检索结果-南通市图书馆

Chinese Journal of Chemical Engineering 2024年第1期65卷 276-285页

作者： Yin wu Yan Sun Department of Biochemical Engineering School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology(Ministry of Education)Tianjin UniversityTianjin 300350China

Biocatalysis in organic solvents(OSs)has numerous important applications,but native enzymes in OSs often exhibit limited catalytic ***,we proposed a computation-aided surface charge engineering strategy to improve the catalytic performance of haloalkane dehalogenase DhaA in OSs based on the energetic analysis of substrate binding to the DhaA *** variants with enhanced OS resistance were obtained by replacing negative charged residues on the surface with positive charged residue(Arg).Particularly,a four-substitution variant E16R/E93R/E121R/E257R exhibited the best catalytic performance(five-fold improvement in OS resistance and seven-fold half-life increase in 40%(vol)dimethylsulfoxide).As a result,the overall catalytic performance of the variant could be at least 26 times higher than the wild-type *** spectroscopy and molecular dynamics simulation studies revealed that the residue substitution mainly enhanced OS resistance from four aspects:(a)improved the overall structural stability,(b)increased the hydrophobicity of the local microenvironment around the catalytic triad,(c)enriched the hydrophobic substrate around the enzyme molecule,and(d)lowered the contact frequency between OS molecules and the catalytic *** findings validate that computationaided surface charge engineering is an effective and ingenious rational strategy for tailoring enzyme performance in OSs.

关键词： Surface charge engineering Organic solvent resistance Molecular dynamics simulation Haloalkane dehalogenase

Dual-ion carrier storage through Mg^(2+) addition for high-energy and long-life zinc-ion hybrid capacitor

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2024年第1期31卷 179-185页

作者： Junjie Zhang Xiang wu School of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China

Cation additives can efficiently enhance the total electrochemical capabilities of zinc-ion hybrid capacitors (ZHCs).However their energy storage mechanisms in zinc-based systems are still under ***,we modulate the electrolyte and achieve dual-ion storage by adding magnesium *** we assemble several Zn//activated carbon devices with different electrolyte concentrations and investigate their electrochemical reaction dynamic *** zinc-ion capacitor with Mg^(2+)mixed solution delivers 82 mAh·g^(-1)capacity at 1 A·g^(-1) and maintains 91%of the original capacitance after 10000 *** is superior to the other assembled zinc-ion devices in single-component *** finding demonstrates that the double-ion storage mechanism enables the superior rate performance and long cycle lifetime of ZHCs.

关键词： zinc-ion hybrid capacitor MgSO_(4) electrolyte rate performance storage mechanism

两株海洋真菌的鉴定及其次级代谢产物抑制烟草花叶病毒及抗肿瘤活性(英文)

在线全文

学校读者我要写书评

暂无评论

微生物学报 2009年第9期49卷 1240-1246页

作者：沈硕李玮欧阳明安吴祖建林奇英谢联辉福建农林大学生物农药与化学生物学教育部重点实验室福建农林大学植物病毒研究所福州350002

【目的】通过对2株活性海洋真菌发酵产物提取物抑制烟草花叶病毒和抗肿瘤活性进行研究,为进一步得到活性纯品化合物作为抗病毒及抗肿瘤的先导化合物奠定基础。【方法】菌株发酵产物的粗提物是通过甲醇浸取并在真空条件下蒸干得到的。粗... 详细信息

【目的】通过对2株活性海洋真菌发酵产物提取物抑制烟草花叶病毒和抗肿瘤活性进行研究,为进一步得到活性纯品化合物作为抗病毒及抗肿瘤的先导化合物奠定基础。【方法】菌株发酵产物的粗提物是通过甲醇浸取并在真空条件下蒸干得到的。粗提物中溶于水的部分为水溶性部分,不溶于水的部分为脂溶性部分。通过间接酶联免疫法检测样品抑制烟草花叶病毒的活性,通过四甲基偶氮唑盐微量酶反应比色法(MTT法)检测样品抗肿瘤活性,通过形态及ITSrDNA序列法进行菌株鉴定。【结果】两株海洋真菌抑制烟草花叶病毒活性和抗肿瘤的活性均较高。分子鉴定结果显示,两株真菌分别与Penicillium oxalicum和Neosartorya fischeri的同源性极高。菌株0312F1发酵液的水溶性部分具有抗病毒及抗肿瘤活性,菌株1008F1发酵液的脂溶性部分具有抑制烟草花叶病毒活性,而水溶性部分具有抗肿瘤活性。【结论】菌株0312F1和菌株1008F1发酵液的提取物抑制烟草花叶病毒的活性部位不同,而抗肿瘤活性部位相同。菌株0312F1发酵液提取物的水溶性活性部位对肝癌细胞BEL-7404的抑制效果比对胃癌细胞SGC-7901的抑制效果明显,而菌株1008F1发酵液提取物的水溶性活性部位对胃癌细胞SGC-7901的抑制效果比对肝癌细胞BEL-7404的抑制效果明显。

关键词：海洋真菌抑制活性烟草花叶病毒(Tobacco Mosaic Virus,TMV) 抗肿瘤鉴定

Partially-Observed Maximum Principle for Backward Stochastic Differential Delay Equations

在线全文

学校读者我要写书评

暂无评论

IEEE/CAA Journal of Automatica Sinica 2024年第6期11卷 1524-1526页

作者： Shuang wu Department of Applied Mathematics China University of PetroleumQingdao 266580China

Dear Editor,This letter investigates a partially-observed optimal control problem for backward stochastic differential delay equations(BSDDEs).By utilizing Girsanov’s theory and convex variational method,we obtain a maximum principle on the assumption that the state equation contains time delay and the control domain is *** adjoint processes can be represented as the solutions of certain time-advanced stochastic differential equations in finite-dimensional *** backward stochastic differential equation(BSDE)was first introduced by Bismut in[1],while general BSDE was given by Pardoux and Peng[2].Since then,the theory of BSDEs developed *** corresponding optimal control problems,whose states are driven by BSDEs,have also been widely studied by some authors,see[3]-[5].

关键词： stochastic backward convex

Recent advances and key perspectives of in-situ studies for oxygen evolution reaction in water electrolysis

在线全文

学校读者我要写书评

暂无评论

Green Energy & Environment 2024年第10期9卷 1497-1517页

作者： Yi Wang Zichen Xu Xianhong wu Zhong-Shuai wu State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Shijingshan District Dalian National Laboratory for Clean Energy Chinese Academy of Sciences

Electricity-driven water splitting to produce hydrogen is one of the most efficient ways to alleviate energy crisis and environmental pollution problems, in which the anodic oxygen evolution reaction(OER) is the key half-reaction of performance-limiting in water splitting. Given the complicated reaction process and surface reconstruction of the involved catalysts under actual working conditions, unraveling the real active sites, probing multiple reaction intermediates and clarifying catalytic pathways through in-situ characterization techniques and theoretical calculations are essential. In this review, we summarize the recent advancements in understanding the catalytic process, unlocking the water oxidation active phase and elucidating catalytic mechanism of water oxidation by various in-situ characterization techniques. Firstly, we introduce conventionally proposed traditional catalytic mechanisms and novel evolutionary mechanisms of OER, and highlight the significance of optimal catalytic pathways and intrinsic stability. Next, we provide a comprehensive overview of the fundamental working principles,different detection modes, applicable scenarios, and limitations associated with the in-situ characterization techniques. Further, we exemplified the in-situ studies and discussed phase transition detection, visualization of speciation evolution, electronic structure tracking, observation of reaction active intermediates, and monitoring of catalytic products, as well as establishing catalytic structure–activity relationships and catalytic mechanism. Finally, the key challenges and future perspectives for demystifying the water oxidation process are briefly proposed.

关键词： In-situ studies Water splitting Oxygen evolution reaction Catalytic mechanism

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

灰飞虱酵母双杂交cDNA文库的构建及分析

植物保护 2011年第1期37卷 19-23页

作者：肖冬来邓慧颖谢荔岩吴祖建谢联辉福建农林大学植物病毒研究所福建省植物病毒学重点实验室福州350002

以灰飞虱（Laodlphax striatellus Fallen）mRNA为起始模板，利用Gateway技术构建了灰飞虱酵母双杂交cDNA文库。经过检测表明：构建的初级cDNA文库的库容量为1．85×10 7cfu；扩增文库滴度为7．7×10 8cfu／mL，重组率约为97％；扩增文... 详细信息

以灰飞虱（Laodlphax striatellus Fallen）mRNA为起始模板，利用Gateway技术构建了灰飞虱酵母双杂交cDNA文库。经过检测表明：构建的初级cDNA文库的库容量为1．85×10 7cfu；扩增文库滴度为7．7×10 8cfu／mL，重组率约为97％；扩增文库插入片段主要集中在1000-1500bp之间。随机挑取10个克隆，经测序与GenBank数据库比对结果显示7个克隆具有同源序列，其中L2、L9为已公布的灰飞虱序列。灰飞虱酵母双杂交cDNA文库的构建为克隆全长目的基因及研究灰飞虱与其传播的水稻病毒间的互作奠定了基础。

关键词：灰飞虱酵母双杂交 Gateway技术 cDNA文库

Enhancing performance of low-temperature processed CsPbI2Br all-inorganic perovskite solar cells using polyethylene oxide-modified TiO_(2)

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2024年第4期31卷 786-794页

作者： Xu Zhao Naitao Gao Shengcheng wu Shaozhen Li Sujuan wu School of Electrical and Electronic Engineering Wuhan Polytechnic UniversityWuhan 430023China Institute for Advanced Materials and Guangdong Provincial Key Laboratory of Quantum Engineering and Quantum Materials South China Academy of Advanced OptoelectronicsSouth China Normal UniversityGuangzhou 510006China

CsPbX_(3)-based(X=I,Br,Cl)inorganic perovskite solar cells(PSCs)prepared by low-temperature process have attracted much attention because of their low cost and excellent thermal ***,the high trap state density and serious charge recombination between low-temperature processed TiO_(2)film and inorganic perovskite layer interface seriously restrict the performance of all-inorganic *** a thin polyethylene oxide(PEO)layer is employed to modify TiO_(2)film to passivate traps and promote carrier *** impacts of PEO layer on microstructure and photoelectric characteristics of TiO_(2)film and related devices are systematically *** results suggest that PEO modification can reduce the surface roughness of TiO_(2)film,decrease its average surface potential,and passivate trap *** optimal conditions,the champion efficiency of CsPbI_(2)Br PSCs with PEO-modified TiO_(2)(PEO-PSCs)has been improved to 11.24%from 9.03%of reference ***,the hysteresis behavior and charge recombination have been suppressed in PEO-PSCs.

关键词： polyethylene oxide-modified TiO_(2) film low-temperature process CsPbI_(2)Br-based all-inorganic perovskite solar cells photo-voltaic performance

草莓轻型黄边病毒中国分离物全基因组序列测定和分析

在线全文

学校读者我要写书评

暂无评论

病毒学报 2022年第5期38卷 1187-1194页

作者：江山陈道叶立乐张洁吴祖建丁新伦福建农林大学植物病毒研究所福建省植物病毒重点实验室闽台作物有害生物生态防控国家重点实验室福州350002

草莓轻型黄边病毒(Strawberry mild yellow edge virus,SMYEV)是引起我国草莓病毒病害的重要病毒之一,我们测定了共侵染草莓植株的2个SMYEV中国分离物FJ1和FJ2的全基因组序列。FJ1和FJ2分离物基因组全长均为5971 nt,包含5个开放阅读框(O... 详细信息

草莓轻型黄边病毒(Strawberry mild yellow edge virus,SMYEV)是引起我国草莓病毒病害的重要病毒之一,我们测定了共侵染草莓植株的2个SMYEV中国分离物FJ1和FJ2的全基因组序列。FJ1和FJ2分离物基因组全长均为5971 nt,包含5个开放阅读框(ORF),其中,FJ2分离物ORF4编码蛋白C端缺乏7 aa。SMYEV分离物FJ1和FJ2的基因组核苷酸序列一致性为89.8%,两者和其他SMYEV分离物的基因组核苷酸序列一致性分别为83.7%~89.8%和84.1%~90.0%;基于CP基因的系统进化分析表明,SMYEV分离物分为2个大组群4个亚群,FJ1和FJ2分别归属不同的组,两者具有较远的亲缘关系,其中FJ1与中国分离物sy01和sy04、日本分离物T1以及韩国分离物KNS1亲缘关系最近,FJ2与中国分离物sy02、加拿大一株分离物PEI113_12以及日本分离物T2亲缘关系最近。RDP4重组分析表明,FJ1和FJ2均存在潜在的重组事件。本研究首次报道了我国SMYEV基因组全长序列,为该病毒在我国的侵染流行、检测和防控提供了理论基础。

关键词：草莓草莓轻型黄边病毒基因组全长序列分析

2019—2020年河南省小麦茎基腐病病原菌鉴定及致病力测定

在线全文

学校读者我要写书评

暂无评论

植物保护学报 2023年第2期50卷 298-305页

作者：高飞谢源潘鑫李洪连张晓婷吴祖建关雄福建农林大学植物保护学院福州350002 河南农业大学植物保护学院省部共建小麦玉米作物学国家重点实验室郑州450002

为监测小麦茎基腐病病原菌的变化,2019—2020年自河南省安阳、濮阳和鹤壁等17个市129个采样点采集小麦茎基腐病病株样品并分离病原菌,对所有分离物进行形态学鉴定,利用特异性引物、ITS和EF-1α基因序列进行分子生物学鉴定,并于室内对优... 详细信息

为监测小麦茎基腐病病原菌的变化,2019—2020年自河南省安阳、濮阳和鹤壁等17个市129个采样点采集小麦茎基腐病病株样品并分离病原菌,对所有分离物进行形态学鉴定,利用特异性引物、ITS和EF-1α基因序列进行分子生物学鉴定,并于室内对优势病原菌菌株进行致病力测定。结果表明,共分离得到892株镰孢菌Fusarium分离物,所得分离物中假禾谷镰孢***共851株,所占比例为95.41%,为优势种;禾谷镰孢***共16株,所占比例为1.79%。除信阳市外,其他16个市优势病原菌均为假禾谷镰孢。不同假禾谷镰孢菌株存在明显的致病力分化,但多数为强致病力菌株。

关键词：小麦茎基腐病病原菌优势种假禾谷镰孢致病力分化

A GNSS Anti-Spoofing Technique Based on the Spatial Distribution Characteristic of the Residual Vectors

在线全文

学校读者我要写书评

暂无评论

Tsinghua Science and Technology 2024年第2期29卷 457-468页

作者： Qi'an wu Xiaowei Cui Mingquan Lu Pengxiang Yang Peng wu Department of Electronic Enginnering Tsinghua UniversityBeijing 100084China Xi’an Modern Control Technology Research Institute Xi’an 710065China

Anti-spoofing is becoming a crucial technique for applications with high navigation accuracy and reliability ***-spoofing technique based on Receiver Autonomous Integrity Monitoring(RAIM)is a good choice for most Global Navigation Satellite System(GNSS)receivers because it does not require any change to the hardware of the ***,the conventional RAIM method can only detect and mitigate a single spoofing *** improved RAIM methods can deal with more spoofing signals,but the computational complexity increases dramatically when the number of satellites in view increase or need additional *** paper proposes a new RAIM method,called the SRV-RAIM method,which has a very low computation complexity regardless of the number of satellites in view and can deal with any number of spoofing *** key to the new method is the spatial distribution characteristic of the Satellites'Residual Vectors(SRV).In replay or generative spoofing scenarios,the pseudorange measurements of spoofing signals are consistent,the residual vectors of real satellites and those of spoofing satellites have good separation characteristics in spatial *** on this characteristic,the SRV-RAIM method is proposed,and the simulation results show that the method can separate the real signals and the spoofing signals with an average probability of 86.55%in the case of 12 visible satellites,regardless of the number of spoofing *** to the conventional traversal-RAIM method,the performance is only reduced by 3.59%,but the computational cost is reduced by 98.3%,so most of the GNSS receivers can run the SRV-RAIM algorithm in time.

关键词： Global Navigation Satellite System(GNSS) anti-spoofing Receiver Autonomous Integrity Monitoring(RAIM) Satellites'Residual Vectors(SRV)