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DNA microarray synthesis by using PDMS molecular stamps (Ⅲ)——Optimization for the reaction conditions

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Chinese Science Bulletin 2002年第13期47卷 1073-1076页

作者： XIAO Pengfeng, LU zuhong, LIU Zhengchun, he Quanguo & he NongyueKey Laboratory for Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096, China Key Laboratory for Molecular and Biomolecular Electronics of Ministry of Education Southeast University Nanjing China

Optimization for the technological processes of fabricating oligonucleotide microarray by the molecular stamping method is studied in this note. Three factors that affect the pressing coupling reactions of the nucleosides are focused on: the stability of the chemical activities of the reaction solutions, the contamination of the remain of the reactive nucleotides among the different spots on the chip, and the influence of the capping reaction on the hybridization result. The experiments show that the acetonitrile solution of tetrazole and nucleoside monomer could maintain sufficient reactive activity for more than 10 h. An effective method has been used and proved to eliminate the residual reactive nucleosides on chip with small molecules containing hydroxylgroup. Finally, the capping step-a regular step in theconventional DNA chemical synthesis can be neglected in our on-chip DNA synthetic process, which would not affect its hybridization results.

关键词： oligonucleotide microarray molecular stamping method DNA chemical synthesis.

Mechanism and application of mesenchymal stem cells and their secreting extracellular vesicles in regulating CD4+T cells in immune diseases

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Biophysics Reports 2024年第6期10卷 403-415页

作者： Zehua Lin Weisong Cai Yuechen Sun Baoai Han Yifan Hu zuhong he Xiong Chen Department of Otolaryngology Head and Neck Surgery Zhongnan Hospital of Wuhan University Sleep Medicine Centre Zhongnan Hospital of Wuhan University

Mesenchymal stem cells（MSCs） show significant promise in treating immune diseases due to their ability to differentiate into various cell types and their immunomodulatory properties. However, the mechanisms by which MSCs regulate CD4+T cells, essential for immune responses, are not yet fully understood. This study aims to provide a comprehensive overview of how MSCs and their secreted extracellular vesicles（EVs） modulate CD4+T cells in immune diseases. We begin by discussing the immunomodulatory properties of MSCs and the factors contributing to their effectiveness. Following this,we explore how MSCs interact with CD4+T cells through various pathways, including the secretion of soluble factors, direct cell-cell contact, and EV-mediated communication. A key focus is on the therapeutic potential of MSC-derived EVs, which are rich in bioactive molecules such as proteins, lipids, and nucleic acids. These molecules can regulate the phenotype and function of CD4+T cells. The challenges and future perspectives in utilizing MSCs and EVs for immune-disease therapy are also ***, this research aims to enhance our understanding of the mechanisms behind MSC-mediated regulation of CD4+T cells and provide insights into the potential use of MSCs and EVs as therapeutic tools in immune diseases. In summary, understanding how MSCs and their EVs control CD4+T cells can offer valuable perspectives for developing innovative immunotherapeutic approaches. Leveraging the immunomodulatory capacity of MSCs and EVs holds promise for managing immune-related disorders.

关键词： Mesenchymal stem cells Exosomes Immune diseases CD4+T cells

Planar distortion quantification to evaluate PDMS stamps affixed on glass support

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Planar distortion quantification to evaluate PDMS stamps aff...

2003年纳米和表面科学与技术全国会议

作者： Quanguo he, Zhengchun Liu, Pengfeng Xiao, Nongyue he, zuhong Lu (Key Laboratory for Molecular and Bio-molecular Electronics of Ministry of Education, Nanjing 210096 China)

This article describes a planar distortion quantification method for PDMS stamps used in soft lithography by introducing an angular parameter θ;the distortion θis proportional to planar distortion in magnitude. We employ this method to evaluate PDMS stamps planar distortions supported on different treated glass with Micron XYZ Scope measurements. The average planar distortion of individual pattern (absolute distortion 0θ1) and their pattern-to-pattern distortion (relative distortion θ2) of PDMS stamps were determined by angular discrepancies (θ). The planar distortion quantification was evaluated among four

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自噬在内耳中的作用及研究进展

中华耳鼻咽喉头颈外科杂志 2020年第11期55卷 1086-1090页

作者：韦新凤贺祖宏邹圣宇柴人杰孔维佳华中科技大学同济医学院附属协和医院耳鼻喉科武汉430022 东南大学生命科学与技术学院南京210096

听觉系统疾病是人类最常见的感觉系统障碍。由于哺乳动物耳蜗毛细胞的不可再生性,其损伤是听力丧失的主要原因。在感音神经性聋的预防及治疗中,维持毛细胞及内耳中其他类型细胞的正常功能是治疗过程中至关重要的环节。自噬是细胞的一种... 详细信息

听觉系统疾病是人类最常见的感觉系统障碍。由于哺乳动物耳蜗毛细胞的不可再生性,其损伤是听力丧失的主要原因。在感音神经性聋的预防及治疗中,维持毛细胞及内耳中其他类型细胞的正常功能是治疗过程中至关重要的环节。自噬是细胞的一种重要的、高度保守的自我调控机制,在正常的生理过程中起"管家"的作用,如清除聚集和错误折叠的蛋白质或受损的细胞器。在应激状态下,自噬则具有维持细胞功能和促存活的作用。本文主要介绍近期关于内耳在不同损伤条件下,如遗传性聋、药物性聋、衰老和噪声暴露所致感音神经性聋中自噬的调控机制及研究进展,阐明自噬有望成为治疗听觉系统疾病新的切入点。

关键词：感音神经性聋听力丧失遗传性聋听觉系统耳蜗毛细胞噪声暴露自噬内耳

一种基于酶切的电化学检测序列特异性DNA结合蛋白的方法

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生命科学仪器 2017年第4期15卷 43-48,63页

作者：潘沁陆祖宏何农跃中国药科大学东南大学生物医学工程学院

我们提出一种新型非标记蛋白质的电化学检测转录因子NF-κB的方法,即将固定在金电极上的通用寡核苷酸通过连接酶形成单分子发夹寡核苷酸,然后进行固相酶促延伸成双链寡核苷酸,最后通过Eco R的酶切信号的变化,采用阳极溶出法和循环伏安... 详细信息

我们提出一种新型非标记蛋白质的电化学检测转录因子NF-κB的方法,即将固定在金电极上的通用寡核苷酸通过连接酶形成单分子发夹寡核苷酸,然后进行固相酶促延伸成双链寡核苷酸,最后通过Eco R的酶切信号的变化,采用阳极溶出法和循环伏安法结合纳米金催化银沉积法检测NF-κB。结果表明本方法具有高特异性、高灵敏度和快速等优点,为单核苷酸突变、药物筛选研究提供有力工具。

关键词：非标记转录因子通用寡核苷酸纳米金颗粒

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老年性聋的发病机制及干预研究进展

中华耳鼻咽喉头颈外科杂志 2020年第11期55卷 1105-1110页

作者：贺祖宏李明邹圣宇丁言言苏红果韦春江韦新凤慕雨容孔维佳华中科技大学同济医学院附属协和医院耳鼻咽喉头颈外科武汉430022

老年性聋是指随着年龄的增加逐渐发生的以高频听力下降为主的感音神经性听力损失,其发病原因及机制复杂,目前缺乏有效的临床治疗手段。本文从发病机制,病理分型,诊断标准及干预措施等方面做一系统综述。

关键词：老年性聋诊断标准感音神经性听力损失系统综述发病机制干预措施病理分型高频听力下降

The applications of nanopores in studies of proteins

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Science Bulletin 2019年第19期64卷 1456-1467页

作者： Taoli Ding Antony KChen zuhong Lu Department of Biomedical Engineering College of EngineeringPeking UniversityBeijing100871China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China

Nanopores are a label-free platform with the ability to detect subtle changes in the activities of individual biomolecules under physiological ***,we comprehensively review the technological development of nanopores,focusing on their applications in studying the physicochemical properties and dynamic conformations of peptides,individual proteins,protein-protein complexes and protein-DNA *** is followed by a brief discussion of the potential challenges that need to be overcome before the technology can be widely accepted by the scientific *** believe that with continued refinement of the technology,significant understanding can be gained to help clarify the role of protein activities in the regulation of cellular physiology and pathogenesis.

关键词： Nanopores Proteins Protein-protein interactions Protein-DNA interactions

Polyp-Canal Reconstruction Reveals Evolution Toward Complexity in Corals

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Research 2024年第1期2023卷 417-428页

作者： Yixin Li Xin Liao Xin Wang Yuanchao Li Hongwei Zhao Yunpeng Zhao Junyuan Chen Chunpeng he zuhong Lu State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China State Key Laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024China Guangxi Key Lab of Mangrove Conservation and Utilization Guangxi Mangrove Research CenterGuangxi Academy of SciencesBeihai 536000China Hainan Academy of Ocean and Fishery Sciences Haikou 571126China State Key Laboratory of Marine Resources Utilization in South China Sea Hainan UniversityHaikou 570228China Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences39 East Beijing RoadNanjing 210008China

Modern scleractinian corals are classified into robust,complex,and basal clades through comparative molecular ***,only few morphological or biological criteria can systematically determine the evolutionary trajectories of these major scleractinian coral ***,we obtained the structural information of 21 scleractinian coral species representing robust and complex clades:High-resolution micro-computed tomography was used to reconstruct the polyp-canal systems in their colonies and to visualize the dynamic polyp growth *** found that the emergence of mesh-like canals may distinguish representatives of complex and robust *** differences in polyp-canal connections suggest distinct evolutionary trajectories among coral species:The formation of the canal network promoted the development of more complex coral structures,and coral polyps within this network formed calices of very similar volume,following precise axial growth *** influence of individual polyps on the coral colony becomes less significant as coral structures become more complex,and coral species with more complicated polyp-canal systems occupied niches more *** work supplements current evolutionary studies on reef-building corals,providing insight for further studies on coral growth patterns.

关键词： Coral representing directions

DNA sequencing by synthesis with degenerate primers

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Journal of Genetics and Genomics 2008年第9期35卷 545-551页

作者： Chao Tang Xiaolong Shi Xiujie Li zuhong Lu State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China The School of Basic Medical Sciences Southeast University Nanfing 210096 China

The degenerate primer-based sequencing was developed by a synthesis method （DP-SBS） for high-throughput DNA sequencing, in which a set of degenerate primers are hybridized on the arrayed DNA templates and extended by DNA polymerase on microarrays. In this method, a different set of degenerate primers containing a given number （n） of degenerate nucleotides at the 3＇-ends were annealed to the sequenced templates that were immobilized on the solid surface. The nucleotides （n＋1） on the template sequences were determined by detecting the incorporation of fluorescent labeled nucleotides. The fluorescent labeled nucleotide was incorporated into the primer in a base-specific manner after the enzymatic primer extension reactions and nine-base length were read out accurately. The main advantage of the DP-SBS is that the method only uses very conventional biochemical reagents and avoids the complicated special chemical reagents for removing the labeled nucleotides and reactivating the primer for further extension. From the present study, it is found that the DP-SBS method is reliable, simple, and cost-effective for laboratory-sequencing a large amount of short DNA fragments.

关键词： microarrays DNA sequencing degenerate primer extension SBS DP-SBS