mem-brane bound)is,however,recognized as playing an important role in achieving biological function that is controlled in time and *** biochemical reactions in vitro has been a success of the last decades,for example,establishment of the minimal set of enzymes and nutrients able to replicate cellular activities like the in vitro transcription translation of genes to *** than this though,artificial cell research has the aim of combining synthetic materials and nonliving macromolecules into ordered assemblies with the ability to carry out more complex and ambitious cell-like *** activities can provide insights into fundamental cell processes in simplified and idealized systems but could also have an applied impact in synthetic biology and biotechnology in the *** date,strategies for the bottom-up fabrication of micrometer scale life-like artificial cells have included stabilized water-in-oil droplets,giant unilamellar vesicles(GUV’s),hydrogels,and complex ***-in-oil droplets are a valuable and easy to produce model system for studying cell-like processes;however,the lack of a crowded interior can limit these artificial cells in mimicking life more *** membrane stabilized vesicles,such as GUV’s,have the additional membrane feature of cells but still lack a macromolecularly crowded ***-based artificial cells have a macromolecularly dense interior(although cross-linked)that better mimics cells,in addition to mechanical properties more similar to the viscoelasticity seen in cells but could be seen as being not dynamic in nature and limi

关键词： interior stabilized simplified

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A new method for precise optical measurements of the sub-micron height of levitation of droplet clusters

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Particuology 2024年第4期87卷 173-178页

作者： alexander A.Fedorets Eduard E.Kolmakov Leonid A.Dombrovsky University of Tyumen 6 Volodarskogo StTyumen625003Russia Engineering Faculty Ariel UniversityP0.B.3407000ArielIsrael Joint Institute for High Temperatures 17A Krasnokazarmennaya StMoscow111116Russia

Droplet clusters levitating over the locally heated water surface are considered a very promising phe-nomenon of microfluidics for the potential use of the droplets as unique microreactors for microbio-logical experiments.A new optical method is suggested for precise measurements of the sub-micron levitation height of single *** method is based on the analysis of variable colors of the inter-ference halo around the *** the first time,it is possible to measure the extremely low downward velocity of droplets,which grow due to steam *** velocity was found to be 5-8 nm/*** height of the levitation of various droplets just before their coalescence with a layer of water was also *** measurements might be used for the verification of sophisticated models for the droplet levitation to be developed for a very thin layer of humid air under the droplet when the Knudsen effect should be taken into account.

关键词： Precise measurements Interferometric method Droplet cluster Levitation height

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Atherosclerosis originating from childhood:Specific features

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Journal of biomedical Research 2024年第3期38卷 233-240页

作者： Anastasia V.Poznyak Alexey A.Yakovlev MikhailА.Popov Elena b.Zhigmitova Vasily N.Sukhorukov alexander N.Orekhov Institute for Atherosclerosis Research Moscow 121609Russia Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology Moscow 109240Russia Department of Cardiac Surgery Moscow Regional Research and Clinical InstituteMoscow 129110Russia Laboratory of Angiopathology Institute of General Pathology and PathophysiologyMoscow 125315Russia

Atherosclerosis is extremely ***,it is considered a disease of older people,who most often experience problems with the heart and blood *** much attention from the scientific community has been paid to studying the association between aging and atherosclerosis,as well as its consequences,there is evidence that atherosclerosis occurs at an early *** may form both during intrauterine development and in *** plays an important role in childhood atherosclerosis,along with previous infectious diseases and excess weight of both the child and the *** the present review,we examined the development of atherosclerosis and the prerequisites in childhood.

关键词： atherosclerosis childhood pediatrics cardiovascular disease

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On the two-layer high-level Green-Naghdi model in a general form

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Journal of Hydrodynamics 2024年第1期36卷 78-86页

作者： bin-bin Zhao Tian-yu Zhang Zhan Wang Wen-yang Duan alexander Chesnokov Natalia Shmakova College of Shipbuilding Engineering Harbin Engineering UniversityHarbin150001China Qingdao Innovation and Development Center of Harbin Engineering University Qingdao266000China Lavrentyev Institute of Hydrodynamics NovosibirskRussia

The traditional high-level Green-Naghdi(HLGN)model,which uses the polynomial as the shape function to approximate the variation of the horizontal-and vertical-velocity components along the vertical direction for each-fluid layer,can accurately describe the large-amplitude internal waves in a two-layer system for the shallow configuration(h_(2)/λ■1,h_(1)/λ■1).However,for the cases of the deep configuration(h_(2)/λ■1,h_(1)/λ=O(1)),higher-order polynomial is needed to approximate the variation of the velocity components along the vertical direction for the lower-fluid ***,however,introduces additional unknowns,leading to a significant increase in computational *** paper,for the first time,derives a general form of the HLGN model for a two-layer fluid system,where the general form of the shape function is used during the *** obtaining the general form of the two-layer HLGN equations,corresponding solutions can be obtained by determining the reasonable shape ***-amplitude internal solitary waves in a deep configuration are studied by use of two different HLGN *** of the two HLGN models shows that the polynomial as the shape function for the upper-fluid layer and the production of exponential and polynomial as the shape function for the lower-fluid layer is a good *** comparing with Euler’s solutions and the laboratory measurements,the accuracy of the two-layer HLGN model is verified.

关键词： High-level Green-Naghdi(HLGN)model general form internal waves deep configuration

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Diagnostic delay in inflammatory bowel diseases in a German population

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World Journal of Gastroenterology 2024年第29期30卷 3465-3478页

作者： Elisabeth blüthner Annalena Dehe Carsten büning britta Siegmund Matthias Prager Jochen Maul alexander Krannich Jan Preiß bertram Wiedenmann Florian Rieder Raneem Khedraki Frank Tacke Andreas Sturm Anja Schirbel Department of Hepatology and Gastroenterology Campus Virchow-Klinikum and Campus CharitéMitteCharité-Universitätsmedizin BerlinBerlin 10117Germany Department of Gastroenterology Vivantes Klinikum im FriedrichshainBerlin 10249Germany Department of Internal Medicine Krankenhaus WaldfriedeBerlin 14163Germany Department of Gastroenterology Infectious Diseases and RheumatologyCharité-Universitätsmedizin BerlinBerlin 12203Germany Praxis für Gastroenterologie berlin Zehlendorf Berlin 14195Germany Gastroenterologie am bayrischen Platz Berlin 10825Germany Clinical Trial Office Charité-Universitätsmedizin BerlinBerlin 10117Germany Department of Gastroenterology Diabetology and HepatologyVivantes Klinikum NeuköllnBerlin 10117Germany Department of Gastroenterology Hepatology and NutritionDigestive Diseases and Surgery InstituteThe Cleveland Clinic FoundationClevelandOH 44195United States Department of Internal Medicine DRK Kliniken Berlin WestendBerlin 14050Germany Gastroenterologie im Havelland Straße der EinheitFalkensee 14612Germany

bACKGROUND Early diagnosis is key to prevent bowel damage in inflammatory bowel disease(IbD).Risk factor analyses linked with delayed diagnosis in European IbD patients are scarce and no data in German IbD patients *** To identify risk factors leading to prolonged diagnostic time in a German IbD *** between 2012 and 2022,430 IbD patients from four berlin hospitals were enrolled in a prospective study and asked to complete a 16-item questionnaire to determine features of the path leading to IbD *** diagnostic time was defined as the time from symptom onset to consulting a physician(patient waiting time)and from first consultation to IbD diagnosis(physician diagnostic time).Univariate and multivariate analyses were performed to identify risk factors for each time *** The total diagnostic time was significantly longer in Crohn’s disease(CD)compared to ulcerative colitis(UC)patients(12.0 vs 4.0 mo;Pbetter outcome in these patients.

关键词： Diagnostic time Diagnostic delay Crohn’s disease Ulcerative colitis Germany

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Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α

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Synthetic and Systems biotechnology 2024年第3期9卷 416-424页

作者： Jeffrey L.Schloßhauer Lena Tholen alexander Korner Stefan Kubick Sofia Chatzopoulou Anja Honow Anne Zemella Fraunhofer Project Group PZ-Syn of the Fraunhofer Institute for Cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Fraunhofer Institute for Cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Faculty of Health Sciences Joint Faculty of the Brandenburg University of Technology Cottbus-SenftenbergThe Brandenburg Medical School Theodor FontaneUniversity of PotsdamPotsdamGermany Institute for Chemistry and biochemistry Laboratory of Protein BiochemistryFreie Universitat BerlinThielallee 6314195BerlinGermany Institute of biotechnology Technische Universit¨at BerlinStraße des 17.Juni 13510623BerlinGermany New/era/mabs GmbH August-Bebel-Str.8914482PotsdamGermany

Chinese hamster ovary(CHO)cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational ***,toxic proteins and membrane proteins are often difficult-to-express in living ***,cell-free protein synthesis can be *** study explores innovative strategies for enhancing the production of challenging proteins through the modification of CHO cells by investigating both,cell-based and cell-free approaches.A major result in our study involves the integration of a mutant eIF2 translation initiation factor and T7 RNA polymerase into CHO cell lysates for cell-free protein *** resulted in elevated yields,while eliminating the necessity for exogenous additions during cell-free production,thereby substantially enhancing ***,we explore the potential of the Rosa26 genomic site for the integration of T7 RNA polymerase and cell-based tetracycline-controlled protein *** findings provide promising advancements in bioproduction technologies,offering flexibility to switch between cell-free and cell-based protein production as needed.

关键词： Inducible expression CHO cells Cell-free protein synthesis CRISPR T7 RNA polymerase eIF2 Rosa26

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Impact of exercise on markers of b cell-related immunity:A systematic review

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Journal of Sport and Health Science 2024年第3期13卷 339-352,I0002页

作者： David Walzik Sergen belen Karen Wilisch Marie Kupjetz Silvana Kirschke Tobias Esser Niklas Joisten alexander Schenk Sebastian Proschinger Philipp Zimmer Division of Performance and Health(Sports Medicine) Institute for Sport and Sport ScienceTU Dortmund UniversityDortmund 44227Germany

background:b cells represent a crucial component of adaptive immunity that ensures long-term protection from infection by generating pathogen-specific *** alters b cell counts and immunoglobulin levels,but evidence-based conclusions on potential benefits for adaptive immunity are *** systematic review assessed current literatures on the impact of acute exercise and exercise training on b cells,immunoglobulins,and markers of secretory immunity in human ***:According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA)guidelines,MEDLINE,Web of Science,and Embase were searched on March 8,***-randomized controlled trials and crossover trials investigating the impact of acute exercise or exercise training on b cell counts and proportions,immunoglobulin levels,salivary flow rate,or secretory immunoglobulin A secretion rate were *** and reporting of exercise training studies were assessed using the Tool for the Assessment of Study Quality and reporting in *** characteristics,outcome measures,and statistically significant changes were summarized ***:Of the 67 eligible studies,22 applied acute exercise and 45 applied exercise *** included outcomes revealed significant alterations over time in acute exercise and exercise training context,but only a few investigations showed significant differences compared to control *** and plasma immunoglobulin A levels were most consistently increased in response to exercise ***:b cell-related outcomes are altered by acute exercise and exercise training,but evidence-based conclusions cannot be drawn with high confidence due to the large heterogeneity in populations and exercise ***-designed trials with large sample sizes are needed to clarify how exercise shapes b cell-related immunity.

关键词： Antibody b-lymphocyte Exercise Humoral immunity Immune system

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Elucidating elusive quaternary selenide EuCeCuSe3:Synthesis,crystal structure,properties and theoretical studies

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Journal of Rare Earths 2024年第1期42卷 163-171,I0006页

作者： Maxim V.Grigoriev Anna V.Ruseikina Maxim S.Molokeev Vladimir A.Chernyshev Aleksandr S.Aleksandrovsky alexander S.Krylov Svetlana N.Krylova Nikolai P.Shestakov Dmitriy A.Velikanov alexander A.Garmonov Alexey V.Matigorov Evgeny A.Ostapchuk Thomas Schleid Damir A.Safin Laboratory of Theory and Optimization of Chemical and Technological Processes University of TyumenVolodarskogo Str.6625003TyumenRussian Federation Kirensky Institute of Physics Federal Research Center KSC SB RASAkademgorodok 50 Bld.38660036KrasnoyarskRussian Federation Department of Physics Far Eastern State Transport UniversitySerysheva Str.47680021KrasnoyarskRussian Federation Institute of Natural Sciences and Mathematics Ural Federal University Named After the First President of Russia B.N.YeltsinMira Str.19620002EkaterinburgRussian Federation Department of Photonics and Laser Technology Siberian Federal UniversitySvobodnii Ave.79660079KrasnoyarskRussian Federation Institute of Physics and Technology University of TyumenVolodarskogo Str.6625003 TyumenRussian Federation Institute for Inorganic Chemistry University of StuttgartPfaffenwaldring 55D-70569StuttgartGermany Scientific and Educational and Innovation Center for Chemical and Pharmaceutical Technologies Ural Federal University named after the First President of Russia B.N.YeltsinMira Str.19620002 EkaterinburgRussian Federation University of Tyumen Volodarskogo Str.6625003TyumenRussian Federation

We report on the novel heterometallic quaternary selenide EuCeCuSe3,the fabrication of which has been a challenge until this *** structure of the reported selenide was elucidated from the powder X-ray diffraction data,which revealed the formation of EuCeCuSe3with excellent yield(96.7%)accompanied with a minor fraction of CeSe2(3.3%),and was best solved in orthorhombic space group Pnma with the baLaCuS3structural ***,the crystal structure of the title compound completes the row of the heterometallic quaternary selenides EuRECuSe3(RE=La,Pr,Nd,Sm,Eu,Gd,Tb,Dy,Ho,Er,Tm,Yb,Lu,Y),of which the cerium-based derivative exclusively belongs to the baLaCuS3structural *** distortion of the CuSe4polyhedron was compared for the whole series of EuRECuSe3compounds using theτ4-descriptor for four coordinated ions,which revealed the highest degree of distortion for the Ce3+-containing selenide,followed by the La3+-based ***,the crystallographic and geometrical parameters of the reported selenide were discussed in comparison to the Ce3+-based sulfides SrCeCuS3and *** initio calculations of the crystal structure,a phonon spectrum and elastic constants for the crystal of EuCeCuSe3were also *** types and wavenumbers of fundame ntal modes were determined and the involvement of ions participating in the phonon modes was *** experimental IR spectrum of the reported selenide was interpreted and found to be in agreement with the calculated *** experimental direct band gap of EuCeCuSe3was measured to be 1.36 eV that is consistent with the concept of its origin due to interband transitions between orbitals emerging mainly from 4f(valence band)and 5d(conduction band)levels of the Eu2+*** dependence of the Young’s modulus on the direction demonstrates the anisotropy of the elastic properties,while the Vickers hardness for EuCeCuSe3was calculated to be 5.2 ***,the title compound is paramagnetic above 4 K.

关键词： Quaternary selenide Synthesis Crystal structure Ab initio calculations Magnetic measurements Spectroscopy

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Transcriptional reprogramming during human osteoclast differentiation identifies regulators of osteoclast activity

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bone Research 2024年第1期12卷 180-198页

作者： Morten S.Hansen Kaja Madsen Maria Price Kent Søe Yasunori Omata Mario M.Zaiss Caroline M.Gorvin Morten Frost alexander Rauch Molecular Endocrinology Laboratory(KMEb) Department of EndocrinologyOdense University HospitalDK-5000 Odense CDenmark Department of Clinical Research Faculty of Health SciencesUniversity of Southern DenmarkDK-5000 Odense CDenmark Clinical Cell biology Pathology Research UnitDepartment of Clinical ResearchUniversity of Southern DenmarkDK-5000 Odense CDenmark Institute of Metabolism and Systems Research(IMSR)and Centre for Diabetes Endocrinology and Metabolism(CEDAM)University of BirminghamBirmingham B152TTUK Centre for Membrane Proteins and Receptors(COMPARE) Universities of Birmingham and NottinghamBirmingham B152TTUK Department of Molecular Medicine University of Southern DenmarkDK-5000 Odense CDenmark Department of Orthopedic Surgery Faculty of MedicineThe University of TokyoTokyo 113-8655Japan Department of Internal Medicine 3 Rheumatology and ImmunologyFriedrich-Alexander-University Erlangen-Nürnberg(FAU)and Universitätsklinikum ErlangenD-91054 ErlangenGermany Deutsches Zentrum Immuntherapie(DZI) Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum ErlangenD-91054 ErlangenGermany Steno Diabetes Center Odense Odense University HospitalDK-5000 Odense CDenmark

Enhanced osteoclastogenesis and osteoclast activity contribute to the development of osteoporosis,which is characterized by increased bone resorption and inadequate bone *** novel antiosteoporotic therapeutics are needed,understanding the genetic regulation of human osteoclastogenesis could help identify potential treatment *** study aimed to provide an overview of transcriptional reprogramming during human osteoclast *** were differentiated from CD14+monocytes from eight female *** sequencing during differentiation revealed 8980 differentially expressed genes grouped into eight temporal patterns conserved across *** patterns revealed distinct molecular functions associated with postmenopausal osteoporosis susceptibility genes based on RNA from iliac crest biopsies and bone mineral density *** analyses revealed mutual dependencies between temporal expression patterns and provided insight into subtype-specific transcriptional *** donor-specific expression patterns revealed genes at the monocyte stage,such as filamin b(FLNb)and oxidized low-density lipoprotein receptor 1(OLR1,encoding LOX-1),that are predictive of the resorptive activity of mature *** expression of differentially expressed G-protein coupled receptors was strong during osteoclast differentiation,and these receptors are associated with bone mineral density SNPs,suggesting that they play a pivotal role in osteoclast differentiation and *** regulatory effects of three differentially expressed G-protein coupled receptors were exemplified by in vitro pharmacological modulation of complement 5 A receptor 1(C5AR1),somatostatin receptor 2(SSTR2),and free fatty acid receptor 4(FFAR4/GPR120).Activating C5AR1 enhanced osteoclast formation,while activating SSTR2 decreased the resorptive activity of mature osteoclasts,and activating FFAR4 decreased both the number and resorptive activity of mature *** conclus

关键词： osteoclast programming identif

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Interstitial Doping of SnO_(2) Film with Li for Indium-Free Transparent Conductor

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Chinese Physics Letters 2024年第3期41卷 140-145页

作者：陈兴谦李昊臻陈伟梅增霞 alexander Azarov Andrej Kuznetsov 杜小龙 beijing National Laboratory for Condensed Matter Physics and Institute of PhysicsChinese Academy of SciencesBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China Department of Physics Centre for Materials Science and NanotechnologyUniversity of OsloP.O.Box 1048BlindernN-0316 OsloNorway Guangdong SinoPrime Technology Co. Ltd.Dongguan 523808China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China

SnO_(2)films exhibit significant potential as cost-effective and high electron mobility substitutes for In_(2)O_(3)*** this study,Li is incorporated into the interstitial site of the SnO_(2)lattice resulting in an exceptionally low resistivity of 2.028×10^(-3)Ω·cm along with a high carrier concentration of 1.398×10^(20)cm^(-3)and carrier mobility of 22.02 cm^(2)/V·s.

关键词： exceptional resistivity substitute

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