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Optimization of the Production Process of <i>Enzymatic Activity</i>of Lentinula edodes (Berk.) Pegler in Holocelulases

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Natural Resources 2014年第6期5卷 241-255页

作者： Juliane Andressa Chicatto Vitória Arend Castamann Cristiane Vieira Helm Lorena Benathar Ballod Tavares Brazilian Agricultural Research Corporation Research Center of Forestry—EMBRAPA Colombo Brazil Regional University of Blumenau—FURB Blumenau Brazil

Issues such as fossil fuels and oil supplies have stimulated the search for renewable alternatives such as biofuels. Agricultural crop residues represent an abundant renewable resource for the future of bioethanol. For it to be a viable alternative, the second-generation ethanol which ought to provide a net energy gain, environmental benefits, should be economically viable, and also be produced in large quantities without reducing food supplies. The current difficulty of lignocellulosic biofuel production is the hydrolysis of biomass into sugar. This is a work in which the white-rot Lentinula edodes fungus secretes substantial amounts of hydrolytic enzymes and is useful for degradation of lignocellulosic biomass which have not been described yet. The objective of this investigation was to evaluate the pH effect (5, 6 and 7), agitation (0, 100 rpm and 200 rpm) and also the cultivation time (6, 9 and 12 days). The culture medium was supplemented with agro-industrial residue and the EF 52 strain of the fungus Lentinula edodes was used as a processing agent. A factorial design 22 repeating the central point was performed. Submerged cultivation was conducted in a synthetic medium and was incubated at 25°C. The total protein content was determined as well as the activity of xylanase and cellulase (endoglucanase, exoglucanase and β-glucosidase). By Pareto diagram, the agitation and pH variables were significant for enzymatic activities. The highest enzyme expression occurred at pH values between 5.0 and 6.0 and above 100 rpm agitation. The exoglucanase was the enzyme which showed the highest activity in terms of cellulases, despite the cultivation time. Regarding the production of other enzymes and proteins, the most significant cultivation time was 12 days.

关键词： Bioethanol Cellulase Xylanase Enzymatic Hydrolysis Basidiomycetes

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Optimal Task Placement of a Serial Robot Manipulator for Manipulability and Mechanical Power Optimization

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Intelligent Information Management 2010年第9期2卷 512-525页

作者： Rogério Rodrigues dos Santos Valder Steffen Sezimária de Fátima Pereira Saramago 不详

Power consumption and accuracy are main aspects to be taken into account in the movement executed by high performance robots. The first aspect is important from the economical point of view, while the second is requested to satisfy technical specifications. Aiming at increasing the robot performance, a strategy that maximizes the manipulator accuracy and minimizes the mechanical power consumption is considered in this work. The end-effector is constrained to follow a predefined path during the optimal task positioning. The proposed strategy defines a relation between mechanical power and manipulability as a key element of the manipulator analysis, establishing a performance index for a rigid body transformation. This transformation is used to compute the optimal task positioning through the optimization of a multicriteria objective function. Numerical simulations regarding a serial robot manipulator demonstrate the viability of the proposed methodology.

关键词： Optimal Task Placement Optimal Robot Path Planning Multicriteria Optimization

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Effect of Long-Term in Vitro Sub-culturing on Quality Degeneration of Sweet Potato Varieties： Morpho- Anatomic Assessment and Simple Sequence Repeats Analysis

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Journal of Agricultural Science and Technology(A) 2014年第10期4卷 811-821页

作者： Mihiretu Cherinet Hundayehu Elsa Du Toit Sunette Laurie Martin Steyn ria Greyling Nokuthula Myeza Department of Plant Production and Soil Science University of Pretoria Lynwood Road Pretoria 0002 South Africa Agricultural Research Council-Roodeplaat Vegetable and Ornamental Plant Institute (ARC-VOPI) Private Bag X293 Pretoria0001 South Africa

The aim of this study was to assess the effect of long-term in vitro sub-culturing on the varietal degeneration of three sweet potato varieties, namely, Monate, Mokone and Ndou which were sub-cultured for 32, 23 and 12 generations, respectively. Each generation was cultured in a media which is made from 4.43 g/L Murashige and Skoog （MS）, 30 g/L sucrose and 2 g/L gelrite, respectively, and grown under 16 h light and 8 h dark photoperiod for 30 d. For each generation, 45 plantlets were acclimatized for two months in a glasshouse. Data on in vitro growth performance and 11 morphological characteristics during acclimatization were recorded. Early root and shoot formation was observed after the 27th and 21st sub-cultured generations of Monate and Mokone, respectively. During acclimatization, plantlets from the same variety showed differences in morphological traits such as leaf colour, abaxial leaf pigmentation, vine pigmentation, petiole pigmentation, leaf wrinkling and flowering. However, the rate of these morphological differences is random and irrespective to increase in sub-culturing. Therefore, to understand the genetic base of these morphological variability, two plantlets from each variety were subjected to genetic analysis by using five simple sequence repeat （SSR） primers （IB-242, IB-318, IB-255F, 1B-248 and IB-255）. Although SSR loci IB-255F and IB-318 could distinguish between the three varieties, there were no allelic polymorphisms detected in plantlets from the same varieties. Therefore, long-term sub-culturing do not leads to quality degeneration in the three sweet potato varieties.

关键词： Micro-propagation organogenesis sub-culture true-to-type morphology polymorphism.

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Evaluation of Taping in the Lymphatic System through Lymphoscintigraphy of Upper and Lower Limbs: A Case Study

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Health 2019年第5期11卷 527-534页

作者： Maria da Glória Karan Marquetti Anny Chi Cristiano Ferrari Siqueira Ian Fidelis Santos IUNIR AR Ponta Grossa Brasil The Physical Therapy Course CESUFOZ Foz do Igua&#231 u Brasil Medicine UNILA Foz do Igua&#231 u Brasil Faculty of Medicine Americas Sao Paulo Brazil

Introduction: Lymphatic taping has been used extensively in several areas of physical therapy, and has been prominent in the treatment of edema. Its results are considered empirical and no study reports its effect observed by a lymphocyte count study. Purpose: To verify the performance of lymphatic taping in the lymphatic system using lymphoscintigraphy examination. Methods: A 44-year-old male patient with no clinical signs of lymphatic alteration underwent lymphoscintigraphy examination prior to and after application of lymphatic taping in the upper and lower limbs on different days of placement to verify if the use of lymphatic taping may influence the lymph flow. Results: The result was an improvement in the lymphatic flow with application of lymphatic taping only in the lower limbs, where the taping acted only for 24 hours. There was a greater amount of radiopharmaceutical extracted in the feet of the patient, comparing pre- and post-application of taping. At the foot of the left lower limb, the absorption ratio doubled. There was also improvement in the right limb, but on a smaller scale. Conclusion: Through the evaluation of the lymphatic system by lymphoscintigraphy it can be observed that the values analyzed are relatively higher when the patient has lymphatic taping applied with both 10% and 20% of tension in the lower limbs.

关键词： Taping Lymphatic System Lymphoscintigraphy

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Anabolic steroid-induced cardiomyopathy underlying acute liver failure in a young bodybuilder

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World Journal of Gastroenterology 2009年第23期15卷 2920-2922页

作者： Miguel Bispo Ana Valente Rosário Maldonado Rui Palma Helena Glória Joo Nóbrega Paula Alexandrino Intensive Care Unit of Gastroenterology and Hepatology Santa Maria University Hospital 1069-166 Lisbon Portugal Department of Gastroenterology Egas Moniz Hospital 1349-019 Lisbon Portugal Coronary Care Unit Santa Maria University Hospital 1069-166 Lisbon Portugal

Heart failure may lead to subclinical circulatory disturbances and remain an unrecognized cause of ischemic liver injury. We present the case of a previously healthy 40-year-old bodybuilder, referred to our Intensive-Care Unit of Hepatology for treatment of severe acute liver failure, with the suspicion of toxic hepatitis associated with anabolic steroid abuse. Despite the absence of symptoms and signs of congestive heart failure at admission, an anabolic steroid-induced dilated cardiomyopathy with a large thrombus in both ventricles was found to be the underlying cause of the liver injury. Treatment for the initially unrecognized heart failure rapidly restored liver function to normal. To our knowledge, this is the f irst reported case of severe acute liver failure due to an unrecognized anabolic steroid-induced cardiomyopathy. Awareness of this unique presentation will allow for prompt treatment of this potentially fatal cause of liver failure.

关键词： Acute liver failure Anabolic steroids Bodybuilder Cardiomyopathy Hypoxic hepatitis

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飞灰颗粒的动电现象和表面特性

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电力情报 1990年第3期 89-96页

作者： Mal.,ria 赵英

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印尼留学生习得动词趋向补语“来/去”偏误分析

印尼留学生习得动词趋向补语“来/去”偏误分析

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作者：夏梦悠（ria RISKI MARSUKI）南昌大学

学位级别：硕士

现代汉语语法中的趋向补语是印尼学生最难掌握的汉语语法之一。在对外汉语教学研究中,对趋向补语的研究一直是一个热点,其中对印尼学生学习趋向补语情况的研究更是一个很重要的方面。本文,笔者将结合自身学习汉语的感受,对于印尼学生在... 详细信息

现代汉语语法中的趋向补语是印尼学生最难掌握的汉语语法之一。在对外汉语教学研究中,对趋向补语的研究一直是一个热点,其中对印尼学生学习趋向补语情况的研究更是一个很重要的方面。本文,笔者将结合自身学习汉语的感受,对于印尼学生在学习趋向补语过程中出现的一些问题进行解释。第一章为绪论、研究缘起、选择趋向补语“来/去”的原因、研究现状、对V+“来”和“去”本体的研究与汉语趋向补语的个案研究、汉语印尼语对比研究及其偏误分析、研究思路及研究办法。第二章介绍汉语趋向补语在印尼语中的对应表达、介绍汉语中趋向补语、汉语趋向补语的类型:简单趋向补语与复合趋向补语、汉语趋向补语的意义、汉语趋向补语与宾语的位置关系、汉语简单趋向补语在印尼语中的对应表达、以及汉语复合趋向补语在印尼语中的对应表达。第三章首先介绍笔者为印尼学生学习汉语趋向补语设计的一份调查问卷、外国留学生在学习汉语出现偏误的原因、问卷调查的对象、调查的办法、趋向补语调查问卷设计、最后分析印尼学生学习汉语趋向补语“来/去”的情况。第四章介绍印尼学生运用趋向补语的出现偏误的原因与教学建议。第一语言负迁移、学生个体因素和语言学习环境因素、学习者的个性差别、年龄因素与性格因素、教材因素等是印尼学生运用汉语趋向补语出现偏误的原因。最后提出教印尼学生学习方法。第五章结论。

关键词：汉语趋向补语印尼学生偏误分析

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Assessment of periportal fibrosis in Schistosomiasis mansoni patients by proton nuclear magnetic resonance-based metabonomics models

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World Journal of Hepatology 2022年第4期14卷 719-728页

作者： Milena Lima Rodrigues Tatiane Priscila Santos Rodrigues da Luz Caroline Louise Diniz Pereira Andrea Dória Batista Ana Lúcia Coutinho Domingues Ricardo Oliveira Silva Edmundo Pessoa Lopes Programa de Pós-Graduação em Medicina Tropical Centro de Ciências MédicasUniversidade Federal de PernambucoRecife 50670-901PernambucoBrazil Programa de Pós-Graduação em Química Centro de Ciências Exatas e da NaturezaUniversidade Federal de PernambucoRecife 50670-740PernambucoBrazil Hospital das Clínicas Departamento de Medicina ClínicaUniversidade Federal de PernambucoRecife 50670-901PernambucoBrazil

BACKGROUND The evaluation of periportal fibrosis(PPF)is essential for a prognostic assessment of patients with Schistosomiasis *** WHO Niamey Protocol defines patterns of fibrosis from abdominal ultrasonography,^(1)H-nuclear magnetic resonance(NMR)-based metabonomics has been employed to assess liver fibrosis in some *** To build 1H-NMR-based metabonomics models(MM)to discriminate mild from significant periportal PPF and identify differences in the metabolite *** A prospective cross-sectional study was performed on schistosomiasis patients at a University Hospital in Northeastern *** evaluated 41 serum samples from 10 patients with mild PPF(C Niamey pattern)and 31 patients with significant PPF(D/E/F Niamey patterns).MM were built using partial least squares-discriminant analysis(PLS-DA)and orthogonal projections to latent structures discriminant analysis(OPLS-DA)*** PLS-DA and OPLS-DA resulted in discrimination between mild and significant PPF groups with R2 and Q2 values of 0.80 and 0.38 and 0.72 and 0.42 for each model,*** OPLS-DA model presented accuracy,sensitivity,and specificity values of 92.7%,90.3%,and 100%to discriminate significant *** metabolites identified as responsible by discrimination were:Nacetylglucosamines,alanine,glycolaldehyde,carbohydrates,and *** MMs discriminated mild from significant PPF patterns in patients with Schistosomiasis mansoni through identification of differences in serum metabolites profiles.

关键词： Metabolomics Portal hypertension Schistosoma mansoni Biomarkers Neglected disease Nuclear magnetic resonance

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跨年夜突装

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财新周刊 2023年第2期 12-13页

作者： ria Novosti(图) 无不详视觉中国

当地时间2023年1月2日,顿涅茨克地区,俄罗斯紧急救援人员清除被炮击摧毁的废墟。俄罗斯国防部称,跨年夜期间,乌克兰武装力量对顿涅茨克马克耶夫卡地区的俄罗斯军人临时部署点进行了打击,截至1月4日,造成的死亡人数已上升至89人。俄军方... 详细信息

当地时间2023年1月2日,顿涅茨克地区,俄罗斯紧急救援人员清除被炮击摧毁的废墟。俄罗斯国防部称,跨年夜期间,乌克兰武装力量对顿涅茨克马克耶夫卡地区的俄罗斯军人临时部署点进行了打击,截至1月4日,造成的死亡人数已上升至89人。俄军方称,事后俄军对乌方进行了反击,摧毁乌方大量武器装备,并打死300余名乌军和外国雇佣兵。俄军方还称,调查显示,有土兵违反禁令违规使用手机,造成部署点地点泄露,将对相关负责人进行处罚。乌克兰武装部队总参谋部2日证实乌军对顿涅茨克地区马克耶夫卡的俄军进行了打击。乌克兰军方表示,马克耶夫卡袭击所导致的俄军死亡人数要比俄方公布的更高,约有400人死亡。

关键词：死亡人数顿涅茨克紧急救援打击俄军禁令乌克兰武器装备

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Heavy metal retention by soil mineral phases during a potential soil contamination by sewage sludge

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Chinese Journal Of Geochemistry 2006年第B8期25卷 194-195页

作者： Péter Sipos Tibor Németh Viktória Kováics Kis Ilona Mohai Institute for Geochemical Research Hungarian Academy of Sciences Budapest Hungary Research Institute for Technical Physics and Materials Science Hungarian Academy of Sciences Budapest Hungary Institute of Materials and Environmental Chemistry Chemical Research Center Hungarian Academy of Sciences Budapest Hungary

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