检索结果-南通市图书馆

JAK2 Mutations in Chronic Myeloproliferative Neoplasm;Towards the Application of Personalized Treatments for Saudi Patients

引用

Open Journal of Blood Diseases 2012年第2期2卷 17-21页

作者： Mamdooh Gari Fatin Al-Sayes Farid Ahmed Abdul Ali Peerzada adel abuzenadah Lubna S. Mira Mohammed Al-Qahtani Ashraf Dallol Adeel Chaudhary Mohammed Mahnashi Ghazi Damanhouri Center of Excellence in Genomic Medicine Research King Abdulaziz University Jeddah Saudi Arabia Clinical Hematology Laboratory King Abdulaziz Hospital and Oncology Center Jeddah Saudi Arabia Department of Hematology Faculty of Medicine King Abdulaziz University Jeddah Saudi Arabia Department of Medical Laboratory Technology Faculty of Applied Medical Sciences King Abdulaziz University Jeddah Saudi Arabia

The chronic myeloproliferative neoplasms (CMPN) are a group of clonal hematopoietic stem cell disorders in which large numbers of red blood cells, white blood cells, or platelets grow and spread excess in the bone marrow and the pe- ripheral blood. Cytogenetic analysis of the t (9:22) and molecular detection of BCR/ABL is the main diagnostic criteria in Philadelphia positive CMPN (CML). The identification of non-receptor tyrosine kinase JAK2 mutations (exon 14 JAK2 V617F and exon 12) have significantly contributed to our understanding of the molecular mechanisms in the pathogenesis of Philadelphia negative CMPN such as polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF) patients. According to the revised WHO classification, JAK2 mutation is considered as a major diagnostic and clonal marker in Philadelphia negative CMPN which will play a major role in designing personal- ized treatments for the disease. JAK2 V617F mutation frequency is unknown in Saudi Arabia. Therefore, investigation of the JAK2 V617F mutation was carried out in DNA samples from 78 peripheral blood specimens corresponding to patients with polycythemia vera (PV) (n = 11), Chronic myeloid leukemia (CML) (n = 45), essential thrombocythemia (ET) (n = 10), idiopathic myelofibrosis (MF) (n = 12). We used polymerase chain reaction and direct DNA sequencing to detect the JAK2 mutation. Overall, the incidence of the JAK2 V617F mutation was 91% in PV, 40% in ET, and 25% in MF. This approach proved to be reliable and more sensitive in detecting the mutation. Two essential findings arose from our study. First, this technique could be carried out with DNA samples, even partially degraded, from routinely processed BM or peripheral blood specimens. Second, after correlation with morphological features, it turned out that the characteristics of the megakaryocytes were more specific than the mutational status of JAK2 in characterizing ET and PMF. Concerning PV, as expected, the in

关键词： Leukemia CMPN PV ET PMF JAK2 Mutation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bacteriophages in <i>Escherichia coli</i>antimicrobial resistance

引用

Advances in Bioscience and Biotechnology 2013年第3期4卷 469-476页

作者： Archana Iyer Elie Barbour Esam Azhar Ishtiaq Qadri Adeel Chaudhary adel abuzenadah Taha Kumosani Ghazi Damanhouri Suhad Bahijri Ahmed Al-Hejin Afif M. Abdel Nour Steve Harakeh Department of Animal and Veterinary Sciences American University of Beirut Beirut Lebanon Department of Biochemistry King Abdulaziz University Jeddah KSA King Fahd Medical Research Center King Abdulaziz University Jeddah KSA Medical Laboratory Technology Department Faculty of Applied Medical Sciences King Fahd Medical Research Center King Abdulaziz University Jeddah KSA Special Infectious Agents Unit—Biosafety Level 3 King Fahd Medical Research Center King Abdulaziz University Jeddah KSA

The continuous battle between humans and the multitude of pathogenic microorganisms in the environment has sought relief in the form of antimicrobials. But the counter attack by pathogenic organisms in the form of multidrug resistance, acquired by various mechanisms such as transformation, transposition, conjugation and transduction is a major reason for concern. Bacteriophages have contributed in a significant way to dissemination of genes encoding antimicrobial resistance, heavy metal resistance and virulence factors through the phenomenon of transduction. This review aims at compiling information about the different mechanisms by which bacteriophages aid in transferring genes involved in antimicrobial resistance to Escherischia coli in various environments.

关键词： Bacteriophages Escherischia coli Antimicrobial Resistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transposable elements in <i>Escherichia coli</i>antimicrobial resistance

引用

Advances in Bioscience and Biotechnology 2013年第3期4卷 415-423页

作者： Archana Iyer Elie Barbour Esam Azhar Alaaeddin Ali El Salabi Hani Mutlak A. Hassan Ishtiaq Qadri Adeel Chaudhary adel abuzenadah Taha Kumosani Ghazi Damanhouri Maha Alawi Tarek Na’was Afif M. Abdel Nour Steve Harakeh Department of Animal and Veterinary Sciences American University of Beirut Beirut Lebanon Department of Biochemistry King Abdulaziz University Jeddah KSA Infection Control and Antimicrobial Drug Resistance University of Benghazi Benghazi Libya Infection Control and Environmental Health Unit King Abdulaziz University Hospital King Abdulaziz University Jeddah KSA King Fahd Medical Research Center King Abdulaziz University Jeddah KSA Lebanese American University Beirut Lebanon Special Infectious Agents Unit—Biosafety Level 3 King Fahd Medical Research Center King Abdulaziz University Jeddah KSA

Transposable elements are capable of switching their positions on the genome thereby causing gene arrangements and contributing to genome evolution. The aim of this review is to specifically discuss the role of transposable elements in transferring antimicrobial resistance genes in E. coli, thus contributing to increase in virulence and conferring the possibility of multidrug resistance. Different types of transposable elements such as transposons and integrons and their profound influence on E. coli antimicrobial resistance are the focus of this review.

关键词： Transposable Elements E. coli Antimicrobial Resistance Integrons

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Assessment of Biochemical and Antioxidative Status in Patients Suffering from Dengue Fever

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2015年第3期35卷 411-418页

作者： Mahmood Rasool Arif Malik Khalid Mahmud Khan Muhammad Saeed Qureshi Beenish Shabbir Sara Zahid Muhammad Asif Abdul Manan Sana Rashid Saima Rubab Khan Hafiz Muhammad Arsalan Rabail Alam Mahwish Arooj Mahmood Husain Qazi Adeel Gulzar Ahmed Chaudhary adel Mohammed abuzenadah Mohammed Hussain Al-Qahtani Sajjad Karim Center of Excellence in Genomic Medicine Research Faculty of Applied Medical SciencesKing Abdulaziz University Institute of Molecular Biology and Biotechnology the University of Lahore Fatima Jinnah Medical College Sir Gangaram Hospital Lahore Institute of Molecular Biology and Biotechnology the University of Lahore Department of Biotechnology and Informatics BUITEMS Quetta 87300 Pakistan Center for Research in Molecular Medicine the University of Lahore KACST Technology Innovation Center in Personalized Medicine King Abdulaziz University Center of Excellence in Genomic Medicine Research Faculty of Applied Medical Sciences King Abdulaziz University

Summary：A multi-centred study was designed to collect dengue epidemiologic data from government and registered private hospitals/clinics and maintained archive of frozen specimens in bio-bank to be used for future dengue epidemic control program, and assess the epidemiology of dengue fever（DF） by evaluating biochemical and oxidative status of patients. ELISA Ig M antibodies test was done to confirm DF. From August 2010 to December 2011, 101 confirmed blood samples of DF patients referred to pathology lab of Jinnah Hospital Lahore were subjected to the epidemiologic assessment by evaluating the biochemical and physiological indices and alterations of circulating antioxidants. Clinical features of DF patients and effect of fever on blood components and serum proteins of liver were recorded. The hospital stay in DF, dengue hemorrhagic fever（DHF） and dengue shock syndrome（DSS） showed significant difference. Significant increases in serum alanine amino transferase（ALT）（P=0.000）, aspartate amino transferase（AST）（P=0.000）, alkaline phosphatase（ALP）（P=0.000）, malondialdehyde（MDA） along with significant decreases in total protein（TP）（P=0.000）, reduced glutathione（GSH）（P=0.000）, superoxide dismutase（SOD）, catalase（CAT）（P=0.000）, and sialic acid contents（P=0.016） were observed. A positive correlation existed between bound sialic acid levels, liver enzymes and circulating antioxidants（r=0.656, P=0.016）. In the present study, alterations of circulating antioxidants in DF suggest that DF might be a metabolic response to an acute, self-limiting tropical viral infection, and a consequence of the viral inflammatory process.

关键词： dengue fever alanine amino transferase aspartate amino transferase malondialdehyde superoxide dismutase catalase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：