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Micro-Tuber Production in Diploid and Tetraploid Potato after Gamma Irradiation of in Vitro Cuttings for Mutation Induction

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American Journal of Plant Sciences 2016年第14期7卷 1871-1887页

作者： Souleymane Bado Margit Laimer Ndiogou Gueye Ndeye Fatou deme enoch Sapey Abdelbagi Mukhtar Ali Ghanim Vivian Carol Blok Brian Peter Forster Plant Breeding and Genetics Laboratory (PBGL) Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture FAO/IAEA Laboratories Seibersdorf Austria Plant Biotechnology Unit Department of Biotechnology University of Natural Resources and Life Sciences Vienna Austria Biotechnology Laboratory of Mushrooms Department of Plant Biology Faculty of Sciences & Techniques University of Cheikh Anta Diop Dakar Fann Sénégal Council for Scientific and Industrial Research (CSIR)-Oil Palm Research Institute (OPRI) Kade Ghana The James Hutton Institute Errol Rd Invergowrie Dundee UK BioHybrids International Ltd. Reading UK

Micro-tubers are important propagules in potato breeding and potato production, and they are also dormant and easily transported and therefore good targets for mutation induction in potato mutation breeding. A prerequisite for mutation breeding is to determine optimal mutation treatments. Therefore, radio-sensitivity tests of a tetraploid and a diploid potato to gamma irradiation were undertaken. effects of different gamma sources on radio-activity were also studied. In vitro potato cuttings were gamma irradiated using a wide dose range (0, 3, 6, 9, 12, 15 and 20 Gy). The irradiated cuttings were then cultured to induce micro-tubers directly in vitro. Micro-tuber morphotypes were assessed after irradiation of cuttings using three gamma sources with emission activities of 1.8, 7.07 and 139 Gy/min. The diploid species (Solanum verrucosum) was more radio-sensitive than the tetraploid cultivar Desirée (Solanum tuberosum). Gamma dose rates had significant influences on subsequent micro-tuber production at various mutant generations. effects included reductions in the number, size and weight of micro-tubers produced. Gamma dose was more lethal for the diploid potato genotype and micro-tubers produced were small compared to those produced by the tetraploid genotype after irradiation. Different treatments are recommended for diploid and tetraploid potato irradiation in producing large mutant micro-tuber populations. The mutant micro-tuber populations may then be screened for interesting mutations/trait for both genetics and plant breeding purposes.

关键词： Potato in Vitro Cuttings Micro-Tubers Mutation Induction Gamma Irradiation Mutant Populations Recommended Dose Rates

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From Surgery to Hematology: Fanconi Anemia about a Case of a Senegalese Child

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Open Journal of Pediatrics 2020年第4期10卷 635-641页

作者： A. Kane I. deme/ly G. Diagne D. A. Mbaye M. Fattah A. Sow N. F. Sow A. M. Coundoul S. Sow K. Bop P. M. Faye A. L. Fall O. Ndiaye Department of Pediatrics of the National Children’s Center Albert Royer Dakar Senegal Department of Pediatrics of the National Hospital Center Abass NDAO Dakar Senegal Department of Pediatrics of the University Hospital Center Aristide Le Dantec Dakar Senegal

Fanconi anaemia (FA) is a rare genetic abnormality. Most of FA reported from the sub-Saharan population came from southern Africa, with many patients linked to a mutation in the FANC G gene. Classic triads of this disorder are: a varied malformation, bone marrow failure, and short stature. This disorder is also associated with predisposition to malignancies. These multiple manifestations, sometimes not uniform, often cause diagnostic delay. We here report a 13-year-old Senegalese boy with FA. He was followed up for polydactyly-repair surgery. Importantly, pre-operative blood check-up revealed severe anaemia, which prompted us to perform bone marrow aspiration: examination revealed dysmyelopoiesis. Being triggered by this, systematic examinations were performed, which revealed other signs indicative of FA;i.e., radial spine abnormalities, triangular face, etc. The diagnosis of FA was strongly suggested, which prompted genetic examination. The chromosomal breakage test detected cellular hypersensitivity to DNA, which confirmed the diagnosis. He is receiving transfusion support, and androgens use is being considered. This case highlights the importance of preoperativee="font-family:Verdana;"> examination. Physicians must be aware that FA, although its incidence is low, can be hidden behind infantile anaemia.

关键词： Aplastic Anaemia West Africa Paediatric Surgery Malformations

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卧龙自然保护区人类活动与大熊猫(Ailuropoda melanoleuca)空间利用特征分析

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四川林业科技 2019年第5期40卷 60-65页

作者：的么罗英王晓张晋东西华师范大学生命科学学院

目前,关于野生动物与人类的空间共存机制主要存在3种假设模式:即毗邻但空间不重叠;共享局域空间,但活动区域分开;共存在小尺度空间。明确人类与野生动物的空间利用模式,能为野生动物的保护管理提出科学参考依据。为此,本研究利用红外相... 详细信息

目前,关于野生动物与人类的空间共存机制主要存在3种假设模式:即毗邻但空间不重叠;共享局域空间,但活动区域分开;共存在小尺度空间。明确人类与野生动物的空间利用模式,能为野生动物的保护管理提出科学参考依据。为此,本研究利用红外相机在卧龙自然保护区大熊猫(Ailuropoda melanoleuca)的集中分布区域同时监测大熊猫及人类活动,通过分析大熊猫和人类活动的空间分布特征,探讨大熊猫与人类的共存机制。结果表明:(1)研究初期(2011.11—2013.04)人类活动月相对丰富度较低,此时该区域内监测到的大熊猫月相对丰富度较高。但从2013年5月起人类活动强度骤增且此后一直稳定在较高水平,导致大熊猫的月相对丰富度急剧降低。(2)虽然在关键的限制性因子(如水源等)的利用上大熊猫与人类活动的区域存在重叠,但总体而言,人类活动频繁出现的区域大熊猫的出现频次明显减少。结合人类与野生动物共存的3种机制分析得出大熊猫与人类活动之间的共存模式为共享局域空间,但活动区域分开。人类活动在一定程度上制约着野生动物的生存和发展,保护区应全面考虑人类干扰活动对大熊猫的影响,制定可持续发展的政策,同时,减少人类与大熊猫对空间和资源的竞争。

关键词：共存机制大熊猫人类活动红外相机卧龙自然保护区

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全程导乐助产护理对足月妊娠初产妇分娩妊娠结局及心理状态的影响

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智慧健康 2021年第27期7卷 82-84页

作者：邓飞娥广东省人民医院珠海医院妇产科广东珠海519000

目的探讨全程导乐助产护理对足月妊娠初产妇分娩妊娠结局及心理状态的影响。方法选取2018年4月至2019年4月在广东省人民医院珠海医院生产的84例足月妊娠初产妇,按照入院时间分为对照组(42例,常规护理)与观察组(42例,全程导乐助产护理)... 详细信息

目的探讨全程导乐助产护理对足月妊娠初产妇分娩妊娠结局及心理状态的影响。方法选取2018年4月至2019年4月在广东省人民医院珠海医院生产的84例足月妊娠初产妇,按照入院时间分为对照组(42例,常规护理)与观察组(42例,全程导乐助产护理),比较两组初产妇护理效果。结果干预后,观察组焦虑自评量表评分(SAS)、抑郁自评量表评分(SDS)均显著低于对照组,差异有统计学意义(P<0.05);观察组第一产程、第二产程、第三产程及产后的VAS评分均低于对照组,差异有统计学意义(P<0.05);观察组不良妊娠结局发生率显著低于对照组,差异有统计学意义(P<0.05);观察组初产妇满意度高于对照组,差异有统计学意义(P<0.05)。结论在足月妊娠初产妇中实施全程导乐助产护理模式,有助于缓解负面心理,降低疼痛程度,改善妊娠结局及初产妇的满意度。

关键词：初产妇全程导乐助产护理负面情绪妊娠结局

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