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IN SITU IMAGING OF BREAST CANCER CELLS USING GREEN SEMICONDUCTOR QUANTUM DOTS

Transactions of Nanjing University of Aeronautics and Astronautics 2010年第1期27卷 13-20页

作者：许恒毅 zoraida p. aguilar 苏怀朋 Benjamin J. Jones John. D. Dixon 熊勇华魏华 Andrew Y. Wang 南昌大学食品科学与技术国家重点实验室 Ocean NanoTech LLC 2143 Worth LaneSpringdaleusa72764

The breast cancer is the most common cause of cancer death in women. To establish an early stage in situ imaging of breast cancer cells, green quantum dots （QDs） are used as a fluorescent signal generator. The QDs based imaging of breast cancer cells involves anti-HER2/neu antibody for labeling the over exp.essed HER2 on the surface of breast cancer cells. The comp.ete assay involves breast cancer cells, biotin labeled antibody and strep.avidin conjugated QDs. The breast cancer cells are grown in culture p.ates and exp.sed to the biotin labeled antibodies, and then exp.sed to strep.avidin labeled QDs to utilize the strong and stable biotin-strep.avidin interaction. Fluorescent images of the comp.ete assay for breast cancer cells are evaluated on a microscop. with a UV light source. Results show that the breast cancer cells in the comp.ete assay are used as fluorescent cells with brighter signals comp.red with those labeled by the organic dye using similar p.rameters and the same number of cells.

关键词： in situ p.ocessing quantum op.ics breast cancer cells non-sp.cific binding immuno-histochemical imaging

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In vitro cytotoxicity of CdSe/ZnS quantum dots with different surface coatings to human keratinocytes HaCaT cells

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Journal of Environmental Sciences 2013年第1期25卷 163-171页

作者： Kavitha p.thakoti Huey-Min Hwang Hong Xu zoraida p.aguilar Andrew Wang Dep.rtment of Biology Jackson State University Ocean NanoTech 2143 Worth LaneSpringdale Arkansas 72764KUSA

Quantum dots （QD） nanop.rticles have been widely used in biomedical and electronics fields, because of their novel op.ical p.op.rties. Consequently it confers enormous p.tential for human exp.sure and environmental release. To increase the biocomp.tibility of QDs, a variety of surface coatings or functional group. are added to increase their bioactivity and water solubility. Human adult low calcium high temp.rature （HaCaT） cells are the ep.thelial cells derived from adult human skin that exhibits normal differentiation cap.city and a DNA fingerp.int p.ttern that is unaffected by long-term cultivation, transformation, or the p.esence of multip.e chromosomal alternations. Human keratinocytes, HaCaT cells were used to systematically evaluate the cytotoxicity of biocomp.tible QD made of CdSe metal core and ZnS shell with three different coatings and at three different wavelengths （530, 580 and 620 nm）. In terms of half- maximal inhibitory concentration, QSA-QDs with amine-p.lyethyleneglycol coating and QSH-QDs with amp.ip.ilic p.lymer coating were not cytotoxic, while QEI-QDs with p.lyethylenimine coating were highly toxic to the HaCaT cells in comp.rison to a reference CulnS2/ZnS. QEI-QDs led to significant increase in reactive oxygen sp.cies, decrease in mitochondrial membrane p.tential and DNA damage in HaCaT cells. The mechanisms of toxicity of QEI-530 and QEI-580 can be attributed to the combination of intracellular reactive oxygen sp.cies p.oduction and loss of MMp. The QDs toxicity can be attributed to the p.lyethylemimine surface coating which was highly toxic to cells in comp.rison with amine-p.lyethyleneglycol, but not due to the release of cadmium ions.

关键词： nanop.rticles quantum dots HaCaT cytotoxicity genotoxicity

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