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Structural and optical properties of nano-spin coated sol-gel porous TiO_2 films

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Transactions of Nonferrous Metals Society of China 2012年第12期22卷 3003-3011页

作者： M.M.el-NAHASS M.H.ALI a.el-DENGLAWEY Physics Department Faculty of Education Ain Shams University Roxy Cairo 11757 Egypt Physics Department Faculty of Science Ain Shams University Cairo Egypt Physics Department Faculty of Science South Valley University Qena 83523 Egypt Phys Dep Fac of Appl Med Sci Turabah 21995 Taif University KSA

Three thicknesses of TiO2 films, 174, 195, and 229 nm, were deposited onto quartz substrates by sol–gel spin coating method. The as-deposited thin films were characterized by nano-crystallite with different sizes (19–46 nm) and relatively high porous structure. Optical constants were determined and showed the lowest refractive index of 1.66 for the as-prepared films that ever reported till now. Obtained results were discussed through current theoretical ideas.

关键词： TiO2 films optical properties nanostructure electron microscopy thin films spin coating technique

Intraoperative endoscopic retrograde cholangiopancreatography: A useful tool in the hands of the hepatobiliary surgeon

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World Journal of Gastroenterology 2015年第2期21卷 609-615页

作者： Ayman el Nakeeb Ahmad M Sultan Emad Hamdy Ehab el Hanafy Ehab Atef Tarek Salah a.med a.el geidie Tharwat Kandil Mohamed el Shobari Gamal el Ebidy Gastroenterology surgical center Mansoura UniversityDaqahlia 35516Egypt

AIM: To evaluate the efficacy of intraoperative endoscopic retrograde cholangio-pancreatography(ERCP) combined with laparoscopic cholecystectomy(LC) forpatients with gall bladder stones(GS) and common bile duct stones(CBDS).METHODS: Patients treated for GS with CBDS were included. LC and intraoperative transcystic cholangiogram(TCC) were performed in most of the cases. Intraoperative ERCP was done for cases with proven ***: Eighty patients who had GS with CBDS were included. LC was successful in all cases. Intraoperative TCC revealed passed CBD stones in 4 cases so intraoperative ERCP was performed only in 76 patients. Intraoperative ERCP showed dilated CBD with stones in 64 cases(84.2%) where removal of stones were successful; passed stones in 6 cases(7.9%); short lower end stricture with small stones present in two cases(2.6%) which were treated by removal of stones with stent insertion; long stricture lower 1/3 CBD in one case(1.3%) which was treated by open hepaticojejunostomy; and one case(1.3%) was proved to be ampullary carcinoma and whipple's operation was scheduled. CONCLUSION: The hepatobiliary surgeon should be trained on ERCP as the third hand to expand his field of therapeutic options.

关键词： Obstructive jaundice Endoscopic retrograde cholang

Post-endoscopic retrograde cholangiopancreatography pancreatitis: Risk factors and predictors of severity

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World Journal of Gastrointestinal Endoscopy 2016年第19期8卷 709-715页

作者： Ayman el Nakeeb Ehab el Hanafy Tarek Salah Ehab Atef Hosam Hamed Ahmad M Sultan Emad Hamdy Mohamed Said a.med a.el geidie Tharwat Kandil Mohamed el Shobari Gamal el Ebidy Gastroenterology Surgical Center Mansoura University

AIMTo detect risk factors for post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) and investigate the predictors of its severity. METHODSThis is a prospective cohort study of all patients who underwent ERCP. Pre-ERCP data, intraoperative data, and post-ERCP data were collected. RESULTSThe study population consisted of 996 patients. Their mean age at presentation was 58.42 (± 14.72) years, and there were 454 male and 442 female patients. Overall, PEP occurred in 102 (10.2%) patients of the study population; eighty (78.4%) cases were of mild to moderate degree, while severe pancreatitis occurred in 22 (21.6%) patients. No hospital mortality was reported for any of PEP patients during the study duration. Age less than 35 years (P = 0.001, OR = 0.035), narrower common bile duct (CBD) diameter (P = 0.0001) and increased number of pancreatic cannulations (P = 0.0001) were independent risk factors for the occurrence of PEP. CONCLUSIONPEP is the most frequent and devastating complication after ERCP. Age less than 35 years, narrower median CBD diameter and increased number of pancreatic cannulations are independent risk factors for the occurrence of PEP. Patients with these risk factors are candidates for prophylactic and preventive measures against PEP.

关键词： Pancreatitis Obstructive jaundice Endoscopic retrograde cholangiopancreatography

内镜下注射聚糖酐/透明质酸共聚物治疗小儿膀胱输尿管返流

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临床外科杂志 2005年第6期13卷 369-370页

作者：余玲 a.el Ghoneimi Y.Aigrain 上海市儿童医院小儿外科巴黎ROBERT DEBRE医院小儿泌尿外科

目的介绍巴黎ROBERT DEBRE医院的小儿外科应用在膀胱镜下输尿管下端开口处注射聚糖酐/透明质酸共聚物治疗(DEFLUX)治疗小儿膀胱输尿管返流的经验.方法选取从2001～2003年,ROBERT DEBRE 医院(巴黎)小儿外科治疗的57个患儿,其中男11例,女4... 详细信息

目的介绍巴黎ROBERT DEBRE医院的小儿外科应用在膀胱镜下输尿管下端开口处注射聚糖酐/透明质酸共聚物治疗(DEFLUX)治疗小儿膀胱输尿管返流的经验.方法选取从2001～2003年,ROBERT DEBRE 医院(巴黎)小儿外科治疗的57个患儿,其中男11例,女46例.平均年龄71个月.患者有急性肾盂肾炎(APN)史,有药物治疗史,并有不同程度的膀胱输尿管返流.共80侧返流的输尿管,行膀胱镜下输尿管开口处注射DEFLUX.结果治疗的患儿术后1年随访.未发现明显的并发症.被治愈的输尿管共58侧(72.5%),1年内未有尿路感染的45例(78.9%).Ⅰ度返流治愈率82.5%.Ⅱ度返流治愈率75.9%.Ⅲ度返流治愈率68.7%.Ⅳ度返流治愈率0.结论在膀胱镜下输尿管下端注射DEFLUX来治疗小儿膀胱输尿管返流,从而控制因膀胱输尿管返流引起的尿路感染是有效的,且安全和简单.

关键词：内窥镜术膀胱输尿管返流儿童

HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro

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World Journal of Gastroenterology 2006年第30期12卷 4836-4842页

作者： Mostafa K el-Awady Ashraf A Tabll Yasmine S el-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader el Din el-Rashdy M Redwan Maha.el-Demellawy Moataza H Omran Wael T el-Garf Said A Goueli Department of Biomedical Technology National Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of medicinal Chemistry Na- tional Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of Microbiology and Immunology Faculty of Pharmacy Cairo University Cairo Egypt Genetic Engineering and Biotechnology Research Institute New Borg El-Arab City Alexandria Egypt Department of Pathology School of medicine University of Wisconsin Madison WI United States

AIM： TO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. METHODS： HepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect na＇ive cells. The presence of minus （antisense） RNA strand, and the detection of core and E1 antigens in cells were examined by RT-PCR and immunological techniques （flow cytometry and Western blot） respectively. RESULTS： The intra.ellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. The fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intra.ellular HCV antigen expression using in house made polyclonal antibodies （anti-core, and anti-E1）. Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kDa in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells. CONCLUSION： HepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. These cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.

关键词： Hepatitis C virus In vitro propagation Genomic replication Gene expression HepG2 cells

Possible role of intestinal stem cells in the pathophysiology of irritable bowel syndrome

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World Journal of Gastroenterology 2020年第13期26卷 1427-1438页

作者： Magdy el-Salhy Section for Gastroenterology Department of MedicineStord HospitalStord 5409Norway Department of Clinical medicine University of BergenBergen 5021Norway

The pathophysiology of irritable bowel syndrome(IBS)is not completely ***,several factors are known to play a role in pathophysiology of IBS such as genetics,diet,gut microbiota,gut endocrine cells,stress and low-grade *** the pathophysiology of IBS may open the way for new treatment *** density of intestinal stem cells and low differentiation toward enteroendocrine cells has been reported recently in patients with *** abnormalities are believed to be the cause of the low density of enteroendocrine cells seen in patients with *** cells regulate gastrointestinal motility,secretion,absorption and visceral *** dysmotility,abnormal absorption/secretion and visceral hypersensitivity are all seen in patients with IBS and haven been attributed to the low density the intestinal enteroendocrine cells in these *** present review conducted a literature search in medline(Pub med)covering the last ten years until November 2019,where articles in English were *** about the intestinal stem cells and their possible role in the pathophysiology of IBS are discussed in the present *** present review discusses the assumption that intestinal stem cells play a central role in the pathophysiology of IBS and that the other factors known to contribute to the pathophysiology of IBS such as genetics,diet gut microbiota,stress,and lowgrade inflammation exert their effects through affecting the intestinal stem *** reports further the data that support this assumption on genetics,diet,gut microbiota,stress with depletion of glutamine,and inflammation.

关键词： Diet Gut enteroendocrine cells Gut microbiota Low grade inflammation Stress

The Impact of the Techniques and Tactics Appropriate by the Athletes in Phase Triple Jump and Their Relationships with the Finale Results

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Journal of Sports Science 2015年第4期3卷 179-185页

作者： Zerf Moha.med Mokkedes Moulay Idriss Bengoua Ali Bendahmane med Nasreddin Guebli Abd-el-Kader Physical Education Institute Laboratory OPAPS University Abdel Hamid lbn Badis Mostaganem Mostaganem 27000 Algeria

The purposes of this study were to describe and compare the techniques used by elite world triple jumper and the Algerian elite team to determine the impact of distribution modality phases allowing the elite to exceeds 17-meter. Our subjects were two world elite 2009 and two Algerian national elite 2013. In the lack of new technology Modem measuring instruments kinematics or kinetics, our study based in measure of the Phase distribution ratio as measure of Stride length Jump distance. That literature review two schools, the Russian technique （which emphasizes the hop phase） and the Polish technique （which emphasizes the jump phase）. Our aims for this study are： Are there any statistically significant differences between the results of the samples in the distance achieved？ Are there any statistically significant differences between practical Ratio relative distances （world 2009-national 2013）？ Are there any statistically significant differences between Stride length phase distances （world 2009-national 2013）？ For that, we have chosen the analysis of variance and correlation of the distances achieved in each of the phases with the official distance of jumper and ＂T＂ student to compare implementation distribution of phases （hop, step and jump） with the results accuses. Based on the practices and weaknesses of elites in practice, we confirm：（1） All the samples practice the Russian technique as model in the distribution of the phase ratio; （2） The problem of our elites is in the hop phase distribution and its relationship with other phases; （3） Improve achieve horizontal vertical velocity in hop phase as solution.

关键词： The ratio phase techniques tactics appropriate triple jump.

Shadow Volume in Real-Time Rendering

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Computer Technology and Application 2011年第8期2卷 638-643页

作者： Abd el Moumene Zerari Mohamed Chaouki Babahenini Department of Computer Science LESIA Laboratory University Med Khider Biskra Biskra 07000 Algeria

This paper presents an optimization of shadow volume algorithm, which allow a rendering in real-time. This technique is based on previous works which makes it possible to obtain shadows in real-time, although the calculation of the silhouette requires a pretreatment of the geometry implemented on the CPU （Central Processing Unit）. By using last version of the GPU （Graphic Processing Unit）, the authors propose to implement the calculation of the silhouette on the GPU by using Geometry Shader. The authors present the step which made it possible to lead to a concrete implementation of this algorithm, the modifications which were made, as well as a comparative study of results, followed by a discussion of these results and choices of implementation.

关键词： Shadow volumes silhouette GPU (graphic processing unit) real-time shaders.

Antibody to el peptide of hepatitis C virus genotype 4 inhibits virus binding and entry to HepG2 cells in vitro

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World Journal of Gastroenterology 2006年第16期12卷 2530-2535页

作者： Mostafa K el-Awady Ashraf A Tabll Khaled Atef Samar S Yousef Moataza H Omran Yasmin EI-Abd Noha G Bader-eldin Ahmad M Salem Samir F Zohny Wael T EI-Garf Department of Biomedical Technology National Research CenterCairoEgypt Department of Biochemistry Faculty of ScienceAin Shams UniversityCairoEgypt

AIM： To analyze the neutralizing activity of antibodies against E1 region of hepatitis C virus （HCV）. Specific polydonal antibody was raised via immunization of New Zealand rabbits with a synthetic peptide that had been derived from the E1 region of HCV and was shown to be highly conserved among HCV published genotypes. METHODS： Hyper-immune HCV E1 antibodies were incubated over night at 4 ℃ with serum samples positive for HCV RNA, with viral loads ranging from 615 to 3.2 million IU/mL. Treated sera were incubated with HepG2 cells for 90 min. Blocking of viral binding and entry into cells by anti E1 antibody were tested by means of RTPCR and flow cytometry. RESULTS： Direct immunostaining using FITC conjugated E1 antibody followed by Flow cytometric analysis showed reduced mean fluorescence intensity in samples pre-incubated with E1 antibody compared with untreated samples. Furthermore, 13 out of 18 positive sera （72%） showed complete inhibition of infectivity as detected by RT-PCR. CONCLUSION： In house produced E1 antibody, blocks binding and entry of HCV virion infection to target cells suggesting the involvement of this epitope in virus binding and entry. Isolation of these antibodies that block virus attachment to human cells are useful as therapeutic reagents.

关键词： Flow cytometry Hepatitis C virus E1 envelope Therapeutic antibodies Direct immuno-fluorescence HepG2 cells