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抗猪囊虫单克隆抗体对脑囊虫病的诊断价值

宁夏医学院学报 2003年第5期25卷 320-321,325页

作者：孙涛杨宝珍潘月英王利新宁夏医学院附属医院银川750004

目的 :评价用自制的抗猪囊虫单克隆抗体建立I-ELIsA检测方法应用于临床脑囊虫病的诊断价值。方法 :用低温无水乙醇沉淀法分离纯化单抗IgG(腹水抗体效价 1∶32 0 0 ) ;用改良的过碘酸钠法制备辣根过氧化物酶标单抗 ;用抑制性酶联免疫吸附... 详细信息

目的 :评价用自制的抗猪囊虫单克隆抗体建立I-ELIsA检测方法应用于临床脑囊虫病的诊断价值。方法 :用低温无水乙醇沉淀法分离纯化单抗IgG(腹水抗体效价 1∶32 0 0 ) ;用改良的过碘酸钠法制备辣根过氧化物酶标单抗 ;用抑制性酶联免疫吸附法 (I-ELIsA)检测患者脑脊液和血清标本中囊虫特异性抗体。结果 :检测 6 3例确诊为脑囊虫病的患者脑脊液 ,抗体阳性 6 1例 ,阳性率为 97% ;血清 6 0例 ,抗体阳性 5 3例 ,阳性率为 88% ;其他颅内感染疾病患者脑脊液标本 2 4 0例 ,血清标本 2 4 0例 ,肝肺包虫病患者血清 10例 ,正常脑脊液 2 5例 (阑尾炎手术腰麻患者 ) ,健康志愿者血清 32例 ,检测结果均为阴性。结论 :抗囊虫抗原的单克隆抗体用常规鼠源B细胞杂交瘤技术获得 ,该单克隆抗体具有相当高的特殊性和检出率。

关键词：抗猪囊虫单克隆抗体脑囊虫病诊断检测脑脊液血清

sTUDIEs ON THE BIODEGRADATION MECHANIsM OF β-TRICAICIUM PHOsPHATE CERAMICs

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Journal of Wuhan University of Technology(Materials science) 1997年第4期12卷 1-6页

作者：阎玉华 Wuhan University of Technology China Res. Ctr. of med. Mat./Engineering Wuhan University of Technology Wuhan 430070 China Xiehe Hospital Tongji Medical University China 203 Hospital Chinese People's Liberation Army China

In order to clarify the biodegradation mecha-nism of?-tricalcium phosphate (β-TCP) ceramics, In vitro and In vivo experiments have been carried out. Nanometric microelectrodes were applied to measure the pH values in-side and outside phagocytes which were cultured for 72 hours respectively in (β-TCP-bearing and (β-TCP-free medi-um. Radioactive isotope 45 Ca was used to trace the distribu-tion and metabolism process of degraded products. We found that the degradation of (β-TCP ceramics was due to the dis-solving process in body fluid and the degrading process me-diated by cells. After (β-TCP ceramics labelled by 45 Ca were implanted into rabbits,radioactive 45 Ca was tested in blood, faece and organs such as heart, kidney and stomach etc. It was also found in the nearby new bone. so,we think a small part of the degraded products enter the animal's blood and take part in the metabolism of body . Others are reused to form new bone. The reports from clinical application also gave us strong support.

关键词： β-TCP biodegradation metabolism

日本乙型脑炎病毒单克隆抗体介导ADCC效应的研究

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细胞与分子免疫学杂志 1986年第Z1期 40-46页

作者：张明杰汪美先姜绍谆马文煜第四军医大学微生物学教研室

以日本乙型脑炎病毒（JEV）感染的BHK2,细胞为靶细胞,小鼠脾细胞为效应细胞,测定了6株日本乙型脑炎病毒单克隆抗体（JEV-McAb）介导ADCC效应的活性。结果表明,2H4、2F2、nG2、mG9等种特异性及属特异性McAb无介导51Cr释放的活性,而mC3及... 详细信息

以日本乙型脑炎病毒（JEV）感染的BHK2,细胞为靶细胞,小鼠脾细胞为效应细胞,测定了6株日本乙型脑炎病毒单克隆抗体（JEV-McAb）介导ADCC效应的活性。结果表明,2H4、2F2、nG2、mG9等种特异性及属特异性McAb无介导51Cr释放的活性,而mC3及2D2两株亚组特异性McAb可介导ADCC效应;但mC3和2D2介导ADCC效应的活性也不相同。JEV-McAb的ADCC活性与血凝抑制（HI）、中和（NT）等功能之间无明显的关系,它是JEV-McAb的又一免疫学特征。小鼠感染JEV后,其脾细胞的ADCC活性下降,这可能与JEV的致病性有关。在JEV感染中,ADCC效应具有一定的保护功能。

关键词：日本乙型脑炎病毒单克隆抗体 ADCC效应

Endosonography guided management of pancreatic fluid collections

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World Journal of Gastroenterology 2015年第41期21卷 11842-11853页

作者： Andreas s Vilmann John Menachery shou-Jiang Tang Indu srinivasan Peter Vilmann Department of surgical Gastroenterology Copenhagen University Hospital Herlev Department of Gastroenterology Rajagiri Hospital Aluva Division of Digestive Diseases Department of Medicine University of Mississippi Medical Center

The revised Atlanta classification of acute pancreatitis was adopted by international consensus, and is based on actual local and systemic determinants of disease severity. The local determinant is pancreatic necrosis(sterile or infected), and the systemic determinant is organ failure. Local complications of pancreatitis can include acute peri-pancreatic fluid collection, acute necrotic collection, pseudocyst formation, and walledoff necrosis. Interventional endoscopic ultrasound(EUs) has been increasing utilized in managing these local complications. After performing a Pub med search, the authors manually applied pre-defined inclusion criteria or a filter to identify publications relevant to EUs and pancreatic collections(PFCs). The authors then reviewed the utility, efficacy, and risks associated with using therapeutic EUs and involved EUs devices in treating PFCs. Due to the development and regulatory approval of improved and novel endoscopic devices specifically designed for transmural drainage of fluid and necrotic debris(access and patency devices), the authors predict continuing evolution in the management of PFCs. We believe that EUs will become an indispensable part of procedures used to diagnose PFCs and perform image-guided interventions. After draining a PFC, the amount of tissue necrosis is the most important predictor of a successful outcome. Hence, it seems logical to classify these collections based on their percentage of necrotic component or debris present when viewed by imaging methods or EUs. Finally, the authors propose an algorithm for managing fluid collections based on their size, location, associated symptoms, internal echogenic patterns, and content.

关键词： Endoscopic ultrasound Drainage Pancreatic fluid co

实验性非压迫腰椎间盘髓核突出大鼠模型的建立

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宁夏医学院学报 2003年第3期25卷 167-169,172页

作者：陆志东金群华陈志荣宁夏医学院附属医院骨科银川750004

目的 :设计一种新的非压迫性腰椎间盘髓核突出动物模型。方法 :选择 7只sprague -Dawley(sD)雄性大鼠 ,将自身尾椎髓核混悬液注射到腰椎硬膜外腔建立动物模型。测量术前、术后 3、5、7、9d大鼠后肢机械刺激缩爪阈值。结果 :在没有明显... 详细信息

目的 :设计一种新的非压迫性腰椎间盘髓核突出动物模型。方法 :选择 7只sprague -Dawley(sD)雄性大鼠 ,将自身尾椎髓核混悬液注射到腰椎硬膜外腔建立动物模型。测量术前、术后 3、5、7、9d大鼠后肢机械刺激缩爪阈值。结果 :在没有明显机械压迫下 ,造模后大鼠出现明显的痛觉过敏反应。结论 :本模型简单、可靠、费用低廉 ,为进一步研究腰椎间盘突出症和根性神经痛提供了理想的动物模型。

关键词：腰椎间盘髓核突出痛觉过敏动物模型根性神经痛

The　Importance　of　scientific　Research　on　Fu-Fang　Chinese　medicine　Prescriptions

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Chinese Journal of Integrative medicine 1997年第2期3卷 155-156页

作者： $$$$ Kelvin Chan(school of Pharmacy & Chemistry, Liverpool John Moore’s University, UK.) ed.surg.med.tohoku.ac.jp

Ｔｈｅ　Ｉｍｐｏｒｔａｎｃｅ　ｏｆ　Ｓｃｉｅｎｔｉｆｉｃ　Ｒｅｓｅａｒｃｈ　ｏｎ　Ｆｕ－Ｆａｎｇ　Ｃｈｉｎｅｓｅ　Ｍｅｄｉｃｉｎｅ　ＰｒｅｓｃｒｉｐｔｉｏｎｓＫｅｌｖｉｎＣｈａｎ（ＳｃｈｏｏｌｏｆＰｈａｒｍａｃｙ＆Ｃ... 详细信息

Ｔｈｅ　Ｉｍｐｏｒｔａｎｃｅ　ｏｆ　Ｓｃｉｅｎｔｉｆｉｃ　Ｒｅｓｅａｒｃｈ　ｏｎ　Ｆｕ－Ｆａｎｇ　Ｃｈｉｎｅｓｅ　Ｍｅｄｉｃｉｎｅ　ＰｒｅｓｃｒｉｐｔｉｏｎｓＫｅｌｖｉｎＣｈａｎ（ＳｃｈｏｏｌｏｆＰｈａｒｍａｃｙ＆Ｃｈｅｍｉｓｔｒｙ，Ｌｉｖｅｒｐｏｏ...

关键词： Research Fu-Fang Importance scientific Chinese

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骨桥蛋白在胰腺癌中的表达及其意义

宁夏医科大学学报 2009年第3期31卷 341-342,345,F0004页

作者：李西梅杨少奇黄李雅阮继刚杨力宁夏医科大学附属医院消化科银川750004

目的探讨骨桥蛋白(OPN)在胰腺癌组织中表达的临床意义。方法应用免疫组织化学(s-P)方法检测OPN在50例胰腺癌、30例癌旁组织中的表达,并分析其与胰腺癌临床病理特征的关系。结果50例胰腺癌标本中32例OPN表达阳性,阳性表达率为64.0%,30例... 详细信息

目的探讨骨桥蛋白(OPN)在胰腺癌组织中表达的临床意义。方法应用免疫组织化学(s-P)方法检测OPN在50例胰腺癌、30例癌旁组织中的表达,并分析其与胰腺癌临床病理特征的关系。结果50例胰腺癌标本中32例OPN表达阳性,阳性表达率为64.0%,30例胰腺癌旁组织中6例OPN表达阳性,阳性表达率为20.0%,OPN在胰腺癌组织中的表达明显高于癌旁组织(P<0.05)。OPN的表达与肿瘤的分化程度、TNM分期及淋巴结转移有关(P<0.05),与肿瘤直径、性别和年龄无明显关系。结论OPN在胰腺癌中呈高表达,其表达与胰腺癌侵袭转移密切相关。

关键词：骨桥蛋白胰腺癌免疫组织化学

Biomarkers in triple negative breast cancer:A review

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World Journal of Clinical Oncology 2015年第6期6卷 252-263页

作者： Budhi s Yadav Priyanka Chanana swaty Jhamb Department of Radiotherapy PGIMER Department of Pharmacology Panjab University Dr Hs Judge Institute of Dental sciences Panjab University

Breast cancer is an intrinsically heterogeneous disease. In the world about 1 million cases of breast cancer are diagnosed annually and more than 170000 are triplenegative. Characteristic feature of triple negative breast cancer(TNBC) is that it lacks expression of oestrogen,progesterone and human epidermal growth factor receptor-2/neu receptors. They comprise 15%-20% of all breast cancers. We did a systematic review of Pub med and conference databases to identify studies published on biomarkers in TNBC. We included studies with biomarkers including: Epidermal growth factor receptor,vascular endothelial growth factor,c-Myc,C-kit and basal cytokeratins,Poly(ADP-ribose) polymerase-1,p53,tyrosinase kinases,m-TOR,heat and shock proteins and TOP-2A in TNBC. We also looked for studies published on synthetic lethality and inhibition of angiogenesis,growth,and survival pathways. TNBC is a complex disease subtype with many subclasses. Majority TNBC have a basal-like molecular phenotype by gene expression profiling. Their clinical and pathologic features overlap with hereditary BRCA1 related breast cancers. Management of these tumours is a challenge to the clinician because of its aggressive behaviour,poor outcome,and absence of targeted therapies. As the complexity of this disease is being simplified over time new targets are also being discovered for the treatment of this disease. There are many biomarkers in TNBC being used in clinical practice. Biomarkers may be useful as prognostic or predictive indicators as well as suggest possible targets for novel therapies. Many targeted agents are being studied for treatment of TNBC.

关键词： Triple negative breast cancer Epidermal growth factor receptor Vascular endothelial growth factor p53 Cyclin

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1岁儿童的胰腺黏液性囊腺瘤1例

世界核心医学期刊文摘（儿科学分册） 2005年第9期1卷 26-27页

作者： Lau s.T. Kim s.s. Lee s.L. schallerR.T. 郭战宏 Children' s Hosp. and Reg. med. Ctr. Seattle WA United States Dr.

Mucinous cystadenomas of the pancreas are uncommon lesions in adults and even more rare in children. This report presents a case of a pancreatic mucinous cystadenoma in a 1-year-old boy and offers a review of the lite... 详细信息

关键词：黏液性囊腺瘤手术适应证