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Neuroinflammation in glaucoma：soluble tumor necrosis factor alpha and the connection with excitotoxic damage

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Neural Regeneration Research 2016年第3期11卷 424-426页

作者： Jorge L.Cueva Vargas adriana di polo Department of Neuroscience and Centre de recherche du Centrehospitalier de l'Universite de Montreal (CRCHUM) University of Montreal Montreal Quebec H3R2T6 Canada

Inflammation is a complex and highly regulated response that occurs early after infection or *** process is initiated by cells of the immune system to re-establish tissue *** the injury is persistent,however,chronic i... 详细信息

关键词： glaucoma homeostasis inflammation initiated persistent RGCs permeability synaptic glutamate excitatory

*** stress in glaucoma engages early activation of the energy biosensor adenosine monophosphate-activated protein kinase leading to neuronal dysfunction

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Annals of Eye Science 2018年第1期 421-421页

作者： Nicolas Belforte Jorge L.Cueva Vargas adriana di polo Department of Neuroscience University of Montreal Hospital Research Center(CR-CHUM)MontrealQuebecCanada

Background:Metabolic stress has been proposed to contribute to neuronal damage in glaucoma,but the mechanism driving this response is not *** adenosine monophosphate-activated protein kinase(AMPK)is a master regulator of energy homeostasis that becomes active at the onset of energy *** is a potent inhibitor of the mammalian target of rapamycin complex 1(mTORC1),which we showed is essential for the maintenance of retinal ganglion cell(RGC)dendrites,synapses,and ***,we tested the hypothesis that AMPK is an early mediator of metabolic stress in ***:Unilateral elevation of intraocular pressure was induced by injection of magnetic microbeads into the anterior chamber of mice expressing yellow fluorescent protein in *** of AMPK was achieved by administration of siRNA or compound *** dendritic trees were 3D-reconstructed and analyzed with Imaris(Bitplane),and survival was assessed by counting Brn3a or RBPMS-labeled soma and axons in the optic *** function was examined by quantification of anterograde axonal transport after intraocular administration of cholera toxinβ-*** from glaucoma patients were analyzed for expression of active ***:Ocular hypertension triggered rapid upregulation of AMPK activity in RGCs concomitant with loss of mTORC1 *** inhibition with compound C or siRNA effectively restored mTORC1 activity and promoted an increase in total dendritic length,surface and complexity relative to control *** of AMPK activity led to robust RGC soma and axon *** example,95%of RGCs(2,983±258 RGCs/mm2,mean±S.E.M.)survived with compound C compared to 77%in vehicle-treated eyes(2,430±233 RGCs/mm2)(ANOVA,P<0.001)at three weeks after glaucoma induction(n=8-10/group).Importantly,blockade of AMPK activity effectively restored anterograde axonal ***,RGC-specific upregulation of AMPK activity was detected in human glaucomatous retinas relative to age-ma

关键词： Metabolic stress retinal ganglion cell(RGC) adenosine monophosphate-activated protein kinase(AMPK) glaucoma

*** on microvessels lead to vascular dysfunction during retinal ischemia

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Annals of Eye Science 2018年第1期 448-448页

作者： Deborah Villafranca-Baughman Luis Alarcón-Martínez adriana di polo Department of Neuroscience University of Montreal Hospital Research Center(CR-CHUM)MontrealQuebecCanada

Background:Pericytes are contractile cells that wrap along the walls of *** the brain,pericytes play a crucial role in the regulation of capillary diameter and vascular blood flow in response to metabolic *** ischemia,it has been suggested that pericytes may constrict capillaries,and that pericytes remain constricted after reperfusion thus resulting in impaired blood ***:Here,we used a mouse model of retinal ischemia based on ligation of the central retinal artery to characterize the role of pericytes on capillary *** was induced in transgenic mice carrying the NG2 promoter driving red fluorescent protein expression to selectively visualize pericytes(line NG2:DsRed).Changes in retinal capillary diameter at 1 hr after ischemia were measured ex vivo in whole-mounted retinas from ischemic and control eyes(n=4-6/group)using a stereological *** and pericytes were three-dimensionally reconstructed using IMARIS(Bitplane).Furthermore,we used a novel and minimally invasive two-photon microscopy approach that allowed live imaging of microvasculature changes in the ***:Our data show a generalized reduction in capillary diameter in ischemic retinas relative to sham-operated controls in all vascular plexus(ischemia:4.7±0.2μm,control:5.2±0.2µm,student’s t-test,Pdiameter reductions observed in controls(ischemia:1,038±277 constrictions at pericyte locations,control:60±36 constrictions at pericyte locations,student’s t-test,P<0.01).Live imaging using two-photon microscopy confirmed robust capillary constriction at the level of pericytes on retinal capillaries during ischemia(n=6-8/group).Conclusions:Collectively,our data demonstrate that ischemia promotes rapid pericyte constriction on retinal capillaries causi

关键词： Ischemia pericyte blood flow regulation in vivo two-photon microscopy

*** imaging of retinal pericytes:evidence for early calcium uptake,capillary constriction and vascular dysregulation in ocular hypertension glaucoma

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Annals of Eye Science 2018年第1期 417-417页

作者： Luis Alarcon-Martinez Jorge L.Cueva Vargas Nicolas Belforte Deborah Villafranca-Baughman adriana di polo Department of Neuroscience University of Montreal Hospital Research Center(CR-CHUM)MontrealQuebecCanada

Background:Pericytes are contractile cells that wrap along the walls of *** the brain,pericytes play a crucial role in the regulation of capillary diameter and vascular blood flow in response to metabolic *** contribution of pericytes to microvascular deficits in glaucoma is currently *** address this,we used two-photon excitation microscopy for longitudinal monitoring of retinal pericytes and capillaries in a mouse glaucoma ***:Ocular hypertension was induced by injection of magnetic microbeads into the anterior chamber of albino mice expressing red fluorescent protein selectively in pericytes(NG2-DsRed).Minimally invasive,multiphoton imaging through the sclera of live NG2-DsRed mice was used to visualize pericytes and capillary diameter at one,two and three weeks after glaucoma *** vivo fluctuations in pericyte intracellular calcium were monitored with the calcium indicator *** vivo stereological analysis of retinal tissue prior to and after injection of microbeads was used to confirm our in vivo ***:Live two-photon imaging of NG2-DsRed retinas demonstrated that ocular hypertension induced progressive accumulation of intracellular calcium in *** uptake correlated directly with the narrowing of capillaries in the superficial,inner,and outer vascular plexuses(capillary diameter:naïve control=4.7±0.1μm,glaucoma=4.0±0.1μm,n=5-6 mice/group,Student’s t-test Pdistribution analysis showed a substantial increase in the number of small-diameter capillaries(≤3μm)and a decrease in larger-diameter microvessels(≥5-9μm)at three weeks after induction of ocular hypertension(n=5-6 mice/group,Student’s t-test Pdinal changes in retinal pericytes and capillaries in live animals at glaucoma onset and ***,ocular hypertension triggers rapid int

关键词： Glaucoma pericytes capillaries in vivo two-photon microscopy

*** hypertension promotes early mitochondrial fragmentation in retinal endothelial cells in a mouse model of glaucoma

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Annals of Eye Science 2018年第1期 424-424页

作者： Jorge L.Cueva Vargas Yoko Ito Khalil Miloudi Ariel M.Wilson Christine Vande Velde Przemyslaw Sapieha adriana di polo Department of Neuroscience University of Montreal Hospital Research Center(CR-CHUM)MontrealQuebecCanada Department of Biochemistry Maisonneuve-Rosemont Hospital Research CentreUniversity of MontrealMontrealQuebecCanada Department of Ophthalmology Maisonneuve-Rosemont Hospital Research CentreUniversity of MontrealMontrealQuebecCanada Department of Neuroscience McGill UniversityMontrealQuebecCanada

Background:Retinal endothelial cells are very active and contribute to the integrity of the neurovascular *** dysfunction has been proposed to contribute to the pathogenesis of ***,we evaluated the hypothesis that ocular hypertension triggers mitochondrial alterations in endothelial cells impairing the integrity of the blood retinal barrier(BRB).Methods:Ocular hypertension was induced by injection of magnetic microbeads into the anterior chamber of EndoMito-EGFP mice,a strain expressing green fluorescent protein selectively in the mitochondria of endothelial *** density,mitochondrial volume,and the number of mitochondrial components were quantified in 3D-reconstructed images from whole-mounted retinas using Imaris ***-related protein(DRP-1),mitofusin-2(MFN-2)and optic atrophy-1(OPA-1)expression were assessed by western blot analysis of enriched endothelial *** structure was evaluated by transmission electron microscopy(TEM)and oxygen consumption rate was monitored by Seahorse *** integrity of the BRB was evaluated by quantifying Evans blue ***:Our data demonstrate that two and three weeks after ocular hypertension induction,the total mitochondria volume in endothelial cells decreased from 0.140±0.002µm3 from non-injured retinas to 0.108±0.005 and 0.093±0.007µm3,respectively in glaucomatous eyes(mean±S.E.M,ANOVA,Pdistribution showed a substantial increase of smaller mitochondria complexes(diti

关键词： Glaucoma endothelial cells mitochondria vascular leakage

Controlling Properties of HSLA Line Pipe Steels for Oil and Gas Applications

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Journal of Iron and Steel Research International 2011年第S1期18卷 701-706页

作者： Mukhopadhyay Ananya polo Andrea Danieli Automation SpA via Stringher 433042 ButtrioUdineItaly

DANIELI has recently implemented its Coil Quality Estimator (DANIELI-CQETM) system to the Hot Strip Mill of United Metallurgical Company (OMK) at Vyksa,*** system is developed for the purpose of real time assessment and control of mechanical properties for hot rolled *** properties such as strength,toughness,ductility and hardness are predicted over the entire length of a strip while it is *** property estimation is based on the final microstructure as predicted from a group of interconnected physically based metallurgical models,supplemented by Artificial Neural *** CQE system is used for prediction and control of properties of HSLA line pipe grades steel and other *** system performance,is judged by accuracy and reliability of prediction,has been compared with the physical material testing data from the *** results are found to be *** is found useful for generation of test certificate of coil,quality assurance,process control,product development,and customer claim *** is used for resource optimization for production,and other operational improvements such as reduction of *** present paper shares the results of CQE performance for prediction of HSLA line pipe grade steels.

关键词： line pipe steels CQE mechanical property ANN process control

Non-human primate pluripotent stem cells for the preclinical testing of regenerative therapies

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Neural Regeneration Research 2022年第9期17卷 1867-1874页

作者： Ignacio Rodríguez-polo Rüdiger Behr Research Platform Degenerative diseases German Primate Center-Leibniz Institute for Primate ResearchGöttingenGermany German Center for Cardiovascular Research(DZHK) Partner Site GöttingenGöttingenGermany

Non-human primates play a key role in the preclinical validation of pluripotent stem cell-based cell replacement *** stem cells used as advanced therapy medical products boost the possibility to regenerate tissues and organs affected by degenerative ***,the methods to derive human induced pluripotent stem cell and embryonic stem cell lines following clinical standards have quickly developed in the last 15 *** the preclinical validation of cell replacement therapies in non-human primates,it is necessary to generate non-human primate pluripotent stem cell with a homologous quality to their human ***,pluripotent stem cell technologies have developed at a slower pace in non-human primates in comparison with human cell *** recent years,however,relevant progress has also been made with non-human primate pluripotent stem *** review provides a systematic overview of the progress and remaining challenges for the generation of non-human primate induced pluripotent stem cells/embryonic stem cells for the preclinical testing and validation of cell replacement *** focus on the critical domains of(1)reprogramming and embryonic stem cell line derivation,(2)cell line maintenance and characterization and,(3)application of non-human primate pluripotent stem cells in the context of selected preclinical studies to treat cardiovascular and neurodegenerative disorders performed in non-human primates.

关键词： embryonic stem cells induced pluripotent stem cells non-human primates pluripotent stem cells preclinical regeneration reprogramming translational research

Synthesis, characterization,thermal behavior,and antifungal activity of La(Ⅲ) complexes with cinnamates and 4-methoxyphenylacetate

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Journal of Rare Earths 2013年第11期31卷 1106-1113页

作者： Alberto Aragón-Muriel Dorian polo-Cerón Departamento de Química Universidad del Valle Calle 13 No 100-00 CaliColombia 76001000

In this study a series of trivalent lanthanum complexes with 4-（R）-cinnamate （4-Rcinn, R=H（1）, MeO（2）, Cl（3）） and 4-methoxyphenylacetate ligands （4） were prepared and their antifungal activity against Candida albicans, Aspergillus niger and Trichophyton mentagrophytes were examined. Compounds 1-4 were synthesized by a metathesis reaction and fully characterized by elemental analysis, IR, 1H and 13C NMR spectroscopy, fluorescence spectra, thermogravimetry （TG）, derivative thermogravimetry （DTG）, differential scanning calorimetry （DSC） and X-ray diffraction powder patterns. In emission studies, it was observed that lu-minescence intensity was enhanced in the presence of lanthanide ion. The results of X-ray diffraction patterns indicated that all com-plexes studied exhibited crystalline structure. Thermal behavior by TG, DTG, and DSC studies permitted to estimating the hydration degree of the compounds and showed the formation of decomposition products like lanthanum oxide. Determined by antifungal stud-ies, lanthanum complexes 1-4 demonstrated antifungal activity toward all pathogenic fungal strains tested. Compounds 2 and 4 showed significant growth inhibition for A. niger and C. albicans, respectively.

关键词： antifungal activity La(III) complexes cinnamates 4-methoxyphenylacetate infrared spectra excitation emission TG-DTG-DSC Candida albicans Aspergillus niger Trichophyton mentagrophytes rare earths

The biological stress of early weaned piglets

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Journal of Animal Science and Biotechnology 2013年第2期4卷 124-127页

作者： Joy M Campbell Joe D Crenshaw Javier polo APC Inc. 2425 SE Oak Tree CourtAnkenyIA USA

Pigs experience biological stress such as physiological, environmental, and social challenges when weaned from the sow. The process of weaning is one of the most stressful events in the pig＇s life that can contribute to intestinal and immune system dysfunctions that result in reduced pig health, growth, and feed intake, particularly during the first week after weaning. Technological improvements in housing, nutrition, health, and management have been used to minimize some of the adverse effects of weaning stress, but a greater understanding of the biological impact of stress is needed to improve strategies to overcome weaning stress. The focus of this review paper is to briefly describe how the biological stress associated with weaning impacts intestinal morphology, structure, physiology, and intestinal immune responses that can impact subsequent production efficiencies such as growth, intake, morbidity, and mortality.

关键词： Biological stress Intestine Pig Weaning