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Glutamate dehydrogenase and Na^+-K^+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

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Chinese Journal of Oceanology and Limnology 2011年第2期29卷 343-349页

作者：李二超 Leticia ARENA Gabriel LIZAMA Gabriela GAXIOLA Gerard CUZON Carlos ROSAS 陈立侨 alain van wormhoudt School of Life Science East China Normal University UMR5178 Station de Biologie Marine du Musum National d'Histoire NaturelleBP225Concarneau 29900France Unidad Multidisciplinaria de Docencia e Investigación Facultad de CienciasUniversidad Nacional Autónoma de MxicoPuerto de abrigo s/nSisal C.P.97350HunucmYucatnMexico IFREMER TahitiFrench Polynesia

Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp *** effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(***) were *** involved an examination of growth performance,glutamate dehydrogenase(GDH) and Na+-K+ ATPase mRNA expression,,and GDH activity in muscles and *** experimental diets were formulated,containing 25%,40%,and 50% dietary protein,and fed to the shrimp at a salinity of *** 20 days,no significant difference was observed in weight gain,though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein ***,shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5,respectively,and sampled at weeks 1 and *** fed with 40% protein at 25 in salinity(optimal conditions) were used as a *** of the salinities,shrimp fed with 50% dietary protein had significantly higher growth performance than other diets;no significant differences were found in comparison with the *** fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 *** salinity change also stimulated the hepatosomatic index,which increased in the first week and then recovered to a relatively normal level,as in the control,after 2 *** findings indicate that in white shrimp,the specific protein nutrient and energy demands related to ambient salinity change are associated with protein *** dietary protein level could improve the osmoregulation capacity of *** with more energy resources allocated to GDH activity and expression.

关键词： Litopenaeus vannamei gene expression glutamate dehydrogenase Na^+-K^+ ATPase protein salinity

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First steps on technological and genetic improvement of European abalone (<i>Haliotis tuberculata</i>) based on investigations in full-sib families

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Open Journal of Genetics 2013年第3期3卷 224-233页

作者： Valérie Roussel Julien Charreyron Sylvain Labarre alain van wormhoudt Sylvain Huchette France Haliotis Plouguerneau France MNHN UMR 7208 BOREA Station de Biologie Marine Concarneau France

The European abalone Haliotis tuberculata is of economical importance in Europe, and recently in France where most of the consumed abalones remained wild animals fished on the coast. Recently, the creation of hatcheries allowed the production of cultured animals, and aquaculture is in progress. To optimize selective breeding programs, different studies were performed on adults and on their progenies. First, ten adult families, assumed to be bi-parental and produced in 2007 were analyzed. As these families were developed at the beginning of the hatchery production, the parentage of the individual necessitated to be tested. The progenies parentage assignment was done by using eight microsatellite DNA loci. In fine, the heritability was estimated from the analysis of variance of seven full-sib families which include from 23 to 27 progenies. Heritability estimate was the higher for length, width and weight (0.37, 0.29 and 0.40 respectively). A high correlation was also shown between these heritable traits which can be useful during animal breeding. Shell color traits were also analyzed, using image treatment procedure. Two traits were selected according to the global color of the shell: red color and hue. The distribution of these traits evidenced a polygenic genetic control of shell color and measure of heritability evidenced a high to medium value for red color (Cr) and Hue(H) (0.33 and 0.20 respectively). No correlation was shown between growth and this parameter. In a second time, juveniles families produced in 2010 were studied from parents issued of the first selection. A correlation between size and density at juvenile stage was established but a statistical analysis demonstrated, by using density as co-variable a significant effect of family on size. In these conditions, growth is highly heritable (0.74). This study is a first step toward the use of genetic markers for selection, but also a step toward the breeding improvement of European abalone.

关键词： European Abalone Microsatellite Heritability Morphological Traits Shell Color Traits Juvenile

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Mycorrhizal fungi mitigate nitrogen losses of an experimental grassland by facilitating plant uptake and soil microbial immobilization

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Pedosphere 2024年第2期34卷 399-410页

作者： Yangyang JIA Marcel G.A.van DER HEIJDEN alain Y.VALZANO-HELD Markus JOCHER Florian WALDER Postdoctoral Mobile Station of Ecology College of Ecology and EnvironmentXinjiang UniversityÜrümqi 830046China Research Group Plant-Soil Interactions AgroscopeZürich 8046Switzerland Research Group Climate and Agriculture AgroscopeZürich 8046Switzerland Department of Plant and Microbial Biology University of ZürichZürich 8008Switzerland Research Group Soil Quality and Soil Use AgroscopeZürich 8046Switzerland

Nitrogen(N)is one of the most limited nutrients of terrestrial ecosystems,whose losses are prevented in tightly coupled cycles in finely tuned *** change-induced N enrichment through atmospheric deposition and application of vast amounts of fertilizer are now challenging the terrestrial N *** mycorrhizal fungi(AMF)are known drivers of plant-soil nutrient fluxes,but a comprehensive assessment of AMF involvement in N cycling under global change is still ***,we simulated N enrichment by fertilization(low/high)in experimental grassland microcosms under greenhouse conditions in the presence or absence of AMF and continuously monitored different N pathways over nine *** found that high N enrichment by fertilization decreased the relative abundance of legumes and the plant species dominating the plant community changed from grasses to forbs in the presence of AMF,based on aboveground *** presence of AMF always maintained plant N:phosphorus(P)ratios between 14 and 16,no matter how the soil N availability *** in plant N:P ratios due to the increased plant N and P uptake might thus be a primary pathway of AMF altering plant community ***,we constructed a comprehensive picture of AMF’s role in N cycling,highlighting that AMF reduced N losses primarily by mitigating N leaching,while N_(2)O emissions played a marginal *** mycorrhizal fungi reduced N_(2)O emissions directly through the promotion of N_(2)O-consuming *** underlying mechanism for reducing N leaching is mainly the AMF-mediated improved nutrient uptake and AMF-associated microbial *** results indicate that synergies between AMF and other soil microorganisms cannot be ignored in N cycling and that the integral role of AMF in N cycling terrestrial ecosystems can buffer the upcoming global changes.

关键词： N cycling N enrichment N uptake N:P ratio plant community structure symbiotic soil fungi

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Analysis of Risk Factors for Early Mortality in Surgical Shunt Palliation:Time for a Change?

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Congenital Heart Disease 2023年第5期18卷 539-550页

作者： François-Xavier van Vyve Karlien Carbonez Jelena Hubrechts Geoffroy de Beco Jean ERubay Mona Momeni Thierry Detaille alain J.Poncelet Department of Cardiovascular and Thoracic Surgery Universitécatholique de Louvain(UCLouvain)-Cliniques Universitaires Saint-LucBrusselsBelgium Department of Pediatric Cardiology Universitécatholique de Louvain(UCLouvain)-Cliniques Universitaires Saint-LucBrusselsBelgium Department of Anesthesiology Universitécatholique de Louvain(UCLouvain)-Cliniques Universitaires Saint-LucBrusselsBelgium Department of Pediatric Intensive Care Universitécatholique de Louvain(UCLouvain)-Cliniques Universitaires Saint-LucBrusselsBelgium

Objectives:Over the last decade,neonatal repair has been advocated for many congenital heart ***,specific subgroups of complex congenital heart disease still require temporary palliation for which both surgical and endovascular techniques are currently *** reviewed our institutional experience with shunt palliation with an emphasis on risk factors for early ***:This is a single-center retrospective study on 175 patients undergoing surgery for central shunt or modified Blalock-Taussig *** data were extracted from a prospectively collected computerized *** identified risk factors for early mortality by uni-and multi-variable *** data were censored at the time of death or shunt take-down ***:Mean age and weight at surgery were 24 days(IQR[7–95])and 3.4 kg(IQR[2.9–4.8]),respectively,with 96 neonates(55%).Most patients had a biventricular heart disease(115 patients,66%),and 51 patients(29.1%)had univentricular heart *** was performed in 129 patients(74%).Cardiopulmonary bypass was used in 23 patients(13%).The median intensive care and overall length of stay were 4 days(IQR[2–9])and 18 days(IQR[13–29]),***-hospital mortality was 8.6%(15/175).By multivariable regression analysis,prematurity(HR 5.6[2.1–14.7]),CPB use(HR 6.7[2.2–18.6]),unplanned<30-day reoperation(HR 3.5[1.2–10])or catheterization(HR 4.5[1.2–16.9])were all significant predictors of early ***:Procedural-related mortality remains high(8.6%)in surgical shunt *** patients with prematurity,low weight at birth,or if the use of cardiopulmonary bypass is contemplated,alternative endovascular techniques of palliation should be considered together with longitudinal follow-up studies.

关键词： Congenital heart disease cyanosis palliation neonates outcome

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Physical and chemical changes of rapeseed meal proteins during toasting and their effects on in vitro digestibility

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Journal of Animal Science and Biotechnology 2017年第2期8卷 417-427页

作者： Sergio Salazar-Villanea Erik M.A.M.Bruininx Harry Gruppen Wouter H.Hendriks Patrick Carré alain Quinsac Antonius F.B.van der Poel Wageningen Livestock Research P.O.Box 3386700 AH WageningenThe Netherlands Animal Nutrition Group Wageningen University&ResearchP.O.Box 3386700 AH WageningenThe Netherlands Agfifirm Innovation Center Royal Dutch Agrifirm GroupP.O.Box 200187302 HA ApeldoornThe Netherlands Laboratory of Food Chemistry Wageningen University&ResearchP.O.Box 176700 AA WageningenThe Netherlands CREOL/OLEAD 11 rue MongeParc Industriel33600 PessacFrance Terres Inovia 11 rue MongeParc Industriel33600 PessacFrance

Background： Toasting during the production of rapeseed meal（RSM） decreases ileal crude protein（CP） and amino acid（AA） digestibility. The mechanisms that determine the decrease in digestibility have not been fully elucidated. A high protein quality, low-denatured, RSM was produced and toasted up to 120 min, with samples taken every 20 min. The aim of this study was to characterize secondary structure and chemical changes of proteins and glucosinolates occurring during toasting of RSM and the effects on its in vitro CP ***： The decrease in protein solubility and the increase of intermolecular β-sheets with increasing toasting time were indications of protein aggregation. The contents of NDF and ADIN increased with increasing toasting *** of arginine, lysine and O-methylisourea reactive lysine（OMIU-RL） linearly decreased with increasing toasting time, with a larger decrease of OMIU-RL than lysine. First-order reactions calculated from the measured parameters show that glucosinolates were degraded faster than lysine, OMIU-RL and arginine and that physical changes to proteins seem to occur before chemical changes during toasting. Despite the drastic physical and chemical changes noticed on the proteins, the coefficient of in vitro CP digestibility ranged from 0.776 to 0.750 and there were no effects on the extent of protein hydrolysis after 120 min. In contrast, the rate of protein hydrolysis linearly decreased with increasing toasting time, which was largely correlated to the decrease in protein solubility, lysine and OMIU-RL observed. Rate of protein hydrolysis was more than 2-fold higher for the untoasted RSM compared to the 120 min toasted ***： Increasing the toasting time for the production of RSM causes physical and chemical changes to the proteins that decrease the rate of protein hydrolysis. The observed decrease in the rate of protein hydrolysis could impact protein digestion and utilization.

关键词： Hydrolysis rate In vitro protein digestibility Rapeseed meal Reactive lysine Secondary structure

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Analysis of Pulmonary Arteries Growth after Initial Shunt Palliation in Neonates and Infants

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Congenital Heart Disease 2023年第5期18卷 525-537页

作者： François-Xavier van Vyve Karlien Carbonez Geoffroy de Beco Stéphane Moniotte Jean ERubay Mona Momeni Laurent Houtekie alain J.Poncelet Department of Cardiovascular and Thoracic Surgery Universitécatholique de Louvain(UCLouvain)—Cliniques Universitaires Saint-LucBrusselsB-1200Belgium Department of Pediatric Cardiology Universitécatholique de Louvain(UCLouvain)—Cliniques Universitaires Saint-LucBrusselsB-1200Belgium Department of Anesthesiology Universitécatholique de Louvain(UCLouvain)—Cliniques Universitaires Saint-LucBrusselsB-1200Belgium Department of Pediatric Intensive Care Universitécatholique de Louvain(UCLouvain)—Cliniques Universitaires Saint-LucBrusselsB-1200Belgium

Objective:Despite increasing enthusiasm for neonatal repair,patients with ductal-dependent circulation(pulmonary/systemic)or restrictive pulmonary blood flow still require initial *** stenting has emerged as an endovascular approach whereas modified-Blalock-Taussig and central shunt remain surgical *** this study,we analyzed the relationship between pulmonary artery growth,sites of shunt connection,or antegrade pulmonary blood flow in surgically placed *** need for secondary catheter-based interventions or pulmonary arterioplasty was also ***:A retrospective single-center study analyzing 175 patients undergoing surgery for a central or modified-Blalock-Taussig *** growth variables were right pulmonary artery/left pulmonary artery diameters/Z scores,the indexed sum area(right pulmonary artery+left pulmonary artery),and the pulmonary symmetry *** imaging modalities were used:angiography,computed tomography,and ***:At baseline,pulmonary arteries were larger in patients with antegrade pulmonary blood flow(Nakata index 137 vs.114,p=0.047)as well as in patients receiving a modified-Blalock-Taussig shunt(Nakata index 138 vs.84,p<0.001).At the time of shunt takedown,both the right pulmonary artery and left pulmonary artery had normalized their *** Nakata index increased from 134 to 233 mm^(2)/m^(2)(p<0.001).The pulmonary artery index remained stable(0.86)over *** the inter-stage period,shunt-related pulmonary artery stenosis and juxta-ductal stenosis were diagnosed in 16(10%)and 17 patients(11%),***:Surgical shunt palliation allows normal pulmonary artery *** artery stenosis was either shunt-related(10%)or secondary to juxta-ductal stenosis(11%).Close echographic follow-up allows early diagnosis and treatment of juxta-ductal stenosis.

关键词： Congenital heart disease cyanosis palliation neonates outcome

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Characterising Vertical Redistribution on Irrigated Furrows in the Tukulu Soil

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Journal of Agricultural Science and Technology(A) 2013年第7期3卷 542-560页

作者： Sabelo Sicelo Wesley Mavimbela Leon Daniel van Rensburg alain Cloot Department of Soil Crop and Climate Sciences University of the Free State Bloemfontein 9300 South Africa Department of Mathematics and Applied Mathematics University of the Free State Bloemfontein 9300 South Africa

Subsurface soil water redistribution on the South African Tukulu, also referred as the Cutanic Luvisols in other countries, was evaluated following single run irrigation （20, 40, 80 and 160 L/min inflow rates） in 90 m furrows. Changes in soil water content （SWC） at three horizons were monitored using neutron water meter. Measurements were made every 10 m starting 5 m from the furrow inlet for 455 h. HYDRUS-2D software was used to estimate soil hydraulic parameters through inverse optimization algorithms for redistribution at the inlet, midpoint and furrow end. Optimized model parameters compared with initial estimates recorded satisfactory agreement between measured and predicted soil water content, despite spatial variability. Effective hydraulic conductivity （Keff） for 0-600 mm and 0-850 mm profile flow domains demonstrated linearity with SWC although inconsistencies under field conditions were inevitable. The underlying layer restricted gravity and augmented redistribution with Keff assuming a steeper gradient than normal. Conversion of KCff and soil water content into a ratio assisted in quantifying rate of redistribution at 0-600 mm and 0-850 mm profile depth. Vertical redistribution was found to be limited within the upper 600 mm depth thus providing the opportunity to develop furrow irrigation with confidence that water productivity is optimized.

关键词： Soil water redistribution inflow rates effective unsaturated hydraulic conductivity infiltrated depth HYDRUS-2D.

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Determining the Optimum Inflow Rates for Micro-flood Irrigation on the Tukulu Soil

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Journal of Agricultural Science and Technology(A) 2014年第8期4卷 633-649页

The performance of micro flood irrigation （MFI） under different inflow rates was evaluated on 90 m closed ended furrows in the South African Tukulu soil. A single irrigation was used to characterise the surface and subsurface soil water distribution from the 20, 40, 80 and 160 L/min inflow rates treatments. Neutron access tubes were installed to a depth of 1 m at every 10 m distance interval starting at 5 m from the furrow inlet. Soil water content measurements were taken using the WaterMan neutron water meter. The HYDRUS-2D software was also used to provide insight on irrigated furrows soil water content and subsurface water distribution. The 20 L/min produced a stream flow that could advance up to the 60 m furrow distance. The stream flow from the rest of the inflow rates were able to reach the furrow end with the 180 L/min recording the fastest advance time of 23 min. The 20 L/min and 40 L/min had recession period of less than 7 min while the 80 L/rain and 160 L/min lasted more than an hour. Distribution uniformity （DU） at longer furrow distances was the highest from the 80 L/min and 160 L/min with the 20 L/min and 40 L/min recorded similar performances at shorter distances. The 40 L/rain was one of the smaller inflow rates that recorded the highest DU of 0.96 for the generated average infiltrated depth of the 30 m long furrow and therefore should be adopted for furrow distances of less than 60 m on the Tukulu soil.

关键词： Short furrow irrigation infiltrated depth HYDRUS-2D distribution uniformity.

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Missing metastases as a model to challenge current therapeutic algorithms in colorectal liver metastases

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World Journal of Gastroenterology 2016年第15期22卷 3937-3944页

作者： Valerio Lucidi alain Hendlisz Jean-Luc van Laethem Vincent Donckier Department of Abdominal Surgery H?pital Erasme Centre de Chirurgie Hépato-Biliaire de l’ULB Université Libre de Bruxelles Department of Medical Oncology Institut Jules Bordet Université Libre de Bruxelles Department of Gastroenterology H?pital Erasme Université Libre de Bruxelles Department of Surgery Institut Jules Bordet Department of Abdominal Surgery H?pital Erasme Centre de Chirurgie Hépato-Biliaire de l’ULB Université Libre de Bruxelles

In oncosurgical approach to colorectal liver metastases, surgery remains considered as the only potentially curative option, while chemotherapy alone represents a strictly palliative treatment. However, missing metastases, defined as metastases disappearing after chemotherapy, represent a unique model to evaluate the curative potential of chemotherapy and to challenge current therapeutic algorithms. We reviewed recent series on missing colorectal liver metastases to evaluate incidence of this phenomenon, predictive factors and rates of cure defined by complete pathologic response in resected missing metastases and sustained clinical response when they were left unresected. According to the progresses in the efficacy of chemotherapeutic regimen, the incidence of missing liver metastases regularly increases these last years. Main predictive factors are small tumor size, low marker level, duration of chemotherapy, and use of intra-arterial chemotherapy. Initial series showed low rates of complete pathologic response in resected missing metastases and high recurrence rates when unresected. However, recent reports describe complete pathologic responses and sustained clinical responses reaching 50%, suggesting that chemotherapy could be curative in some cases. Accordingly, in case of missing colorectal liver metastases, the classical recommendation to resect initial tumor sites might have become partially obsolete. Furthermore, the curative effect of chemotherapy in selected cases could lead to a change of paradigm in patients with unresectable liver-only metastases, using intensive first-line chemotherapy to intentionally induce missing metastases, followed by adjuvant surgery on remnant chemoresistant tumors and close surveillance of initial sites that have been left unresected.

关键词： Colorectal Liver Metastases,Surgery Chemotherapy Missing

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整合量测技术的新应用和定义

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集成电路应用 2006年第8期23卷 29-31页

作者： alain Diebold Sematech Bradley van Eck ISMI

虽然工艺和良率控制离不开量测技术,但对于量测、整合量测、原位量测、原位传感器、工艺和设备传感器却没有形成统一的定义,因此存在严重的混淆。

关键词：量测技术定义传感器工艺原位

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