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M1-polar.zed macr.pha.e.der.ved cellular.nanovesicle-coated lipid nanopar.icles for.enhanced cancer.tr.atment thr.ugh hybr.dization of gene ther.py and cancer.immunother.py

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Acta Phar.aceutica Sinica B 2024年第7期14卷 3169-3183页

作者： ha.e.n Shin Jun-Hyeok han Seungyong Shin Ga-Hyun Bae Bor.m Son Tae-Hyung Kim Hee Ho Par. Chun Gwon Par. Woor.m Par. Depar.ment of Integr.tive Biotechnology Sungkyunkwan University(SKKU)SuwonGyeonggi 16419Republic of Korea Depar.ent of Inteligent Pr.cision Healthcar. Conver.ence SKKUSuwonGyeonggi 16419Republic of Korea Depar.ment of MetaBioHealth SKKU Institute for ConvergenceSKKUSuwonGyeonggi 16419Republic of Korea Depar.ment of Bioengineer.ng Hanyang UniversitySeoul 04763Republic of Korea Depar.ment of Integr.tive engineer.ng Chung-Ang UniversitySeoul 06974Republic of Korea Depar.ment of Biomedical engineer.ng SKKUSuwonGyeonggi 16419Republic of Korea Kor.a Institute of Science and Technology(KIST) Seoul 02792Republic of Korea

Optimum genetic deliver. for.modulating tar.et genes to diseased tissue is a major.obstacle for.pr.fitable gene *** nanopar.icles(LNPs),consider.d a pr.spective vehicle for.nucleic acid deliver.,ha.e.demonstr.ted efficacy in human use dur.ng the COVID-19 *** study intr.duces a novel biomater.al-based platfor.,M1-polar.zed macr.pha.e.der.ved cellular.nanovesicle-coated LNPs(M1-C-LNPs),specifically engineer.d for.a combined gene-immunother.py appr.ach against solid *** dual-function system of M1-C-LNPs encapsulates Bcl2-tar.eting sir.A within LNPs and immune-modulating cytokines within M1 macr.pha.e.der.ved cellular.nanovesicles(M1-NVs),effectively facilitating apoptosis in cancer.cells without impacting T and NK cells,which activate the intr.tumor.l immune r.sponse to pr.mote gr.nule-mediating killing for.solid tumor.*** r.tention within tumor.was obser.ed upon intr.tumor.l administr.tion of M1-C-LNPs,owing to the pr.sence of adhesion molecules on M1-NVs,ther.by contr.buting to super.or.tumor.gr.wth *** findings r.pr.sent a pr.mising str.tegy for.the development of tar.eted and effective nanopar.icle-based cancer.genetic-immunother.py,with significant implications for.advancing biomater.al use in cancer.ther.peutics.

关键词： Genetic-immunother.py M1 macr.pha.e.der.ved cellular.nanovesicles Lipid nanopar.icles(LNPs) Gene ther.py sir.A Cancer.immunother.py Solid tumor Tumor.micr.envir.nment(TMe)

Gener.tion of double knockout cattle via Cr.SPr.Cas9 r.bonucleopr.tein(r.P)electr.por.tion

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Jour.al of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi Jae-Jung ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Labor.tor. of Ther.ogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LAr.Bio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock r.sear.h Institute YeongjuRepublic of Korea Compar.tive Medicine Disease r.sear.h Center Seoul National UniversitySeoulRepublic of Korea

Backgr.und Genome editing has been consider.d as power.ul tool in agr.cultur.l ***,genome editing pr.gr.ss in cattle has not been fast as in other.mammal species,for.some disadvantages including long gestational per.ods,single pr.gnancy,and high r.ising ***,technically demanding methods such as micr.injection and somatic cell nuclear.tr.nsfer.SCNT)ar. needed for.gene editing in *** this point of view,electr.por.tion in embr.os has been r.sen as an *** Fir.t,editing efficiency of our.electr.por.tion methods wer. tested for.*** of mutation on embr.o was confir.ed by T7e1 *** fir.t combination,mutation r.tes for.MSTN and Pr.P wer. 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation r.tes wer. 83.9%±23.6%for.MSTN,84.5%±18.0%for.***,the double-KO embr.os wer. tr.nsfer.ed to sur.ogates and mutation r.te was identified in r.sultant calves by tar.eted deep *** r.cipients wer. tr.nsfer.ed for.MSTN/Pr.P,4 calves wer. deliver.d,and one calf under.ent an induction for.double *** sur.ogates wer. given double-KO embr.os for.MSTN/BLG,and four.of the six calves that wer. bor. had mutations in both *** These data demonstr.ted that pr.duction of genome edited cattle via electr.por.tion of r.P could be effectively ***,MSTN and Pr.P fr.m beef cattle and MSTN and BLG fr.m dair. cattle ha.e.been bor. and they will be valuable r.sour.es for.futur. pr.cision br.eding.

关键词： Beta-lactoglobulin Cattle Cr.SPr.Cas9 electr.por.tion Knockout MSTN Pr.P

Antibacter.al ha-coatings on bior.sor.able Mg alloy

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Jour.al of Magnesium and Alloys 2024年第5期12卷 1965-1985页

作者： K.V.Nadar.ia D.V.Mashtalyar.M.A.Piatkova A.I.Pleshkova I.M.Imshinetskiy M.S.Ger.simenko e.A.Belov V.V.Kumeiko D.N.Kozyr.v K.A.Fomenko V.V.Mostovaya B.r.Tor.anov A.r.Biktimir.v I.S.Osmushko S.L.Sinebr.ukhov S.V.Gnedenkov Institute of Chemistr. FeB r.S 159 Pr.100-letiya VladivostokaVladivostok 690022Russia Far.easter. Feder.l Univer.ity 10 Ajax BayRussky IslandVladivostok 690922Russia A.V.Zhir.unsky National Scientific Center.of Mar.ne Biology FeB r.S 17 St.PalchevskogoVladivostok 690041Russia r.gional Clinical Der.atovener.logical Dispensar. 18 V St.GamarnikaVladivostok 690033Russia NMIC“Tr.atment and r.habilitation Center. 3 Ivankovskoe highwayMoscow 125367Russia

In this study,a calcium-phospha.e.coating was for.ed on a Mg-Mn-Ce alloy by the plasma electr.lytic oxidation(PeO).The antibiotic vancomycin,widely used in the tr.atment of infections caused by Staphylococcus aur.us(***),was impr.gnated into the *** with vancomycin showed high bacter.cidal activity against *** mechanical and electr.chemical pr.per.ies of the for.ed coatings wer. studied,as well as in vitr. cytotoxicity tests and in vivo tests on matur. male r.ts wer. *** to SeM,eDS,Xr. and XPS data,coatings had a developed mor.hology and contained hydr.xyapatite,which indicates high *** analysis of r.ughness of coatings without and with vancomycin did not r.veal any differ.nces,confir.ing the high r.ughness of the *** electr.chemical tests,an incr.ase in cor.osion r.sistance by mor. than two times after.the application of PeO coatings was *** to the r.sults of an in vivo study,after.28 days of the implantation of samples with calcium phospha.e.PeO coating and vancomycin,no signs of inflammation wer. obser.ed,while an inflammator. r.action developed in the ar.a of implantation of bar. alloy,followed by *** r.lease tests fr.m the coatings show a shar. decr.ase in the concentr.tion of the r.leased antibiotic on day 7 and then a gr.dual decr.ase until day *** the exper.ment,no significant deviations in the condition and behavior.of the animals wer. obser.ed;clinical tests did not r.veal a systemic toxic r.action.

关键词： Bioactive coatings Biocompatibility Mg alloy Plasma electr.lytic oxidation Hydr.xyapatite Vancomycin

An oxygenating colloidal bioink for.the engineer.ng of biomimetic tissue constr.cts

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Bio-Design and Manufactur.ng 2024年第3期7卷 240-261页

作者： Seol-ha Jeong Jar.o Hiemstr. Patr.ck V.Blokzijl r.beca Damian-Fer.ar. Danilo Mar.ins dos Santos Jéssica H.L.da Fonseca Min-Ho Kang Jihyun Kim Dilar. Yilmaz-Aykut Mei L.L.Cham-Pér.z Jer.en Leijten Su r.on Shin Division of engineer.ng in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA Leijten Lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnology National Labor.tor. for.Agr.cultur.(LNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Depar.ment of Biomedical-Chemical engineer.ng The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Depar.ment of Biotechnology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

ensur.ng a sufficient oxygen supply is pivotal for.the success of biopr.nting applications since it foster. tissue integr.tion and natur.l *** in oxygen concentr.tion among diver.e tissues necessitates the pr.cise r.cr.ation of tissue-specific oxygen levels in impr.nted constr.cts to suppor. the sur.ival of tar.eted *** oxygen-r.leasing biomater.als,such as oxygen-gener.ting micr.par.icles(OMPs),ha.e.shown pr.mise for.enhancing the oxygen supply of micr.envir.nments in injur.d tissues,whether.this appr.ach is scalable for.lar.e tissues and whether.tissue-specific bioinks with var.ing OMP concentr.tions r.main pr.ntable r.main *** study addr.sses this cr.tical gap by intr.ducing an innovative class of engineer.d oxygenated bioinks that combine colloidal-based micr.gels with *** r.por. that incor.or.ting nanosized calcium per.xide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydr.phobic polymer.c micr.par.icles enables pr.cise modulation of oxygen r.lease while contr.lling hydr.gen per.xide ***,the fabr.cation of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-pha.e.*** study thor.ughly evaluates the fundamental char.cter.stics of the r.sulting bioink,including its r.eological behavior.,pr.ntability,sha.e.fidelity,mechanical pr.per.ies,and oxygen r.lease ***,this study demonstr.tes the macr.scopic scalability and cytocompatibility of pr.nted constr.cts pr.duced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extr.sion-based biopr.nting,this study under.cor.s how it can be used to fabr.cate biomimetic tissues,indicating its potential for.new *** findings pr.sented her. advance the biopr.nting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** wor. ther.by offer. a pr.mising avenue for.the development of functional tissues with e

关键词： 3D biopr.nting Bioink Colloidal gels extr.sion pr.nting Oxygen-gener.ting micr.par.icle

Adding Value to Cr.p Pr.duction Systems by Integr.ting For.ge Cover.Cr.p Gr.zing

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Amer.can Jour.al of Plant Sciences 2024年第3期15卷 180-192页

作者： r.ber. B. Mitchell Dar.n D. r.dfear. Kenneth P. Vogel Ter.y J. Klopfenstein Galen er.ckson P. Stephen Baenziger.Br.ce e. Ander.on Mar. e. Dr.wnoski Jay Par.ons Steven D. Master.on Mar.y r. Schmer.Vir.inia L. Jin USDA-Ar. Wheat Sorghum and Forage Research Unit University of Nebraska-Lincoln Lincoln NE USA Depar.ment of Agr.nomy and Hor.icultur. University of Nebraska-Lincoln Lincoln NE USA Depar.ment of Animal Science University of Nebraska-Lincoln Lincoln NE USA Depar.ment of Agr.cultur.l economics University of Nebraska-Lincoln Lincoln NE USA USDA-Ar. Agroecosystem Management Research Unit University of Nebraska-Lincoln Lincoln NE USA

In addition to their.value as cer.al gr.ins, wheat (Tr.ticum aestivum L.) and tr.ticale (× Tr.ticosecale Wittmack) ar. impor.ant cool-season annual for.ges and cover.cr.ps. Year.ing steer.(Bos taur.s) per.or.ance was compar.d in the spr.ng following autumn establishment as for.age cover.cr.ps after.soybean [Glycine max (L.) Mer..] gr.in har.est. r.plicated pastur.s (0.4 ha) wer. no-till seeded in thr.e consecutive year. into soybean stubble in autumn, fer.ilized, and gr.zed the following spr.ng near.Ithaca, Ne, USA. each pastur. (n = 3) was continuously stocked in spr.ng with four.year.ing steer. (380 ± 38 kg) for.17, 32, and 28 d in 2005, 2006, and 2007, r.spectively. In 2005, aver.ge daily gain (ADG) for.steer. gr.zing tr.ticale exceeded the ADG for.wheat by 0.31 kghd-1d-1. In 2006, wheat ADG exceeded that for.tr.ticale by 0.12 kghd-1d-1. In 2007, steer. gr.zing wheat lost weight, while steer. gr.zing tr.ticale gained 0.20 kghd-1d-1. Based on the 3-year.aver.ge animal gains valued at $1.32 kg-1, mean net r.tur. ($ ha-1 yr.sup>-1) was $62.15 for.tr.ticale and $22.55 for.wheat. Since these gr.zed cover.cr.ps pr.vide ecosystem ser.ices in addition to for.ge, gr.zing could be viewed as a mechanism for.r.cover.ng costs and adds additional value to the system. Based on this 3-year.gr.zing tr.al, tr.ticale was super.or.to wheat and likely will pr.vide the most stable beef year.ing per.or.ance acr.ss year. with var.able weather.for.the wester. Cor.belt USA.

关键词： Cover.cr.ps Soybean Tr.ticale Wheat

Near.Sur.ace Soil Chemical Pr.per.ies as Affected by Cover.Cr.ps Over.Time in the Lower.Mississippi r.ver.Valley

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Agr.cultur.l Sciences 2024年第9期15卷 1035-1056页

作者： Cooper.Fanning Kr.stofor.r. Br.e Michael B. Daniels Tr.nton L. r.ber.s Samuel Fer.andes Lisa S. Wood Depar.ment of Cr.p Soil and Environmental Sciences University of Arkansas Fayetteville USA

Typical r.w-cr.p agr.cultur.l pr.ctices can potentially be har.ful to soil health and futur. sustainability. The use of cover.cr.ps (CC) as a mechanism to impr.ve soil health on a wide scale r.mains under.tilized. Soil health r.mains a major.concer. for.the sustainability of agr.cultur.l pr.ductivity, ther.for., r.sear.h into CC implementation as a mean to pr.ser.e or.impr.ve soil health is war.anted. The objective of this study was to evaluate the effects of CC on the soils in the easter. Ar.ansas por.ion of the Lower.Mississippi r.ver.Valley (LMr.) over.time for.var.ous chemical soil par.meter., including pH, soil or.anic matter.(SOM), soil elemental contents (i.e., P, K, Ca, Mg, S, Na, Fe, Mn, Zn, Cu, and B), soil r.spir.tion, and a gener.lized soil health scor. index. Soil pH decr.ased over.time under.both CC and no-cover.cr.p (NCC) tr.atments, by −0.3 and −0.2, r.spectively. Soil OM decr.ased over.time under.NCC by −0.1%, but did not differ.between CC tr.atments. Soil N availability decr.ased over.time under.NCC (−22.6 kg·ha−1), but did not change over.time under.CC. Soil r.spir.tion decr.ased over.time under.both CC and NCC, by −76.1 mg·L−1 and −77.3 mg·L−1, r.spectively, though ther. was no effect of CC tr.atment. The ha.e. soil health scor. index decr.ased under.CC (−7.0) and NCC (−6.8) without an effect fr.m CC tr.atment. r.sults of the study place emphasis on the tempor.l natur. of soil health as influenced by cover.cr.ps and their.potential to impr.ve soil health.

关键词： Ar.ansas Cover.Cr.ps Soil Pr.per.ies Soil Or.anic Matter Soil Health Scor.

Abovegr.und car.on sequestr.tion of Cunninghamia lanceolata for.sts:Magnitude and dr.ver.

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For.st ecosystems 2024年第1期11卷 32-41页

作者： Chen Wang Shuguang Liu Yu Zhu Andr.w r.Smith Ying Ning Deming Deng National engineer.ng Labor.tor. for.Applied Technology of For.str.&ecology in South China Central South University of Forestry and TechnologyChangsha 410004China Technology Innovation Center.for.ecological Conser.ation and r.stor.tion in Dongting Lake Basin Ministry of Natural ResourcesChangsha 410007China College of Life Science and Technology Central South University of Forestry and TechnologyChangsha 410004China School of ecology and envir.nment Hainan UniversityHaikou 570228China School of Natur.l Sciences Bangor UniversityGwyneddLL572UWUK College of For.str. Central South University of Forestry and TechnologyChangsha 410004China Hunan Pr.specting Designing&r.sear.h Gener.l Institute for.Agr.cultur. Forestry&IndustryChangsha 410007China

Under.tanding the spatial var.ation,tempor.l changes,and their.under.ying dr.ving for.es of car.on sequestr.tion in var.ous for.sts is of gr.at impor.ance for.under.tanding the car.on cycle and car.on management *** car.on density and sequestr.tion in var.ous Cunninghamia lanceolata for.sts,extensively cultivated for.timber.pr.duction in subtr.pical China,var. with biodiver.ity,for.st str.ctur.,envir.nment,and cultur.l factor. r.main poor.y explor.d,pr.senting a cr.tical knowledge gap for.r.alizing car.on sequestr.tion supply potential thr.ugh *** on a lar.e-scale database of 449 per.anent for.st inventor. plots,we quantified the spatial-tempor.l heter.geneity of abovegr.und car.on densities and car.on accumulation r.tes in Cunninghamia lanceolate for.sts in Hunan Pr.vince,China,and attr.buted the contr.butions of stand str.ctur.,envir.nmental,and management factor. to the heter.geneity using quantile age-sequence analysis,par.ial least squar.s path modeling(PLS-PM),and hot-spot *** r.sults showed lower.values of car.on density and sequestr.tion on aver.ge,in compar.son with other.for.sts in the same climate zone(i.e.,subtr.pics),with pr.nounced spatial and tempor.l ***,quantile r.gr.ssion analysis using car.on accumulation r.tes along an age sequence showed lar.e differ.nces in car.on sequestr.tion r.tes among under.er.or.ed and outper.or.ed for.sts(0.50 and 1.80 Mg·ha^(-1)·yr.(-1)).PLS-PM demonstr.ted that maximum DBH and stand density wer. the main cr.cial dr.ver. of abovegr.und car.on density fr.m young to matur. ***,species diver.ity and geotopogr.phic factor. wer. the significant factor. causing the lar.e discr.pancy in abovegr.und car.on density change between low-and high-car.on-bear.ng *** analysis r.vealed the impor.ance of cultur. attr.butes in shaping the geospatial patter.s of car.on *** wor. highlighted that r.taining lar.esized DBH tr.es and incr.asing sha.e.t

关键词： Car.on density Car.on accumulation r.te For.st age Spatial var.ation Cultur.l influence

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沙地云杉种子内参基因的筛选与验证

分子植物育种 2024年第8期22卷 2600-2610页

作者：李佳琪白玉娥吉仁花哈布尔娜苏勒玛内蒙古农业大学林学院呼和浩特010000 内蒙古蒙荣园林绿化工程有限公司呼和浩特010000

为筛选出沙地云杉(Picea mongolica)种子成熟过程中均可稳定表达的内参基因,本研究以沙地云杉种子不同发育时期的样本为研究对象,采用r.-qPCr.术,检测沙地云杉种子转录组数据库中GAPDH、eF1α、CYC、Actin等8个候选内参基因在种子发育... 详细信息

为筛选出沙地云杉(Picea mongolica)种子成熟过程中均可稳定表达的内参基因,本研究以沙地云杉种子不同发育时期的样本为研究对象,采用r.-qPCr.术,检测沙地云杉种子转录组数据库中GAPDH、eF1α、CYC、Actin等8个候选内参基因在种子发育过程中呈现的综合表达水平,并通过内参基因分析软件(geNor.,Nor.Finder.BestKeeper.对其稳定性统一评估。结果表明,候选内参基因中稳定性最好的是CYC、eF1α和GAPDH。分别以CYC、eF1α和GAPDH作为内参基因对沙地云杉胚胎发育关键调控基因LeA1(Late embr.ogenesis abundant pr.tein)和WOX9 (WUSCHeL r.lated homebox)进行了定量分析,其表达趋势基本一致。本研究结果将对沙地云杉及近缘物种的分子育种研究提供参考。

关键词：沙地云杉(Picea mongolica) 内参基因 r.-qPCr 基因表达

基于最小平均距离免疫算法的模糊红外图像分割（英文）

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光谱学与光谱分析 2018年第11期38卷 3645-3652页

作者：于晓周子杰 Kamil r.ha 天津理工大学电气电子工程学院天津市复杂系统控制理论与应用重点实验室天津300384 Depar.ment of Telecommunications Brno University of TechnologyBRNO 61200Czech Republic

由于犯罪分子利用各种方法来避开传统的刑侦图像技术,因而红外图像逐渐成为获取犯罪现场痕迹的有效手段。然而,从犯罪现场拍摄的红外图像其目标痕迹大多是弱化的,所以在这类红外图像中分割目标是一项具有挑战性的任务。已有基于生物免... 详细信息

由于犯罪分子利用各种方法来避开传统的刑侦图像技术,因而红外图像逐渐成为获取犯罪现场痕迹的有效手段。然而,从犯罪现场拍摄的红外图像其目标痕迹大多是弱化的,所以在这类红外图像中分割目标是一项具有挑战性的任务。已有基于生物免疫的各类算法尚未明确描述免疫分割作用领域,以及免疫网络算法模型中的免疫识别距离。为实现对目标痕迹弱化红外图像的有效分割,提出了一种新的具有免疫作用领域和最小平均免疫识别距离的人工免疫构架,设计了一种具备最小平均距离免疫域的免疫分割算法。该方法根据红外图像的特点,采用多步分类算法、免疫变异和自适应免疫最小均距识别方法,根据目标区域和背景区域的总体统计特性实现最佳分类。实验结果表明,提出的基于最小平均距离的免疫算法能够有效地分割目标弱化的红外图像。与经典的边缘模板和区域模板方法相比,该算法具有更好的分割效果,尤其是针对目标弱化红外图像的分割,该算法能够较好地给出五个手指的边界轮廓。

关键词：模糊红外图像图像分割免疫域