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Breakup of 33.4MeV/u ^(17)N on a ^(9)Be Target

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Chinese Physics Letters 2000年第3期17卷 188-190页

作者： HUA Hui JIANG Dong-Xing LI Xiang-Qing QIAN Tao WU He-Yu JIN Gen-Ming ZHAN Wen-Long DUAN Li-Min XIAO Zhi-Guang GUO Zhong-Yan LI zu-Yu WANG hong-Wei WANG Su-Fang Department of Technical Physics Peking UniversityBeijing 100871 Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000

Correlation measurements of neutrons and fragments have been performed in a reaction of 33.4MeV/u ^(17)N on a ^(9)Be *** spectra of n and nitrogen fragments were obtained at different *** on the nuclear diffraction dissociation model,the measured energy spectra and the neutron angular distributions were reproduced quite well assuming that ^(17)N was composed of ^(16)N and n or ^(15)N and 2n interacting with the nonlocal separable *** satisfactory agreement between the theoretical calculations and the experimental data shows that the contribution of Coulomb dissociation is negligible in the reaction concerned.

关键词： potential. dissociation negligible

基于脉冲直流电喷雾离子化的自动化原位质谱分析系统及其应用

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分析化学 2023年第3期51卷 348-355页

作者：施露露洪欢欢陈安琪唐燕张涛闻路红赵鹏中国科学院上海技术物理研究所宁波华仪宁创智能科技有限公司宁波大学高等技术研究院

脉冲直流电喷雾离子化质谱（PDESI-MS）分析具有原位、实时、快速和可分析微量样品等优点。然而，目前的PDESI-MS系统需手动操作进行样品处理和质谱进样，存在操作繁琐、步骤多、误差大和准确度低等问题。本工作研制了一套高通量、全自... 详细信息

脉冲直流电喷雾离子化质谱（PDESI-MS）分析具有原位、实时、快速和可分析微量样品等优点。然而，目前的PDESI-MS系统需手动操作进行样品处理和质谱进样，存在操作繁琐、步骤多、误差大和准确度低等问题。本工作研制了一套高通量、全自动化和一体化的PDESI-MS系统，探索了其在真核生物（单个膀胱癌细胞）和原核生物（细菌单菌落）中小分子代谢物原位检测分析中的应用。结果表明，本自动化PDESI-MS系统可有效检测不同菌种代谢物，实现了不同类型大肠杆菌的分类定型；同时，皮升级液滴产生技术使其可应用于单细胞检测。自动化PDESI-MS系统可实现高精度、高密度、高重复度和高自动化的原位样品萃取，特别是微量小体积样品的分析，在食品安全、再生医学、药物筛选和临床治疗等领域具有极大的应用潜力和良好的发展前景。

关键词：脉冲直流电喷雾离子化自动化原位检测微液滴

Polyoxygenated Cembranoids from Soft Coral Lobophytum Crassum and Their Anti-tumoral Activities

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Chinese Journal of Chemistry 2021年第3期39卷 640-646页

作者： Fang-Zhou Yin Xia-Juan Huan Wayan Mudianta Ze-hong Miao hong Wang Yue-Wei Guo Xu-Wen Li State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of Sciences555 Zu Chong Zhi RoadZhangjiang Hi-Tech ParkShanghai 201203China Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals and College of Pharmaceutical Science Zhejiang University of TechnologyHangzhouZhejiang 310014China Department of Chemistry Universitas Pendidikan GaneshaBali 81116Indonesia

Main observation and conclusion Four new cembranoids,6-oxo-cembrene-A(1),lobocrassins G-H(2-3),and 14-epi-lobophytolide B(4),along with eight known re-lated compounds(5-12),were isolated from the South China Sea soft coral Lobophytum *** structures were established by extensive spectroscopic analysis and by comparison of the spectral data with those reported in the *** absolute stere-ochemistry of 14-epi-lobophytolide B(4)was established by time-dependent density functional theory electronic circular dichroism(TDDFT-ECD)calculations,whereas that of three known metabolites(6,11 and 12)was also determined,for the first time,by using modified Mosher's method and/or X-ray diffraction *** bioassay,the cembranoids 6,7 and 12 showed significant an-ti-tumoral activity against A549,HT-29,SNU-398 and Capan-1 tumor cell lines with IC_(50) values ranging from 1.5 to 7.4μmol/L.A pre-liminary structure-activity relationship was also discussed.

关键词： Soft coral Lobophytum crassum Cembranoids Anti-tumoral activity Stereochemistry

基于患者量表数据比较单髁置换术与人工全膝关节置换术的临床疗效

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中国修复重建外科杂志 2021年第12期35卷 1574-1579页

作者：祖翔宇王俊陆鸣尹宗生安徽医科大学第一附属医院关节外科合肥230022

目的比较单髁置换术(unicompartmental knee arthroplasty,UKA)和人工全膝关节置换术(total knee arthroplasty,TKA)患者术后膝关节功能、关节遗忘度和生活质量。方法回顾分析2017年9月—2018年6月接受UKA或TKA治疗且符合选择标准的患... 详细信息

目的比较单髁置换术(unicompartmental knee arthroplasty,UKA)和人工全膝关节置换术(total knee arthroplasty,TKA)患者术后膝关节功能、关节遗忘度和生活质量。方法回顾分析2017年9月—2018年6月接受UKA或TKA治疗且符合选择标准的患者临床资料,经倾向评分匹配后最终纳入研究的TKA组和UKA组患者均为40例(40膝)。两组患者性别、年龄、身体质量指数、手术侧别及术前膝关节活动度、西安大略和麦克马斯特大学骨关节炎指数(WOMAC)评分、膝关节学会评分(KSS)临床和功能评分比较差异均无统计学意义(P>0.05)。术后2年对两组患者行WOMAC评分、KSS临床和功能评分,并与术前比较;同时行膝关节损伤和骨关节炎结局评分-身体功能(KOOS-PS)、简明健康调查量表(SF-36量表)及关节遗忘评分(FJS)。结果术后2年,两组患者WOMAC总分及KSS临床和功能评分均较术前显著改善(P0.05)。UKA组KOOS-PS总分明显低于TKA组(t=4.243,P=0.000),其中UKA组在扭动/以膝盖为中心转动、跪下、蹲着3项评分上显著低于TKA组(P<0.05)。UKA组FJS总分明显高于TKA组(t=−6.334,P=0.000),其中UKA组在当步行超过15 min、当爬楼梯时、当不在平坦的地面行走时、当长期站立时、当做家务或园艺时、当散步或远足时、当做最喜欢的运动时7项评分上显著低于TKA组(P<0.05)。UKA组SF-36量表评分中的生理机能、精力、社会职能、情感职能和精神健康5项评分显著高于TKA组(P<0.05)。结论与TKA相比,UKA患者的膝关节功能可能恢复更好,关节遗忘度和生活质量可能更高。

关键词：单髁置换术人工全膝关节置换术膝关节功能关节遗忘度生活质量

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应用光谱技术快速测定发动机润滑油的粘度值

光谱学与光谱分析 2010年第9期30卷 2496-2499页

作者：赵芸蒋璐璐张瑜谈黎虹何勇浙江大学生物系统工程与食品科学学院浙江杭州310029 浙江科技学院信息与电子工程学院浙江杭州310023 浙江经济职业技术学院汽车技术学院浙江杭州310018

提出了一种应用可见近红外光谱技术快速测定发动机润滑油动力粘度值的新方法。对5种不同粘度的润滑油共150个样本进行光谱扫描,经过光谱预处理后应用偏最小二乘法(PLS)建立了润滑油动力粘度值的预测模型,并提取出前6个有效主成分作为最... 详细信息

提出了一种应用可见近红外光谱技术快速测定发动机润滑油动力粘度值的新方法。对5种不同粘度的润滑油共150个样本进行光谱扫描,经过光谱预处理后应用偏最小二乘法(PLS)建立了润滑油动力粘度值的预测模型,并提取出前6个有效主成分作为最小二乘-支持向量机(LS-SVM)预测模型的输入变量,建立相应的最小二乘-支持向量机(LS-SVM)预测模型,采用径向基函数(RBF)作为核函数,超参数γ和RBF核函数参数σ2的最佳组合为γ=27.321 2和σ2=3.229 5。用125个样本建模,25个样本验证。实验结果表明,LS-SVM模型比PLS模型能获得更满意的预测效果。说明应用光谱技术可以实现发动机润滑油动力粘度值的快速无损检测。

关键词：润滑油动力粘度可见/近红外光谱偏最小二乘法支持向量机

Corynoxine B targets at HMGB1/2 to enhance autophagy forα-synuclein clearance in fly and rodent models of Parkinson's disease

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药学学报:英文版 2023年第6期13卷 2701-2714页

作者： Qi Zhu Juxian Song Jia-Yue Chen Zhenwei Yuan Liangfeng Liu Li-Ming Xie Qiwen Liao Richard D.Ye Xiu Chen Yepiao Yan Jieqiong Tan Chris Soon Heng Tan Min Li Jia-hong lu State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao 999078China Medical College of Acupuncture-Moxibustion and Rehabilitation Guangzhou University of Chinese MedicineGuangzhou 510006China Mr.and Mrs.Ko Chi Ming Centre for Parkinson's Disease Research School of Chinese MedicineHong Kong Baptist UniversityHongKong 999077China Guangdong Corporate Key Laboratory of High-end Liquid Medicine R&D IndustrializationShaoguan 512028China Limin Pharmaceutical Factory Livzon Pharmaceutical Group Inc.Shaoguan 512028China Kobilka Institute of Innovative Drug Discovery School of Medicinethe Chinese University of Hong KongShenzhen 518172China Center for Medical Genetics Life Science SchoolCentral South UniversityChangsha 410031China Department of Chemistry College of ScienceSouthern University of Science and TechnologyShenzhen 518055China

Parkinson's disease(PD)is the most common neurodegenerative movement *** is featured by abnormal alphα-synuclein(α-syn)aggregation in dopaminergic neurons in the substantia ***(autophagy)is an evolutionarily conserved cellular process for degradation of cellular contents,including protein aggregates,to maintain cellular *** B(Cory B),a natural alkaloid isolated from Uncaria rhynchophylla(Miq.)Jacks.,has been reported to promote the clearance ofα-syn in cell models by inducing ***,the molecular mechanism by which Cory B induces autophagy is not known,and theα-syn-lowering activity of Cory B has not been verified in animal ***,we report that Cory B enhanced the activity of Beclin 1/VPS34 complex and increased autophagy by promoting the interaction between Beclin 1 and HMGB1/*** of HMGB1/2 impaired Cory B-induced *** showed for the first time that,similar to HMGB1,HMGB2 is also required for autophagy and depletion of HMGB2 decreased autophagy levels and phosphatidylinositol 3-kinaseⅢactivity both under basal and stimulated *** applying cellular thermal shift assay,surface plasmon resonance,and molecular docking,we confirmed that Cory B directly binds to HMGB1/2 near the C106 ***,in vivo studies with a wild-typeα-syn transgenic drosophila model of PD and an A53Tα-syn transgenic mouse model of PD,Cory B enhanced autophagy,promotedα-syn clearance and improved behavioral *** together,the results of this study reveal that Cory B enhances phosphatidylinositol 3-kinaseⅢactivity/autophagy by binding to HMGB1/2 and that this enhancement is neuroprotective against PD.

关键词： Corynoxine B Parkinson's disease Neurodegenerative disease α-Synuclein Autophagy PI3KC3 HMGB1 HMGB2

Cross-neutralizing Anti-hemagglutinin Antibodies Isolated from Patients Infected with Avian Influenza A(H5N1) Virus

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Biomedical and Environmental Sciences 2020年第2期33卷 103-113页

作者： SUN Ying CAO Yang LI Zi BAI Tian ZHANG hong HU Shi Xiong LI Fang Cai ZHAO Xiang CHEN Yong Kun lu Jian LIU Li Qi WANG Da Yan SHU Yue Long ZHOU Jian Fang National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionKey Laboratory for Medical VirologyNational Health and Family Planning CommissionBeijing 102206China Center of Growth Metabolism and AgingKey Laboratory of Bio-Resource and Eco-EnvironmentMinistry of EducationCollege of Life SciencesSichuan UniversityChengdu 610064SichuanChina Hunan Provincial Disease Control and Prevention Changsha 410000HunanChina School of Public Health(Shenzhen) Sun Yatsen UniversityShenzhen 510275GuangdongChina

Objective To recover broad-neutralizing monoclonal antibodies(Bn Abs)from avian influenza A(H5N1)virus infection cases and investigate their genetic and functional *** We screened the Abs repertoires of expanded B cells circulating in the peripheral blood of H5N1 *** genetic basis,biological functions,and epitopes of the obtained Bn Abs were assessed and *** Two Bn Abs,2-12 D5,and 3-37 G7.1,were respectively obtained from two human H5N1 cases on days 12 and 21 after disease *** Abs demonstrated cross-neutralizing and Ab-dependent cellular cytotoxicity(ADCC)*** derived from distinct Ab lineages,i.e.,V^H1-69-D2-15-JH^4(2-12D5)and V^H1-2-D3-9-JH^5(3-32 G7.1),the Bn Abs were directed toward CR6261-like epitopes in the HA stem,and HA2 I45 in the hydrophobic pocket was the critical residue for their *** motifs for binding with the HA stem,namely,IFY in VH1-69-encoded Abs and LXYFXW in D3-9-encoded Abs,were also observed in 2-12D5 and 3-32 G7.1,*** Cross-reactive B cells of different germline origins could be activated and re-circulated by avian influenza *** HA stem epitopes targeted by the Bn Abs,and the two Ab-encoding genes usage implied the VH1-69 and D3-9 are the ideal candidates triggered by influenza virus for vaccine development.

关键词： V^H1-69 D3-9 Avian influenza A(H5N1)virus Cross-neutralizing Antibody

高速逆流色谱法分离制备母丁香和公丁香中的5种非挥发性化合物

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色谱 2011年第11期29卷 1112-1117页

作者：高璐于波杨红辽宁省生物技术与分子药物研发重点实验室辽宁师范大学生命科学学院辽宁大连116029

应用高速逆流色谱法从母丁香和公丁香中快速分离了3种已知非挥发性化合物,并利用相同方法从公丁香中分离出2种色原酮类化合物。两相溶剂系统A为正己烷-乙酸乙酯-甲醇-水(5∶8∶6∶13,v/v/v/v),系统B为正己烷-乙酸乙酯-甲醇-水(5∶8∶9∶... 详细信息

应用高速逆流色谱法从母丁香和公丁香中快速分离了3种已知非挥发性化合物,并利用相同方法从公丁香中分离出2种色原酮类化合物。两相溶剂系统A为正己烷-乙酸乙酯-甲醇-水(5∶8∶6∶13,v/v/v/v),系统B为正己烷-乙酸乙酯-甲醇-水(5∶8∶9∶10,v/v/v/v),以系统A的上相为固定相,系统A和B的下相为流动相,利用梯度洗脱方式,在主机转速为880 r/min、流速1.2 mL/min条件下,成功地从70 mg母丁香粗提物中分离得到12.3 mg鞣花酸、9.6 mg鼠李素、17.2 mg槲皮素,从50 mg公丁香粗提物中分离得到5,7-二甲氧基-2-甲基色原酮10.2mg、5,7-二甲氧基-2,6-二甲基色原酮8.6 mg,纯度均在96%以上。各化合物的结构均由质谱和核磁共振氢谱、碳谱鉴定。利用该方法可以对丁香不同药用部位中的非挥发性化合物进行有效的分离和纯化。

关键词：高速逆流色谱非挥发性化合物母丁香公丁香制备

Analysis of the function of D279N mutation of VP2 of infectious bursal disease virus

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Journal of Integrative Agriculture 2015年第12期14卷 2618-2625页

作者： QI Xiao-le lu Zhen WANG Nian CHEN Yu-ming ZHANG Li-zhou GAO Li LI Kai REN Xian-gang WANG Yong-qiang GAO hong-lei GAO Yu-long Nicolas Eterradossi WANG Xiao-mei Division of Avian Infectious Diseases State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Avian and Rabbit Virology Immunology and Parasitology Unit OIE Reference Laboratory for Infectious Bursal Disease French Agency for Food Environmental and Occupational Health Safety (Anses) Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses

Infectious bursal disease virus（IBDV）is responsible for the highly contagious infectious bursal disease of *** understanding the gene-function is necessary to design the tailored *** amino acid residue 279,located on strand P_F of VP2,is one of the three residues that have been reported to be involved in cell-tropism but with some *** this study,to further clarify the amino acids involved in the cell tropism of IBDV,a series of mutations about residue 279were introduced into the VP2 of vv IBDV Gx *** the reverse genetic system,we found single mutation of D279N,double mutations of D279N/A284T or Q253H/D279N were not enough to adapt IBDV to chicken embryo fibroblast（CEF）*** evaluate whether residue 279 could influence the replication and virulence of IBDV,the virus r Gx HT-279 with three mutations（Q253H/D279N/A284T）was rescued and *** showed that the mutation of residue 279 in VP2had no efficient effects on both the replication efficiency in vitro and the virulence to SPF chickens of *** summary,the results demonstrated that residue 279 of VP2 did not contribute efficiently to cell tropism,replication efficiency,and virulence of IBDV at least in some *** findings provided further information for understanding the gene function of IBDV.

关键词： infectious bursal disease virus(IBDV) residue 279 cell tropism virulence