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Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy

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International Journal of Ophthalmology(English edition) 2020年第6期13卷 927-934页

作者： ANDrea Krisztina Sükosd Krisztina Szabadfi Edina Szabó-Meleg Beáta Gáspár Pavel Stodulka Gyorgy SétálóJr Róbert Gábriel Miklós Nyitrai zsolt Biró Hajnalka Abrahám Department of Ophthalmology University of Pecs Medical School and Clinical CentrePecs 7623Hungary Department of Experimental Zoology and Neurobiology University of PecsPecs 7624Hungary Department of Biophysics University of Pecs Medical School and Clinical CentrePecs 7624Hungary Janos Szentagothai Research Centre University of PecsPecs 7624Hungary Optimum Laser Centre Budapest 1124Hungary Gemini Eye Clinics Zlin 76001Czech Republic Department of Medical Biology and Central Electron Microscopic Laboratory University of Pecs Medical SchoolPecs 7624Hungary

AIM:To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery(CPS)and femtosecond laserassisted cataract surgery(FLACS).METHODS:The cytoskeleton of the epithelial cells of the anterior lens capsules(ALC)removed by CPS and FLACS was examined by *** of the intermediate filament,glial fibrillary acidic protein(GFAP),and glutamine synthetase(GS)immunoreactivity were *** order to map the actin network of cells,fluorescently labeled phalloidin was *** samples were examined using confocal laser scanning ***:GFAP expression was visible in a larger number of the epithelial cells after CPS compared to *** CPS sample’s epithelial cells,GFAP immunoreactivity indicated robust morphological *** the actin filaments,the presence of tubular elements connecting epithelial cells,regular actin pattern and marked cortical network after CPS were *** FLACS,the actin cytoskeleton of the epithelial cells remained densely structured,and the tubular elements were undetectable,however,the above-mentioned regular actin pattern and the marked cortical network were ***:The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells.

关键词： lens epithelial cell cytoskeleton capsulotomy glial fibrillary acidic protein actin

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欧元区经济增长的挑战及其政策选择

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新金融评论 2014年第4期 104-108页

作者： zsolt Darvas Bruegel

总体而言,我认为对欧盟区的评论是不正确的,一方面,欧洲经济萧条比我们料想的更严重;另一方面,在欧盟区经常账户还存在赤字,危机之前,其他地区的金融一体化也非常脆弱。我们现在进行结构性改革,不可能一蹴而就,欧元区各个国家的情况也... 详细信息

总体而言,我认为对欧盟区的评论是不正确的,一方面,欧洲经济萧条比我们料想的更严重;另一方面,在欧盟区经常账户还存在赤字,危机之前,其他地区的金融一体化也非常脆弱。我们现在进行结构性改革,不可能一蹴而就,欧元区各个国家的情况也不尽相同。欧洲人民对民主的满意度出现了严重的分化,并由此造成了负面的影响。相应的政策选择包括:一是南欧国家应该调整财政政策,二是建立欧元区的良好环境,三是应该更好地使用欧盟的合作体制。

关键词：萧条经常账户赤字结构性改革民主满意度

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整体解决方案对转速调节控制的冲击

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现代制造 2016年第34期 32-34页

作者： zsolt Pekker

在许多生产企业中，产生真空所消耗的能源是能量平衡的重要项目之一。因此，产生真空时的效率高低显得越来越重要，但迄今为止真空泵节能运行的关键技术还没有。

关键词：整体解决方案调节控制转速生产企业能量平衡节能运行真空泵

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酸面团优选组合菌对苦荞麸皮馒头品质的影响

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食品与机械 2016年第12期32卷 201-205,223页

作者：王雪婷杜木英廖钰婷何瑞李玉珠 zsolt Zalán 西南大学食品科学学院重庆400715 西南大学重庆市特色食品工程技术研究中心重庆400715 匈牙利国家农业研究和创新中心食品科学研究所匈牙利布达佩斯999024

以安琪酵母和匈牙利酵母发酵苦荞麸皮馒头为对照,通过感官评价和质构分析,同时采用固相微萃取—气相色谱—质谱联用技术,研究酸面团优选组合菌对苦荞麸皮馒头的品质影响。结果表明:3种发酵剂馒头感官评分为:组合菌种(82.2±2.3)>匈牙利... 详细信息

以安琪酵母和匈牙利酵母发酵苦荞麸皮馒头为对照,通过感官评价和质构分析,同时采用固相微萃取—气相色谱—质谱联用技术,研究酸面团优选组合菌对苦荞麸皮馒头的品质影响。结果表明:3种发酵剂馒头感官评分为:组合菌种(82.2±2.3)>匈牙利酵母(80.9±1.3)>安琪酵母(77.1±1.4);在硬度、咀嚼性、胶粘性、内聚性和回复力方面,组合菌组最佳;在弹性方面,匈牙利酵母组最佳,总体上组合菌组的质构特性更佳;在风味物质上,安琪酵母组共检测到44种风味成分,匈牙利酵母组共检测到49种,组合菌组共检测到52种,并且组合菌组的醛类、呋喃类、酯类、酚类以及同类都比安琪酵母组和匈牙利酵母组的相对含量高,还检测到3种特有的酯类物质,说明组合菌种发酵更利于苦荞麸皮馒头风味物质的形成;表明组合菌更有利于发酵苦荞麸皮馒头,更易让消费者接受,为组合菌种的应用提供理论支持。

关键词：苦荞麸皮馒头组合菌风味物质

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Systematic review of the old and new concepts in the epithelial-mesenchymal transition of colorectal cancer

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World Journal of Gastroenterology 2016年第30期22卷 6764-6775页

作者： Simona Gurzu Camelia Silveanu Annamaria Fetyko Vlad Butiurca zsolt Kovacs Ioan Jung Department of Pathology University of Medicine and Pharmacy of Tirgu-Mures Department of Surgery University of Medicine and Pharmacy of Tirgu-Mures Departments of Genetics and Biochemistry University of Medicine and Pharmacy of Tirgu-Mures

Epithelial-to-mesenchymal transition(EMT) is defined as the transformation of an epithelial cell into a spindle cell with the loss of membrane E-cadherin expression and the gain of mesenchymal markers positivity. In the field of colorectal cancer(CRC), first data about EMT was published in 1995 and more than 400 papers had been written up to March 2016. Most of them are focused on the molecular pathways and experimentally-proved chemoresistance. In the present article, an update in the field of EMT in CRC based on the review of the literature and personal experience of the authors is presented. The information about the molecular and immunohistochemical(IHC) particularities of these processes and their possible role in the prognosis of CRC were also up-dated. This article focuses on the IHC quantification of the EMT, the immunoprofile of tumor buds and on the relation between EMT, angiogenesis, and stem cells activation. The EMT-induced chemoresistance vs chemotherapyor radiotherapy-induced EMT and cellular senescence was also synthesized for both conventional and targeted therapy. As a future perspective, the EMTangiogenesis-stemness link could be used as a possible valuable parameter for clinical follow-up and targeted therapeutic oncologic management of patients with CRC. Association of dexamethasone and angiotensin converting enzyme inhibitors combined with conventional chemotherapies could have clinical benefits in patients with CRC. The main conclusion is that, although many studies have been published, the EMT features are still incompletely elucidated and newly discovered EMT markers provide confusing data in understanding this complicated process, which might have significant clinical impact.

关键词： Angiogenesis Colorectal cancer Budding Epithelial-mesenchymal transition Chemoresistance

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Chronic kidney disease severely deteriorates the outcome of gastrointestinal bleeding: A meta-analysis

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World Journal of Gastroenterology 2017年第47期23卷 8415-8425页

作者： Roland Hágendorn Nelli Farkas Aron Vincze Zoltán Gyongyi Dezso Csupor Judit Bajor Bálint Eross Péter Csécsei Andrea Vasas zsolt Szakács László Szapáry Péter Hegyi Alexandra Mikó Department of Gastroenterology First Department of MedicineUniversity of Pécs Institute of Bioanalysis University of Pécs Department of Public Health Medicine University of Pécs Department of Pharmacognosy Faculty of PharmacyUniversity of Szeged Institute for Translational Medicine University of Pécs Department of Neurology University of Pécs

AIM To understand the influence of chronic kidney disease(CKD) on mortality, need for transfusion and rebleeding in gastrointestinal(GI) bleeding *** A systematic search was conducted in three databases for studies on GI bleeding patients with CKD or endstage renal disease(ESRD) with data on outcomes of mortality, transfusion requirement, rebleeding rate and length of hospitalization(LOH). Calculations were performed with Comprehensive Meta-Analysis software using the random effects model. Heterogeneity was tested by using Cochrane's Q and I2 statistics. Mean difference(MD) and OR(odds ratio) were ***1063 articles(EMBASE: 589; PubM ed: 459; Cochrane: 15) were found in total. 5 retrospective articles and 1 prospective study were available for analysis. These 6 articles contained data on 406035 patients, of whom 51315 had impaired renal function. The analysis showed a higher mortality in the CKD group(OR = 1.786, 95%CI: 1.689-1.888, P < 0.001) and the ESRD group(OR = 2.530, 95%CI: 1.386-4.616, P = 0.002), and a rebleeding rate(OR = 2.510, 95%CI: 1.521-4.144, P < 0.001) in patients with impaired renal function. CKD patients required more unit red blood cell transfusion(MD = 1.863, 95%CI: 0.812-2.915, P < 0.001) and spent more time in hospital(MD = 13.245, 95%CI: 6.886-19.623, P < 0.001) than the *** ESRD increases mortality, need for transfusion, rebleeding rate and LOH among GI bleeding patients. Prospective patient registries and observational clinical trials are crucially needed.

关键词： Gastrointestinal bleeding Chronic kidney disease Mortality Blood transfusion Rebleeding

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World Journal of Gastroenterology 2016年第47期22卷 10325-10340页

作者： Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres zsolt Tulassay Béla Molnár Molecular Medicine Research Group Hungarian Academy of Sciences 2~(nd) Department of Internal Medicine Semmelweis University 1~(st) Department of Paediatrics Semmelweis University

AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related "epigenetic clock" genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture *** Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased S

关键词： DNA methylation Aging Colorectal cancer Adenoma Epigenetic drift Epigenetic clock Secreted frizzled related protein 1

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NKX2-3 and IRGM variants are associated with diseasesu sceptibility to IBD in Eastern European patients

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World Journal of Gastroenterology 2010年第41期16卷 5233-5240页

作者： Nora Meggyesi Lajos S Kiss Magdalena Koszarska Martin Bortlik Dana Duricova Laszlo Lakatos Tamas Molnar Martin Lenicek Libor Vítek Istvan Altorjay Maria Papp zsolt Tulassay Pal Miheller Janos Papp Attila Tordai Hajnalka Andrikovics Milan Lukas Peter Laszlo Lakatos Department of Molecular Diagnostics Hun-garian National Blood Transfusion Service H-1113 BudapestHungary 1st Depart-ment of Medicine Semmelweis University H-1083 BudapestHungary IBD Clinicaland Research Centre ISCARE IVF and 1st Medical FacultyCharles University 170 04 Prague Czech Republic 1st Department of Medicine Csolnoky F.County Hospital H8200 Veszprem Hungary 1st Department of Medicine University ofSzeged H6720 Szeged Hungary Department of Biochemistry andLaboratory Diagnostics 1st Medical Faculty Charles Univer-sity 170 04 Prague Czech Republic 2nd Department of Medicine University of Debrecen H4032 Debrecen Hungary 2nd Department of Medicine Semmelweis University H1085 Budapest Hungary

AIM: To investigate variants of immunity-related GT-Pase family M (IRGM) and NKX2-3 genes and genotype-phenotype in Eastern European patients with inflammatory bowel disease (IBD).METHODS: We analyzed 1707 Hungarian and Czech subjects with Crohn’s disease (CD) (n = 810, age: 37.1 ± 12.6 years, duration: 10.7 ± 8.4 years) and ulcerative colitis (UC) (n = 428, age: 43.7 ± 15.0 years, duration: 12.6 ± 9.9 years), as well as 469 healthy controls. IRGM rs13361189, NKX2-3 rs10883365 and ECM1 rs13294 polymorphisms were tested by LightCy-cler allele discrimination. Detailed clinical phenotypes were determined by reviewing the medical charts. RESULTS: NKX2-3 rs10883365 variant allele was as-sociated with increased risk for CD (P = 0.009, OR = 1.24, 95% CI = 1.06-1.48) and UC (P = 0.001, OR = 1.36, 95% CI = 1.13-1.63), whereas variant IRGM allele increased risk for CD (P = 0.029, OR = 1.36, 95% CI = 1.03-1.79). In contrast, ECM1 rs13294 was not associat-ed with either CD or UC. In CD, the variant IRGM allele was associated with a colon-only location (P = 0.02, OR = 1.62, 95% CI = 1.07-2.44), whereas in UC, the ECM1 variant was associated with cutaneous manifestations (P = 0.002, OR = 3.36, 95% CI = 1.48-7.63). Variant alleles did not predict resistance to steroids or azathio-prine, efficacy of infliximab, or need for surgery. CONCLUSION: NKX2-3 and IRGM are susceptibility locifor IBD in Eastern European patients. Further studies are needed to confirm the reported phenotype-genotype associations.

关键词： Crohn’s disease Ulcerative colitis NKX2-3 Immunity-related GTPase family M ECM1 Genotype Phenotype Pharmacogenetics

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荧光假单胞菌ZX对葡萄采后灰霉病的防治

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食品工业科技 2021年第22期42卷 125-132页

作者：魏雪江孟遥钟涛张曼王智荣 zsolt Zalan FERENC Hegyi KRISZTINA Takacs 杜木英西南大学食品科学学院重庆400715 中匈食品科学联合研究中心重庆400715 匈牙利国家农业创新研究中心匈牙利布达佩斯1022 农业部农产品贮藏保鲜质量安全风险评估实验室(重庆) 重庆400715

研究荧光假单胞菌(Pseudomonas fluorescens)ZX对葡萄采后灰霉病的防治效果。以“巨峰”葡萄为试材,测定*** ZX的控病效果和生长动态,在离体条件下通过孢子萌发观察、挥发性物质抑菌测试、扫描电镜探究其对灰葡萄孢霉(Botrytis cinerea... 详细信息

研究荧光假单胞菌(Pseudomonas fluorescens)ZX对葡萄采后灰霉病的防治效果。以“巨峰”葡萄为试材,测定*** ZX的控病效果和生长动态,在离体条件下通过孢子萌发观察、挥发性物质抑菌测试、扫描电镜探究其对灰葡萄孢霉(Botrytis cinerea)的防治。结果表明,使用*** ZX处理,葡萄果实的发病率仅为24.8%,明显低于对照组(发病率64.67%),病斑直径最小,可以明显抑制***孢子萌发、芽管伸长。*** ZX产生的挥发性物质具有较强的抑菌效果,并且能对***有重寄生作用,破坏菌丝的正常形态。另外,无论是浸泡还是接种处理,*** ZX都能在葡萄果实上快速地定殖生长。由此表明,*** ZX通过抑制***生长来控制葡萄采后灰霉病,为果实采后防腐保鲜提供一种新的选择。

关键词：荧光假单胞菌生物防治葡萄采后病害

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DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods,using four commercially kits

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World Journal of Clinical Oncology 2019年第9期10卷 307-317页

作者： zsolt Kovacs Ioan Jung Erzsebet Csernak Zoltan Szentirmay Laura Banias Genoveva Rigmanyi Simona Gurzu Department of Pathology University of MedicinePharmacySciences and TechnologyTargu-Mures 530149Romania Research Center(CCAMF) University of MedicinePharmacySciences and TechnologyTargu-Mures 540139Romania

BACKGROUND Nucleic acid isolation from formalin-fixed, paraffin-embedded tissue(FFPET)samples is a daily routine in molecular pathology laboratories, but extraction from FFPET is not always easily achieved. Choosing the right extraction technique is key for further *** To compare the performance of four commercially available kits used for DNA extraction in routine *** DNA isolation was performed on 46 randomly selected formalin-fixed, paraffinembedded(FFPE) colorectal adenocarcinoma(CRC) surgical specimens. Four commercially available extraction kits were used: two for manual DNA extraction(the Pure Link Genomic DNA Mini Kit from Invitrogen and the High Pure FFPE DNA Isolation Kit from Roche) and two for automated DNA extraction(the i Prep Genomic DNA Kit from Invitrogen and the Magna Pure LC DNA Isolation Kit from Roche). The DNA concentration and quality(odds ratio) among the four systems were compared. The results were correlated with the clinicopathological aspects of CRC cases: age, gender, localization, macro-and microscopic features,lymph node metastases, and the lymph node *** The highest DNA concentration was obtained using the manual kits: 157.24 ±62.99 ng/μL for the Pure Link Genomic DNA Mini Kit and 86.64 ng/μL± 43.84 for the High Pure FFPE DNA Isolation Kit(P < 0.0001). Lower concentrations were obtained with automated systems: 20.39 ± 21.19 ng/μL for the Magna Pure LC DNA Isolation Kit and 8.722 ± 6.408 ng/μL for the i Prep Genomic DNA Kit,with differences between the systems used(P < 0.0001). The comparison between age, gender, tumor localization, pT or pN stage and the lymph node ratio indicated no statistically significant difference in DNA concentration using any of the nucleic acid isolation kits. DNA concentration was influenced by the macroscopic features and grade of differentiation. A higher DNA concentration was obtained for well-differentiated polypoid colorectal adenocarcinomas(CRCs), compared with undif

关键词： DNA isolation Colorectal cancer Paraffin-embedded PureLink Genomic DNA Mini Kit High Pure FFPE DNA Isolation Kit iPrep Genomic DNA Kit MagnaPure LC DNA Isolation Kit

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