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Cowpea NAC1/NAC2 transcription factors improve growth and tolerance to drought and heat in transgenic cowpea through combined activation of photosynthetic and antioxidant mechanisms

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Journal of Integrative Plant Biology 2023年第1期65卷 25-44页

作者： Richa srivastava Yuriko Kobayashi Hiroyuki Koyama Lingaraj Sahoo Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati Guwahati Assam 781039 India Faculty of Applied Biological Sciences Gifu University 1-1 YanagidoGifu 501-1193Japan

NAC(NAM/ATAF1/2/CUC2)transcription factors are central switches of growth and stress responses in ***,unpredictable interspecies conservation of function and regulatory targets makes the well-studied NAC orthologs inapt for pulse *** knowledge of suitable NAC candidates in hardy pulses like cowpea(Vigna unguiculata(L.)Walp.)is still in infancy,hence warrants immediate biotechnological ***,we showed that overexpression of two native NAC genes(VuNAC1and VuNAC2)promoted germinative,vegetative,and reproductive growth and conferred multiple abiotic stress tolerance in a commercial cowpea *** transgenic lines displayed increased leaf area,thicker stem,nodule-rich denser root system,early flowering,higher pod production(~3.2-fold and~2.1-fold),and greater seed weight(10.3%and 6.0%).In contrast,transient suppression of VuNAC1/2 caused severe growth retardation and flower *** overexpressor lines showed remarkable tolerance to major yielddeclining terminal stresses,such as drought,salinity,heat,and cold,and recovered growth and seed production by boosting photosynthetic activity,water use efficiency,membrane integrity,Na^(+)/K^(+)homeostasis,and antioxidant *** comparative transcriptome study indicated consolidated activation of genes involved in chloroplast development,photosynthetic complexes,cell division and expansion,cell wall biogenesis,nutrient uptake and metabolism,stress response,abscisic acid,and auxin *** their orthologs,VuNAC1/2 direct synergistic transcriptional tuning of stress and developmental signaling to avoid unwanted *** overexpression governs the favorable interplay of photosynthesis and reactive oxygen species regulation to improve stress recovery,nutritional sufficiency,biomass,and *** unconventional balance of strong stress tolerance and agronomic quality is useful for translational crop research and molecular breeding of pulses.

关键词： cowpea enhanced photosynthetic activity improved growth improved yield legume genetic engineering multiple stress tolerance NAC transcription factor VuNAC

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Acclimatization of microalgae Arthrospira platensis for treatment of heavy metals in Yamuna River

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Water Science and Engineering 2020年第3期13卷 214-222页

作者： Nilesh Kumar Shriya Hans Ritu Verma Aradhana srivastava University School of Chemical Technology Guru Gobind Singh Indraprastha UniversityNew Delhi 110078India

Bioaccumulation and biosorption in microalgae are effective approaches for the removal of heavy metals(HMs)from river *** objective of this study was to investigate the potential for use of acclimatized microalgae in the removal of HMs from the Yamuna River water as an acclimatizing *** active culture of Arthrospira platensis(***)was acclimatized to HMs up to a concentration of 100 mg/*** was gradually exposed to increasing concentrations of HMs in five subsequent batches with a step increase of 20 mg/L to acclimatize live cells in the simulated Yamuna River *** presence of high levels of HMs in the Yamuna River water caused growth *** empirical growth inhibition model was developed,and it predicted high threshold concentrations of HMs(210.7e424.5 mg/L),producing a positive specific growth rate of *** also showed high average removal efficiencies of HMs,including 74.0%for Cu,77.0%for Cd,50.5%for Ni,76.0%for Cr,76.5%for Pb,and 63.5%for Co,from HMs-enriched Yamuna River *** findings demonstrated that the maximum specific removal amounts of Cu,Cd,Ni,Cr,Pb,and Co were 54.0,58.0,39.0,62.8,58.9,and 45.3 mg/g,*** maximum yields of the value-added products chlorophyll and phycocyanin were 2.5 mg/g(in a batch of 40 mg/L for Cd)and 1054 mg/g(in a batch of 20 mg/L for Cu),***,acclimatized *** was proven to be a potential microalga not only for sequestration of HMs but also for production of valuable pigments.

关键词： Yamuna River A.platensis Heavy metals Acclimatization Biosorption Pigments

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Synthesis and application of biocompatible nontoxic nanoparticles for reclamation of Ce^3＋ from synthetic wastewater： Toxicity assessment, kinetic, isotherm and thermodynamic study

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Journal of Rare Earths 2018年第9期36卷 994-1006页

作者： Varsha srivastava Sidra Iftekhar ZhaoWang Indu Babu Mika Sillanpaa Laboratory of Green Chemistry School of Engineering Science Lappeenranta University of Technology Sammonkatu 12 FI-50130 Mikkeli Finland Department of Civil and Environmental Engineering Florida International University Miami EL 33174 USA

The aim of present study is to synthesize forsterite nanoparticles（FRST） for the reclamation of cerium ions（Ce3＋） from synthetic *** aim to synthesize FRST nanoparticles is due to its biocompatible and nontoxic *** formation of nanoparticles with average diameter of 58 nm was confirmed by TEM *** images of bare FRST nanoparticles show a heterogeneous surface with porous *** surface area of FRST nanoparticles is calculated to be 33.69 m2/*** significant uptake of Ce3＋ ions can be obtained for all the selected concentrations（25-150 mg/L） within 2 h of adsorbent—adsorbate *** pH study shows that by increasing pH from acidic to alkaline range,higher removal can be *** study demonstrates the endothermic nature of Ce3＋*** value of sticking probability suggests very high sticking probability of Ce3＋ ion for FRST ***3＋ uptake is favored by higher temperature and with the increase in temperature from298 to 328 K,Langmuir adsorption capacity increases from 36.45 to 42.99 m2/*** of FRST nanoparticles was also investigated for other light and heavy rare earth elements in single solute and multisolute systems,FRST nanoparticles show the significant removal of divalent metallic pollutants as *** assessment of chemical toxicity of treated wastewater was carried out with the bioluminescent photobacterium（Vibrio fischeri） and decreased toxicity was observed in treated water *** outcome of present study suggests that the FRST nanoparticles can be efficiently utilized for the removal of Ce3＋ ions and a wide range of other pollutant species as well.

关键词： Forsterite Rare earth elements Isotherm model Cerium Metallic pollutants

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Application of IITM-RANS3D to free-fall water entry of prismatic and non-prismatic finite wedges

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水动力学研究与进展B辑:英文版 2023年第3期35卷 417-430页

作者： Shaswat Saincher Kshitij srivastava R.Vijayakumar V.Sriram Center for Maritime Experiments to Maritime Experience(ME2ME) Indian Institute of Technology MadrasChennaiIndia Department of Ocean Engineering Indian Institute of Technology MadrasChennaiIndia

High-speed watercraft and ships undergo coupled motions which make the front portion of the hull exit and violently re-enter *** induces short-term slamming loads that may compromise the structural integrity of the *** slamming of the bow can be modeled as straight wedges of different deadrise angles(DRAs)falling into water from different *** advent of computational fluid dynamics has allowed the problem of wedge-slamming to be simulated using the full Navier-Stokes equations thus complementing the pioneering studies based on ***,most researchers are opting to use commercial software to simulate the wedge-impact problem as it allows access to overset meshing algorithms which are robust in modeling the wedge as a moving *** boundary methods(EBMs)offer some advantages over overset meshing in that the mesh only needs to be generated once and Cartesian mesh-based solvers can be ***,the application of EBMs to wedge-impact has been limited in the literature and merits further *** this context,we investigate the applicability of the fast-fictitious-domain(FFD)based embedded boundary treatment to simulate the violent water-entry of *** extend our in-house Navier-Stokes model IITM-RANS3D to handle floating bodies through integration of a rigid-body dynamics solver and an algorithm to embed three-dimensional stereolithography(STL)geometries as solids over a Cartesian *** proposed algorithm is extensively benchmarked against variable DRA wedge-slamming experiments reported in the literature as well as constant DRA wedge-slamming experiments performed *** good agreement is reported in terms of the time-history of hydrodynamic impact pressures measured at various locations on the hull as well as the wedge motion responses thus demonstrating the suitability of FFD for simulating the coupled hydrodynamics of slamming for simplified hull geometries.

关键词： Wedge water entry slamming fast-fictitious-domain IITM-RANS3D experiments

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A multirate DSP structure for the identification of protein-coding regions

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International Journal of Biomathematics 2017年第8期10卷 125-139页

作者： Malaya Kumar Hota Vinay Kumar srivastava School of Electronics EngineeringVIT University Vellore 632014 Tamilnadu India Department of Electronics and Communication Engineering Motilal Nehru National Institute of Technology Allahabad 211004 Uttar Pradesh India

The identification of protein-coding regions in DNA sequence using digital signal process- ing methods is one of the central issues in bioinformatics. In this paper, a multirate struc- ture is proposed for the identification of protein-coding regions whose input sampling rate is same as output sampling rate. The multirate structure consists of cascade com- bination of decimation filter, kernel filter and interpolation filter. The decimation filter is a complex filter, the kernel filter is an FIR lowpass filter and the interpolation filter is a moving average filter. Polyphase decomposition is applied on both decimation filter and interpolation filter for computationally efficient implementation. The potential of the proposed method is evaluated in comparison with existing methods using standard datasets. The results show that the proposed method improves the identification accu- racy of protein-coding regions to a great extent compared to its counterparts.

关键词： Bioinformatics protein-coding regions multirate structure polyphase decom-position period-3 property.

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Highlights from the prostate cancer genome report

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Asian Journal of Andrology 2011年第5期13卷 659-660页

作者： Shyh-Han Tan Gyorgy Petrovics Shiv srivastava Center for Prostate Disease Research Department of Surgery Uniformed Services University of the Health Sciences Rockville MD 20852 USA

Prostate cancer （CAP） is the second most frequently diagnosed cancer of men worldwide （899 000 new cases, 13.6% of the total）, with nearly 75% of the registered cases occurring in developed countries （644 000 cases）.1 Blood prostate-specific antigen test has revolutionized the early detection of CaP and organ-confined CaP is effectively managed by state-of-the-art treatments including radical prostatectomy or radiation therapy.2 In the past decade, tremendous progress has also been made in our understanding of the biology and common genomic alterations in CaP.3＇4 New molecular marker assays have promise in improving CaP diagnosis.5-8 Despite these advances, major challenges remain with our ability to distinguish indolent cancers from the more aggressive cancers detected early due to widely used prostate-specific antigen test. Furthermore, development of molecular stratification of CaP for targeted and more effective therapies is critically needed.

关键词： Cytokines Tuberculosis

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MULTISTAGE FILTERS FOR IDENTIFICATION OF EUKARYOTIC PROTEIN CODING REGIONS

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International Journal of Biomathematics 2012年第2期5卷 43-60页

作者： MALAYA KUMAR HOTA VINAY KUMAR srivastava Department of Electronics and Communication Engineering Motilal Nehru National Institute of Technology Allahabad-211004 Uttar PradeshIndia

A class of multistage filters, namely, real narrowband bandpass filter （RNBPF） has been previously used for identification of protein coding regions. This filter passes the frequency component at 2π/3 along with its conjugate. This conjugate frequency compo- nent may degrade the identification accuracy. To improve the identification accuracy, two types of multistage filters are proposed in this paper. A complex narrowband bandpass filter （CNBPF） is proposed for suppressing the conjugate frequency component which, in turn, reduces the background noise present in the deoxyribonucleic acid （DNA） spec- trum and improves identification accuracy. By cascading RNBPF with moving average filter （RNBPFMA）, another type of multistage filter is proposed. As moving average filter smooth out the rapid variations in the DNA spectrum, RNBPFMA improves the identification accuracy. The computational complexity of RNBPFMA is less than that of CNBPF. The RNBPF and proposed multistage filters are compared with previously reported short-time discrete Fourier transform （ST-DFT） method in terms of compu- tational complexity. It is found that multistage filters reduce the computational load to a greater extent compared to ST-DFT method. The identification accuracy of the proposed CNBPF and RNBPFMA methods is compared with existing anti-notch filter and RNBPF methods. The results show that proposed methods outperform existing methods in terms of identification accuracy for benchmark data sets.

关键词： Bioinformatics multistage filter protein coding region period-3 property narrowband bandpass filter genomic signal processing.

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A near infrared optical reflector using one-dimensional photonic crystal structure containing chalcogenide glasses

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Optoelectronics Letters 2010年第6期6卷 406-411页

作者： Sanjeev K.srivastava S K Awasthi S K srivastava S.P.Ojha Department of Physics Amity Institute of Applied SciencesAmity University Department of Physics Jaypee Institute of Information TechnologyDeemed University Department of Physics Mahila Maha VidyalayBanaras Hindu University Department of Applied Physics Institute of TechnologyBanaras Hindu University

The reflectivity of one-dimensional chalcogenide photonic crystal(CGPC) structure with the first order reflection band in near infrared(NIR) region is theoretically ***-Se and Ge-S chalcogenide glasses are used as high and low refractive index layers respectively,because these materials have zero absorption in NIR *** transfer matrix method(TMM) is employed to calculate the reflective spectra of the proposed *** theoretical results of reflective spectra of bulk chalcogenide materials with the composition of Sb40Se60 and Ge30S70 for 4,8,12 and 15 layers and thicknesses of 117 nm and 183 nm respectively,at normal incidence,are close agreement with the experimental ***,by increasing the number of layers of Sb40Se60 and Ge30S70,the reflection bands can be enhanced in the wider range of the NIR region for the polarization at different angles and thus the broadband omnidirectional reflector can be designed.

关键词：一维光子晶体晶体结构硫系玻璃近红外光反射镜红外区域反射光谱计算结果

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Controlling Diffusion by Varying Width of Layers in Nano Channel

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Nano-Micro Letters 2012年第3期4卷 154-157页

作者： Ishu Goyal Sunita srivastava K.Tankeshwar Department of Physics Panjab University Computer Centre Panjab University

Diffusive dynamics of fluid forming layers of high and low density regions in a nanochannel has been *** coefficient in direction parallel and perpendicular to the confining wall has been found to show behaviour which is not observed in micro channel or bulk *** behaviour of diffusion is found to be controlled by the width of layers formed in nanochannel due to wall and particle *** is an important result as width of layers and hence flow of fluid inside nano pores/tube can be controlled by an external source.

关键词： Nanochannel Layer Diffusion

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Compositionally Graded Microstructure for Ag-Fe Nanoparticles

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Nano-Micro Letters 2012年第3期4卷 172-175页

作者： Chandan srivastava K.V.L.Sushma Department of Materials Engineering Indian Institute of Science

Ag-Fe nanoparticles with a highly Ag rich average composition were synthesized by the sonochemical ***-iron system exhibits a wide miscibility gap in the bulk ***,a graded compositional profile along the nanoparticle radius was *** at and near the surface of the nanoparticle contained both Ag and Fe *** composition got relatively deficient Fe towards the center of the particle with particle core made up of pure *** of Ag and Fe is confirmed by the absence of diffraction signal corresponding to pure Fe phase and presence of a paramagnetic phase in nanoparticles containing a diamagnetic(Ag)and ferromagnetic(Fe)elements.

关键词： Nanoparticles Electron Microscopy Ag-Fe Miscibility gap Solid solution Microstructure

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