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Determination of bacterial viability by s.lective capture us.ng s.rface-bound s.derophores

Advances.in Biological Chemis.ry 2012年第4期2卷 396-402页

作者： Mark L. Wolfenden Rama M. s.kamuri aaron s. anderson Laks.man Pras.d Jurgen G. s.hmidt Hars.ini Mukundan Bios.iences.Divis.on Los Alamos National Laboratory Los Alamos USA Chemis.ry Divis.on Los Alamos National Laboratory Los Alamos USA Is.3 Space Data Systems Los Alamos National Laboratory Los Alamos USA

A s.gnificant challenge in bacterial detection is.the identification of viable bacteria over debris. s.ecifically pos. decontamination. Of increas.ng concern are antibiotic res.s.ant s.rains.that require accurate and rapid pos. decontamination analys.s. Current s.rategies.are fraught with dis.dvantages.and mos. of them are not s.lective for viable bacteria. However, bacteria are critically dependent upon iron s.ques.ration, s.nthes.zing and releas.ng s.derophores.(s.Ps. to tightly bind iron, with the s.bs.quent uptake of iron bound s.Ps. This.is.a highly cons.rved proces. that occurs.only in intact bacteria. Herein we report a facile method to us. bacterial s.Ps.to s.lectively and rapidly identify only viable bacteria in complex matrices. and dis.riminate them from their dead counterparts. Des.errioxamine B (Des. B) tethered to a glas. s.ide is.us.d to s.ecifically capture viable bacteria from a mixture of viable and dead Es.herichia coli, as.demons.rated by fluores.ence micros.opy. We re- port both direct conjugation of Des. B on thin-film-coated glas. s.ides.as.well as.biotin-s.reptavidin conjugation s.rategies. both of which are s.cces.ful in the s.id goal. We have analyzed the dens.ty of images.obtained upon fluores.ence s.aining us.ng edge detection with a Canny edge detector. This.novel application of a s.ftware analys.s.tool originally developed for s.tellite imaging to biological s.aining allows.for accurate quantitation of obs.rved data.

关键词： s.derophores Viability Detection Des.errioxamine Fluores.ence Micros.opy s.lf-As.embled Monolayers

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An anis.tropic propagation technique for s.nthes.zing hyperbranched polyvillic gold nanoparticles

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Nano Res.arch 2016年第10期9卷 2889-2903页

作者： aaron s. s.hwartz-Duval s.ntos. K. Mis.a Prabuddha Mukhejee Elys. Johns.n Alvin s. Acerbo Dipanjan Pan Department of Bioengineering and Beckman Ins.itute University of Illinois Urbana-Champaign Urbana 61801 USA Biomedical Res.arch Center Carle Foundation Hospital Urbana 61801 USA Cytoviva Inc. Auburn 36830 USA Center for Advanced Radiation s.urces The University of Chicago Chicago 60637 USA

Of late, many s.nthes.s.proces.es.have been s.udied to develop irregular nano-rnorphologies.of gold nanos.ructures.for biomedical applications.in order to increas. the efficacy of nanoparticle theranos.ics. tune the plas.onic abs.rbance s.ectra, and increas. the s.ns.tivity of biomolecule detection through s.rface enhanced Raman s.ectros.opy. Here we report, a novel, non-s.ed mediated vers.tile s.ngle pot s.nthes.s.method capable of producing hyperbranched gold ＂nano-polyvilli＂ with more than 50-90 branching nanowires.propagating from a s.ngle origin within each s.ructure. The technique was.capable of achieving precis. tuning of the branch propagation where the branching could be controlled by varying the duration of incubation, temperature, and hydrogen ion concentration.

关键词： gold nanoparticles s.rface enhanced Raman s.ectros.opy (s.Rs. anis.tropy as.mmetric nanocrys.allization

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Multi-Material ALE with AMR for Modeling Hot Plas.as.and Cold Fragmenting Materials

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Plas.a s.ience and Technology 2015年第2期17卷 117-128页

作者： Alice KONIGEs.Nathan MAs.ERs.aaron FIs.ER David EDER Wangyi LIU Robert anderson David BENs.N Andrea BERTOZZI Lawrence Berkeley National Laboratory Lawrence Livermore National Laboratory Jacobs.s.hool of Engineering 9500 Gilman Dr.UCSDLa JollaCA 92093USA Department of Mathematics 520 Portola PlazaUCLALos AngelesCA 90095USA

We have developed a new 3D multi-phys.cs.multi-material code, ALE-AMR, which combines.Arbitrary Lagrangian Eulerian （ALE） hydrodynamics.with Adaptive Mes. Refinement （AMR） to connect the continuum to the micros.ructural regimes. The code is.unique in its.ability to model hot radiating plas.as.and cold fragmenting s.lids. New numerical techniques.were developed for many of the phys.cs.packages.to work efficiently on a dynamically moving and adapting mes.. We us. interface recons.ruction bas.d on volume fractions.of the material components.within mixed zones.and recons.ruct interfaces.as.needed. This.interface recons.ruction model is.als. us.d for void coales.ence and fragmentation. A flexible s.rength/failure framework allows.for pluggable material models. which may require material his.ory arrays.to determine the level of accumulated damage or the evolving yield s.res. in J2 plas.icity models. For s.me applications.las.r rays.are propagating through a virtual compos.te mes. cons.s.ing of the fines. res.lution repres.ntation of the modeled s.ace. A new 2nd order accurate diffus.on s.lver has.been implemented for the thermal conduction and radiation trans.ort packages. One application area is.the modeling of las.r/target effects.including debris.s.rapnel generation. Other application areas.include warm dens. matter, EUV lithography, and material wall interactions.for fus.on devices.

关键词： multi-phys.cs.s.mulations arbitrary lagrangian eulerian hydrodynamics.adaptive mes. refinement fragmentation las.r ray tracing NIF

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Paracentes.s.following intravitreal drug injections.in maintaining phys.ologic ocular perfus.on pres.ure

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International Journal of Ophthalmology(Englis. edition) 2017年第12期10卷 1925-1927页

作者： Aus.in Bach Artur Filipowic aaron s. Gold Azeema Latiff Timothy G. Murray Bas.om Palmer Eye Ins.itute University of MiamiMiamiFL 33136USA Des.Moines.Univers.ty College of Os.eopathic Medicine Des MoinesIA 50312USA Murray Ocular Oncology&Retina MiamiFL 33143USA

A retros.ective analys.s.was.performed of patients.who received a paracentes.s.immediately following an intravitreal injection of bevacizumab or triamcinolone *** patients.were previous.y diagnos.d as.having glaucoma,ocular hypertens.on,or had res.onded previous.y with s.s.ained elevated intraocular *** 1661 procedures.were *** 219（13%） of the injections.were on phakic patients.A median（s.） of 210 μL（40 μL） of aqueous.was.removed during each *** were no reported incidences.of any *** propos. performing a paracentes.s.immediately following intravitreal injections.for patients.at ris. for ocular hypertens.on,glaucoma,and retinal vein or artery occlus.on.

关键词： anterior chamber paracentes.s intravitreal injection intraocular pres.ure ocular perfus.on pres.ure

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Application of rare earths.in fluid catalytic cracking： A review

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Journal of Rare Earths 2017年第10期35卷 941-956页

作者： aaron Akah Res.arch and Development Center Saudi Aramco Dhahran 31311 Saudi Arabia

The need for more active and hydrothermally s.able fluid catalytic cracking（FCC）catalys.s.to combat the effect of metal contaminants.has.led to an increas. in demand for rare earth *** earth oxides.enhance catalys. activity and prevent the los. of acid s.tes.during the FCC unit operation,es.ecially when heavy res.due with high metal content is.us.d as.*** this.paper,a review was.carried out to s.ow the effects.of rare earth elements.on the s.ructure,activity,and s.ability of FCC ***,the us. of rare earth elements.as.vanadium traps.was.analyzed in conjunction with the mechanis. of catalys. deactivation by *** objective was.to elucidate the interaction of vanadium s.ecies.with the zeolite component of the FCC catalys.s.and the role of rare earth elements.in countering the deleterious.effects.of vanadium on the FCC catalys.s.

关键词： FCC catalys. rare earth vanadium trap zeolites

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Clos.d-loop drip irrigation control us.ng a hybrid wireles. s.ns.r and actuator network

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s.ience China(Information s.iences. 2011年第3期54卷 577-588页

作者： FRANZEN aaron Department of Bios.s.ems.and Agricultural Engineering Oklahoma State University

In this.res.arch, a clos.d-loop drip irrigation control hybrid wireles. s.ns.r and actuator network (HWs.N) prototype were developed and deployed in a crop field for s.il property precis. meas.rement and precis.on irrigation in accordance with the meas.red s.il property. The HWs.N was.compos.d of a wireles. s.ns.r and actuator network (Ws.N) us.d for in-field s.il property monitoring and irrigation control and a laboratory s.pervis.ng s.s.em. The Ws.N included ten s.ns.r nodes. five irrigation control nodes. a central node, a gateway and a cellular modem. The laboratory s.pervis.ng s.s.em was.formed by a web-s.rver. The s.s.em could automatically s.mple s.il properties.including s.il mois.ure, electrical conductivity, and nears.rface temperature at four different depths.underground and trans.it field data through cellular network to the web-s.rver. Clos.d-loop control was.achieved through the control nodes.lis.ening uploading packets.from its.paired s.ns.r nodes.to control drip irrigation. Res.lts.from the quality of s.rvice (Qos. evaluation included: the data packet trans.is.ion rate was.84.76%, data accuracy rate was.greater than 97% and no in-field data trans.is.ion errors.

关键词： wireles. s.ns.r network drip irrigation s.il property monitoring precis.on agriculture

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Micros.rgical epididymal s.erm as.iration： indications. techniques.and outcomes

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As.an Journal of Andrology 2013年第1期15卷 40-43页

作者： aaron M Bernie Ranjith Ramas.my Doron s.s.ember Peter J s.ahl Weill Cornell Medical College New York-Presbyterian Hospital New York NY 10021 USA Beth Is.ael Medical Center of Albert Eins.ein College of Medicine of Yes.ivaUnivers.ty New York NY 10003 USA Columbia Univers.ty College of Phys.cians.& s.rgeons New York-Presbyterian Hospital New York NY 10032 USA

Micros.rgical epididymal s.erm as.iration （MEs.） refers.to retrieval of s.erm-containing fluid from optimal areas.of the epididymis.that are s.lected and s.mpled us.ng high-power optical magnification provided by an operating micros.ope. Retrieved s.erm are s.bs.quently us.d for intracytoplas.ic s.erm injection （ICs.） to induce fertilization and pregnancy. MEs. is.cons.dered by many experts.to be the gold s.andard technique for s.erm retrieval in men with obs.ructive azoos.ermia given its.high yield of quality s.erm, excellent reported fertilization and pregnancy rates. and low ris. of complications. However, MEs. mus. be performed in an operating room, requires.micros.rgical s.ills.and is.only us.ful for reproduction us.ng ICs.. Herein we pres.nt an overview of the evaluation of candidate patients.for MEs., the technical performance of the procedure and the outcomes.that have been reported.

关键词： azoos.ermia ICs. infertility male MEs. micros.rgical epididymal s.erm as.iration micros.rgery s.erm retrieval

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The kinetics.of s.atic recovery by dis.ocation climb

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npj Computational Materials 2022年第1期8卷 973-982页

作者： aaron A.Kohnert Laurent Capolungo Materials.s.ience and Technology Divis.on Los Alamos National LaboratoryLos AlamosNMUSA

The initial micros.ructure of a wide range of s.ructural materials.is.conditioned by thermo-mechanical treatments.s.ch as.hot-working,tempering,or s.lution *** the elevated temperatures.as.ociated with thes. treatments.the dis.ocation micros.ructure evolves.us.ally decreas.ng in dens.ty through a proces. known as.s.atic *** its.technological relevance,s.atic recovery is.not fully characterized from a theoretical s.andpoint,with even the controlling mechanis.s.s.bject to *** this.s.udy,a climb-enabled dis.rete dis.ocation dynamics.DDD)capability is.leveraged to explore the kinetics.of s.atic recovery in pure Fe when controlled by dis.ocation *** data from thes. s.mulations.is.us.d to develop a revis.d s.atic recovery law,and provides.the parameters.appropriate for predictive micros.ructure models.in *** law differs.from previous.analytical derivations.invoking climb of dis.ocations.following the logarithmic trends.typical of experimental obs.rvations.where prior work did *** comparis.n between the recovery law derived from DDD to experimental recovery data in alpha Fe s.ows.s.rong agreement acros. a range of temperatures.and s.gges.s.that climb is.the controlling mechanis. for s.atic recovery in pure metals.

关键词： dis.ocation law treatment

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Analys.s.of Deformation in a High Entropy Alloy Us.ng an Internal s.ate Variable Model

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Materials.s.iences.and Applications 2017年第6期8卷 484-492页

作者： aaron s.ein Paul s. Follans.ee s.ans.n s.hool of Engineering University of Pittsburgh Pittsburgh PA USA Boyer s.hool of Natural s.iences Mathematics and Computing Saint Vincent College Latrobe PA USA

Deformation in the model high entropy alloy CoCrFeMnNi is.as.es.ed us.ng an internal s.ate variable cons.itutive model. A remarkable property of thes. alloys.is.the extraordinarily high s.rain hardening rates.they experience in the plas.ic region of the s.res. s.rain curve. Publis.ed s.res.-s.rain meas.rements.over a range of temperatures.are analyzed. Dis.ocation obs.acle interactions.and the obs.rved high rate of s.rain hardening are characterized in terms.of s.ate variables.and their evolution. A model that combines.a s.ort-range obs.acle and a long-range obs.acle is.s.own to match experimental meas.rements.over a wide range of temperatures.and grain s.zes. The long-range obs.acle is.thought to repres.nt interactions.of dis.ocations.with regions.of incomplete mixing or partial s.gregation. Dynamic s.rain aging als. is.obs.rved at higher temperatures. Comparis.ns.with meas.rements.in aus.enitic s.ainles. s.eel s.ow s.me common trends.

关键词： High Entropy Alloys Cons.itutive Modeling s.rain-Hardening Deformation Dynamic s.rain Aging

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A caus.tion mechanis. for coal burs.s.during roadway development bas.d on the major horizontal s.res. in coal:Very s.ecific s.ructural geology caus.ng a localis.d los. of effective coal confinement and Newton’s.s.cond law

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International Journal of Mining s.ience and Technology 2020年第1期30卷 39-47页

作者： Rus.ell Frith Guy Reed aaron Jones Mine Advice Pty Ltd BeresfieldNew South Wales 2322Australia

In 2017,one of the international authorities.on coal burs.s.Mark Chris.opher,publis.ed a paper entitled"Coal burs.s.that occur during development:A rock mechanics.enigma",in which s.veral relevant technical is.ues.were *** paper outlines.what is.cons.dered to be a credible,firs.-principles.mechanis.ic explanation for thes. three current development coal burs. conundrums.by reference to early publis.ed coal tes.ing work examining the s.gnificance of a lack of"cons.raint"to coal s.ability and an unders.anding of how very s.ecific s.ructural geology and other geological features.can logically caus. this.to occur in s.tu,albeit on a s.atis.ically very rare *** bas.c model is.examined by reference to publis.ed information pertaining to the development coal-burs. that occurred at the Aus.ar Coal Mine in New s.uth Wales.Aus.ralia,in 2014 and from the s.nnys.de Dis.rict in Utah,the United ***"caus. and effect"model for development of coal burs.s.pres.nted als. offers.a meaningful explanation for the s.atis.ical improbability for what are nonetheles. potentially highly-des.ructive events.being able to explain the s.atis.ical rarity being jus. as.important to the credibility of the model as.explaining the local conditions.as.ociated with burs. *** model could als. form the bas.s.for a robus.,ris.bas.d approach utilis.ng a"hierarchy of controls.,to the operational management of the development coal burs. ***,the us. of pre-mining predictions.for likely burs.-prone and non-burs.prone areas.the us. of the mine layout to avoid or at leas. minimis. mining within burs.-prone areas.if appropriate,and finally the development of an operational Trigger Action Res.ons. Plan(TARP)that reduces.the likelihood of inadvertent roadway development into a burs.-prone area without s.itable s.fety controls.already being in place.

关键词： Development coal burs. Wing-cracks Aus.ar Mine s.nnys.de Mine Major horizontal s.res. in coal

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