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Regenerative peripheral nerve interface prevents neuroma formation after peripheral nerve transection

Neural Regeneration Research 2023年第4期18卷 814-818页

作者： Zheng Wang xin-Zeyu Yi ai-xi yu Department of Orthopedics Trauma and Microsurgery Zhongnan Hospital of Wuhan UniversityWuhanHubei ProvinceChina

Neuroma formation after peripheral nerve transection often leads to severe neuropathic *** peripheral nerve interface has been shown to reduce painful neuroma in the ***,no reports have investigated the underlying mechanisms,and no comparative animal studies on regenerative peripheral nerve interface and other means of neuroma prevention have been conducted to *** this study,we established a rat model of left sciatic nerve transfection,and subsequently interfered with the model using the regenerative peripheral nerve interface or proximal nerve stump implantation inside a fully innervated *** showed that,compared with rats subjected to nerve stump implantation inside the muscle,rats subjected to regenerative peripheral nerve interface intervention showed greater inhibition of the proliferation of collagenous fibers and irregular regenerated axons,lower expressions of the fibrosis markerα-smooth muscle actin and the inflammatory marker sigma-1 receptor in the proximal nerve stump,lower autophagy behaviors,lower expressions of c-fos and substance P,higher expression of glial cell line-derived neurotrophic factor in the ipsilateral dorsal root *** findings suggested that regenerative peripheral nerve interface inhibits peripheral nerve injury-induced neuroma formation and neuropathic pain possibly via the upregulation of the expression of glial cell line-derived neurotrophic factor in the dorsal root ganglia and reducing neuroinflammation in the nerve stump.

关键词： autotomy dorsal root ganglia glial cell line-derived neurotrophic factor nerve injury neuropathic pain peripheral nerve regeneration regenerative peripheral nerve interface retrograde axonal transport traumatic neuroma

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Bioengineered miR-124-3p prodrug selectively alters the proteome of human carcinoma cells to control multiple cellular components and lung metastasis in vivo

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Acta Pharmaceutica Sinica B 2021年第12期11卷 3950-3965页

作者： Linglong Deng Hannah Petrek Mei-Juan Tu Neelu Batra ai-xi yu ai-Ming yu Department of Orthopaedic Trauma and Microsurgery Zhongnan Hospital of Wuhan UniversityWuhan 430072China Department of Biochemistry and Molecular Medicine UC Davis School of MedicineSacramentoCA 95817USA

With the understanding of microRNA(miRNA or miR) functions in tumor initiation,progression,and metastasis,efforts are underway to develop new miRNA-based *** recently,we demonstrated effectiveness of a novel humanized bioengineered miR-124-3 p prodrug in controlling spontaneous lung metastasis in mouse *** study was to investigate the molecular and cellular mechanisms by which miR-124-3 p controls tumor *** study identified a set of proteins selectively and significantly downregulated by bioengineered miR-124-3 p in A549 cells,which were assembled into multiple cellular components critical for metastatic *** them,plectin(PLEC) was verified as a new direct target for miR-124-3 p that links cytoskeleton components and *** miR-124-3 p-treated lung cancer and osteosarcoma cells,protein levels of vimentin,talin 1(TLN1),integrin beta-1(ITGB1),IQ motif containing GTPase activating protein 1(IQGAP1),cadherin2 or N-cadherin(CDH2),and junctional adhesion molecule A(F11 R or JAMA or JAM1) decreased,causing remodeling of cytoskeletons and disruption of cell-cell ***,miR-124-3 p sharply suppressed the formation of focal adhesion plaques,leading to reduced cell adhesion ***,efficacy and safety of biologic miR-124-3 p therapy was established in an aggressive experimental metastasis mouse model in *** results connect miR-124-3 p-PLEC signaling to other elements in the control of cytoskeleton,cell junctions,and adhesion essential for cancer cell invasion and extravasation towards metastasis,and support the promise of miR-124 therapy.

关键词： RNA therapy MiR-124-3p Metastasis Proteomics Junctions Cell adhesion Bioengineer Imaging

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Bioengineered miR-328-3p modulates GLUT1-mediated glucose uptake and metabolism to exert synergistic antiproliferative effects with chemotherapeutics

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Acta Pharmaceutica Sinica B 2020年第1期10卷 159-170页

作者： Wanrong Yi Mei-Juan Tu Zhenzhen Liu Chao Zhang Neelu Batra ai-xi yu ai-Ming yu Department of Orthopaedic Trauma and Microsurgery Zhongnan Hospital of Wuhan UniversityWuhan 430072China Department of Biochemistry&Molecular Medicine UC Davis School of MedicineSacramento 95817CAUSA

MicroRNAs(miRNAs or miRs)are small noncoding RNAs derived from genome to control target gene *** we have developed a novel platform permitting high-yield production of bioengineered miRNA agents(BERA).This study is to produce and utilize novel fully-humanized BERA/miR-3’28-3p molecule(hBERA/miR-3’28)to delineate the role of miR-328-3p in controlling nutrient uptake essential for cell *** first demonstrated successful high-level expression of hBERA/miR-328 in bacteria and purification to high degree of homogeneity(>98%).Biologic miR-328-3p prodrug was selectively processed to miR-328-3p to suppress the growth of highly-proliferative human osteosarcoma(OS)*** glucose transporter protein type 1,gene symbol solute carrier family 2 member 1(GUJTHSLC2A1),we identified and verified large neutral amino acid transporter 1,gene symbol solute carrier family 7 member 5(LPAT1/SLC7A5)as a direct target for miR-3’*** reduction of LAT1 protein levels by miR-3’28-3p did not alter homeostasis of amino acids within OS cells,suppression of GLUT1 led to a significantly lower glucose uptake and decline in intracellular levels of glucose and glycolytic metabolite ***,combination treatment with hBERA/miR-3’28 and cisplatin or doxorubicin exerted a strong synergism in the inhibition of OS cell *** findings support the utility of novel bioengineered RNA molecules and establish an important role of miR-328-3p in the control of nutrient transport and homeostasis behind cancer metabolism.

关键词： Bioengineered RNA MiR-328 LAT1 GLUT1 Chemosensitivity Cancer

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Author correction to ‘Bioengineered miR-124-3p prodrug selectively alters the proteome of human carcinoma cells to control multiple cellular components and lung metastasis in vivo’ [Acta Pharmaceutica Sinica B 11 (2021) 3950e3965]

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Acta Pharmaceutica Sinica B 2023年第8期13卷 3577-3578页

The authors regret that there was one incorrect image used within Fig.4A,143B cells treated with LSA at 24 h time point,which was obtained from vehicle group but mislabelled as LSA in the *** our studies,three biological replicates were included in each treatment group and at least three images were taken for different fields of each *** thus selected a correct representative image from the LSA treated 143B cells at 24 h time point to replace that mislabelled image,and the updated Fig.4 is provided *** authors apologize for any inconvenience caused to the journal and readers,while this correction does not affect any results and *** authors have provided the original data of this figure to Editorial Office,and the corresponding authors or the Editorial Office can be contacted for original data access.

关键词： journal correction replace

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Tumor cell membrane remodeling with universal ligand for CAR-T cells to inhibit solid tumors

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Science China Chemistry 2023年第11期66卷 3223-3231页

作者： Jin-Xuan Fan Zhong-Bao Hu Hui-yun Gu Wen-Qiang Qu Wen Li Bai-Wen Qi ai-xi yu xian-Zheng Zhang Key Laboratory of Biomedical Polymers of Ministry of Education Department of ChemistryWuhan UniversityWuhan 430072China Department of Orthopedic Trauma and Microsurgery ZhongNan Hospital of Wuhan UniversityWuhan 430071China

Heterogeneity is a major obstacle to the success of CAR-T therapy in treating solid tumors. The complex tumor micro-environment and varying phenotypes of tumor cells might result in antigen escape, drug resistance, and tumor recurrence. To address this issue, we proposed to use lipid-modified fluorescein isothiocyanate(Lip-FITC) as an artificial ligand to normalize the phenotypes of solid tumor cells. In murine cutaneous melanoma and colon adenocarcinoma that prefer to utilize exogenous long-chain fatty acids, we observed much more uptake of Lip-FITC and significantly increased FITC fluorescence on tumor cell membranes than normal cells. This specific exogenous labeling with FITC enhanced the recognition and selectivity of CART cells in solid tumors, bypassing the limitations derived from antigen expression differences in adoptive cell therapies. Lipid metabolism analysis and in vitro experiments demonstrated the sufficient uptake of long-chain fatty acid(LCFAs)-modified Lip-FITC by solid tumor cells, as well as satisfactory ligand assembly on cell membranes. In solid tumor model, the treatment induced the recognition and initiation of CART cells and successfully suppressed tumor progression through T-cell immunity.

关键词： cell membrane CAR-T therapy immune response tumor heterogeneity long-chain fatty acid

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Mechanistic insight into the synergy between nickel single atoms and nanoparticles on N-doped carbon for electroreduction of CO2

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Journal of Energy Chemistry 2025年第1期100卷 327-336页

作者： Mingdong Sun Wenwen Guan Cailing Chen Chao Wu xiaoling Liu Biao Meng Tao Chen yu Han Jun Wang Shibo xi yu Zhou State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical Engineering Nanjing Tech University Advanced Membranes and Porous Materials Center Physical Sciences and Engineering Division King Abdullah University of Science and Technology Institute of Sustainability for Chemicals Energy and Environment A*STAR (Agency for Science Technology and Research) College of Materials Science and Engineering Sichuan University

The synergy of single atoms （SAs） and nanoparticles （NPs） has demonstrated great potential in promoting the electrocatalytic carbon dioxide reduction reaction （CO2RR）;however,the rationalization of the SAs/NPs proportion remains one challenge for the catalyst ***,a Ni2+-loaded porous poly（ionic liquids）(PIL) precursor synthesized through the free radical self-polymerization of the ionic liquid monomer,1-allyl-3-vinylimidazolium chloride,was pyrolyzed to prepare the Ni,N co-doped carbon materials,in which the proportion of Ni SAs and NPs could be facilely modulated by controlling the annealing *** catalyst Ni-NC-1000 with a moderate proportion of Ni SAs and NPs exhibited high efficiency in the electrocatalytic conversion of CO2into *** Ni K-edge X-ray absorption near-edge structure （XANES） spectra and theoretical calculations were conducted to gain insight into the synergy of Ni SAs and *** charge transfer from Ni NPs to the surrounding carbon layer and then to the Ni SAs resulted in the electron-enriched Ni SAs active *** the electroreduction of CO2,the coexistence of Ni SAs and NPs strengthened the CO2activation and the affinity towards the key intermediate of*COOH,lowering the free energy for the potential-determining*CO2→*COOH step,and therefore promoted the catalysis efficiency.

关键词： Single atom catalyst Synergy effect Carbon catalyst Electrocatalysis

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FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression

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Neural Regeneration Research 2025年第7期20卷 2068-2083页

作者： Jing Yao yuan Li xi Liu Wenping Liang yu Li Liyong Wu Zhe Wang Weihong Song The National Clinical Research Center for Geriatric Disease Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Pediatrics The Second Affiliated Hospital and Yuying Children’s HospitalKey Laboratory of Alzheimer’s Disease of Zhejiang ProvinceInstitute of AgingWenzhou Medical UniversityWenzhouZhejiang ProvinceChina Oujiang Laboratory(Zhejiang Laboratory for Regenerative Medicine Visionand Brain Health)WenzhouZhejiang ProvinceChina

Alzheimer's disease is characterized by deposition of amyloid-β,which forms extracellular neuritic plaques,and accumulation of hyperphosphorylated tau,which aggregates to form intraneuronal neurofibrillary tangles,in the *** NLRP3 inflammasome may play a role in the transition from amyloid-βdeposition to tau phosphorylation and *** NLRP3 is primarily found in brain microglia,and tau is predominantly located in neurons,it has been suggested that NLRP3 expressed by microglia indirectly triggers tau phosphorylation by upregulating the expression of pro-inflammatory ***,we found that neurons also express NLRP3 in vitro and in vivo,and that neuronal NLRP3 regulates tau *** biochemical methods,we mapped the minimal NLRP3 promoter and identified FUBP3 as a transcription factor regulating NLRP3 expression in *** primary neurons and the neuroblastoma cell line Neuro2A,FUBP3 is required for endogenous NLRP3 expression and tau phosphorylation only when amyloid-βis *** the brains of aged wild-type mice and a mouse model of Alzheimer's disease,FUBP3 expression was markedly increased in cortical *** analysis suggested that FUBP3 plays a role in neuron-mediated immune *** also found that FUBP3 trimmed the 5′end of DNA fragments that it bound,implying that FUBP3 functions in stress-induced *** findings suggest that neuronal NLRP3 may be more directly involved in the amyloid-β-to–phospho-tau transition than microglial NLRP3,and that amyloid-βfundamentally alters the regulatory mechanism of NLRP3 expression in *** that FUBP3 was only expressed at low levels in young wild-type mice and was strongly upregulated in the brains of aged mice and Alzheimer's disease mice,FUBP3 could be a safe therapeutic target for preventing Alzheimer's disease progression.

关键词： 5′end trimming Alzheimer's disease amyloid-beta amyloid-β-dependent transcription FUBP3 inflammasome inflammation neuron NLRP3 tau transcription factor

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Expert consensus on management principles of orthopedic emergency in the epidemic of coronavirus disease 2019

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Chinese Medical Journal 2020年第9期133卷 1096-1098页

作者： Pei-Fu Tang Zhi-Yong Hou xin-Bao Wu Chang-Qing Zhang Jun-Wen Wang xin xing Zeng-Wu Shao ai-xi yu Gang Wang Bin Chen Ping Zhang Yan-Jun Hu Bo-Wei Wang xiao-Dong Guo xin Tang Dong-Sheng Zhou Fan Liu ai-Mi Chen Kun Zhang Kai-Nan Li Yan-Bin Zhu 无 Department of Orthopedics Department of OrthopedicsThe First Medical CenterChinese PLA General HospitalBeijing 100853China Emergency Center of Trauma Third Hospital of Hebei Medical UniversityShijiazhuangHebei 050051China Department of Traumatology and Orthopedics Beijing Jishuitan HospitalBeijing 100035China Department of Orthopedics The Sixth People’s Hospital Affiliated to Shanghai Jiaotong UniversityShanghai 200233China Department of Orthopedics Wuhan Fourth HospitalWuhanHubei 430034China Department of Orthopedics Union Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhanHubei 430022China Department of Trauma Microsurgery Zhongnan Hospital of Wuhan UniversityWuhanHubei 430071China Department of Orthopedics and Traumatology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515China Department of Orthopedics The First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning 116011China Department of Orthopedics Shandong Provincial HospitalJinanShandong 25002China Department of Orthopedics Affiliated Hospital of Nantong UniversityNantongJiangsu 226001China Department of Orthopedics Changzheng Hospital Affiliated to Naval Military Medical UniversityShanghai 200003China Department of Orthopedics Honghui Hospital Affiliated to Medical College of Xi’an Jiaotong UniversityXi’an Shaanxi 710054China Department of Orthopedics Affiliated Hospital of Chengdu UniversityChengduSichuan 610081China Orthopedic Trauma Branch of Chinese Orthopedic Association External Fixation and Limb Reconstruction Branch of Chinese Orthopedic Association Trauma Expert Committee of Chinese Association of Orthopedic Surgeons

Since December 2019,many patients in Wuhan sustained pneumonia with unknown causes.[1,2]A part of severe patients developed acute respiratory distress syndrome or septic shock,and even *** January 7,2020,Chinese researchers have for the first time detected a new *** January 20,2020,the National Health Commission issued Announcement No.1 of 2020,which included the new coronavirus pneumonia as a Class B infectious disease,and conducted epidemic prevention and control according to the Class A infectious *** new kind of pneumonia has been named coronavirus disease 2019(COVID-19).The sixth edition of"Diagnosis and Treatment Scheme for New Coronavirus Infected Pneumonia"points out that the main transmission routes include respiratory droplets and close contact *** is a possibility of aerosol transmission in a relatively closed environment with prolonged exposure to high concentrations of *** coronavirus pneumonia has a certain incubation period.

关键词： prevention sustained aerosol

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De novo synthesis of atropisomeric benzofurans via Cu/SPDO complex catalyzed asymmetric formal [3 + 2] annulation

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Science China Chemistry 2025年第1期 174-180页

作者： Fan-xiao Meng xing-yu Wang Ka Lu Ju-Song Yang Fu-Min Zhang ai-Jun Ma Bao Du Yong-Qiang Tu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University School of Chemistry and Chemical Engineering Shanghai Jiao Tong University School of Biotechnology and Health Sciences Wuyi University

Herein, the de novo synthesis of atropisomeric benzofurans through the Cu/spirocyclic-pyrrolidine-oxazoline(SPDO) complex catalyze asymmetric formal [3 + 2] cycloaddition between quinones and ynamides is explored, producing a range of structurally diverse axially chiral benzofurans in satisfactory yields with good to excellent enantioselectivities. Furthermore, density functional theory calculations were applied to explain the possible reaction mechanism.

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Recent progress on photoactive nonprecious transition-metal complexes

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Science China Chemistry 2025年第1期 46-95页

作者： Zhong-Liang Gong Hong-Jie Zhang Yan Cheng Jianxun Liu yuhan ai Yaqin Li Zhihui Feng Qiong Zhang Shaolong Gong Yong Chen Chang-Jiang Yao yuan-yuan Zhu Liang-Jin Xu yu-Wu Zhong CAS Key Laboratory of Photochemistry Institute of Chemistry Chinese Academy of Sciences State Key Laboratory of Structural Cemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences School of Chemistry and Chemical Engineering Anhui Key Laboratory of Advanced Catalytic Materials and Reaction EngineeringHefei University of Technology State Key Laboratory of Explosion Science and Safety Protection School of Mechatronical Engineering Beijing Institute of Technology College of Chemistry and Molecular Sciences Hubei Key Lab on Organic and Polymeric Optoelectronic Materials Wuhan University School of Chemistry and Chemical Engineering Anhui University Key Laboratory of Photochemical Conversion and Optoelectronic Materials & CAS-HKU Joint Laboratory on New Materials Technical Institute of Physics and Chemistry Chinese Academy of Sciences

Photoactive complexes of nonprecious transition metals, mainly including those in the first-row and partially the second-row of the Periodic table of elements, have received increasing attention in view of their low cost and long-term sustainability. They are recognized as promising alternatives to noble transition metal complex congeners that have been extensively studied in optoelectronic devices, artificial photosynthesis, photocatalysis, biodiagnostics, and therapeutics, etc. This review is devoted to a comprehensive summary on the classical and recent advances on photoactive nonprecious transition metal complexes, including photoactive Zr, V, Cr, Mo, and W complexes, Mn complexes and hybrids, Fe, Co, Ni, and Cu complexes, and Zn and Cd complexes and hybrids. A particular focus is given on the molecular design, modulation of photophysical and photochemical properties, and applications of the representative and lately-developed nonprecious metal complexes. In addition, a perspective on the future development in this field is provided at the end of this review.

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