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Nitric oxide activation of a potassium channel (BK_(Ca)) in feline lower esophageal sphincter

World Journal of Gastroenterology 2010年第46期16卷 5852-5860页

作者： Marie-Claude L'Heureux ahmad muinuddin Herbert Y Gaisano Nicholas E Diamant Department of Physiology University of Toronto ahmad muinuddin Nicholas E DiamantToronto Western Research InstituteUniversity Health Network Department of Medicine University of Toronto

AIM:To assess the effect of nitric oxide (NO) on the large conductance potassium channel (BKCa) in isolated circular (CM) and sling (SM) muscle cells and muscle strips from the cat lower esophageal sphincter (LES) to determine its regulation of resting tone and ***:Freshly enzymatically-digested and isolated circular smooth muscle cells were prepared from each LES *** study outward K + currents,the perforated patch clamp technique was *** assess LES resting tone and relaxation,muscle strips were mounted in perfused organ ***:(1) Electrophysiological recordings from isolated cells:(a) CM was more depolarized than SM (-39.7 ± 0.8mV vs-48.1 ± 1.6 mV,P 0.05);(b) The NO donor sodium nitroprusside (SNP) increased outward currents only in CM (25.9 ± 1.9 to 46.7 ± 4.2 pA/pF,P 0.05);(c) SNP added in the presence of the BK Ca antagonist iberiotoxin (IbTX) produced no increase in the outward current in CM (17.0 ± 2.8 vs 13.7 ± 2.2,P > 0.05);and (d) L-NNA caused a small insignificant inhibition of outward K + currents in both muscles;and (2) Muscle strip studies:(a) Blockade of the nerves with tetrodotoxin (TTX),or BK Ca with IbTX had no significant effect on resting tone of either muscle;and (b) SNP reduced tone in both muscles,and was unaffected by the presence of TTX or ***:Exogenous NO activates BK Ca only in CM of the ***,as opposed to other species,exogenous NO-induced relaxation is predominantly by a non-BK Ca mechanism,and endogenous NO has minimal effect on resting tone.

关键词： Circular smooth muscle Feline K+ channel Lower esophageal sphincter Nitric oxide Sling Tone

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