检索结果-南通市图书馆

Tppp3~+synovial/tendon sheath progenitor cells contribute to heterotopic bone after trauma

在线全文

学校读者我要写书评

暂无评论

Bone Research 2023年第3期11卷 548-559页

作者： Ji-Hye Yea Mario Gomez-Salazar Sharon Onggo Zhao Li Neelima Thottappillil Masnsen Cherief Stefano Negri Xin Xing Qizhi Qin Robert Joel Tower Chen-Ming Fan Benjamin Levi aaron w.james Department of Pathology Johns Hopkins UniversityBaltimoreMD 21205USA Orthopaedic and Trauma Surgery Unit Department of SurgeryDentistryPaediatrics and Gynaecology of the University of VeronaVeronaItaly Center for Organogenesis and Trauma Department of SurgeryUniversity of Texas SouthwesternDallasTXUSA Carnegie Institution for Science BaltimoreMDUSA

Heterotopic ossification(HO)is a pathological process resulting in aberrant bone formation and often involves synovial lined *** this process,mesenchymal progenitor cells undergo endochondral ***,the specific cell phenotypes and mechanisms driving this process are not well understood,in part due to the high degree of heterogeneity of the progenitor cells ***,using a combination of lineage tracing and single-cell RNA sequencing(sc RNA-seq),we investigated the extent to which synovial/tendon sheath progenitor cells contribute to heterotopic bone *** this purpose,Tppp3(tubulin polymerization-promoting protein family member 3)-inducible reporter mice were used in combination with either Scx(Scleraxis)or Pdgfra(platelet derived growth factor receptor alpha)reporter *** tendon injury-and arthroplasty-induced mouse experimental HO models were *** RNA-seq of tendon-associated traumatic HO suggested that Tppp3 is an early progenitor cell marker for either tendon or osteochondral *** HO induction,Tppp3 reporter^(+)cells expanded in number and partially contributed to cartilage and bone formation in either tendon-or joint-associated *** double reporter animals,both Pdgfra^(+)Tppp3^(+)and Pdgfra^(+)Tppp3^(-) progenitor cells gave rise to HO-associated ***,analysis of human samples showed a substantial population of TPPP3^(-) expressing cells overlapping with osteogenic markers in areas of heterotopic ***,these data demonstrate that synovial/tendon sheath progenitor cells undergo aberrant osteochondral differentiation and contribute to HO after trauma.

关键词： progenitor tendon reporter

Neuron-to-vessel signaling is a required feature of aberrant stem cell commitment after soft tissue trauma

在线全文

学校读者我要写书评

暂无评论

Bone Research 2022年第3期10卷 615-626页

作者： Qizhi Qin Mario Gomez-Salazar Masnsen Cherief Chase A.Pagani Seungyong Lee Charles Hwang Robert J.Tower Sharon Onggo Yuxiao Sun Abhinav Piplani Zhao Li Sowmya Ramesh Thomas L.Clemens Benjamin Levi aaron w.james Department of Pathology Johns Hopkins UniversityBaltimoreMD 21205USA Center for Organogenesis and Trauma Department of SurgeryUniversity of TexasSouthwesternTXUSA Department of Plastic Surgery HarvardCambridgeMA 02138USA Department of Orthopaedics Johns Hopkins UniversityBaltimoreMD 21205USA Baltimore Veterans Administration Medical Center BaltimoreMD 21201USA

The functional interdependence of nerves and blood vessels is a well-established concept during tissue morphogenesis, yet the role of neurovascular coupling in proper and aberrant tissue repair is an emerging field of interest. Here, we sought to define the regulatory relationship of peripheral nerves on vasculature in a severe extremity trauma model in mice, which results in aberrant cell fate and heterotopic ossification(HO). First, a high spatial degree of neurovascular congruency was observed to exist within extremity injury associated heterotopic ossification. Vascular and perivascular cells demonstrate characteristic responses to injury,as assessed by single cell RNA sequencing. This vascular response to injury was blunted in neurectomized mice, including a decrease in endothelial proliferation and type H vessel formation, and a downregulation of key transcriptional networks associated with angiogenesis. Independent mechanisms to chemically or genetically inhibit axonal ingrowth led to similar deficits in HO site angiogenesis, a reduction in type H vessels, and heterotopic bone formation. Finally, a combination of single cell transcriptomic approaches within the dorsal root ganglia identified key neural-derived angiogenic paracrine factors that may mediate neuron-to-vascular signaling in HO. These data provide further understanding of nerve-to-vessel crosstalk in traumatized soft tissues, which may reflect a key determinant of mesenchymal progenitor cell fate after injury.

关键词： aberrant trauma nerves

Comparison of skeletal and soft tissue pericytes identifies CXCR4+ bone forming mural cells in human tissues

在线全文

学校读者我要写书评

暂无评论

Bone Research 2020年第3期8卷 286-299页

作者： Jiajia Xu Dongqing Li Ching-Yun Hsu Ye Tian Leititia Zhang Yiyun wang Robert J.Tower Leslie Chang Carolyn A.Meyers Yongxing Gao Kristen Broderick Carol Morris Jody E.Hooper Sridhar Nimmagadda Bruno Peault aaron w.james Departments of Pathology Johns Hopkins UniversityBaltimore 21205 MDUSA Department of Microbiology Wuhan University School of Basic Medical of ScienceWuhanHubeiP.R.China Department of Oral and Maxillofacial Surgery School of StomatologyChina Medical UniversityShenyangLiaoningP.R.China Departments of Orthopaedics Johns Hopkins UniversityBaltimore 21205 MDUSA Departments of Plastic Surgery Johns Hopkins UniversityBaltimore 21205 MDUSA Departments of Autopsy Service Johns Hopkins UniversityBaltimoreMD 21205USA Departments of Radiology and Radiological Science bonBaltimore 21205 MDUSA UCLA and Orthopaedic Hospital Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center Los AngelesCA 90095USA Center for Cardiovascular Science and MRC Center for Regenerative Medicine University of EdinburghEdinburghUK

Human osteogenic progenitors are not precisely defined,being primarily studied as heterogeneous multipotent cell populations and termed mesenchymal stem cells(MSCs).Notably,select human pericytes can develop into bone-forming ***,we sought to define the differentiation potential of CD146 f human pericytes from skeletal and soft tissue sources,with the underlying goal of defining cell surface markers that typify an osteoblastogenic ***146+CD31~CD45_pericytes were derived by fluorescence-activated cell sorting from human periosteum,adipose,or dermal *** CD146+CD31—CD45 cells retained canonical features of pericytes/*** pericytes demonstrated a striking tendency to undergo osteoblastogenesis in vitro and skeletogenesis in vivo,while soft tissue pericytes did not *** analysis revealed higher CXCR4 signaling among periosteal pericytes in comparison to their soft tissue counterparts,and CXCR4 chemical inhibition abrogated ectopic ossification by periosteal ***,enrichment of CXCR4+pericytes or stromal cells identified an osteoblastic/non-adipocytic precursor *** sum,human skeletal and soft tissue pericytes differ in their basal abilities to form *** exists in soft tissue pericytes,however,and CXCR4+pericytes represent an osteoblastogenic,non-adipocytic cell ***,enrichment for CXCR4-expressing stromal cells is a potential new tactic for skeletal tissue engineering.

关键词： CD146 CXCR4 pericyte

The HIF-1α/PLOD2 axis integrates extracellular matrix organization and cell metabolism leading to aberrant musculoskeletal repair

在线全文

学校读者我要写书评

暂无评论

Bone Research 2024年第2期12卷 289-301页

作者： Heeseog Kang Amy L.Strong Yuxiao Sun Lei Guo Conan Juan Alec C.Bancroft Ji Hae Choi Chase A.Pagani Aysel A.Fernandes Michael woodard Juhoon Lee Sowmya Ramesh aaron w.james David Hudson Kevin N.Dalby Lin Xu Robert J.Tower Benjamin Levi Center for Organogenesis Regeneration and TraumaDepartment of SurgeryUniversity of Texas SouthwesternDallasTX 75390USA Section of Plastic Surgery Department of SurgeryUniversity of MichiganAnn ArborMI 48109USA Quantitative Biomedical Research Center Peter O’Donnell Jr.School of Public HealthUniversity of Texas SouthwesternDallasTX 75390USA Department of Orthopedics and Sports Medicine University of WashingtonSeattleWA 98195USA Division of Chemical Biology and Medicinal Chemistry College of PharmacyUniversity of Texas at AustinAustinTX 78712USA Department of Pathology Johns Hopkins UniversityBaltimoreMD 21218USA

while hypoxic signaling has been shown to play a role in many cellular processes,its role in metabolism-linked extracellular matrix(ECM)organization and downstream processes of cell fate after musculoskeletal injury remains to be ***(HO)is a debilitating condition where abnormal bone formation occurs within extra-skeletal *** andhypoxia-inducible factor 1α(HIF-1α)activation have been shown to promote ***,the underlying molecular mechanisms bywhich the HIF-1αpathway in mesenchymal progenitor cells(MPCs)contributes to pathologic bone formation remain to ***,we used a proven mouse injury-induced HO model to investigate the role of HIF-1αon aberrant cell ***-cell RNA sequencing(scRNA-seq)and spatial transcriptomics analyses of the HO site,we found that collagen ECM organizationis the most highly up-regulated biological process in *** mesenchymal cell-specific deletion of Hif1α(Hoxa11-CreER^(T2);Hif1a^(fl/fl))significantly mitigated HO in ***-seq analysis of these Hoxa11-CreER^(T2);Hif1a^(fl/fl)mice identified the PLOD2/LOXpathway for collagen cross-linking as downstream of the HIF-1αregulation of ***,our scRNA-seq data and mechanisticstudies further uncovered that glucose metabolism in MPCs is most highly impacted by HIF-1α*** a translational aspect,a pan-LOX inhibitor significantly decreased HO.A newly screened compound revealed that the inhibition of PLOD2 activity in MPCssignificantly decreased osteogenic differentiation and glycolytic *** suggests that the HIF-1α/PLOD2/LOX axis linked tometabolism regulates HO-forming MPC *** results suggest that the HIF-1α/PLOD2/LOX pathway represents a promisingstrategy to mitigate HO formation.

关键词： metabolism skeletal aberrant

Foot reflexology in autoimmune diseases:Effectiveness and mechanisms

在线全文

学校读者我要写书评

暂无评论

world Journal of Clinical Cases 2025年第7期13卷 1-5页

作者： Jozélio Freire de Carvalho aaron Lerner Carina Benzvi Núcleo de Pesquisa em Doenças Crônicas não Transmissíveis Federal University of BahiaSalvador 40231-300BahiaBrazil Department of Research Chaim Sheba Medical CenterThe Zabludowicz Research Center for Autoimmune DiseasesRamat Gan 52621Israel

Foot reflexology(FR)is a Chinese-originated and non-invasive complementary therapy increasingly used by functional,alternative and para-medical *** attempts are made to study FR in non-functional organic *** present invited Editorial discusses the application of FR in autoimmune diseases(AD),highlighting a few successful studies demonstrating symptomatic relief and objective *** promising results,the FR domain remains under-investigated and an urgent need to confirm and understand the effect of FR in chronic diseases,including AD,is highly recommended.

关键词： Reflexology Foot reflexology Sensorineural hearing loss Autoimmune diseases Autoimmunity

Application of rare earths in fluid catalytic cracking： A review

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rare Earths 2017年第10期35卷 941-956页

作者： aaron Akah Research and Development Center Saudi Aramco Dhahran 31311 Saudi Arabia

The need for more active and hydrothermally stable fluid catalytic cracking（FCC）catalysts to combat the effect of metal contaminants has led to an increase in demand for rare earth *** earth oxides enhance catalyst activity and prevent the loss of acid sites during the FCC unit operation,especially when heavy residue with high metal content is used as *** this paper,a review was carried out to show the effects of rare earth elements on the structure,activity,and stability of FCC ***,the use of rare earth elements as vanadium traps was analyzed in conjunction with the mechanism of catalyst deactivation by *** objective was to elucidate the interaction of vanadium species with the zeolite component of the FCC catalysts and the role of rare earth elements in countering the deleterious effects of vanadium on the FCC catalysts.

关键词： FCC catalyst rare earth vanadium trap zeolites

Comparison of three regionalization techniques for predicting streamflow in ungaged watersheds in Nebraska,USA using SwAT model

在线全文

学校读者我要写书评

暂无评论

International Journal of Agricultural and Biological Engineering 2018年第3期11卷 110-119页

作者： Michael w.Van Liew aaron R.Mittelstet Department of Biological Systems Engineering University of NebraskaLincolnNE 68583USA

This study compared three approaches,regional averaging,nearest neighbor,and donor techniques,to regionalize parameters in the Soil and water Assessment Tool(SwAT)on eleven watersheds located in the Dissected Plains,Plains,and Rolling Hills Landforms in the eastern portion of the State of Nebraska,*** the Rolling Hills Landform,three watersheds were randomly selected as calibration watersheds while two were randomly selected as validation *** watersheds were randomly selected as calibration watersheds while one was randomly selected as a validation watershed within each of the Dissected Plains and Plains *** seven calibration watersheds were used to provide the necessary calibrated parameter sets to execute each of the regional approaches,while the four validation watersheds were used to assess the impact of applying each of these approaches to an uncalibrated *** Bias(PBIAS)and the Nash Sutcliffe Coefficient of Efficiency(NSE)were used to assess model *** results of this study show that all three methods performed poorly,since the majority of watersheds among each method tested exhibited PBIAS values greater than ±25% and/or NSE values less than 0.50,which were considered to be unsatisfactory in terms of model *** average regionalization,nearest neighbor and donor methods resulted in only four(two calibration and two validation),zero and one satisfactory set of simulated watershed results,*** findings from this study indicate that although each watershed was successfully calibrated with NSE values ranging from 0.51 to 0.84,none of the three regionalization methods provided suitable calibration data sets to define parameter values for performing satisfactory simulations on ungaged watersheds across the eastern Nebraska landscape.

关键词： SwAT model watersheds hydrology model calibration parameter regionalization Nebraska ungaged watersheds

Closed-loop drip irrigation control using a hybrid wireless sensor and actuator network

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Information Sciences) 2011年第3期54卷 577-588页

作者： FRANZEN aaron Department of Biosystems and Agricultural Engineering Oklahoma State University

In this research, a closed-loop drip irrigation control hybrid wireless sensor and actuator network (HwSAN) prototype were developed and deployed in a crop field for soil property precise measurement and precision irrigation in accordance with the measured soil property. The HwSAN was composed of a wireless sensor and actuator network (wSAN) used for in-field soil property monitoring and irrigation control and a laboratory supervising system. The wSAN included ten sensor nodes, five irrigation control nodes, a central node, a gateway and a cellular modem. The laboratory supervising system was formed by a web-server. The system could automatically sample soil properties including soil moisture, electrical conductivity, and nearsurface temperature at four different depths underground and transmit field data through cellular network to the web-server. Closed-loop control was achieved through the control nodes listening uploading packets from its paired sensor nodes to control drip irrigation. Results from the quality of service (QoS) evaluation included: the data packet transmission rate was 84.76%, data accuracy rate was greater than 97% and no in-field data transmission errors.

关键词： wireless sensor network drip irrigation soil property monitoring precision agriculture

The kinetics of static recovery by dislocation climb

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

npj Computational Materials 2022年第1期8卷 973-982页

作者： aaron A.Kohnert Laurent Capolungo Materials Science and Technology Division Los Alamos National LaboratoryLos AlamosNMUSA

The initial microstructure of a wide range of structural materials is conditioned by thermo-mechanical treatments such as hot-working,tempering,or solution *** the elevated temperatures associated with these treatments the dislocation microstructure evolves,usually decreasing in density through a process known as static *** its technological relevance,static recovery is not fully characterized from a theoretical standpoint,with even the controlling mechanisms subject to *** this study,a climb-enabled discrete dislocation dynamics(DDD)capability is leveraged to explore the kinetics of static recovery in pure Fe when controlled by dislocation *** data from these simulations is used to develop a revised static recovery law,and provides the parameters appropriate for predictive microstructure models in *** law differs from previous analytical derivations invoking climb of dislocations,following the logarithmic trends typical of experimental observations where prior work did *** comparison between the recovery law derived from DDD to experimental recovery data in alpha Fe shows strong agreement across a range of temperatures,and suggests that climb is the controlling mechanism for static recovery in pure metals.

关键词： dislocation law treatment