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Integrative phosphoproteomics defines two biologically distinct groups of KMT2A rearranged acute myeloid leukaemia with different drug response phenotypes

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Signal Transduction and Targeted Therapy 2023年第3期8卷 1318-1331页

作者： Pedro Casado Ana Rio-Machin Juho JMiettinen Findlay Bewicke-Copley Kevin Rouault-Pierre Szilvia Krizsan Alun Parsons Vinothini Rajeeve Farideh Miraki-Moud david CTaussig Csaba Bödör John Gribben Caroline Heckman Jude Fitzgibbon Pedro R.Cutillas Cell Signalling and Proteomics Group Centre for Genomics and Computational BiologyBarts Cancer InstituteQueen Mary University of LondonLondon EC1M6BQUK Centre for Genomics and Computational Biology Barts Cancer InstituteQueen Mary University of LondonLondon EC1M6BQUK Institute for Molecular Medicine Finland-FIMM HiLIFE-Helsinki Institute of Life ScienceiCAN Digital Precision Cancer Medicine FlagshipUniversity of HelsinkiHelsinkiFinland Centre for Haemato-Oncology Barts Cancer InstituteQueen Mary University of LondonLondon EC1M6BQUK HCEMM-SU Molecular Oncohematology Research Group 1st Department of Pathology and Experimental Cancer ResearchSemmelweis University BudapestBudapestHungary Cancer Research UK Cancer Therapeutics Unit Division of Cancer TherapeuticsThe Institute of Cancer ResearchSuttonUK a The Alan Turing Institute The British Library2QR96 Euston RdLondon NW12DBUK

Acute myeloid leukaemia(AML)patients harbouring certain chromosome abnormalities have particularly adverse *** these patients,targeted therapies have not yet made a significant clinical *** understand the molecular landscape of poor prognosis AML we profiled 74 patients from two different centres(in UK and Finland)at the proteomic,phosphoproteomic and drug response phenotypic *** data were complemented with transcriptomics analysis for 39 *** integration highlighted a phosphoproteomics signature that define two biologically distinct groups of KMT2A rearranged leukaemia,which we term MLLGA and *** presented increased dOT1L phosphorylation,HOXA gene expression,CdK1 activity and phosphorylation of proteins involved in RNA metabolism,replication and dNA damage when compared to MLLGB and no KMT2A rearranged *** was particularly sensitive to 15 compounds including genotoxic drugs and inhibitors of mitotic kinases and inosine-5-monosphosphate dehydrogenase(IMPdH)relative to other ***-risk KMT2A-MLLT3 cases were mainly represented in a third group closer to MLLGA than to *** expression of IMPdH2 and multiple nucleolar proteins was higher in MLLGA and correlated with the response to IMPdH inhibition in KMT2A rearranged leukaemia,suggesting a role of the nucleolar activity in sensitivity to *** summary,our multilayer molecular profiling of AML with poor prognosis and KMT2A-MLLT3 karyotypes identified a phosphoproteomics signature that defines two biologically and phenotypically distinct groups of KMT2A rearranged *** data provide a rationale for the potential development of specific therapies for AML patients characterised by the MLLGA phosphoproteomics signature identified in this study.

关键词： metabolism signature biologically

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Inhibition of N-Myc down regulated gene 1 in in vitro cultured human glioblastoma cells

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World Journal of Clinical Oncology 2012年第7期3卷 104-110页

作者： Harun M Said Buelent Polat Susanne Stein Mathias Guckenberger Carsten Hagemann adrian Staab Astrid Katzer Jelena Anacker Michael Flentje dirk Vordermark department of Radiation Oncology University of Wuerzburg 97080 Würzburg Germany department of Hematology and Oncology Johannes Gutenberg University Ⅲ Medical School 55101 Mainz Germany department of Neurosurgery Tumorbiology Laboratory University of Wuerzburg 97080 Würzburg Germany department of Radiation Oncology Paul Scherer Institute CH-5100 Villingen Switzerland department of Gynaecology and Obstetrics University of Wuerzburg 97080 Würzburg Germany department of Radiation Oncology University-Halle-Wittenberg 06110 Halle Germany

AIM: To study short ds RNA oligonucleotides(si RNA)as a potent tool for artificially modulating gene expression of N-Myc down regulated gene 1(NdRG1) gene induced under different physiological conditions(Normoxia and hypoxia) modulating NdRG1 transcription, m RNA stability and translation. METHOdS: A cell line established from a patient with glioblastoma multiforme. Plasmid dNA for transfections was prepared with the Endofree Plasmid Maxi kit. From plates containing 5 × 107 cells, nuclear extracts were prepared according to previous protocols. The p SUPERNdRG1 vectors were designed, two sequences were selected from the human NdRG1 c dNA(5'-GCATTATTGGCATGGGAAC-3' and 5'-ATGCAGAGTAACGTGGAAG-3'. reverse transcription polymerase chain reaction was performed using primers designed using published information on β-actin and hypoxia-inducible factor(HIF)-1α m RNA sequences in Gen Bank. NdRG1 m RNA and protein level expression results under different conditions of hypoxia or reoxygenation were compared to aerobic control conditions using the Mann-Whitney U test. Reoxygenation values were also compared to the NdRG1 levels after 24 h of hypoxia(P dered significant).RESULTS: si RNA- and iodoacetate(IAA)-mediated downregulation of NdRG1 m RNA and protein expression in vitro in human glioblastoma cell lines showed a nearly complete inhibition of NdRG1 expression when compared to the results obtained due to the inhibitory role of glycolysis inhibitor IAA. Hypoxia responsive elements bound by nuclear HIF-1 in human glioblastoma cells in vitro under different oxygenation conditions and the clearly enhanced binding of nuclear extracts from glioblastoma cell samples exposed to extreme hypoxic conditions confirmed the HIF-1 Western blotting results. CONCLUSION: NdRG1 represents an additional diagnostic marker for brain tumor detection, due to the role of hypoxia in regulating this gene, and it canrepresent a potential target for tumor treatment in human glioblastoma. T

关键词： N-Myc down regulated gene 1 Short dsRNA oligonucleotides Human cancer diseases Brain cancer Radiotherapy

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超声定位与体表标志定位在儿童髂腹股沟-髂腹下神经阻滞中的比较：罗哌卡因血浆浓度测定的临床指导意义

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麻醉与镇痛 2012年第3期8卷 33-37页

作者： Marion Weintraud, Md Marit Lundblad, Md Stephan C. Kettner, Md Harald Willschke, Md Stephan Kapral, Md Per-Arne Loennqvist, Md Karl Koppatz Klaus Turnheim, Md adrian Boesenberg, Md Peter Marhofer, Md 周洁(译) 李士通(校) department of Anesthesia and Intensive Care Medicine Medical University of Vienna Vienna Austria epartment of Anesthesia and Intensive Care Karolinska Hospital/Astrid Lindgren Hospital Stockholm Sweden department of Pharmacology Medical University of Vienna Vienna Austria Red Cross Children Hospital Cape Town South Africa department of Anesthesia University Cape Town Red Cross Children Hospital Cape Town South Africa 不详

背景儿童髂腹股沟-髂腹下神经阻滞常导致血浆局部麻醉药浓度过高。超声引导能为注射局麻药提供准确的解剖定位，从而影响局麻药的血浆浓度变化。为此，我们比较了超声定位与体表标志定位下行神经阻滞对罗哌卡因血浆浓度的影响。方法在... 详细信息

背景儿童髂腹股沟-髂腹下神经阻滞常导致血浆局部麻醉药浓度过高。超声引导能为注射局麻药提供准确的解剖定位，从而影响局麻药的血浆浓度变化。为此，我们比较了超声定位与体表标志定位下行神经阻滞对罗哌卡因血浆浓度的影响。方法在全麻诱导下，将66例准备行腹股沟疝修补术的儿童随机分为两组，年龄为8个月-7岁，一组在超声定位下行髂腹股沟-髂腹下神经阻滞（n=35），另一组在体表标志定位下行髂腹股沟-髂腹下神经阻滞（n=31），均使用0．5％的罗哌卡因0．25ml／kg（1．25mg／kg），分别在给药后0、5、10、20、30分钟用高效液相色谱法测罗哌卡因的血浆浓度，观察最大血浆浓度（Cmax），达峰时间（tmax），吸收率常数（ka），在时间0分钟时血浆浓度的上升速度（dC0／d1）和曲线下面积值（AUC）。结果使用超声定位技术阻滞患儿的Cmax（sd）、ka、dC0／dt和AUC的值均高于使用标志定位技术的值，而tmax的值也短于使用标志定位技术的值。[Cmax：1．78（0．62）vs1．23（0．70）μg／ml，P〈0．01；ka：14．4（10．7）vs11．7（11．4）h^-1，P〈0．05；dC0／d1：0．26（0．12）vs0．15（0．03）μg·ml^-1·min^-1，P〈0．01；AUC：42．4（15．9）vs27．2（18．1）μg·30min^-1·ml^-1，P〈0．001；tmax：20．4（8．6）vs25．3（7．6）分钟，P〈0．05）。结论药物代谢动力学数据表明：与体表标志定位相比，在超声定位下行髂腹股沟-髂腹下神经阻滞局麻药的最大血浆浓度更高，药物吸收也更快。因此，在超声定位下行髂腹股沟-髂腹下神经阻滞时需要考虑减少局麻药的用量。

关键词：神经阻滞血浆浓度髂腹股沟超声定位罗哌卡因体表标志髂腹下临床指导

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A history of high-power laser research and development in the United Kingdom

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High Power Laser Science and Engineering 2021年第2期9卷 41-126页

作者： Colin N.danson Malcolm White John R.M.Barr Thomas Bett Peter Blyth david Bowley Ceri Brenner Robert J.Collins Neal Croxford A.E.Bucker dangor Laurence devereux Peter E.dyer Anthony dymoke-Bradshaw Christopher B.Edwards Paul Ewart Allister I.Ferguson John M.Girkin denis R.Hall david C.Hanna Wayne Harris david I.Hillier Christopher J.Hooker Simon M.Hooker Nicholas Hopps Janet Hull david Hunt dino A.Jaroszynski Mark Kempenaars Helmut Kessler Sir Peter L.Knight Steve Knight adrian Knowles Ciaran L.S.Lewis Ken S.Lipton Abby Littlechild John Littlechild Peter Maggs Graeme P.A.Malcolm OBE Stuart P.d.Mangles William Martin Paul McKenna Richard O.Moore Clive Morrison Zulfikar Najmudin david Neely Geoff H.C.New Michael J.Norman Ted Paine Anthony W.Parker Rory R.Penman Geoff J.Pert Chris Pietraszewski Andrew Randewich Nadeem H.Rizvi Nigel Seddon MBE Zheng-Ming Sheng david Slater Roland A.Smith Christopher Spindloe Roy Taylor Gary Thomas John W.G.Tisch Justin S.Wark Colin Webb S.Mark Wiggins dave Willford Trevor Winstone AWE Aldermaston ReadingUK Oxford Centre for High Energy density Science Department of PhysicsClarendon LaboratoryUniversity of OxfordOxfordUK Centre for Intertial Fusion Studies Blackett LaboratoryImperial College LondonLondonUK Formerly of Electrox LetchworthUK Formerly of Central Laser Facility Rutherford LaboratoryDidcotUK Formerly of Ferranti defence Systems LincolnUK Leonardo EdinburghUK Retired AWEReadingUK Formerly of AWE ReadingUK Formerly of Laser Lines Ltd BanburyUK Formerly of Optilas Ltd Milton KeynesUK Retired Pro-Lite TechnologyCranfieldUK Specialised Imaging Ltd PitstoneUK Central Laser Facility STFC Rutherford Appleton LaboratoryDidcotUK Magna-Power Electronics Ltd ReadingUK deBe Lasers Needham Laser TechnologiesWhitchurchUK Blackett Laboratory Imperial College LondonLondonUK SPIE Europe CardiffUK Professor Emeritus(Physics) University of HullKingston upon HullUK Kentech Instruments Ltd WallingfordUK department of Physics Clarendon LaboratoryUniversity of OxfordOxfordUK Professor Emeritus Department of PhysicsUniversity of StrathclydeGlasgowUK department of Physics Durham UniversityDurhamUK Professor Emeritus(Photonics) Heriot-Watt UniversityEdinburghUK Professor Emeritus University of SouthamptonSouthamptonUK Optical Surfaces Ltd KenleyUK JEH Lasers Ltd RugbyUK Scottish Universities Physics Alliance Department of PhysicsUniversity of StrathclydeGlasgowUK ITER Organization Saint-Paul-lez-DuranceFrance Manx Precision Optics Ltd BallasallaUK Laser Lines Ltd BanburyUK Elliot Scientific Ltd MetroTest Scientific GroupHarpendenUK Centre for Plasma Physics Queen’s University BelfastBelfastUK Retired Founderand former MDRofin-Sinar UK LtdHullUK Heraeus Noblelight Ltd CambridgeUK Mirli Books ChelmsfordUK M Squared Lasers Ltd GlasgowUK University of Hertfordshire HatfieldUK E&EO UK Ltd Barton-upon-HumberUK department of Physics University of YorkYorkUK IC Optical Systems Ltd BeckenhamUK Laser Micromachining Ltd St AsaphUK MBdA UK Ltd Brist

The first demonstration of laser action in ruby was made in 1960 by *** of Hughes Research Laboratories,*** laboratories worldwide began the search for lasers using different materials,operating at different *** the UK,academia,industry and the central laboratories took up the challenge from the earliest days to develop these systems for a broad range of *** historical review looks at the contribution the UK has made to the advancement of the technology,the development of systems and components and their exploitation over the last 60 years.

关键词： high-power lasers history United Kingdom

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Comparative study of breast cancer in Mexican and Mexican-American women

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Health 2010年第9期2卷 1040-1048页

作者： Maria Elena Martinez Luis Enrique Gutierrez-Millan Melissa Bondy adrian daneri-Navarro Maria Mercedes Meza-Montenegro Ivan Anduro-Corona Ma Isabel Aramburo-Rubio Luz Maria adriana Balderas-Pena Jose Adelfo Barragan-Ruiz Abenaa Brewster Graciela Caire-Juvera Juan Manuel Castro-Cervantes Mario Alberto Chavez Zamudio Giovanna Cruz Alicia del Toro-Arreola Mary EEdgerton Maria Rosa Flores-Marquez Ramon Antonio Franco-Topete Helga Garcia Susan Andrea Gutierrez-Rubio Karin Hahn Luz Margarita Jimenez-Perez Ian KKomenaka Zoila Areli Lopez Bujanda dihui Lu Gilberto Morgan-Villela James LMurray Jesse NNodora Antonio Oceguera-Villanueva Miguel Angel Ortiz Martinez Laura Perez Michel Antonio Quintero-Ramos Aysegul Sahin Jeong Yun Shim Maureen Stewart Gonzalo Vazquez-Camacho Betsy Wertheim Rachel Zenuk Patricia Thompson Arizona Cancer Center and Mel and Enid Zuckerman College of Public Health University of ArizonaTucsonUSA departamento de Investigaciones Cientificas y Tecnologicas Universidad de SonoraHermosilloMexico University of Texas M.d.Anderson Cancer Center HoustonUSA departamento de Fisiologia Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraMexico Instituto Tecnologico de Sonora Ciudad ObregónMexico Instituto Mexicano del Seguro Social HermosilloMexico Instituto Mexicano del Seguro Social CMNOGuadalajaraJalisco Centro de Investigacion en Alimentacion y desarrollo HermosilloMexico Instituto Mexicano del Seguro Social Ciudad ObregonMexico OPd Hospital Civil de Guadalajara GuadalajaraMexico departamento de Salud Publica Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraMexico Maricopa Medical Center Department of SurgeryPhoenixUSA University of North Carolina Lineberger Cancer CenterChapel HillUSA Arizona Cancer Center and department of Family and Community Medicine University of ArizonaTucsonUSA Instituto Jalisciense de Cancerologia GuadalajaraMexico

Breast cancer is the number one cause of can- cer deaths among Hispanic women in the United States, and in Mexico, it recently became the primary cause of cancer deaths. This malign- nancy represents a poorly understood and un- derstudied disease in Hispanic women. The ELLA Binational Breast Cancer Study was es- tablished in 2006 as a multi-center study to as- sess patterns of breast tumor markers, clinical characteristics, and their risk factors in women of Mexican descent. We describe the design and implementation of the ELLA Study and provide a risk factor comparison between women in the U.S. and those in Mexico based on a sample of 765 patients (364 in the U.S. and 401 in Mexico). Compared to women in Mexico, U.S. women had significantly (p d breastfeeding rates (57.5% vs. 80.5%), higher use of oral contraceptives (60.7% vs. 50.1%) and hormone replacement therapy (23.3% vs. 7.6%), and higher family history of breast cancer (15.7% vs. 9.0%). Re- sults show that differences in breast cancer risk factor patterns exist between Mexico and U.S. women. We provide lessons learned from the conduct of our study. Binational studies are an important step in understanding disease pat- terns and etiology for women in both countries.

关键词： Binational Study Breast Cancer Hispanics Mexico United States

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The enhanced X-ray Timing and Polarimetry mission—eXTP

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Science China(Physics,Mechanics & Astronomy) 2019年第2期62卷 3-27页

作者： ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra de Rosa YongWei dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas dauser Ettore del Monte Sergio di Cosimo Sebastian diebold Victor doroshenko Michal dovciak YuanYuan du Lorenzo ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu Yudong Gu daWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo daniele Macera Piero Malcovati adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang dianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu Wenda Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Key Laboratory for Particle Astrophysics Institute of High Energy PhysicsChinese Academy of Sciences Institut für Astronomie und Astrophysik Eberhard Karls Universität INAF-Istituto di Astrofisica e Planetologia Spaziali INFN-Roma Tor Vergata Via della Ricerca Scientifica 1 department of Engineering Physics and Center for Astrophysics Tsinghua University dTU Building 327DK-2800 Kongens Institute of Space Sciences 08193 Cerdanyola del Valles Institut d’Estudis Espacials de Catalunya Sezione di Pisa Istituto Nazionale di Fisica NucleareLargo Bruno Pontecorvo3 department of Astronomy University of Genevachemin d’Ecogia 16 Osservatorio di Astrofisica e Scienza dello Spazio di Bologna Istituto Nazionale di Astofisica Astronomical Institute Czech Academy of Sciences Max Planck Institute for Extraterrestrial Physics Giessenbachstr.1 CEA Paris-Saclay/IRFU Beijing Institute of Spacecraft System Engineering CAST Osservatorio Astronomico di Brera Istituto Nazionale di Astofisica Space Research Center Polish Academy of Sciences Key Laboratory of Advanced Material Microstructure of Education Ministry of China Institute of Precision Optical EngineeringSchool of Physics Science and EngineeringTongji University department of Physics and Center for Astrophysics Tsinghua University Universita’degli Studi di Udine Via delle Scienze 206 Mullard Space Science Laboratory University College London department of Nuclear and Particle Physics University of Geneva SRON Netherlands Institute for Space Research Politecnico di Milano Sezione di Perugia Istituto Nazionale di Fisica Nucleare dipartimento di Fisica e Chimica Fondazione Bruno Kessler dr.Karl Remeis-Observatory and Erlangen Centre for Astroparticle Physics Universität Erlangen-Nürnberg University of Pavia Department of ElectronicsInformation and Biomedical Engineering and INFN Pavia Sezione di Torino Istituto Nazionale di Fisica NucleareVia Pietro Giuria1 department of Physics and Astronomy University of Leicester Sezione di Trieste Istituto Nazionale di Fisica Nucleare

In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QEd, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave *** paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment.

关键词： X-ray instrumentation X-ray polarimetry X-ray timing space mission:eXTP

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不同方法获得的脂肪来源干细胞再生能力比较：超声辅助吸脂与传统脂肪抽吸对比

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中国美容整形外科杂志 2015年第8期26卷 J0011-J0011页

作者： david Atashroo dominik duscher Zeshaan N. Maan Elizabeth R. Zielins Kevin Paik Alexander Whittam Michelle Lin adrian McArdle Christopher duldulao t Graham G. Walmsley Michael S.Hu Ruth Tevlin derrick C. Wan Geoffrey C. Gurtner Michael T. Longaker 朱利娜高景恒张晨袁继龙不详《中国美容整形外科杂志》编辑部大连大学附属新华医院整形外科辽宁省人民医院整形外科

背景与目的超声辅助吸脂（ultrasound assisted liposuction，UAL）因其获取脂肪容易，且可让创伤和失血最小化，受到整形外科医师的青睐。但是，很少有研究报道这种方法获取的脂肪来源干细胞（adipose derived stromal cells，AdSCs）... 详细信息

背景与目的超声辅助吸脂（ultrasound assisted liposuction，UAL）因其获取脂肪容易，且可让创伤和失血最小化，受到整形外科医师的青睐。但是，很少有研究报道这种方法获取的脂肪来源干细胞（adipose derived stromal cells，AdSCs）的质量。AdSCs在基于细胞的治疗方法中的作用也在急剧增长。因此，本研究比较了传统脂肪抽吸（suctionassistedliposuction，SAL）方法获得的AdSCs与UAL方法获得的AdSCs之间再生能力的差异。方法分别采用SAL和UAL方法，从健康人中获取脂肪并培养AdSCs；采用荧光辅助细胞分选（fluorescent assisted cell sorting，FACS）方法，分析AdSCs的产量。利用MTT比色法分析AdSCs的增殖能力，并在培养基中进行成脂和成骨分化诱导。将基因表达谱与qPCR进行对比。利用细胞染色比较成脂和成骨分化标记物。建立小鼠创伤愈合模型，在水凝胶支架上，分别播种采用SAL方法获取的AdSCs（SAL组）和采用UAL方法获取的AdSCs（UAL组），水凝胶组只加入水凝胶作为对照，同时比较3组的组织再生能力。结果UAL与SAL方法获得的AdSCs相比，在细胞数量和增殖能力方面的差异无统计学意义。油红O染色显示两者成脂分化能力没有差异，分化7d时，PPAR-γ受体和LPL的表达差异无统计学意义也证实了这一点（P〉0．05）。同样，碱性磷酸酶和茜素红染色也显示了两者相似的成骨分化能力，而且成骨标志物Runx-2、骨桥蛋白和骨钙蛋白的表达差异也无统计学意义（P〉0．05）。SAL组和UAL组的愈合速度明显大于水凝胶组（P〈0．05），SAL和UAL两组之间差异无统计学意义（P〉0．05）。结论与传统SAL方法相比，UAL方法可获得相等增殖能力的AdSCs，并用于以细胞疗法为基础的治疗。

关键词：细胞再生能力脂肪抽吸吸脂超声 AdSCs 脂肪来源干细胞成骨分化整形外科医师

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