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Chinese Chemical Letters 1995年第8期6卷 669-672页

作者： Liu, XH Wang, L Guan, BH Kong, m Zhang, XQ Tao, PZ Chen, HS Chinese Acad med Sci Inst Mat Med Beijing 100050 Peoples R China Beijing Union med Coll Beijing 100050 Peoples R China Chinese Acad med Sci Inst Med Biotechnol Beijing 100050 Peoples R China

We have synthesized the title compounds(1)and(2).The structure of regioisomer(4).a new acyclic nucleotide,was determined by spectroscopic methods (1H-NmR.13C-NmR.13C-1H HmBC).A by-product(7)was also isolated and ident...

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HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro

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World Journal of Gastroenterology 2006年第30期12卷 4836-4842页

作者： mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd mahmoud m Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy m Redwan maha El-Demellawy moataza H Omran Wael T El-Garf Said A Goueli Department of Biomedical Technology National Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of medicinal Chemistry Na- tional Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of microbiology and Immunology Faculty of Pharmacy Cairo University Cairo Egypt Genetic Engineering and Biotechnology Research Institute New Borg El-Arab City Alexandria Egypt Department of Pathology School of medicine University of Wisconsin Madison WI United States

AIm： TO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. mETHODS： HepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect na＇ive cells. The presence of minus （antisense） RNA strand, and the detection of core and E1 antigens in cells were examined by RT-PCR and immunological techniques （flow cytometry and Western blot） respectively. RESULTS： The intracellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. The fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intracellular HCV antigen expression using in house made polyclonal antibodies （anti-core, and anti-E1）. Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kda in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells. CONCLUSION： HepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. These cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.

关键词： Hepatitis C virus In vitro propagation Genomic replication Gene expression HepG2 cells

依那普利与氯沙坦对1型糖尿病患者肾脏及视网膜病变的影响

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中国中西医结合肾病杂志 2009年第8期10卷 673-673页

作者： mauer m N Engl J med 张益民（摘）李幼姬（校）不详中山大学附属第二医院肾内科 510120 中山大学附属第一医院肾脏病研究所 510080

肾脏及视网膜病变是1型糖尿病最重要的并发症,糖尿病患者一旦被检测到白蛋白尿即意味着肾脏结构发生了病理改变.已往的研究表明肾素-血管紧张素系统(RAS)阻滞剂较其他类型降压药可更有效地延缓已发生蛋白尿、糖尿病、肾小球滤过率(GFR)... 详细信息

肾脏及视网膜病变是1型糖尿病最重要的并发症,糖尿病患者一旦被检测到白蛋白尿即意味着肾脏结构发生了病理改变.已往的研究表明肾素-血管紧张素系统(RAS)阻滞剂较其他类型降压药可更有效地延缓已发生蛋白尿、糖尿病、肾小球滤过率(GFR)下降的肾脏病进展,并可降低糖尿病肾病患者的蛋白尿.最近的DI-RECT研究报告了RAS阻滞剂可降低血压正常且无糖尿病肾病、蛋白尿正常的1型糖尿病患者视网膜病变的发生率,但是对已经出现轻-中度视网膜病变的患者无效.我们的研究旨在评价RAS阻滞剂(包括ACEI和ARB)对血压、蛋白尿均正常的1型糖尿病患者肾脏与视网膜结构的影响.

关键词：视网膜病变糖尿病患肾脏结构依那普利肾素-血管紧张素系统糖尿病肾病患者氯沙坦 RAS阻滞剂

Treatment of Visceral Pain Associated with Irritable Bowel Syndrome using Acupuncture: mechanism of Action

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World Journal of Traditional Chinese medicine 2019年第4期5卷 181-186页

作者： Aleksandar Zivaljevic Bin Shi Elisa m.S.Tam Vahideh Toossi New Zealand College of Chinese medicine

Irritable bowel syndrome(IBS)is a relatively common condition characterized by abdominal pain,among other symptoms,that significantly impacts the quality of life of IBS *** treatment of IBS results in limited success,and the focus is placed on relieving patients of some of the symptoms,visceral pain in *** is commonly used as a treatment modality of ***,the debate on whether acupuncture can be effectively used for this purpose is *** this work,we critically review the available literature to establish a potential mechanism of action in treating visceral pain in IBS using *** sources used are Google Scholar,EBSCO,Cochrane Library and Pub med as well as Chinese database *** keywords used in the literature search are"acupuncture,""IBS,""Irritable Bowel Syndrome,"and"visceral pain."We find that the literature strongly indicates that acupuncture,by stimulating points located on the dermatomes proximal to the spinal level of the area where the sympathetic outflow of the particular gut area affected by the pain is,can interfere with the efferent signal that transports information about the noxious stimuli and interrupt the"connectivity"between the gut and brain,and as a final result,reduce or stop IBS *** findings justify that clinical trials are conducted to test the utility of acupuncture in treating abdominal visceral pain in IBS.

关键词： Acupuncture irritable bowel syndrome visceral pain

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膝关节前髁解剖的性别差异

中华骨科杂志 2010年第3期30卷 I0018-I0023页

作者： Thomas K. Fehring, mD Susan m. Odum, med CCRC, Josh Hughes, BS Bryan D. Springer, mD Walter B. Beaver Jr., mD 张伟滨(译) 王青禾(译) Investigation performed at the OrthoCarolina Hip and Knee Center Charlotte North Carolina 上海瑞金医院骨科 200025

背景：膝关节前髁解剖形态存在明显性别差异的观点已使存全膝关节假体设计时专门为女性患者设计了薄型前髁假体。我们通过对自己的患者进行观察，发现前髁的解剖形态变异很大，不仅取决于患者的性别，还取决于患者的身材、身高和人种。... 详细信息

背景：膝关节前髁解剖形态存在明显性别差异的观点已使存全膝关节假体设计时专门为女性患者设计了薄型前髁假体。我们通过对自己的患者进行观察，发现前髁的解剖形态变异很大，不仅取决于患者的性别，还取决于患者的身材、身高和人种。鉴于所发现的膝关节前髁解剖存在较大变异，我们试图明确是否真正存在前髁解剖的性别差异。方法：我们对212例随机挑选患者的mRI（男112例，女100例）进行评估。前髁定义为膝关节间隙以上10mm的股骨前侧皮质前方的骨性区域。前髁的内、外侧高度直接从mRI两个层面的标记值读出，以毫米为单位。经计算得出的髁高横径比用来确定患者的身材对前髁的大小是否有影响。

关键词：解剖形态性别差异全膝关节女性患者假体设计形态变异膝关节间隙 mRI

Duodenal gangliocytic paraganglioma with lymph node metastases: a case report and comparative review of 31 cases

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World Journal of Clinical Cases 2017年第6期5卷 222-233页

作者： Sahara J Cathcart Aaron R Sasson Jessica A Kozel Jennifer m Oliveto Quan P Ly Department of Pathology and microbiology 985900 Nebraska Medical Center Department of Surgery Division of Surgical Oncology Stony Brook Medicine midwest Pathology Associates LLC Department of Radiology University of Nebraska Medical Center Department of Surgery Division of Surgical Oncology University of Nebraska Medical Center

Gangliocytic paraganglioma(GP) is a rare tumor of uncertain origin most often located in the second portion of the duodenum. It is composed of three cellular components: Epithelioid endocrine cells, spindlelike/sustentacular cells, and ganglion-like cells. While this tumor most often behaves in a benign manner, cases with metastasis are reported. We describe the case of a 62-year-old male with a periampullary GP with metastases to two regional lymph nodes who was successfully treated with pancreaticoduodenectomy. Using Pub med, EmBASE, EBSCOhost medLINE and CINAHL, and Google Scholar, we searched the literature for cases of GP with regional lymph node metastasis and evaluated the varying presentations, diagnostic workup, and disease management of identified cases. Thirty-one cases of GP with metastasis were compiled(30 with at least lymph node metastases and one with only distant metastasis to bone), with age at diagnosis ranging from 16 to 74 years. Ratio of males to females was 19:12. The most common presenting symptoms were abdominal pain(55%) and gastrointestinal bleeding or sequelae(42%). Twenty-five patients underwent pancreaticoduodenectomy. Five patients were treated with local resection alone. One patient died secondary to metastatic disease, and one died secondary to perioperative decompensation. The remainder did well, with no evidence of disease at follow-up from the most recent procedure(except two in which residual disease was deliberately left behind). Of the 26 cases with sufficient histological description, 16 described a primary tumor that infiltrated deep to the submucosa, and 3 described lymphovascular invasion. Of the specific immunohistochemistry staining patterns studied, synaptophysin(SYN) stained all epithelioid endocrine cells(18/18). Neuron specific enolase(NSE) and SYN stained most ganglion-like cells(7/8 and 13/18 respectively), and S-100 stained all spindle-like/sustentacular cells(21/21). Our literature review of published cases of GP

关键词： Gangliocytic paraganglioma metastases duodenum lymph node dissection pancreaticoduodenectomy

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国内外海水淡化发展现状

膜科学与技术 2021年第4期41卷 170-176页

作者：宋瀚文宋达张辉付赫彭昌盛中国海洋大学环境科学与工程学院青岛266100 自然资源部天津海水淡化与综合利用研究所天津300192

海水淡化作为一种水资源开源增量手段,已经成为很多国家和地区解决水资源危机的重要手段.到2018年,全球海水淡化产能为6.2×10^(7)m^(3)/d,采用的技术主要是RO、mSF、med,分别占全球总产能的54%、31%和10%,占淡化厂总数量的71%、11%和1... 详细信息

海水淡化作为一种水资源开源增量手段,已经成为很多国家和地区解决水资源危机的重要手段.到2018年,全球海水淡化产能为6.2×10^(7)m^(3)/d,采用的技术主要是RO、mSF、med,分别占全球总产能的54%、31%和10%,占淡化厂总数量的71%、11%和14%.其中mSF和med技术主要分布在中东和北非地区,其他地区以RO技术为主.我国已建成海水淡化工程141个,总产能1.2×10^(6)m^(3)/d,其中RO和med分别占总产能的63.6%和35.94%.从各种海水淡化技术的应用趋势上看,我国与国际海水淡化产业情况接近.从用户类型看,国际上海水淡化水主要用于市政饮用,而我国海水淡化水主要用于工业领域.

关键词：海水淡化反渗透多效蒸馏多级闪蒸

Classification and management of the tandem ossification of the posterior longitudinal ligament and flaval ligament

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Chinese medical Journal 2009年第2期122卷 219-224页

作者： GUO Jiong-jiong YANG Hui-lin Kenneth m.C. Cheung TANG Tian-si Keith D.K. Luk Department of Orthopaedics the First Affiliated Hospital of Soochow University Suzhou Jiangsu 215006 China Department of Orthopaedics and Traumatology the University of Hong Kong Hong Kong SAR China

Objective To provide appropriate guidelines for treatment of tandem ossification of the posterior longitudinal ligament （OPLL） and flaval ligament （OFL）. data sources Published articles about OPLL and OFL were selected using medline and Embase electronic databases. Study selection An English literature search from January 1980 to December 2006 was conducted. Because many reported cases were incorporated in OFL studies, the key words for search were OFL or OFL and OPLL. The first step revealed 93 studies of which 13 reports of tandem OPLL and OFL （tandem ossification） were selected. Results All studies were case series or case report and advocated that the primary therapy for tandem ossification should be operative. The clinical outcomes of surgery were evaluated in most reports, predominantly using the JOA scores. Gender is the only factor which has prognostic value. A higher proportion of women was found in the failure group A two- stage classification of tandem ossification was developed to relate diagnosis to outcome. Conclusions All patients with suspected ossification of the spinal ligaments should undergo routine mRI screening of the whole spine. The correlation of the classification with surgical treatments needs further studies to validate its usefulness. Chin med J 2009; 122（2）：219-224

关键词： ossification posterior longitudinal ligament flaval ligament ligamentum flavum, tandem

HPV16 L1蛋白在大肠杆菌中的表达、纯化及免疫原性研究

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微生物学杂志 2001年第4期21卷 15-16页

作者：商庆龙谷鸿喜哈尔滨医科大学微生物学教研室黑龙江哈尔滨150086

用IPTG诱导目的工程菌 pQE31 HPV16L1/m 15 (pREP4) ,对表达产物进行SDS PAGE和Westernblot分析 ;用表达的L1蛋白免疫BALB/C小鼠得到抗血清后 ,利用真核源性的VLP粗提物验证小鼠抗血清的特异性。利用ImAC金属亲和层析柱纯化L1蛋白。SDS ... 详细信息

用IPTG诱导目的工程菌 pQE31 HPV16L1/m 15 (pREP4) ,对表达产物进行SDS PAGE和Westernblot分析 ;用表达的L1蛋白免疫BALB/C小鼠得到抗血清后 ,利用真核源性的VLP粗提物验证小鼠抗血清的特异性。利用ImAC金属亲和层析柱纯化L1蛋白。SDS PAGE结果显示表达产物在约 5 7ku处有蛋白条带 ;Westernblot结果证实此条带可与HPV16L1蛋白的单克隆抗体反应 ;纯化后的L1蛋白也同样保留免疫特异性 ;小鼠抗血清可与HPV16L1VLP(病毒样颗粒 )发生特异性反应 ,证实重组表达的L1蛋白具有免疫原性。本实验表明HPV16L1蛋白在工程菌m15 (pREP4)中高效表达。

关键词：人乳头瘤病毒16型 L1蛋白表达免疫原性 HPH16 纯化

Therapy of multiple myeloma

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The Chinese-German Journal of Clinical Oncology 2002年第3期1卷 141-144页

作者： H.Goldschmidt F.W.Crmer Th.möhler A.Krämer m.Görner G.Egerer A.D.Ho Department of Internal medicine V University of Heidelberg Hospitalstr.3 D-68115Germany

Summary multiple myeloma(mm)is characterised by the accumulation of malignant plasma cells in the bone marrow producing a monoclonal *** standard conventional therapy is the combination of melphalan and prednisone resulting in a response rate of 40%-60%and in a.median survival time of approximately 3 *** order to improve the therapeutic efficacy various combination regimens have been *** randomized trials have frailed to show a significant improvement in survival time when combination chemotherapy is used instead of melphalan with or without *** benefit of maintenance therapy with interferon-alpha has been *** toxicity of interferon-alpha,which may reduce the quality of life,should be ***,myeloma-treatment has been ***-dose chemotherapy accompanied by hematopoietic stem-cell support via autologous transplant is recommended up to the age of 65-70 *** results from a French study comparing single versus double autologous transplantation have shown a benefit in terms of event-free survival for the sequential *** as an adoptive immuntherapy to treat minimal residual disease are under *** mortality rale of allogeneic transplantation of hematopoietic stem cells has been reduced in the last 5 *** use of reduced conditioning regimens or the partial depletion of T cells in peripheral blood stem cell transplants in an effort to decrease transplant related mortality are promising ***-omide and its derivates are a new class of agents with independent anti-tumour activity in *** results with this antian-giogenic therapy in phase II trials have been *** therapies,such as the treatment of anaemia with erythropoietin,the management of renal failure and the use of bisphosphonates,improve the life quality of mm patients.

关键词： multiple myeloma treatment transplantation angiogenesis