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Cytotoxicity and non-specific cellular uptake of b.re and surface-modified upconversion nanoparticles in human skin cells

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Nano Research 2015年第5期8卷 1546-1562页

作者： Anna E. Guller Alia N. Generalova Elena V. Petersen Andrey V. Nechaev Inna A. Trusova Nikolay N. Landyshev Annemarie Nadort Ekaterina A. Greb.nik Sergey M. Deyev a.a.oly b. shekhter Andrei V. Zvyagin Macquarie University Australia I.M. Sechenov First Moscow State Medical University Russia Shemyakin-Ovchinnikov Institute of b.oorganic Chemistry Russian Academy of Sciences Russia Nizhny Novgorod State University Russia Moscow Institute of Physics and Technology Russia Moscow State University of Fine Chemical Technologies Russia University of Amsterdam the Netherlands

The cytotoxicity and non-specific cellular uptake of the most popular composition of upconversion nanoparticle （UCNP）, NaYF4：Yb.3＋：Er^3＋, is reported using normal human skin cells, including dermal fib.ob.asts and immortalized human epidermal linear keratinocytes （HaCaT）. A new hydrophilization reaction of as-synthesized UCNPs b.sed on tetramethylammonium hydroxide （TMAH） enab.ed evaluation of the intrinsic cytotoxicity of b.re UCNPs. The cytotoxicity effects of the UCNP surface-coating and polystyrene host were investigated over the concentration range 62.5-125 μg/mL with 24-h incub.tion, using a MTT test and optical microscopy. The fib.ob.ast viab.lity was not compromised b. UCNPs, whereas the viab.lity of keratinocytes varied from 52% ± 4% to 100% ± 10% than the control group, depending on the surface modification. b.re UCNPs reduced the keratinocyte viab.lity to 76% ± 3%, while exhib.ting profound non-specific cellular uptake. Hydrophilic poly（D,L-lactide）- and poly（maleic anhydride-alt-l-octadecene）-coated UCNPs were found to b. least cytotoxic among the polymer-coated UCNPs, and were readily internalized b. human skin cells. Polystyrene microb.ads impregnated with UCNPs remained nontoxic. Surprisingly, no correlation was found b.tween UCNP cytotoxicity and the internalization level in cells, although the latter ranged b.oadly from 0.03% to 59%, b.nchmarked against 100% uptake level of TMAH-UCNPs.

关键词： nanoparticle upconversion surface modification b.ocompatib.lity cytotoxicity human skin

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