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Switching On/Off of Guide RNA by Photoinduced Strand Displacement for Functional Control of CRISPR/Cas9

CCS Chemistry 2024年第5期6卷 1338-1351页

作者： Yang wang Chao-Qun Wu Qiu-Long Zhang Ming Shao Yan Liu Liang-Liang wang zun-yun wang Juan Du Liang Xu Central Laboratory Department of the Second Affiliated Hospital School of MedicineThe Chinese University of Hong KongShenzhen&Longgang District People’s Hospital of ShenzhenShenzhen 518172 Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringShenzhen University Medical SchoolShenzhen 518060 MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510275 School of Pharmacy and Medical Technology Putian UniversityPutian 351100

Functional control of CRISPR/Cas9 is essential for precise gene *** engineering of guide RNA(gRNA)provides diverse approaches for conditional control of CRISPR/Cas9 function with a variety of chemical reactive ***,previous investigations into chemically engineering gRNA only unidirectionally regulated the CRISPR/Cas9 function via stimuli-induced caging/decaging ***,we propose a combinatory strategy to engineer the dynamics of gRNA in which photocontrolled strand-displacement reactions coupled with sequence designs of gRNA can achieve lightinduced switching-on/off control of CRISPR/Cas9 *** analysis and cellular gene regulation indicate this approach is capable of both activating and deactivating CRISPR/Cas9 activities using light ***,photocontrolled multiplex modulations of gene expression for opposite regulatory effects have also been achieved simultaneously under the same cellular *** work establishes an essential principle for construction of stimuli-induced switching-on/off modulations of gRNA that can greatly enrich the versatility of conditional control for a variety of CRISPR/Cas9-based applications.

关键词： modified oligonucleotides gene manipulation optical control CRISPR/Cas9 strand displacement

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Accuracy of numerical relativity waveforms with respect to space-based gravitational wave detectors

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Communications in Theoretical Physics 2024年第1期76卷 82-95页

作者： zun wang Junjie Zhao Zhoujian Cao Institute for Frontiers in Astronomy and Astrophysics Beijing Normal UniversityBeijing 102206China Department of Astronomy Beijing Normal UniversityBeijing 100875China School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUCASHangzhou 310024China

As with the laser interferometer gravitational-wave observatory(LIGO),the matched filtering technique will be critical to the data analysis of gravitational wave detection by space-based detectors,including LISA,Taiji and *** templates are the basis for such matched filtering *** construct ready-to-use waveform templates,numerical relativity waveforms are a starting ***,the accuracy issue of numerical relativity waveforms is critically *** are many investigations regarding this issue with respect to *** unfortunately there are few results on this issue with respect to space-based *** current paper investigates this *** results indicate that the existing numerical relativity waveforms are as accurate as 99%with respect to space-based detectors,including LISA,Taiji and *** an accuracy level is comparable to that with respect to LIGO.

关键词： numerical relativity gravitational wave binary black hole

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Strong evidence for latitudinal diversity gradient in mosses across the world

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Plant Diversity 2024年第4期46卷 537-541页

作者： Hong Qian zun Dai Jian wang Research and Collections Center Illinois State Museum1011 East Ash StreetSpringfieldIL 62703USA Bryology Laboratory School of Life SciencesEast China Normal UniversityShanghai 200241China Shanghai Institute of Eco-Chongming(SIEC) 3663 Northern Zhongshan RoadShanghai 200062China

Species richness generally decreases with increasing latitude,a biodiversity gradient that has long been considered as one of the few laws in *** latitudinal diversity gradient has been observed in many major groups of *** plants,the latitudinal diversity gradient has been observed in vascular plants,angiosperms,ferns,and ***,a conspicuous latitudinal diversity gradient in mosses at a global or continental scale has not been observed until ***,we analyze a comprehensive data set including moss species in each band of 20° in latitude *** results show that moss species richness decreases strongly with increasing latitude,regardless of whether the globe is considered as a whole or different longitudinal segments(e.g.,Old World versus New World) are considered *** result holds when variation in area size among latitudinal bands is taken into ***'s correlation coefficient between latitude and species richness is-0.99 for both the Northern and Southern *** bryophytes are an extant lineage of early land plants and because mosses not only include most of extant species of bryophytes but also are important constituents of most terrestrial ecosystems,understanding geographic patterns of mosses is particularly important The finding of our study fills a critical knowledge gap.

关键词： Bryophyte Latitudinal diversity gradient Moss Species density Species diversity

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Aerodynamic Features of High-Speed Maglev Trains with Different Marshaling Lengths Running on a Viaduct under Crosswinds

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Computer Modeling in Engineering & Sciences 2024年第7期140卷 975-996页

作者： zun-Di Huang Zhen-Bin Zhou Ning Chang Zheng-Wei Chen Su-Mei wang School of Rail Transportation Wuyi UniversityJiangmen529020China National Rail Transit Electrification and Automation Engineering Technology Research Center(Hong Kong Branch) The Hong Kong Polytechnic UniversityHong Kong999077China Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHong Kong999077China

The safety and stability of high-speed maglev trains traveling on viaducts in crosswinds critically depend on their aerodynamic ***,this paper uses an improved delayed detached eddy simulation(IDDES)method to investigate the aerodynamic features of high-speed maglev trains with different marshaling lengths under *** effects of marshaling lengths(varying from 3-car to 8-car groups)on the train’s aerodynamic performance,surface pressure,and the flow field surrounding the train were investigated using the three-dimensional unsteady compressible Navier-Stokes(N-S)*** results showed that the marshaling lengths had minimal influence on the aerodynamic performance of the head and middle ***,the marshaling lengths are negatively correlated with the time-average side force coefficient(CS)and time-average lift force coefficient(Cl)of the tail *** to the tail car of the 3-car groups,the CS and Cl fell by 27.77%and 18.29%,respectively,for the tail car of the 8-car *** is essential to pay more attention to the operational safety of the head car,as it exhibits the highest time average ***,the mean pressure difference between the two sides of the tail car body increased with the marshaling lengths,and the side force direction on the tail car was opposite to that of the head and middle ***,the turbulent kinetic energy of the wake structure on the windward side quickly decreased as marshaling lengths increased.

关键词： High-speed maglev train marshaling lengths crosswinds aerodynamic features

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Millimeter-wave PA design techniques in ISSCC 2024

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Journal of Semiconductors 2024年第4期45卷 16-18页

作者： yun wang Hongtao Xu State Key Laboratory of Integrated Chips and Systems School of MicroelectronicsFudan UniversityShanghai 201203China

Millimeter-wave systems with high integration level have been rapidly developed to enable modern wireless communication,sensing,and *** millimeter-wave power amplifier(PA)is one of the most challenging and critical components in a millimeter-wave transceiver,it is the last active stage in a transmitter amplifying spectrally efficient complex modulation signal for radiation with high output power.

关键词： millimeter Millimeter ISSCC

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Regulating depletion region enables highly efficient and stable perovskite solar cells

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Science China Chemistry 2024年第9期67卷 2783-2784页

作者： Chun-yun wang Michael Saliba Institute for Photovoltaics(ipv) University of Stuttgart70569 StuttgartGermany IEK5-Photovoltaics Forschungszentrum Jülich52425 JülichGermany

Perovskite solar cells(PSCs)have made impressive strides in recent years with the power conversion efficiency(PCE)now exceeding 26%[1],which is comparable to established technologies such as ***,the ion migration in perovskites causes undesirable hysteresis and *** affects the long-term operational stability of PSCs,hampering a possible industrialization.

关键词： perovskite depletion exceeding

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Gria:an efficient deterministic concurrency control protocol

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Frontiers of Computer Science 2024年第4期18卷 25-37页

作者： Xinyuan wang yun PENG Hejiao HUANG School of Computer Science and Technology Harbin Institute of TechnologyShenzhen 518055China Institute of Artificial Intelligence and Blockchain Guangzhou UniversityGuangzhou 510006China

Deterministic databases are able to reduce coordination costs in a *** property has fostered a significant interest in the design of efficient deterministic concurrency control ***,the state-of-the-art deterministic concurrency control protocol Aria has three ***,it is impractical to configure a suitable batch size when the read-write set is ***,Aria running in low-concurrency scenarios,e.g.,a single-thread scenario,suffers from the same conflicts as running in high-concurrency ***,the single-version schema brings write-after-write *** address these issues,we propose Gria,an efficient deterministic concurrency control *** has the following ***,the batch size of Gria is ***,Gria’s conflict probability in low-concurrency scenarios is lower than that in high-concurrency ***,Gria has no write-after-write conflicts by adopting a multi-version *** further reduce conflicts,we propose two optimizations:a reordering mechanism as well as a rechecking *** evaluation result on two popular benchmarks shows that Gria outperforms Aria by 13x.

关键词： deterministic concurrency control transaction processing

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Dynamic Event-Triggered Quadratic Nonfragile Filtering for Non-Gaussian Systems:Tackling Multiplicative Noises and Missing Measurements

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1127-1138页

作者： Shaoying wang Zidong wang Hongli Dong yun Chen Guoping Lu College of Science Shandong University of AeronauticsBinzhou 256603China College of Electrical Engineering and Automation Shandong University of Science and TechnologyQingdao 266590 Department of Computer Science Brunel University LondonUxbridge UB83PHUK Artificial Intelligence Energy Research Institute Northeast Petroleum UniversityDaqing 163318 Heilongjiang Provincial Key Laboratory of Networking and Intelligent Control Northeast Petroleum UniversityDaqing 163318China School of Automation Hangzhou Dianzi UniversityHangzhou 310018China School of Electrical Engineering Nantong UniversityNantong 226019China

This paper focuses on the quadratic nonfragile filtering problem for linear non-Gaussian systems under multiplicative noises,multiple missing measurements as well as the dynamic event-triggered transmission *** multiple missing measurements are characterized through random variables that obey some given probability distributions,and thresholds of the dynamic event-triggered scheme can be adjusted dynamically via an auxiliary *** attention is concentrated on designing a dynamic event-triggered quadratic nonfragile filter in the well-known minimum-variance *** this end,the original system is first augmented by stacking its state/measurement vectors together with second-order Kronecker powers,thus the original design issue is reformulated as that of the augmented ***,we analyze statistical properties of augmented noises as well as high-order moments of certain random *** the aid of two well-defined matrix difference equations,we not only obtain upper bounds on filtering error covariances,but also minimize those bounds via carefully designing gain ***,an example is presented to explain the effectiveness of this newly established quadratic filtering algorithm.

关键词： filtering quadratic bounds

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Synergistic effect of gradient Zn content and multiscale particles on the mechanical properties of Al-Zn-Mg-Cu alloys with coupling distribution of coarse-fine grains

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International Journal of Minerals,Metallurgy and Materials 2024年第6期31卷 1392-1405页

作者： Liangliang Yuan Mingxing Guo Yi wang yun wang Linzhong Zhuang Institute of Effectiveness Evaluation of Flying Vehicle Beijing 100085China State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China Beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing 100083China Beijing Laboratory of Metallic Materials and Processing for Modern Transportation University of Science and Technology BeijingBeijing 100083China

This study investigated the influence of graded Zn content on the evolution of precipitated and iron-rich phases and grain struc-ture of the alloys,designed and developed the Al–8.0Zn–1.5Mg–1.5Cu–0.2Fe(wt%)alloy with high strength and *** the increase of Zn content,forming the coupling distribution of multiscale precipitates and iron-rich phases with a reasonable matching ratio and dispersion distribution characteristics is *** phenomenon induces the formation of cell-like structures with alternate distribu-tion of coarse and fine grains,and the average plasticity–strain ratio(characterizing the formability)of the pre-aged alloy with a high strength is up to *** reveal the evolution and influence mechanisms of multiscale second-phase particles and the corresponding high formability mechanism of the *** developed coupling control process exhibits considerable potential,revealing remarkable improvements in the room temperature formability of high-strength Al–Zn–Mg–Cu alloys.

关键词： Al–Zn–Mg–Cu alloy iron-rich phase high formability microstructure mechanisms

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Influence of misting system on the thermal environment and thermal comfort of seated people in semi-outdoor space in Xi’an,China

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Frontiers of Architectural Research 2024年第3期13卷 668-681页

作者： Xu Xie Zhen Sun Xi Zhu Shengkai Zhao zun wang Yongchao Zhai College of Architecture Xi’an University of Architecture and TechnologyXi’an 710055China State Key Laboratory of Green Building Xi’an University of Architecture and TechnologyXi’an 710055China

In summer,factors such as solar radiation could make semi-outdoor spaces uncomfortable,and the use of misting systems for evaporative cooling is a low-energy improvement *** investigate the impact of misting system on the thermal environment and thermal comfort of seated people improvement,a field study was conducted at a semi-outdoor cafeteria in Xi’*** results indicated that the misting system was capable of reducing the ambient air temperature by 2.4-4.9℃and increasing the relative humidity by 17.1%-17.8%.Participants in misting condition reported lower thermal sensation and higher thermal acceptability,thermal preference,humidity sensation,humidity preference and humidity *** no misting condition,the upper SET*limit acceptable to 80%of the population was 28.5℃.However,in the misting condition,the acceptable percentage of participants in each SET)interval was more than 90%.When SET)was 22.1-23.7℃,the improvement in human thermal comfort through misting system was not *** SET)was 23.7-28.5℃,the misting system significantly improve human thermal ***,two control temperature thresholds of the misting system were provided according to the relationship between 80%,the maximum(89.3%)acceptable percentage and the air temperature,which were 30.1℃and 26.5℃,respectively.

关键词： Misting system Thermal environment Thermal response Acceptable temperature Control strategy

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