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Nrf2-mediated antioxidant and detoxifying enzyme induction by a combination of curcumin and sulforaphane

Journal of Chinese Pharmaceutical Sciences 2016年第8期25卷 559-569页

作者： Francisco Fuentes Yury gomez Ximena Paredes-Gonzalez Avantika Barve Sujit Nair Siwang Yu Constance Lay Lay Saw Ah-Ng Tony Kong Facultad de Agronomia e Ingenieria Forestal Pontificia Universidad Cato1ica de Chile Casilla 306-22 Santiago Chile Department of Pharmaceutics Ernest Mario School of Pharmacy Rutgers The State University of New Jersey New Jersey 08854 USA Novartis Institutes for Biomedical Research East Hanover NJ-07470 USA Amrita cancer Discovery Biology Laboratory Amrita Vishwa Vidyapeetham University Amritapuri Clappana P.O. Kollam Kerala-690525 India School of Pharmaceutical Sciences Peking University Health Science Center Beo'ing 100191 China

The dietary phytochemicals curcumin （CUR） and sulforaphane （SFN） have shown remarkable cancer chemopreventive effects in many model systems. This study was designed to investigate the induction of Nrf2-mediated antioxidant enzymes by combining doses of CUR and SFN and the effect of their combination on the Nrf2-ARE （antioxidant response element） response in HepG2-C8 cells. We hypothesized that the combination of the polyphenol CUR and the isothiocyanate SFN could enhance the induction of AREs and Nrf2-target enzymes. HepG2-C8 cells were treated with a combination of low doses of CUR, SFN or both. The induction of Nrf2-mediated antioxidant and phase II detoxifying enzymes-heme oxygenase-1 （HO-I） and UDP-glucuronosyltransferase-1A （UGT1A）-was measured by real-time RT-PCR and western blotting. ARE-luciferase activity was also quantified. Low doses of CUR （10 ~tM） and SFN （12.5 ~tM） significantly induced the expression of HO-1 and UGT 1 A1 proteins. Through the use of chemical inhibitors of mRNA and protein synthesis, the combination of CUR and SFN was shown to affect the transcriptional regulation of both HO-1 and UGT1A1. Additionally, the combination of CUR and SFN synergistically induced the expression of Nrf2- and ARE-luciferase activity in HepG2-C8 cells. Thus, CUR and SFN at low concentrations augment therapeutic effects in HepG2-C8 cells. The enhanced ARE-luciferase activity of combined CUR and SFN treatment could partly explain the significant induction of the Nrf2-target enzymes HO-1 and UGT1A1. Taken together, our results suggest that combining low doses of CUR and SNF could be a promising strategy for cancer chemoprevention in humans.

关键词： Antioxidant response element, Curcumin, HepG2-C8 cell-s, HO-1, Nrf2, Sulforaphane, UGT 1A1

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Synthesis and characterization of nano-hydroxyapatite added with magnesium obtained by wet chemical precipitation

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Progress in Natural Science:Materials International 2021年第4期31卷 575-582页

作者： Brandon A.Correa-Pi?a Omar M.gomez-Vazquez Sandra M.Londo?o-Restrepo Luis F.Zubieta-Otero Beatriz M.Millan-Malo Mario E.Rodriguez-García Posgrado en Ciencia e Ingeniería de Materiales Centro de Física Aplicada y Tecnología Avanzada Universidad Nacional Autónoma de México Campus Juriquilla Departamento de Nanotecnología Centro de Física Aplicada y Tecnología Avanzada Universidad Nacional Autónoma de México Campus Juriquilla

Biogenic hydroxyapatites from mammalian bones naturally contain traces of ions, like Mg, which play a vital role in the bone remodeling process. In this way, synthetic hydroxyapatites should include this kind of mineral. In this work, hydroxyapatite added with Mg was synthesized by wet precipitation using（NH;）H;PO;, ca（NO;）;.4 H;O,and Mg（NO;）;.6 H;O（0, 0.032, 0.061, and 0.123 M）. Inductively coupled plasma and X-ray diffraction evidenced the Mg inclusion in the hydroxyapatite lattice in the same levels reported for natural hydroxyapatites. The calcination at 600℃ was performed to remove the reaction by-products, but it also gave rise to physicochemical changes as the coalescence and crystals recrystallization. It produced, in turn, an improvement in the crystalline quality, according to Raman analysis. Despite those physicochemical changes, all the samples remained nanometric according to scanning electron microscopy imaging.

关键词： Magnesium Physicochemical properties calcination Nanometric crystal size Coalescence Recrystallization

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Changes in Human Hair Induced by UV- and Gamma Irradiation

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Advances in Bioscience and Biotechnology 2016年第1期7卷 19-27页

作者： Ervin Palma David gomez Eugene Galicia Viktor Stolc Yuri Griko Evergreen Valley College San Jose CA USA carnegie Melon University Moffett Field CA USA Division of Space Biosciences NASA Ames Research Center Moffett Field CA USA

The effect of UV- and 137Cs gamma radiation on the structural and chemical integrity of human hair was studied to determine the feasibility of using human hair as a non-invasive biomarker of radiation exposure to ionized gamma- and non-ionized UV-radiation. Steady state tryptophan (Trp) fluorescence and chemical analytical methods were used to evaluate the molecular integrity of Trp fluorophores and SH-groups in hair proteins and to assess the radiation induced damage quantitatively. It was found that human hair fibers were progressively damaged by exposure to both UV- and ionized gamma radiation. Damage to the hair was evidenced by a decrease in the fluorescence intensity as a result of observed depletion of the amino acid tryptophan as well as significant reduction in a number of free SH-groups in hair proteins. Hair damage was dose-dependent for exposures between 0 and 10.0 Gy and 0 - 20 J/cm2 of UV-radiation. Additional results demonstrate that hair-fibers exposed to gamma rays, with much higher quantum energy than UV, undergo a smaller extent of changes in Trp fluorescence than when exposed to lower or equal energy of UV-irradiation. The stable Trp fluorophore appears to be extremely sensitive to UV-radiation in contrast to the ionized gamma radiation whose damage is originated from the reaction of free radicals and direct deposition of energy. We conclude that fluorescence spectroscopy represents a useful tool in the quantitative evaluation of the radiation exposure and could also be used for the rapid and non-invasive assessment of radiation dose i.e. biodosimeter. The approach is simple, non-invasive and appears to have considerable potential that enables quantitative evaluation of radiation dose exposure in a single hair fiber.

关键词： Hair Fluorescence Radiation Chemical Integrity

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An essential role of IP3Rs in early T-cell development

An essential role of IP3Rs in early T-cell development

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第九届全国钙信号和细胞功能研讨会

作者： Rafael gomez-Amaro David Stachura David Traver Sylvia M Evans Skaggs School of Pharmacy University of California-San Diego9500 Gilman DriveLa JollaCalifornia92093USA Department of Biology University of California-San Diego9500 Gilman DriveLa JollaCalifornia92093USA

IP3Rs are a family of intracellular ca release channels locating in the membrane of endoplasmic reticulum,which in mammals are encoded by three distinct genes,IP3R1, IP3R2 and *** described here at the first time that conditional deletion of all three types of IP3Rs in hematopoietic stem cells by Tie2-Cre resulted in developmental block from double negative T cell to double positive T cell transition,and the late onset of T-cell acute lymphoblastic leukemia(T-ALL).We revealed that IP3R-mediated ca release downstream of pre-T cell receptors is required for survival,differentiation and

关键词： Cell=T cell Method=confocal imaging Topic=development

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Differences in seed dormancy and germination in amphicarpic legumes:manifold bet-hedging in space and time

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Journal of Plant Ecology 2021年第4期14卷 662-672页

作者： Ricardo Sanchez-Martin Jose Maria gomez Pierre-Olivier Cheptou Rafael Rubio de casas Departamento Ecologia Vegetal Centro de Investigaciones sobre Desertificacion(CIDE-CSIC-UV-GV)Carretera de Moncada-Naquera Km 4546113 MoncadaValenciaSpain Departamento de Ecologia Funcional y Evolutiva Estacion Experimental de Zonas AridasEEZA-CSICCtra.de Sacramento s/nLa Cañada de San Urbano04120 AlmeriaSpain Centre d’Ecologie Fonctionnelle et Evolutive(CEFE) CEFE UMR 5175CNRSUniversite de MontpellierUniversite Paul-Valery7 MontpellierEPHE1919 route de Mende34293 Montpellier Cedex 05France Departamento de Ecologia Universidad de GranadaAvda.de Fuentenueva s/n18071 GranadaSpain

Aims Dispersal through space or time via dormancy is one of the primary processes whereby organisms can influence the environment they *** plants,strong evolutionary correlations are expected between the two kinds of dispersal because both are performed by the seeds and play comparable adaptive *** this paper,we investigated these evolutionary correlations using amphicarpic plants,which simultaneously produce aerial seeds with high spatial dispersal propensity and subterranean seeds that do not *** We investigated the variation in dormancy and germination in aerial and subterranean seeds of two amphicarpic legumes(Vicia amphicarpa *** Lathyrus amphicarpos L.)and two closely related homocarpic taxa(Vicia sativa *** Lathyrus cicera L.)by estimating germination percentages following different combinations of dormancy breaking treatments(*** after-ripening,cold stratification and physical scarification).Important Findings Our results showed complex interactions between spatial and temporal *** after dispersal,aerial seeds were more dormant than their subterranean counterparts,but this trend reversed with after-ripening,as seeds developed physical *** of homocarpic plants germinated at higher percentages than those of their amphicarpic congeners and lost dormancy homogeneously with ***,amphicarpic seeds exhibited varied dormancy strategies modulated by both physiological and physical *** are expected to increase variation in emergence timing,providing multiple levels of diversifying *** strategy might be adaptive under highly unpredictable conditions by enabling plants to rely on historically favorable sites in good seasons without preventing spatial and temporal migration.

关键词： amphicarpy dormancy/colonization trade-off amphicarpic legumes environmental heterogeneity seed dormancy and germination Mediterranean environments

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Amnesia induced by halothane is related with an increase of the 5-HMA and galanin receptors function in the limbic areas of the rat brain.

Amnesia induced by halothane is related with an increase of ...

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第十五届国际药理学大会

作者： Bellido I gomez -Luque A Bellido MV Sanchez de la Cuesta F Pharmacology and Clinical Therapeutics Pharmacology and Clinical Therapeutics.Anaesthesiology Medicine School Nursering School Malaga Pharmacology and Clinical Therapeutics

＜正＞ The effect of halothane’ anaesthesia in the memory and in the rat brain 5 - HT1A and galanin （GAL） receptors were determined. Rats （n = 12. adult male Sprague- Dawley） were anaesthetized with halothane （HAL）（3-5 mmHg in 50% O2- 50 % O2N,30 min）. The memory capacity （8 - arm labyrinth） and brain 5 -HT1A and GAL receptors （ autoradiography techniques with 3H - 8OH - DPAT and 125I - human - porcine galanin） were determined 24 h after. HAL increased the time spent in: the first choice （ + 50 % ）,in the first 1 and 8 accuracy choices （ + 53%,+ 67% ）, and the error choices total number （ + 136% ）. HAL diminished the time spent eating （ - 26% ）. and the accuracy choices total number （ - 44 %） and the first 8 choices accuracy choices number （ -42%）（p＜0. 05）. HAL increased the affinity of GAL receptors in hippocampus and amygdala （Ki increment 82 % and 151 % ）（p ＜ 0.01）. HAL increased the 5 - HT1A receptors affinity of fronto - parietal cortex, hippocampus ca1 and amygdala （Kd reduction - 66% , - 88% and - 63% ） while reduced receptors affinity of dorsal raphe （Kd increment +224%）（p＜0.0l）. Amnesia induced by halothane in rats are linked to an increased neurotransmission of the galanin and the 5 - HT1A receptors in limbic areas.

关键词： halothane brain. 5-HT1A galanin

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Lead Ion Uptake By Sodium Alginate And calcium Alginate Film:A Comparison Study

Lead Ion Uptake By Sodium Alginate And Calcium Alginate Film...

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2012 International Congress on Informatics,Environment,Energy and Applications(IEEA 2012)

作者： Armando gomez Kazimierz Wrobel Katarzyna Wrobel Sato Kazunori Teoh Wah Tzu Department of Chemistry University of GuanajuatoL.de Retana No.5Guanajuato 36000Mexico Department of Environmental Engineering Nagaoka University of TechnologyNagaokaNiigata 9402188Japan

Lead ion (Pb(II)) uptake from aqueous solution by two different alginate compounds was investigated. Sodium alginate (Na-Alg) and calcium alginate (ca-Alg) films were successfully fabricated from the commercially available sodium alginate powder. The Pb(II) uptake performance by Na-Alg and caAlg films was evaluated against time in a 10 mM Pb(II) solution’s batch sorption system. ca-Alg film was consistently showing a better Pb(II) uptake performance as compared to Na-Alg film within the investigated duration. Further evaluation on ca-Alg film was carried out at lower Pb(II) concentrations, i.e. 1 mM and 0.1 mM, the ca-Alg film showed the best performance in 0.1 mM Pb(II) solution at a uptake rate higher than 98%. The Pb(II) uptake performance of ca-Alg film was also compared with that of the conventionally fabricated ca-Alg beads. This result indicates that with the same amount of ca-Alg used, the film shows approximately three time better Pb(II) uptake performance compared to the beads. The results reported herein indicates that ca-Alg compound is an effective and efficient biosorbent, furthermore the Pb(II) uptake performance is influenced by the sample fabrication method as well, i.e. film versus bead, which contributed to the overall surface area.

关键词： alginate films lead uptake sorption

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Volatile organic compound emission and biochemical properties of degraded Ultisols ameliorated by no tillage and liming

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Pedosphere 2020年第5期30卷 597-606页

作者： Laura GIAGNONI Cosimo TAITI Paloma LEON Corrado CosTA Paolo MENESATTI Rafael ESPEJO Clara gomez-PACcaRD Chiquinquira HONTORIA Eduardo VAZQUEZ Marta BENITO Stefano MANCUSO Giancarlo RENELLA Department of Agriculture FoodEnvironment and Forestry(DAGRI)University of FlorenceP.le delle Cascine 18Florence 50144(Italy) Departamento de Production Agraria E.T.S.I.AgrondmicaAlimentaria y de BiosistemasUniversidad Politecnica de MadridAvda.Complutense s/nMadrid 28040(Spain) Consiglio per la Ricerca in Agricoltura e I'Analisi dell'Economia Agraria(CREA)-Unita di Ricerca per ilngegneria Agraria via della Pascolare 16Monterotondo ScaloRM 00015(Italy) Department of Soil Biogeochemistry and Soil Ecology University of BayreuthDr.Hans-Frisch-Strafie 1-3Bayreuth 95448(Germany) University of Padua Department of AgronomyFoodNatural ResourcesAnimals and Environment(DAFNAE)Viale dell'Universita 16Legnaro 35020(Italy)

TTie Ultisols in the Rana de canamero area in Southwest Spain showed aluminum(Al)phytotoxicity,and the clearance of natural vegetation and decades of intensive conventional agriculture caused the deplation of soil organic matter(SOM).Therefore,we studied the long-term effects of no tillage and liming using sugar beet foam(SF)and red gypsum(RG),alone or in combination,on the restoration of Ultisols affected by acidification,Al phytotoxicity,and SOM *** measured the main soil chemical properties,soil microbial biomass,soil enzyme activities involved in carbon,nitrogen,phosphorus,and sulfur mineralization,and the emission of volatile organic compounds(VOCs).The results indicated that liming effectively neutralized the soil acidity in the long term and,in combination with no tillage,significantly increased soil microbial biomass and enzyme ***-three VOCs were detected using the proton transfer reaction-time of flight(PTR-ToF)technique,and both liming and tillage changed the VOC emission *** greatest difference in VOC emission pattern was observed between no-tilled un-amended soils and tilled lime-amended soils,suggesting the activation of different metabolic pathways within the microbial communities of soils under different *** in VOC emission patterns could be attributed to the decomposition of carbohydrates,which were also sustained by the higher enzyme activities in the lime-amended soils.

关键词： carbohydrate decomposition degraded soils long-term field trial soil acidity soil enzyme activities soil microbial biomass soil restoration

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Artificial intelligence for renal cancer:From imaging to histology and beyond

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Asian Journal of Urology 2022年第3期9卷 243-252页

作者： Karl-Friedrich Kowalewski Luisa Egen Chanel E.Fischetti Stefano Puliatti gomez Rivas Juan Mark Taratkin Rivero Belenchon Ines Marie Angela Sidoti Abate Julia Muhlbauer Frederik Wessels Enrico Checcucci Giovanni cacciamani Department of Urology and Urological Surgery University Medical Centre MannheimMannheimGermany Department of Emergency Medicine Brigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA Urology Department University of Modena&Reggio EmiliaModenaItaly ORSI Academy MelleBelgium Department of Urology Hospital Clinico San CarlosMadridSpain Institute for Urology and Reproductive Health Sechenov UniversityMoscowRussia Urology and Nephrology Department Virgen del Rocı´o University HospitalManuel Siurot s/nSevilleSpain Department of Surgery Candiolo Cancer InstituteFPO-IRCCSCandioloTurinItaly USC Institute of Urology University of Southern CaliforniaLos AngelesCAUSA

Artificial intelligence(AI)has made considerable progress within the last decade and is the subject of contemporary *** trend is driven by improved computational abilities and increasing amounts of complex data that allow for new approaches in analysis and *** cell carcinoma(RCC)has a rising incidence since most tumors are now detected at an earlier stage due to improved *** creates considerable challenges as approximately 10%e17%of kidney tumors are designated as benign in histopathological evaluation;however,certain co-morbid populations(the obese and elderly)have an increased peri-interventional *** offers an alternative solution by helping to optimize precision and guidance for diagnostic and therapeutic *** narrative review introduced basic principles and provide a comprehensive overview of current AI techniques for ***,AI applications can be found in any aspect of RCC management including diagnostics,perioperative care,pathology,and *** commonly applied models include neural networks,random forest,support vector machines,and ***,for implementation in daily practice,health care providers need to develop a basic understanding and establish interdisciplinary collaborations in order to standardize datasets,define meaningful endpoints,and unify interpretation.

关键词： Kidney cancer Imaging Technology Artificial intelligence Machine learning

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Nanoengineered Shear-Thinning Hydrogel Barrier for Preventing Postoperative Abdominal Adhesions

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Nano-Micro Letters 2021年第12期13卷 452-467页

作者： Guillermo U.Ruiz-Esparza Xichi Wang Xingcai Zhang Sofia Jimenez-Vazquez Liliana Diaz-gomez Anne-Marie Lavoie Samson Afewerki a.dres AFuentes-Baldemar Roberto Parra-Saldivar Nan Jiang Nasim Annabi Bahram Saleh Ali K.Yetisen Amir Sheikhi Thomas H.Jozefiak Su Ryon Shin Nianguo Dong Ali Khademhosseini Biomaterials Innovation Research Center Division of Engineering in MedicineDepartment of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMA 02139USA Division of Health Sciences and Technology Harvard University-Massachusetts Institute of TechnologyCambridgeMA 02139USA Department of cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022People’s Republic of China School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA School of Engineering and Science Tecnologico de MonterreyCampus MonterreyMonterreyNuevo Leon 64849Mexico School of Medicine and Health Science Campus GuadalajaraZapopanJalisco 45201Mexico West China School of Basic Medical Sciences and Forensic Medicine Sichuan UniversityChengdu 610041China Department of Chemical and Biomolecular Engineering University of CaliforniaLos AngelesLos AngelesCA 90095USA Department of Chemical Engineering Northeastern UniversityBostonMA 02115USA Department of Chemical Engineering Imperial College LondonLondon SW72AZUK Department of Chemical Engineering The Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Biomedical Engineering The Pennsylvania State UniversityUniversity ParkPA 16802USA Terasaki Institute for Biomedical Innovation 11570 W Olympic BlvdLos AngelesCA 90024USA

More than 90%of surgical patients develop postoper-ative adhesions,and the incidence of hospital re-admissions can be as high as 20%.Current adhesion barriers present limited efficacy due to difficulties in application and incompatibility with minimally invasive *** solve thisclinical limitation,we developed an injectable and sprayable shear-thinning hydrogel barrier(STHB)composed of silicate nanoplatelets and poly(ethylene oxide).We optimized this technology to recover mechanical integrity after stress,enabling its delivery though inject-able and sprayable *** also demonstrated limited cell adhesion and cytotoxicity to STHB compositions in *** STHB was then tested in a rodent model of peritoneal injury to determine its e cacy preventing the formation of postoperative *** two weeks,the peritoneal adhesion index was used as a scoring method to determine the formation of postoperative adhesions,and STHB formulations presented superior e cacy compared to a commercially available adhesion *** and immunohistochemical examination showed reduced adhesion formation and minimal immune infiltration in STHB *** technology demonstrated increased e cacy,ease of use in complex anatomies,and compatibility with di erent delivery methods,providing a robust universal platform to prevent postoperative adhesions in a wide range of surgical interventions.

关键词： Postoperative adhesions Shear-thinning hydrogel Silicate nanoplatelets Nanotechnology Nanomedicine

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