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He.G2 ce.ls support viral re.lication and ge.e.e.pre.sion of he.atitis C virus ge.otype.4 in vitro

World Journal of Gastroe.te.ology 2006年第30期12卷 4836-4842页

作者： Mostafa K e.-Awady Ashraf A Tabll Yasmine.S e.-Abd Mahmoud M bahgat Husse.n A Shoe. Samar S Yousse. Noha G bade. e. Din e.-Rashdy M Re.wan Maha e.-De.e.lawy Moataza H Omran Wae. T e.-Garf Said A Goue.i De.artme.t of Biomedical Te.hnology National Research Center Tahrir Street PO 12622 Dokki Cairo Egypt De.artme.t of medicinal Che.istry Na- tional Research Center Tahrir Street PO 12622 Dokki Cairo Egypt De.artme.t of Microbiology and Immunology Faculty of Pharmacy Cairo Unive.sity Cairo Egypt Ge.e.ic e.gine.ring and Biote.hnology Re.e.rch Institute New Borg El-Arab City Alexandria Egypt De.artme.t of Pathology School of medicine University of Wisconsin Madison WI United States

AIM： TO e.tablish a ce.l culture.syste. with longte.m re.lication of he.atitis C virus （HCV） ge.ome.and e.pre.sion of viral antige.s in vitro. Me.HODS： He.G2 ce.l line.was te.te. for its susce.tibility to HCV by incubation with a se.um from a patie.t with chronic he.atitis C. Ce.ls and supe.natant we.e.harve.te. at various time.points during the.culture. Culture.supe.natant was te.te. for its ability to infe.t na＇ive.ce.ls. The.pre.e.ce.of minus （antise.se. RNA strand, and the.de.e.tion of core.and e. antige.s in ce.ls we.e.e.amine. by RT-PCR and immunological te.hnique. （flow cytome.ry and We.te.n blot） re.pe.tive.y. Re.ULTS： The.intrace.lular HCV RNA was first de.e.te. on d 3 afte. infe.tion and the. could be.consiste.tly de.e.te. in both ce.ls and supe.natant ove. a pe.iod of at le.st thre. months. The.fre.h ce.ls could be.infe.te. with supe.natant from culture. infe.te. ce.ls. Flow cytome.ric analysis showe. surface.and intrace.lular HCV antige. e.pre.sion using in house.made.polyclonal antibodie. （anti-core. and anti-e.）. We.te.n blot analysis showe. the.e.pre.sion of a cluste. of immunoge.ic pe.tide. at mole.ular we.ghts e.te.de. be.we.n 31 and 45 kDa in an one.month old culture.of infe.te. ce.ls whe.e.s this cluste. was unde.e.table.in uninfe.te. He.G2 ce.ls. CONCLUSION： He.G2 ce.l line.is not only susce.tible.to HCV infe.tion but also supports its re.lication in vitro. e.pre.sion of HCV structural prote.ns can be.de.e.te. in infe.te. He.G2 ce.ls. The.e.ce.ls are.also capable.of she.ding viral particle. into culture.media which in turn be.ome.infe.tious to uninfe.te. ce.ls.

关键词： He.atitis C virus In vitro propagation Ge.omic re.lication Ge.e.e.pre.sion He.G2 ce.ls

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A Comparison of Various Normalization Me.hods for LC/MS Me.abolomics Data

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Advance. in Bioscie.ce.and Biote.hnology 2018年第8期9卷 339-351页

作者： Jacob e. Wulff Matthe. W. Mitche.l Me.abolon Inc. Research Triangle Park NC USA

In me.abolomics data, like.othe. -omics data, normalization is an important part of the.data proce.sing. The.goal of normalization is to re.uce.the.variation from non-biological source. (such as instrume.t batch e.fe.ts), while.maintaining the.biological variation. Many normalization te.hnique. make.adjustme.ts to e.ch sample. One.common me.hod is to adjust e.ch sample.by its Total Ion Curre.t (TIC), i.e. for e.ch fe.ture.in the.sample. divide.its inte.sity value.by the.total for the.sample. Be.ause.many of the.assumptions of the.e.me.hods are.dubious in me.abolomics data se.s, we.compare.the.e.me.hods to two me.hods that make.adjustme.ts se.arate.y for e.ch me.abolite. rathe. than for e.ch sample. The.e.two me.hods are.the.following: 1) for e.ch me.abolite. divide.its value.by the.median le.e. in bridge.sample. (BRDG);2) for e.ch me.abolite.divide.its value.by the.median across the.e.pe.ime.tal sample. (med). The.e.me.hods we.e.asse.se. by comparing the.corre.ation of the.normalize. value. to the.value. from targe.e. assays for a subse. of me.abolite. in a large.human plasma data se.. The.BRDG and med normalization te.hnique. gre.tly outpe.formed the.othe. me.hods, which ofte. pe.formed worse.than pe.forming no normalization at all.

关键词： Me.abolomics Normalization Liquid Chromatography Mass Spe.trome.ry TIC

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The.Synthe.is of a Ne. Macrocyclic Dinucle.ting Ligand,3,6,9,17,20,23-He.aaza-29,30-Dihydroxy-13,27-Dime.hyl-Tricyclo[23,3,1,1^(11,15)]Triaconta-1(28),11,13, 15(30),25,26-He.ae.e

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Chine.e.Che.ical Le.te.s 2000年第7期11卷 573-576页

作者： Guo Qiang SHANGGUAN Arthur e. MARTe.L Zhong Re. ZHANG (De.artme.t of Che.istry, Jining medical Colle.e. Jining 272013De.artme.t of Che.istry, Te.as A&M Unive.sity, Colle.e.Station, Te.as 77842 USA) Jining med Coll Dept Chem Jining 272013 Peoples R China Te.as A&M Univ Dept Chem College Stn TX 77842 USA

A ne. dinucle.ting 24-me.be.e. he.aazadiphe.ol macrocyclic ligand, 3,6,9,17,20, 23-he.aaza-29,30- dihydroxy-13,27-dime.hyl-tricycl [23, 3, 1, 1 11,15] triaconta -1(28), 11,13,15(30),25, 26-he.ae.e. BDBPH, was synthe.ize. by the.NaBH4 re.uction of the.Schiff-base.obtaine. from the.[2+2] conde.sation be.we.n die.hyle.e.riamine.and diformyl -p-cre.ol. The.structure.was characte.ize. by e.e.e.tal analysis, 1HNMR and FAB-MS. The.synthe.ic me.hod was also discusse..

关键词： Macrocyclic ligand synthe.is

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A Me.hanism for Inhale. Ane.the.ic-Induce. Solid Organ Injury: Inflammation

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Ope. Journal of Ane.the.iology 2014年第3期4卷 72-80页

作者： Gary e. Hill Irina Gasanova Ge.ffre. M. Thie.e De.artme.t of Ane.the.iology and Pain Man-age.e.t University of Texas Southwestern Med-ical Center Dallas USA Ve.e.ans Administration Alcohol Re.e.rch Ce.te. Omaha Veterans Administration Medical Center Omaha USA

background: Inhale. ane.the.ics, including halothane. iso- and se.oflurane.induce.proinflammatory cytokine.re.e.se. Halothane.is an inhale. ane.the.ic age.t that is me.abolize. by the.live. into a highly re.ctive.product, trifluoroace.yl chloride. which can re.ct e.doge.ously to form a trifluoroace.yl-adduct (TFA-adduct). The.MAA-adduct is formed by ace.alde.yde.and malondialde.yde.re.cting with e.doge.ousprote.ns and is found in both patie.ts and animals post-consumption of alcohol. The.e.TFA and MAA-adducts have.be.n shown to cause.the.re.e.se.of proinflammatorycytokine. by e.doge.ous inflammatory ce.ls. If both adducts share.a similar me.hanism of ce.l activation, re.e.ving ge.e.al ane.the.ia following alcohol inge.tion could e.ace.bate.the.inflammatory re.ponse.cause. by the.inhale. ge.e.al ane.the.ic halothane.and le.d to solid organ (including live. and brain) injury. Me.hods: Control die. and alcohol-fe. rats we.e.randomize. to re.e.ve.halothane.pre.re.tme.ts by intrape.itone.l inje.tion mixe. in se.ame.oil. Following the.intrape.itone.l inje.tions, the.intact he.rt was re.ove., He.s we.e.isolate. and stimulate. with unmodifie. bovine.se.um albumin (Alb), MAA-modifie. Alb (MAA-Alb), He.yl-MAA, or lipopolysaccharide.(LPS), and supe.natant conce.trations of TNF-α we.e.de.e.mine.. Re.ults: Halothane.pre.tre.te. rat He.s de.onstrate. significantly gre.te. TNF-α conce.tration following MAA-adduct and LPS stimulation than the.non-halothane.pre.tre.te. in both pair and alcohol-fe. rats, but was significantly gre.te. in the.alcohol-fe. groups. Conclusion: The.e.re.ults de.onstrate.that halothane.and MAA-adduct pre.tre.tme.t will incre.se.the.inflammatory re.ponse.(TNF-α re.e.se. in rat He.s following LPS and MAA stimulation in vitro. Also, the.e.re.ults sugge.t that halothane.e.posure.may incre.se.the.risk of alcohol-induce. solid organ injury se.ondary to TNF-induce. inflammation. Othe. inve.tigators have.re.orte. similar proinflammatory cytokine.re.e.se.with othe.

关键词： Ane.the.ic-Induce. Solid Organ Inflammation Halothane MAA

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e.Fe.TS OF SWINe.SPLe.N TRANSFe. FACTOR ON IMMUNe.FUNCTIONS IN PATIe.TS WITH CANGe. OF THe.LARGe.INTe.TINe

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吉林大学学报(医学版) 1991年第3期 238-244页

作者： Wang We.zhong, Jia Huirong, Wang We., Yang De.a Kang Xixiong,Sun Rongwu , Di Liqi, Wang Biao (Ce.tral Lab., De.t.of Surg., De.t.of Inte.n. med., Third Te.ching Hosp.)

Afte. re.e.tion, 29 patie.ts with cance. of the.large.inte.tine.we.e.tre.te. with swine.sple.n transfe. factor (STF) for thre. months. Lymphocyte., subtype. of T lymphocyte., the.le.e.s of IL-2 , IFN, PHA and e.rose.te.formation rate.we.e.compare. be.we.n patie.ts with cance. of the.large.inte.tine.and the.normal one., as we.l as patie.ts pre. and post-tre.te. with *** re.ults indicate. that lymphocyte.,OKT3 + , OKT4 + ,OKT8+,NHK-1 + and the.le.e.s of IL-2 , IFN, PHA positive.transformation rate. e.(e., e.) -rose.te.formation rate. e.c., we.e.marke.ly e.e.ate. following the.use.of STF. It was sugge.te. that STF may le.d the.patie.t suffe.ing from carcinoma to have.the.immune.functions incre.se. or e.e. re.tore., and thus be.be.e.icial during che.othe.apy .That IFN incre.se. doubly afte. the.apy showe. that STF is not only a kind of mitoge., but also an e.ce.le.t inducing age.t of IFN.

关键词： Swine.sple.n transfe. factor (STF) Cance. of the.large.inte.tine.Ce.lular immunity Ce.lular factor

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Pathophysiological conse.ue.ce. of obstructive.jaundice.and pe.iope.ative.manage.e.t

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He.atobiliary & Pancre.tic Dise.se. Inte.national 2018年第1期17卷 17-21页

作者： e.stathios T Pavlidis The.doros e.Pavlidis Aristotle.Unive.sity of The.saloniki Medical School Second Surgical Propedeutic Department Hippocration Hospital

background: Obstructive.jaundice.is a common proble. in daily clinical practice. Unde.standing comple.e.y the.pathophysiological change. in obstructive.jaundice.re.ains a challe.ge.for planning curre.t and future.*** source.: A Pub med was se.rche. for re.e.ant article. publishe. up to August 2016. The.e.fe.t of obstructive.jaundice.on proinflammatory cytokine., coagulation status, he.odynamics and organ functions we.e.***: The.e.fe.ts of obstructive.jaundice.include. biliary tre., the.he.atic ce.l and live. function as we.l as syste.ic complications. The.lack of bile.in the.gut, the.disruption of the.inte.tinal mucosal barrie.,the.incre.se. absorption of e.dotoxin and the.subse.ue.t e.dotoxe.ia cause.proinflammatory cytokine.production(TNF-α, IL-6). Bilirubin induce. syste.ic inflammatory re.ponse.syndrome.which may le.d to multiple.organ dysfunction syndrome. The.principal clinical manife.tations include.he.odynamic instability and acute.re.al failure. cardiovascular suppre.sion, immune.compromise. coagulation disorde.s,nutritional impairme.t, and wound he.ling de.e.t. The.prope. manage.e.t include. full re.lace.e.t of wate. and e.e.trolyte.de.icie.cy, prophylactic antibiotics, lactulose. vitamin K and fre.h froze. plasma,albumin and dopamine. The.pre.pe.ative.biliary drainage.has not be.n indicate. in ove.all, but only in a fe. se.e.te. ***: The.pe.iope.ative.manage.e.t is an e.se.tial me.sure.in improving the.outcome.afte. the.appropriate.surgical ope.ation in jaundice. patie.ts e.pe.ially those.with malignancy.

关键词： Obstructive.jaundice Pe.iope.ative.manage.e.t Pre.pe.ative.biliary drainage Lactulose e.dotoxin Biliary obstruction

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播放详尽的麻醉风险教育视频对患者焦虑情绪及麻醉前访视所用时间的影响：一项随机对照研究

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麻醉与镇痛 2008年第6期4卷 90-97页

作者： Corne.ie.Salzwe.e., Cand. med Corinna Pe.e.se., PhD Irmgard Blanc, MD Uwe.Koch, MD, PhD Alwin e. Goe.z, MD Martin Schuste., MD, MA 王倩(译) 王天龙(校) De.artme.t of Ane.the.iology and Institute.and Policlinic of medical Psychology University Hospital Hamburg-Eppendorf Hamburg Germany 不详

背景麻醉前访视时采用视频辅助方式对患者进行宣传教育是一种新方法，可以增加患者对麻醉过程及风险的了解。但尚不清楚该方法对患者的焦虑情绪以及手术前访视所用时间的影响。方法209例患者，在行大手术前到麻醉门诊进行手术前访视，... 详细信息

背景麻醉前访视时采用视频辅助方式对患者进行宣传教育是一种新方法，可以增加患者对麻醉过程及风险的了解。但尚不清楚该方法对患者的焦虑情绪以及手术前访视所用时间的影响。方法209例患者，在行大手术前到麻醉门诊进行手术前访视，随机分为3组：无视频组（G1）、访视前播放视频组（G2）、访视后播放视频组（G3）。访视前和访视后采用焦虑状态分级和视觉模拟评分考察患者的焦虑程度。访视后采用标准化问卷了解患者对麻醉技术以及相关风险的了解程度和满意度。结果3组之间在焦虑程度以及患者满意度方面没有显著性差异。观看视频的患者麻醉知识了解程度远高于未观看视频的患者。与G1组（17．6±7．2分钟）、G3（18．3±9．6分钟）组相比，G2（23．1±14．0分钟）组患者访视所用时间明显延长。这一差别在拟行有创监测的患者亚组中更为显著。结论本研究结果表明，使用视频对患者进行详尽的麻醉风险教育不能改变患者的焦虑情绪，但可以增加患者对麻醉过程及相关风险的了解。患者在麻醉访视前观看视频，将增加访视所用时间。

关键词：麻醉前访视随机对照研究风险教育焦虑情绪视频手术前访视患者满意度视觉模拟评分

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Se.f-e.pandable.me.al ste.ts for malignant gastric outle. obstruction: A poole. analysis of prospe.tive.lite.ature

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World Journal of Gastroe.te.ology 2015年第43期21卷 12468-12481页

作者： e.o e.van Halse.a e.ik AJ Rauws Paul Focke.s Je.nin e.van Hooft De.artme.t of Gastroe.te.ology and He.atology Academic Medical Center

AIM: To provide.an ove.vie. of the.clinical outcome. of se.f-e.pandable.me.al ste.t(Se.S) place.e.t for malignant gastric outle. obstruction(MGOO).Me.HODS: A syste.atic lite.ature.se.rch was pe.formed in Pub med of the.lite.ature.publishe. be.we.n January 2009 and March 2015. Only prospe.tive.studie. that re.orte. on the.clinical succe.s of ste.t place.e.t for MGOO we.e.include.. The.primary e.dpoint was clinical succe.s,de.ine. according to the.de.inition use. in the.original article. Data we.e.poole. and analyze. using de.criptive.statistics. Subgroup analyse. we.e.pe.formed for partially cove.e. Se.Ss(PCSe.Ss) and uncove.e. Se.Ss(UCSe.Ss) using Fishe.'s e.act ***: A total of 19 studie.,including 1281 patie.ts,we.e.include. in the.final analysis. Gastric(42%) and pancre.tic(37%) cance. we.e.the.main cause. of MGOO. UCSe.Ss we.e.use. in 76% of patie.ts and PCSe.Ss in 24%. The.ove.all poole. te.hnical succe.s rate.was 97.3% and the.clinical succe.s rate.was 85.7%. Ste.t dysfunction occurre. in 19.6% of patie.ts,mainly cause. by re.obstruction(12.6%) and ste.t migration(4.3%),and was comparable.be.we.n PCSe.Ss and UCSe.Ss(21.2% vs 19.1%,re.pe.tive.y,P = 0.412). Re.obstruction was more.common with UCSe.Ss(14.9% vs 5.1%,P e.t migration was more.fre.ue.t afte. PCSe.S place.e.t(10.9% vs 2.2%,P e.ove.all pe.foration rate.was 1.2%. Ble.ding was re.orte. in 4.1% of patie.ts,including major ble.ding in 0.8%. The.median ste.t pate.cy range. from 68 to 307 d in five.studie.. The.median ove.all survival range. from 49 to 183 d in 13 ***: The.clinical outcome. in this large.population showe. that e.te.al ste.t place.e.t was fe.sible.e.fe.tive.and safe. The.e.ore.ste.t place.e.t is a valid tre.tme.t option for the.palliation of MGOO.

关键词： Ste.ts Gastric outle. obstruction Stomach ne.plasm

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Antibody to e. pe.tide.of he.atitis C virus ge.otype.4 inhibits virus binding and e.try to He.G2 ce.ls in vitro

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World Journal of Gastroe.te.ology 2006年第16期12卷 2530-2535页

作者： Mostafa K e.-Awady Ashraf A Tabll Khale. Ate. Samar S Youse. Moataza H Omran Yasmin e.-Abd Noha G bade.-e.din Ahmad M Sale. Samir F Zohny Wae. T e.-Garf De.artme.t of Biomedical Te.hnology National Research CenterCairoEgypt De.artme.t of Bioche.istry Faculty of ScienceAin Shams UniversityCairoEgypt

AIM： To analyze.the.ne.tralizing activity of antibodie. against e. re.ion of he.atitis C virus （HCV）. Spe.ific polydonal antibody was raise. via immunization of Ne. Ze.land rabbits with a synthe.ic pe.tide.that had be.n de.ive. from the.e. re.ion of HCV and was shown to be.highly conse.ve. among HCV publishe. ge.otype.. Me.HODS： Hype.-immune.HCV e. antibodie. we.e.incubate. ove. night at 4 ℃ with se.um sample. positive.for HCV RNA, with viral loads ranging from 615 to 3.2 million IU/mL. Tre.te. se.a we.e.incubate. with He.G2 ce.ls for 90 min. Blocking of viral binding and e.try into ce.ls by anti e. antibody we.e.te.te. by me.ns of RTPCR and flow cytome.ry. Re.ULTS： Dire.t immunostaining using FITC conjugate. e. antibody followe. by Flow cytome.ric analysis showe. re.uce. me.n fluore.ce.ce.inte.sity in sample. pre.incubate. with e. antibody compare. with untre.te. sample.. Furthe.more. 13 out of 18 positive.se.a （72%） showe. comple.e.inhibition of infe.tivity as de.e.te. by RT-PCR. CONCLUSION： In house.produce. e. antibody, blocks binding and e.try of HCV virion infe.tion to targe. ce.ls sugge.ting the.involve.e.t of this e.itope.in virus binding and e.try. Isolation of the.e.antibodie. that block virus attachme.t to human ce.ls are.use.ul as the.ape.tic re.ge.ts.

关键词： Flow cytome.ry He.atitis C virus e. e.ve.ope The.ape.tic antibodie. Dire.t immuno-fluore.ce.ce He.G2 ce.ls

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关于建立我国继续医学教育制度的探讨试点二年来的分析

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继续医学教育 1989年第2期3卷 49-54,99页

作者：屠中元程新洁李晚明陈军浙江医科大学继续医学教育处

本文对我校开展继续医学教育试点二年来的初步结果;开展继续医学教育的迫切性必要性;实施过程中的认识与体会进行了探讨,指出了存在的问题并提出了建议。

关键词：继续医学教育

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