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Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第3期14卷 216-223页

作者： Hai-hong ZHANG ai-zhen CA Xue-ming WEI Li DING Feng-zhi LI ai-ming zheng Da-jiang Dai Rong-rong HUANG Hou-jun CAO Hai-yang ZHOU Jian-mei WANG Xue-jing WANG Wei SHI Heng ZHU Xiao-ying YUAN Lin CHEN Department of General Surgery General Hospital of the Air ForcePLA Department of General Surgery Chinese PLA General Hospital Department of Cell Biology Institute of Basic Medical SciencesAcademy of Military Medical Sciences

Objective:Side population(SP) cells may play a crucial role in tumorigenesis and the recurrence of *** kinds of cell lines and tissues have demonstrated the presence of SP cells,including several gastric cancer cell *** study is aimed to identify the cancer stem-like cells in the SP of gastric cancer cell line ***:We used fluorescence activated cell sorting(FACS) to sort SP cells in the human gastric carcinoma cell line MKN-45(cells labeled with Hoechst 33342) and then characterized the cancer stem-like properties of SP ***:This study found that the SP cells had higher clone formation efficiency than major population(MP) *** stemness-related gene expression profiles,including OCT-4,SOX-2,NANOG,CD44,and adenosine triphosphate(ATP)-binding cassette transporters gene ABCG2,were tested in SP and MP cells using quantitative real-time reverse transcription polymerase chain reaction(RT-PCR).Western blot was used to show the difference of protein expression between SP and MP *** results show that there was significantly higher protein expression in SP cells than in MP *** inoculated into non-obese diabetic/severe combined immunodeficiency(NOD/SCID) mice,SP cells show higher tumorigenesis tendency than MP ***:These results indicate that SP cells possess cancer stem cell properties and prove that SP cells from MKN-45 are gastric cancer stem-like cells.

关键词： ATP-binding cassette transporters Side population cells Stem cells Benzimidazole (Hoechst 33342),Stomach neoplasm

Two-Staged Method for Ice Channel Identification Based on Image Segmentation and Corner Point Regression

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China Ocean Engineering 2024年第2期38卷 313-325页

作者： DONG Wen-bo ZHOU Li DING Shi-feng WANG ai-ming Cai Jin-yan School of Naval Architecture and Ocean Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Identification of the ice channel is the basic technology for developing intelligent ships in ice-covered waters,which is important to ensure the safety and economy of *** the Arctic,merchant ships with low ice class often navigate in channels opened up by *** in the ice channel often depends on good maneuverability skills and abundant experience from the captain to a large *** ship may get stuck if steered into ice fields off the *** this circumstance,it is very important to study how to identify the boundary lines of ice channels with a reliable *** this paper,a two-staged ice channel identification method is developed based on image segmentation and corner point *** first stage employs the image segmentation method to extract channel *** the second stage,an intelligent corner regression network is proposed to extract the channel boundary lines from the channel region.A non-intelligent angle-based filtering and clustering method is proposed and compared with corner point regression *** training and evaluation of the segmentation method and corner regression network are carried out on the synthetic and real ice channel *** evaluation results show that the accuracy of the method using the corner point regression network in the second stage is achieved as high as 73.33%on the synthetic ice channel dataset and 70.66%on the real ice channel dataset,and the processing speed can reach up to 14.58frames per second.

关键词： ice channel ship navigation identification image segmentation corner point regression

Synergistic effects of Pleistocene geological and climatic events on complex phylogeographic history of widespread sympatric species of Megaloptera in East Asia

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Zoological Research 2024年第5期45卷 1131-1146页

作者： ai-Li Lin ming-ming Zou Li-Jun Cao Fumio Hayashi Ding Yang Xing-Yue Liu Department of Entomology China Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University SanyaHainan 572025China International Joint Laboratory of Taxonomy and Systematic Evolution of Insecta Henan Institute of Science and TechnologyXinxiangHenan 453003China Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China Department of Biology Tokyo Metropolitan UniversityHachioji-shiTokyo 192-0397Japan

Unraveling the phylogeographic histories of species remains a key endeavor for comprehending the evolutionary processes contributing to the rich biodiversity and high endemism found in East *** this study,we explored the phylogeographic patterns and demographic histories of three endemic fishfly and dobsonfly species(Neochauliodes formosanus,Protohermes costalis,and Neoneuromus orientalis)belonging to the holometabolan order *** species,which share a broad and largely overlapping distribution,were analyzed using comprehensive mitogenomic *** findings revealed a consistent influence of vicariance on the population isolation of *** and *** between Hainan,Taiwan,and the East Asian mainland during the early Pleistocene,potentially hindering subsequent colonization of the later diverged *** to these ***,we unveiled the dual function of the major mountain ranges in East Asia,serving both as barriers and conduits,in shaping the population structure of all three ***,we demonstrated that these co-distributed species originated from Southwest,Southern,and eastern Central China,respectively,then subsequently migrated along multi-directional routes,leading to their sympatric distribution on the East Asian ***,our results highlighted the significance of Pleistocene land bridges along the eastern coast of East Asia in facilitating the dispersal of mountain-dwelling insects with low dispersal ***,this study provides novel insight into the synergistic impact of Pleistocene geological and climatic events in shaping the diversity and distribution of aquatic insects in East Asia.

关键词： Comparative phylogeography Island biogeography Pleistocene glaciation Corydalidae East Asia

Epigenetic silencing schlafen-11 sensitizes esophageal cancer to ATM inhibitor

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2060-2073页

作者： Jing Zhou Mei-Ying Zhang ai-ai Gao Cheng Zhu Tao He James G Herman ming-Zhou Guo School of Medicine NanKai UniversityTianjin 300071China Department of Gastroenterology and Hepatology The First Medical CenterChinese PLA General HospitalBeijing 100853China Departments of Pathology Characteristic Medical Center of The Chinese People’s Armed Police ForceTianjin 300162China The Hillman Cancer Center University of Pittsburgh Cancer InstitutePittsburghPA 15213United States National Key Laboratory of Kidney Diseases Chinese PLA General HospitalBeijing 100853China

BACKGROUND Targeting DNA damage response(DDR)pathway is a cutting-edge *** has been reported that Schlafen-11(SLFN11)contributes to increase chemosensitivity by participating in ***,the detailed mechanism is *** To investigate the role of SLFN11 in DDR and the application of synthetic lethal in esophageal cancer with SLFN11 *** To reach the purpose,eight esophageal squamous carcinoma cell lines,142 esophageal dysplasia(ED)and 1007 primary esophageal squamous cell carcinoma(ESCC)samples and various techniques were utilized,including methylationspecific polymerase chain reaction,CRISPR/Cas9 technique,Western blot,colony formation assay,and xenograft mouse *** Methylation of SLFN11 was exhibited in 9.15%of(13/142)ED and 25.62%of primary(258/1007)ESCC cases,and its expression was regulated by promoter region ***11 methylation was significantly associated with tumor differentiation and tumor size(both P<0.05).However,no significant associations were observed between promoter region methylation and age,gender,smoking,alcohol consumption,TNM stage,or lymph node *** DNA damaged model induced by low dose cisplatin,SLFN11 was found to activate non-homologous end-joining and ATR/CHK1 signaling pathways,while inhibiting the ATM/CHK2 signaling *** silencing of SLFN11 was found to sensitize the ESCC cells to ATM inhibitor(AZD0156),both in vitro and in *** SLFN11 is frequently methylated in human *** of SLFN11 is sensitive marker of ATM inhibitor in ESCC.

关键词： Schlafen-11 Esophageal squamous cell carcinoma DNA methylation Synthetic lethality AZD0156

Pathogenesis of chronic enteropathy associated with the SLCO2A1 gene:Hypotheses and conundrums

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World Journal of Gastroenterology 2024年第19期30卷 2505-2511页

作者： Zhi-Xin Xie Yue Li ai-ming Yang Dong Wu Qiang Wang State Key Laboratory of Complex Severe and Rare Diseases Department of GastroenterologyPeking Union Medical College HospitalChinese Academy of Medical SciencesBeijing 100730China Department of Clinical Medicine Peking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China

Chronic enteropathy associated with the SLCO2A1 gene(CEAS)is a complex gastroenterological condition characterized by multiple ulcers in the small intestine with chronic bleeding and protein *** review explores the potential mechanisms underlying the pathogenesis of CEAS,focusing on the role of SLCO2A1-encoded prostaglandin transporter OATP2A1 and its impact on prostaglandin E2(PGE2)*** have suggested that elevated PGE2 levels contribute to mucosal damage,inflammation,and disruption of the intestinal *** effects of PGE2 on macrophage activation and Maxi-Cl channel functionality,as well as its interaction with nonsteroidal anti-inflammatory drugs play crucial roles in the progression of *** the balance between its protective and pro-inflammatory effects and the complex interactions within the gastrointestinal tract can shed light on potential therapeutic targets for CEAS and guide the development of novel,targeted therapies.

关键词： SLCO2A1 Prostaglandin E2 Chronic enteropathy associated with the SLCO2A1 gene Small intestine Macrophage

Mechanism of Qili Qiangxin Capsule for Heart Failure Based on miR133a-Endoplasmic Reticulum Stress

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Chinese Journal of Integrative Medicine 2024年第5期30卷 398-407页

作者： JI Xiao-di YANG Ding CUI Xi-yuan LOU Li-xia NIE Bo ZHAO Jiu-li ZHAO ming-jing WU ai-ming Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine Key Laboratory of Chinese Internal Medicine of Ministry of Education and BeijingBeijing 100700China Department of Traditional Chinese Medicine Fuwai HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100037China

Objective:To investigate the pharmacological mechanism of Qili Qiangxin Capsule(QLQX)improvement of heart failure(HF)based on miR133a-endoplasmic reticulum stress(ERS)***:A left coronary artery ligation-induced HF after myocardial infarction model was used in this *** were randomly assigned to the sham group,the model group,the QLQX group[0.32 g/(kg·d)],and the captopril group[2.25 mg/(kg·d)],15 rats per group,followed by 4 weeks of *** function such as left ventricular ejection fraction(EF),fractional shortening(FS),left ventricular systolic pressure(LVSP),left ventricular end diastolic pressure(LVEDP),the maximal rate of increase of left ventricular pressure(+dp/dt max),and the maximal rate of decrease of left ventricular pressure(–dp/dt max)were monitored by echocardiography and *** and eosin(HE)and Masson stainings were used to visualize pathological changes in myocardial *** m RNA expression of mi R133a,glucose-regulated protein78(GRP78),inositol-requiring enzyme 1(IRE1),activating transcription factor 6(ATF6),X-box binding protein1(XBP1),C/EBP homologous protein(CHOP)and Caspase 12 were detected by *** protein expression of GRP78,p-IRE1/IRE1ratio,cleaved-ATF6,XBP1-s(the spliced form of XBP1),CHOP and Caspase 12 were detected by Western *** T-mediated d UTP nick-end labeling(TUNEL)staining was used to detect the rate of ***:QLQX significantly improved cardiac function as evidenced by increased EF,FS,LVSP,+dp/dt max,-dp/dt max,and decreased LVEDP(Paining showed that QLQX ameliorated cardiac pathologic damage to some *** staining indicated that QLQX significantly reduced collagen volume fraction in myocardial tissue(P<0.01).Results from RT-PCR and Western blot showed that QLQX significantly increased the expression of mi R133a and inhibited the m RNA expressions of GRP78,IRE1,ATF6 and XBP1,as well as decreased the protein expressions of GRP78,cleave

关键词： heart failure Qili Qiangxin Capsule miR133a endoplasmic reticulum stress apoptosis

Resection of polyps involving the appendiceal orifice by combined endo-laparoscopic surgery: Two case reports

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World Journal of Gastrointestinal Surgery 2024年第6期16卷 1948-1952页

作者： Yue-Yi Zhang Jun-Yang Lu Qiang Wang ai-ming Yang Department of Gastroenterology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of General Surgery State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

BACKGROUND The management of polyps involving the appendiceal orifice(AO)presents notable *** resection is frequently hindered by operational complexities,a heightened risk of incomplete removal,and an elevated risk of procedural complications,including ***,surgical resection may entail unnecessary excision of intestinal segments,leading to potential *** SUMMARY Here,we reported two patients who presented with polyps deeply situated within the AO,with indistinct boundaries making it challenging to ensure completeness using traditional endoscopic *** overcome these challenges,we em-ployed combined endo-laparoscopic surgery(CELS),achieving curative resection without postoperative *** The application of CELS in managing polyps involving the AO is emerging as a safe and effective treatment modality.

关键词： Polyps Laterally spreading tumor Appendiceal orifice Endoscopic resection Combined endo-laparoscopic surgery Case report

Pricing Guaranteed Minimum Death Benefits with Dollar Cost Averaging Under Time-changed Le′vy Models

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Acta Mathematicae Applicatae Sinica 2024年

作者： Jia-ming WANG Mei-qiao ai Zhi-min ZHANG College of Mathematics and Statistics Chongqing University

In this paper, we propose an efficient and accurate method for pricing Guaranteed Minimum Death Benefit (GMDB) under time-changed Le′vy processes. Suppose that the GMDB payoff depends on a dollar cost averaging (DCA) style periodic investment, and the activity rate process in stochastic time change is modeled by a square-root process. We develop a recursive method to derive the closed form valuation formula by using the frame duality projection method. Numerical examples are reported for demonstrating the effectiveness of our approach and illustrating the interplay between contract parameters and the valuation.

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化合物库的组合技巧和组合合成

有机化学 1998年第2期18卷 186-191页

作者：喻爱明杨华铮南开大学元素有机化学研究所天津300071

从化合物库的组合技巧和组合合成方法两个方面探讨了组合化学及其在药物开发中的应用。

关键词：组合化学化合物库组合合成药物开发药物筛选