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The optimization of phenolic compounds extraction from cactus pear(Opuntia ficus-indica) skin in a reflux system using response surface methodology

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Asian Pacific Journal of Tropical Biomedicine 2013年第6期3卷 436-442页

作者： Aguirre Joya Jorge De La Garza Toledo Heliodoro zugasti cruz alejandro Belmares Cerda Ruth Aguilar Cristóbal No Food Research Department School of ChemistryUniversidad Autonóma de Coahuila Basic Science Department Universidad Autonóma Agraria Antonio Narro

Objective:To extract,quantify,and evaluate the phenolic content in Opuntia ficus-indica skin for their antioxidant capacity with three different methods(ABTS,DPPH,and lipid oxidation)and to optimize the extraction conditions[time,temperature and ethanol concentration)in a reflux system.Methods:The extraction process was done using a reflux system.A San CristobalⅡexperimental design with three variables and three levels was used.The variables evaluated were time of extraction(h),concentration of ethanol(%,v/v)and temperature(°C).The extraction process was optimized using a response surface methodology.Results:It was observed that at higher temperature more phenolic compounds were extracted,but the antioxidant capacity was decreased.The optimum conditions for phenolic compounds extraction and antioxidant capacity mixing the three methods were as Follows:45%of ethanol,80℃and 2 hours of extraction.Values obtained in our results are little higher that other previously reported.Conclusions:It can he concluded the by-products of Opuntia ficus-indica represent a good source of natural antioxidants with possible applications in food,cosmetics or drugs industries.

关键词： Opuntia ficus-indica Reflux Phenolics Antioxidants Prickly pear Response surface methodology

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Minimum inhibitory but maximum non-hemolytic concentration of plasma-treated catheters coated with L.tridentata and O.vulgare extracts

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Plasma Science and Technology 2023年第12期25卷 83-96页

作者： Francisco Javier ALONSO-MONTEMAYOR Miriam Desirée DÁVILA-MEDINA alejandro zugasti-cruz Rosa Idalia NARRO-CÉSPEDES María Guadalupe NEIRA-VELÁZQUEZ AidéSÁENZ-GALINDO Eduardo ALONSO-CAMPOS Autonomous University of Coahuila Saltillo 25280Mexico Center for Research in Applied Chemistry Saltillo 25294Mexico University of Monterrey San Pedro Garza García 66238Mexico

Antibacterial extract-coated catheters are promising alternatives to their conventional counterparts,but their hemocompatibility and thermal stability must be studied.Nosocomial bacteria have developed resistance to conventional antibiotics.Herein,the minimum inhibitory but non-hemolytic concentration(MIC-NH)and the thermal stability of Larrea tridentata(L.tridentata)and Origanum vulgare(O.vulgare)extract-coated catheters were studied.Besides,plasma pretreatment was performed to enhance the extract adhesion.Briefly,the extract-coated catheters prevent Staphylococcus aureus colonization without causing hemolysis by using L.tridentata and O.vulgare extracts at MIC-NH(5000 and 2500μg ml~(-1),respectively).Moreover,it has been discovered that the extract coating and plasma treatment improved the thermal stability and the extract adhesion,respectively.Thus,this study provides evidence of alternative antibacterial but non-hemolytic extract-coated catheters.

关键词： antibacterial activity biocompatibility blood-contact device cold plasma nosocomial bacteria

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Mechanosynthesis as a Simple Method to Obtain a Magnetic Composite (Activated Carbon/Fe₃O₄) for Hyperthermia Treatment

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Journal of Biomaterials and Nanobiotechnology 2016年第1期7卷 19-28页

作者： Jorge Carlos Ríos-Hurtado Elia Martha Múzquiz-Ramos alejandro zugasti-cruz Dora Alicia Cortés-Hernández Facultad de Ciencias Químicas Universidad Autónoma de Coahuila Saltillo México Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Saltillo Ramos Arizpe México

A large number of magnetic nanomaterials have been studied for their hyperthermic potential, such as iron oxide based materials. These are embedded in different matrices to improve their properties. In this paper magnetite was synthesized by the coprecipitation method and an activated carbon/magnetite composite was obtained by mechanosynthesis (400 rpm, 3 h). The samples were characterized by X-ray diffraction (XRD), vibrating sample magnetometer (VSM), IR-FT spectroscopy and Scanning Electron Microscopy (SEM). Furthermore, composite heating curves as well as hemolysis tests were performed. The composite showed a superparamagnetic behavior due to its low coercivity index (8.92 Oe) and a high saturation magnetization (40.12 emu/g). SEM images showed that the magnetite was observed on the surface of activated carbon and also the IR-FT spectra indicated that oxygenated groups on the activated carbon surface were responsible for the anchoring of magnetite in the surface, with particle sizes between 9 and 14 nm. Heating results indicated that a composite mass of 18 mg reach a temperature of 45.6°C in a low frequency magnetic field (10.2 kA and 200 kHz). Hemolysis tests indicated that the composite is a non-hemolytic material (4.7% hemolysis). These results demonstrate that the material can be used in magnetic hyperthermia techniques for cancer treatment.

关键词： Mechanosynthesis Hyperthermia Magnetite Activated Carbon Composite

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