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Steatosis as a co-factor in chronic liver diseases

World Journal of Gastroenterology 2010年第10期16卷 1171-1176页

作者： Marcello Persico achille iolascon Department of Internal Medicine Second University of Naples Department of Biochemistry and Medical Biochemistry University Federico II

The finding of lipid accumulation in the liver, so-called hepatic steatosis or non-alcoholic fatty liver disease, is a common condition frequently found in healthy subjects. Its prevalence, in fact, has been estimated by magnetic resonance studies to be about 35% in the general population and 75% in obese persons. Nevertheless, its presence generates liver damage only in a small percentage of subjects not affected by other liver diseases. It should be defined as a "co-factor" capable of affecting severity and progression, and also therapeutic perspectives, of liver diseases to which it is associated. Herein we will evaluate the impact of hepatic steatosis and obesity on the most common liver diseases: chronic viral hepatitis C and B, and alcoholic liver disease.

关键词： Liver Steatosis Non-alcoholic fatty liver disease

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Tomographic flow cytometry by digital holography

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Light(Science & Applications) 2016年第1期5卷 128-134页

作者： Francesco Merola Pasquale Memmolo Lisa Miccio Roberto Savoia Martina Mugnano Angelo Fontana Giuliana D’Ippolito Angela Sardo achille iolascon Antonella Gambale Pietro Ferraro CNR-ISASI Istituto di Scienze Applicate e Sistemi Intelligenti‘E.Caianiello’CNR-Consiglio Nazionale delle RicerchePozzuoli 80078Italy CNR-ICB Istituto di Chimica BiomolecolarePozzuoli 80078Italy Department of Molecular Medicine and Medical Biotechnology University of Naples Federico II&CEINGE-Advanced BiotechnologiesNapoli 80145Italy

High-throughput single-cell analysis is a challenging ***-free tomographic phase microscopy is an excellent candidate to perform this ***,in-line tomography is very difficult to implement in practice because it requires a complex set-up for rotating the sample and examining the cell along several *** demonstrate that by exploiting the random rolling of cells while they are flowing along a microfluidic channel,it is possible to obtain in-line phase-contrast tomography,if smart strategies for wavefront analysis are *** fact,surprisingly,a priori knowledge of the three-dimensional position and orientation of rotating cells is no longer needed because this information can be completely retrieved through digital holography wavefront numerical *** approach makes continuous-flow cytotomography suitable for practical operation in real-world,single-cell analysis and with a substantial simplification of the optical system;that is,no mechanical scanning or multi-direction probing is required.A demonstration is given for two completely different classes of biosamples:red blood cells and diatom *** accurate characterization of both types of cells is reported,despite their very different nature and material content,thus showing that the proposed method can be extended by adopting two alternate strategies of wavefront analysis to many classes of cells.

关键词： microfluidics red blood cells tomographic microscopy wavefront analysis

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