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Proper Holomorphic Maps from Domains in C2 with Transverse Circle Action

Chinese Annals of Mathematics,Series B 2007年第5期28卷 533-542页

作者： adam coffman Yifei PAN Department of Mathematical Sciences Indiana University - Purdue University Fort Wayne Fort Wayne IN 46805-1499 USA. Department of Mathematical Sciences Indiana University - Purdue University Fort Wayne Fort Wayne IN 46805-1499 USA College of Mathematics and Information Sciences Jiangxi Normal University Nanchang 330022 China.

The authors consider proper holomorphic mappings between smoothly bounded pseudoconvex regions in complex 2-space,where the domain is of finite type and admits a transverse circle *** main result is that the closure of each irreducible component of the branch locus of such a map intersects the boundary of the domain in the union of finitely many orbits of the group action.

关键词： Proper holomorphic map Pseudoconvex domain

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Non-transgenic Plant Genome Editing Using Purified Sequence-Specific Nucleases

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Molecular Plant 2015年第9期8卷 1425-1427页

作者： Song Luo Jin Li Thomas J. Stoddard Nicholas J. Baltes Zachary L. Demorest Benjamin M. Clasen Andrew coffman adam Retterath Luc Mathis Daniel F. Voytas Feng Zhang Cellectis Plant Sciences 600 County Road D West Suite 8 New Brighton MN 55112 USA

Dear Editor Sequence-specific nucleases, including zinc-finger nucleases, meganucleases, TAL effector nucleases （TALENs）, and CRISPR/ Cas systems, have been used to introduce targeted mutations in a wide range of plant species （Voytas, 2013; Baltes and Voytas, 2015）. However, delivery of these nucleases using traditional transformation methods （e.g., particle bombardment, Agrobacterium or protoplast transformation） may result in undesired genetic alterations due to random insertion of nucleaseencoding DNA into the host genome. Random integration of DNA is a particular concern for trait improvement and gene function studies, because it may lead to unintended gene inactivation or it may alter expression of host genes. Furthermore, for crop varieties created using biotechnology, the presence of foreign DNA is a trigger for regulation by many governmental agencies （Voytas and Gao, 2014）. Therefore, methods to modify plant genomes that do not require DNA delivery would have value in both commercial and academic settings. Here we demonstrate nontransgenic plant genome engineering by introducing sequencespecific nucleases as purified protein. This approach enabled targeted mutagenesis of endogenous sequences within plant cells, while avoiding integration of foreign DNA into the genome.

关键词： Non-transgenic Plant Genome

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