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Number of COVID-19 cases required in a populat.on t. det.ct.SARS-CoV-2 RNA in wast.wat.r in t.e province of Albert., Canada:Sensit.vit. assessment

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Journal of Environment.l Sciences 2023年第3期125卷 843-850页

作者： Qiaozhi Li Bonit. E.Lee t.ejun Gao Yuanyuan Qiu Erik Ellehoj Jiaao Yu mathew Diggle Graham t.pples Rasha Maal-Bared Deena Hinshaw Christ.pher Sikora Nicholas J.Ashbolt.James t.lbot.St.ve E.Hrudey Xiaoli Pang School of Public Healt. University of AlbertaEdmontonAB T6G 2R3Canada Depart.ent.of Pediat.ics University of AlbertaEdmontonAB T6G 1C9Canada Depart.ent.of Laborat.ry Medicine and Pat.ology University of AlbertaEdmontonAB T6G 2B7Canada Universit. of Albert. Cent.al Receiving EdmontonAlbertaT6G 2R3Canada Provincial Laborat.ry for Public Healt. EdmontonAlbertaCanada EPCOR EdmontonAB T5H 0E8Canada Depart.ent.of Medicine University of AlbertaEdmontonAB T6G 2G3Canada Facult. of Science and Engineering Southern Cross UniversityEast Lismore NSW 2480Australia

Wit. a unique and large size of t.st.ng result. of 1,842 samples collect.d from 12 wast.wat.r t.eat.ent.plant. (WWt.) for 14 mont.s t.rough from low t. high prevalence of COVID-19,t.e sensit.vit. of Rt.qPCR det.ct.on of SARS-CoV-2 RNA in wast.wat.r t.at.correspond t. t.e communit.es was comput.d by using Probit.*** st.dy det.rmined t.e number of new COVID-19 cases per 100,000 populat.on required t. det.ct.SARS-CoV-2RNA in wast.wat.r at.defined probabilit.es and provided an evidence-based framework of wast.wat.r-based epidemiology surveillance (WBE).Input.dat. were posit.ve and negat.ve t.st.result. of SARS-CoV-2 RNA in wast.wat.r samples and t.e corresponding new COVID-19 case rat.s per 100,000 populat.on served by each *** analyses det.rmined t.at.Rt.qPCR-based SARS-CoV-2 RNA det.ct.on t.reshold at.50%,80%and 99%probabilit. required a median of 8 (range:4-19),18 (9-43),and 38 (17-97) of new COVID-19 cases/100,000,***,t.e posit.ve det.ct.on rat. at.50%,80%and 99%probabilit. were 0.01%,0.02%,and 0.04%averagely for new cases in t.e *** st.dy improves underst.nding of t.e performance of WBE SARS-CoV-2 RNA det.ct.on using t.e large dat.set. and prolonged st.dy *** COVID-19 burden at.a communit. level t.at.would result.in a posit.ve det.ct.on of SARS-CoV-2 in wast.wat.r is crit.cal t. support.WBE applicat.on as a supplement.ry warning/monit.ring syst.m for COVID-19 prevent.on and cont.ol.

关键词： SARS-CoV-2 COVID-19 cases Wast.wat.r Probit.analysis Probabilit. Det.ct.on sensit.vit.

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Preliminary st.dy on overproduct.on of react.ve oxygen species by neut.ophils in diabet.s mellit.s

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World Journal of Diabet.s 2016年第13期7卷 271-278页

作者： Noridzzaida Ridzuan Cini mathew John Prat.eep Sandrasaigaran Maryam Maqbool Lee Chuen Liew Jonat.an Lim Rajesh Ramasamy Immunology Laborat.ry Department of PathologyFaculty of Medicine and Health Sciences Universiti Putra Malaysia Serdang 43400 Selangor Malaysia St.m Cell Research Laborat.ry Genetic and Regenerative Medicine Research Center Faculty of Medicine and Health Sciences Universiti Putra Malaysia Serdang 43400 SelangorMalaysia Depart.ent.of Physiology and Pharmacology Facult. of Medicine University of Calgary CalgaryAB T2N 4N1 Canada Depart.ent.of Medicine Faculty of Medicine and Health Sciences Universiti Putra Malaysia Serdang 43400Selangor Malaysia

AIM:t. assess t.e amount.and pat.ern of react.ve oxygen species(ROS)product.on in diabet.c pat.ent.erived ***:Blood samples from t.pe 2 diabet.s mellit.s(DM)pat.ent. and volunt.ers(cont.ols)were subject.d t. neut.ophil isolat.on and t.e assessment.of neut.ophil oxidat.ve burst.using chemiluminescence *** were act.vat.d by using phorbol myrist.t. acet.t.(PMA)and neut.ophils wit.out.act.vat.on were kept.as a negat.ve *** chemilu-minescence readings were obt.ined by t.ansferring cell suspension int. a 1.5 m L Eppendorf t.be,wit. PMA and *** mixt.res were gent.y vort.xed and placed inside luminomet.r for a durat.on of 5 ***:Our result. showed t.at.in t.e rest.ng condit.on,t.e secret.on of ROS in normal non-diabet.c individuals was relat.vely low compared t. diabet.c ***,t.e t.me scale observat.on revealed t.at.t.e secret.d ROS declined accordingly wit. t.me in non-diabet.c individuals,yet.such a reduct.on was not.det.ct.d in diabet.c pat.ent. where at.all t.e t.me point.,t.e secret.on of ROS was maint.ined at.similar *** preliminary st.dy demonst.at.d t.at.ROS product.on was significant.y higher in pat.ent. wit. DM compared t. non-diabet.c subject. in bot. rest.ng and act.vat.d ***:t.e respirat.ry burst.act.vit. of neut.ophils could be affect.d by DM and t.e elevat.on of ROS product.on might.be an aggravat.ng fact.r in diabet.c-relat.d complicat.ons.

关键词： Neut.ophils Diabet.s mellit.s React.ve oxygen species Chemiluminescence

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