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Metabolomics Study in Methylotrophic Yeast： A Minireview

Journal of Life Sciences 2017年第1期11卷 11-18页

作者： Jye Ping Fam Suriana Sabri Syarul Nataqain Baharum abu bakar salleh Siti Nurbaya Oslan Enzyme and Microbial Technology Research Centre Universiti Putra Malaysia Serdang 43400 Selangor Malaysia Department of Biochemistry Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia Serdang 43400Selangor Malaysia Department of Microbiology Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia Serdang 43400Selangor Malaysia Institute of Systems Biology Universiti Kebangsaan Malaysia Bangi 43600 Selangor Malaysia

Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX （alcohol oxidase） protein which has been characterised to be a strong and tightly methanol-inducible dependent promoter. Metabolomics is the systematic study and inclusive analysis of small molecules called metabolites in a biological system. Metabolomics plays an important part in connecting the phenotype and genotype gap because it magnifies the modifications in the proteome and provides a better phenotype representation of an organism. This quantitative study has provided a new perception on the metabolic burden derived from the overexpression of recombinant protein in methylotrophic yeast. In this review, we discuss the fundamental aspect of metabolomics in methylotrophic yeast followed by their latest developments.

关键词： Methylotrophic yeast metabolomics methanol utilisation pathway.

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Engineering catalytic efficiency of thermophilic lipase from <i>Geobacillus zalihae</i>by hydrophobic residue mutation near the catalytic pocket

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Advances in Bioscience and Biotechnology 2012年第2期3卷 158-167页

作者： Roswanira Abdul Wahab Mahiran Basri Mohd Basyaruddin Abdul Rahman Raja Noor Zaliha Raja Abdul Rahman abu bakar salleh Leow Thean Chor 3Institute of Bioscience Universiti Putra Malaysia Serdang Malaysia 4Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia Serdang Malaysia Faculty of Science Universiti Putra Malaysia Serdang Malaysia Faculty of Science Universiti Teknologi Malaysia Skudai Malaysia

In-silico and experimental investigations were conducted to explore the effects of substituting hydrophobic residues;Val, Met, Leu, Ile, Trp and Phe into the oxyanion Q114 of T1 lipase. We hypothesized that the oxyanion Q114, involved in substrate binding is also associated with modulation of conformational stability and in conferring specific enzyme attributes. The insilico investigations accurately predicted the quality of the protein packing in some of the variants. Our study found by altering the hydrophobicity of the oxyanion 114, remarkably altered enzyme conformational stability and catalytic attributes. Substitution with Leu resulted improvements in four out of the six tested characteristics. The hydrophobic Leu might have improved local structure folding and increased hydrophobic interactions with other residues in the vicinity of the mutation. The Met variant showed higher activity over the wild-type in hydrolyzing a wider range of natural oils. The bulky amino acids, Phe and Trp negatively affected T1 lipase and resulted in the largest disruption of protein stability and inferior enzyme characteristics. We have successfully illustrated that a single point residue changes at oxyanion 114 could result in a myriad of enzyme attributes, which implied there was some interplay between hydrophobicity and conformation for lipase catalytic functions.

关键词： Hydrophobic Residue T1 Lipase Oxyanion Conformational Stability Catalytic Pocket

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Preparation of Layered Double Hydroxide-Immobilized Lipase for High Yield and Optically Active (-)-Menthyl butyrate

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复旦学报（自然科学版） 2007年第5期46卷 778-页

作者： Siti Salhah Othman Mahiran Basri Mohd.Zobir Hussein Mohd Basyaruddin Abdul Rahman Raja Noor Zaliha Raja Abdul Rahman abu bakar salleh Salina Mat Radzi Azwani Sofia Ahmad Khiar Food Biotechnology Programme Universiti Sains Islam Malaysia Department of Chemistry Faculty of ScienceUniversiti Putra Malaysia Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia

1 Results Layered Double Hydroxide (LDH) finds extensive usage in the areas of pharmaceutical sciences and catalysis. In this study, a member of the LDH family, Mg/Al-hydrotalcite (HT), or the so-called anionic clay, was prepared at ratio 4 (HT) by co-precipitating through continuous agitation. X-ray diffraction pattern and thermogravimetric analysis of the material indicated that a pure HT had been successfully synthesized. This matrix was then used as support in the immobilization of lipase from Cand...

关键词： LDH-lipase hydrotalcite candida rugosa enantioselective esterification (±)-menthylbutyrate

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马来西亚番荔枝科紫玉盘属(Uvaria Linn.)、杯冠木属(Cyathostemma Griff.)和 Ellipeia Hook. f. et Thomson叶的比较解剖学(英文)

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热带亚热带植物学报 2000年第3期8卷 215-224,T001,T004页

作者： Khatijah Haji HUSSIN Noraishah abu SAMAH Kamarudin MAT-salleh School of Environmental and Resource Science Studies Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia43600 BangiSelangorMalaysia

对紫玉盘属８种，杯冠木属３种和Ｅｌｌｉｐｅｉａ属１种进行了叶的比较研究，以调查不同种之间解剖学的不同点，这对种的鉴别和了解它们的分类学意义可能是有用的。有意义的解剖学特征是：末端石细胞，表皮细胞的晶簇，毛状体，在... 详细信息

对紫玉盘属８种，杯冠木属３种和Ｅｌｌｉｐｅｉａ属１种进行了叶的比较研究，以调查不同种之间解剖学的不同点，这对种的鉴别和了解它们的分类学意义可能是有用的。有意义的解剖学特征是：末端石细胞，表皮细胞的晶簇，毛状体，在中脉和叶柄的薄壁组织内的短石细胞以及中脉和叶柄横切面的形状。有某些特征仅出现在某些种中，这对种的鉴别是有用的。结果亦显示这三个属的联系十分紧密。

关键词：紫玉盘属杯冠木属叶比较解剖学番荔枝科

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