检索结果-南通市图书馆

Investigating the Z'gauge boson at future lepton colliders

在线全文

学校读者我要写书评

暂无评论

Chinese Physics C 2022年第5期46卷 52-59页

作者： Xinyue Yin Honglei Li Yi Jin Zhilong Han zongyang lu School of Physics and Technology University of JinanJinan 250022China Guangxi Key Laboratory of Nuclear Physics and Nuclear Technology Guangxi Normal UniversityGuangxi 541004China

Z′boson as a new gauge boson has been proposed in many new physics *** interactions of Z′coupling to fermions have been studied in detail at the large hadron collider.A Z′with the mass of a few TeV has been excluded in some special *** lepton colliders will focus on the studies of Higgs physics,which provide the advantage to investigate the interactions of the Higgs boson with the new gauge *** investigate the Z′ZH interaction via the process of e^(+)e^(-)→Z′/Z→ZH→l^(+)l^(−)bb¯.The angular distribution of the final leptons decaying from the Z-boson is related to the mixing of Z′-Z and the mass of Z′.The forward-backward asymmetry is proposed as an observable to investigate Z′-Z *** angular distributions change significantly with some special beam polarization compared with the unpolarized condition.

关键词： Z',new physics lepton collider

Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9(SaCas9)

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2019年第21期64卷 1592-1600页

作者： Yajing Liu Jianan Li Changyang Zhou Bin Meng Yu Wei Guang Yang zongyang lu Qingmei Shen Yu Zhang Hui Yang Yunbo Qiao School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Institute of Biochemistry and Cell Biology Chinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Institute of Neuroscience State Key Laboratory of NeuroscienceKey Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Shanghai Institute for Advanced Immunochemical Studies ShanghaiTech UniversityShanghai 200031China Precise Genome Engineering Center School of Life SciencesCuangzhou UniversityGuangzhou 510006China

Allele-specific DNA methylation is the most important imprinting marker localized to differentially methylated regions(DMRs),and aberrant genomic imprinted DNA methylation is associated with some human diseases,including Prader-Willi syndrome and ***,the development of an effective strategy for the precise editing of allele-specific methylated genes is essential for the functional clarification of imprinting elements and the correction of imprinting disorders in human *** discover a feasible allele-specific genome editing tool based on the CRISPR/Cas system,which is an efficient genetargeting technique in various organisms,we examined the targeting efficiency of Staphylococcus aureus Cas9(SaCas9)and Streptococcus pyogenes Cas9(SpCas9)in response to DNA methylation *** found that the targeting efficiency of SaCas9,but not SpCas9,was enhanced by targeted DNA demethylation using the d Cas9-Tet1 catalytic domain(CD)but suppressed by targeted DNA methylation using Dnmt3l-Dnmt3a-d *** in vitro cleavage assay further demonstrated that SaCas9 nuclease activity was inhibited by 5-methylcytosine(5mC)in a synthesized Cp G-containing *** analysis with Ch IP-Q-PCR demonstrated that the non-methylated sequence targeting of Sa Cas9 depends on the binding preference of SaCas9 to non-methylated *** advantage of this feature of SaCas9,we have successfully obtained non-methylated allele-biased targeted embryos/mice for two imprinting genes,H19 and Snrpn,with relatively high efficiencies of 28.6%and 47.4%,*** results indicate that the targeting efficiency of SaCas9 was strongly reduced by DNA *** using SaCas9,we successfully achieved allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci.

关键词： Allele-specific DNA methylation SaCas9 Imprinting gene

Epigenomic landscapes during prefrontal cortex development and aging in rhesus

在线全文

学校读者我要写书评

暂无评论

National Science Review 2024年第8期11卷 75-97页

作者： Chao Ning Xi Wu Xudong Zhao zongyang lu Xuelong Yao Tao Zhou Lizhi Yi Yaoyu Sun Shuaishuai Wu Zhenbo Liu Xingxu Huang Lei Gao Jiang Liu National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of Biophysics Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences Division of HIV/AIDS and Sex-Transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC) State Key Laboratory of Drug Regulatory Science Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences Kunming Institute of ZoologyChinese Academy of Sciences School of Life Science and Technology Shanghai Tech University Guangzhou Nvwa Life Technology Co. Ltd Shenzhen Neher Neural Plasticity Laboratory CAS Key Laboratory of Brain Connectome and Manipulation The Brain Cognition and Brain Disease Institute Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Key Laboratory of Epigenetic Regulation and Intervention Institute of Biophysics Chinese Academy of Sciences Zhejiang Provincial Key Laboratory of Pancreatic Disease The First Affiliated Hospital and Institute of Translational Medicine Zhejiang University School of Medicine Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences

The prefrontal cortex(PFC) is essential for higher-level cognitive *** epigenetic dynamics participates in PFC development and aging is ***,we profiled epigenomic landscapes of rhesus monkey PFCs from prenatal to aging *** dynamics of chromatin states,including higher-order chromatin structure,chromatin interaction and histone modifications are coordinated to regulate stage-specific gene transcription,participating in distinct processes of *** changes of epigenetic signals occur around the birth ***,genes involved in neuronal cell differentiation and layer specification are pre-configured by bivalent *** identified a cis-regulatory module and the transcription factors(TFs) associated with basal radial glia development,which was associated with large brain size in *** TFs include GLI3,CREB5 and ***,the genes associated with the basal radial glia(bRG)-associated cis-element module,such as SRY and SOX9,are enriched in sex ***-associated single nucleotide polymorphisms are more enriched in super enhancers(SEs) than typical enhancers,suggesting that SEs play an important role in neural network wiring.A cis-regulatory element of DBN1 is identified,which is critical for neuronal cell proliferation and synaptic neuron ***,the loss of distal chromatin interaction and H3K27me3 signal are hallmarks of PFC aging,which are associated with abnormal expression of aging-related genes and transposon activation,***,our findings shed light on epigenetic mechanisms underlying primate brain development and aging.

关键词： rhesus macaque prefrontal cortex 3D genomics chromatin immunoprecipitation sequencing development aging

Generation of isogenic single and multiplex gene knockout mice by base editing-induced STOP

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2018年第17期63卷 1101-1107页

作者： Guang Yang Tianyu Zhu zongyang lu Guanglei Li Hao Zhang Songjie Feng Yajing Liu Jianan Li Yu Zhang Jia Chen Xuejiang Guo Xingxu Huang School of Life Science and Technology Shanghai Tech University Shanghai 201210 China State Key Laboratory of Reproductive Medicine Department of Histology and Embryology Nanjing Medical University Nanjing 211166 China

Although CRISPR/Cas9 has been widely used to generate knockout mice, two major limitations remain:the founders usually carry a mixture of genotypes, and mosaicism harboring multiple ***, it takes a long time to get homozygous mutants. Recently developed base editing(BE) system,which introduces C-to-T conversion without double strand DNA cleavage, has been used to introduce artificial stop codons(i-STOP) to prematurely terminate translation, providing a cleaner strategy for genome engineering. Using this strategy, we generated CD160 KO and VISTA/CD160 double KO mice by microinjection of a single sg RNA targeting CD160 and a mixture of sg RNAs targeting VISTA and CD160,respectively. The BE system induced STOP efficiently in mouse embryos and consequently in founder mice without detectable off-target. Most interestingly, the majority of the mutants harbor same genetic modifications, indicating we generated isogenic single and multiplex gene mutant mice by BE-induced STOP. We also obtained homozygous mutant mouse in F1 mice, demonstrating the accelerated strategy in generating animal models.

关键词： Base editing i-STOP isogenic Knockout VISTA CDI60

Cynomolgus-rhesus hybrid macaques serve as a platform for imprinting studies

在线全文

学校读者我要写书评

暂无评论

The Innovation 2023年第3期4卷 104-111页

作者： zongyang lu Jie Li Yong lu Ling Li Wei Wang Chenchen Zhang Libing Xu Yanhong Nie Changshan Gao Xinyan Bian Zhen Liu Guang-Zhong Wang Qiang Sun Institute of Neuroscience CAS Center for Excellence in Brain Science and Intelligence TechnologyCAS Key Laboratory of Primate NeurobiologyState Key Laboratory of NeuroscienceChinese Academy of SciencesShanghai 200031China CAS Key Laboratory of Computational Biology Shanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology Shanghai 201210China

Genomic imprinting can lead to allele-specific expression(ASE),where one allele is preferentially expressed more than the *** in genomic imprinting or ASE genes have been widely observed across various neurological disorders,notably autism spectrum disorder(ASD).In this study,we crossed rhesus cynomolgus monkeys to produce hybrid monkeys and established a framework to evaluate their allele-specific gene expression patterns using the parental genomes as a *** proof-of-concept analysis of the hybrid monkeys identified 353 genes with allele-biased expression in the brain,enabling us to determine the chromosomal locations of ASE ***,we confirmed a significant enrichment of ASE genes associated with neuropsychiatric disorders,including ASD,highlighting the potential of hybrid monkey models in advancing our understanding of genomic imprinting.

关键词： imprinting allele chromosomal

Multiple sgRNAs facilitate base editingmediated i-stop to induce complete and precise gene disruption

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2019年第11期10卷 832-839页

作者： Kun Jia zongyang lu Fei Zhou Zhiqi Xiong Rui Zhang Zhiwei Liu Yu'e Ma Lei He Cong Li Zhen Zhu Dejing Pan Zhengxing Lian Beijing Key Laboratory of Animal Genetic Improvement China Agricultural UniversityBeijing 100094China Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research Soochow UniversitySuzhou 215123China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Key Laboratory of MEMS of Ministry of Education Southeast UniversityNanjing 210096China

Dear Editor,Gene editing is a process to introduce desired changes into targeted loci of genomic ***,type II clustered regularly in terspaced short palindromic repeats-associated Cas9 endonuclease(CRISPR/Cas9)system has been demonstrated as a versatile tool for engineering eukaryote genome(Hsu et al.,2014),such as in mice(Zuo et al.,2017).

关键词： CRISPR/Cas9 sgRNA versatile

在线全文

学校读者我要写书评

暂无评论

气动基地式仪表改造的实施策略

仪器仪表用户 2017年第1期24卷 99-102页

作者：芦宗阳宁夏石化公司电仪部银川750001

本文以宁夏石化公司一化肥装置现场气动基地式仪表为对象,介绍气动仪表改造为电动仪表,通过DCS组态实现原有气动基地式仪表控制的功能,着重分析了改造过程中的技术要点、难点及改造方案。

关键词：气动浮筒 CS3000 电动浮筒安全栅液位开关

Three chloroplast chaperonin protomers with various roles constitute authentic oligomers in Chlamydomonas reinhardtii

在线全文

学校读者我要写书评

暂无评论

Three chloroplast chaperonin protomers with various roles co...

不同冲击电流波下金属氧化物避雷器仿真模型适用性研究

2013全国植物生物学大会

作者： BAI Cuicui GUO Peng lu zongyang ZHANG Shijia GAO Fei GAO Liyan WANG Yingchun TIAN Zhixi LIU Cuimin State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory of Molecular Development Biology Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Chloroplast chaperonin is cylinderical tetradecamer composed of two types of subunits. Here we explored the composition and function of chloroplast chaperonin in Chlamydomonas reinhardtii both in vitro and in *** formation of Cpn60βhomo-oligomers was disturbed by Cpn60αsubunits upon co-expression in *** and reconstitution from protomers in *** different behaviors of three protomers indicated their various roles in assembly and the function of oligomers,whose assembly dynamic nature was mediated by *** heterooligomers consisting of three subunits,other than homo-oligomers,functionally interacted with cochaperonin GroES and possessed high substrates refolding *** types of Cpn60 oligomers were separated from Chlamy cells,one was homo-oligomeric Cpn60c localized to mitochondria and the other was hetero-oligomeric Cpn60 consisted of three chloroplast localized subunits which interacted with cochaperonin *** present the authentic chloroplast chaperonin Cpn60 consists of three protomers and Cpn60 assembly dynamic nature contributes to refolding capacity.

关键词： chloroplast chaperonin Cpn60 protein refloding

来源： cnki会议 cnki会议评论

在线全文

学校读者我要写书评

暂无评论

高压电器 2025年第1期61卷 197-203页

作者：陈洁赵丹丹张宗扬陈喆焦婷陆冰冰江安烽田越赵莹莹郭洁国网上海市电力公司电力科学研究院上海200437 华东电力试验研究院有限公司上海200437 西安交通大学电气工程学院西安710049

文中对几种具有代表性的金属氧化物避雷器用电阻片进行了30/60、8/20、4/10、1/5μs 4种冲击电流波下的残压和能量特性实验研究,分析了IEEE、CIGRE、Pinceti与MWR避雷器仿真模型的适用性。结果表明,IEEE模型在1/5μs、幅值5 kA陡波电流... 详细信息

文中对几种具有代表性的金属氧化物避雷器用电阻片进行了30/60、8/20、4/10、1/5μs 4种冲击电流波下的残压和能量特性实验研究,分析了IEEE、CIGRE、Pinceti与MWR避雷器仿真模型的适用性。结果表明,IEEE模型在1/5μs、幅值5 kA陡波电流下残压模拟较差,4种冲击电流波下均可较好的模拟避雷器能量特性;Pinceti、MWR模型在4种冲击电流波下均可较好的模拟避雷器残压特性,Pinceti模型在30/60μs、幅值5 kA的陡波电流下能量模拟较差。文中研究结论可为不同工况下开展MOV仿真时的模型选取与优化提供参考。

关键词：金属氧化物避雷器避雷器模型冲击电流幅值残压特性能量特性