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The Crop Journal 2020年第2期8卷 171-181页

作者： Wei Xi Shuyao tang Haimei Du Jie Luo zongxiang tang Shulan Fu College of Agronomy Sichuan Agricultural UniversityWenjiang 611130SichuanChina Institute of Ecological Agriculture Sichuan Agricultural UniversityWenjiang 611130SichuanChina Provincial Key Laboratory for Plant Genetics and Breeding Wenjiang 611130SichuanChina

Rye(Secale cereale L.)has been widely used to improve wheat(Triticum aestivum L.)*** probes combined with non-denaturing fluorescence in situ hybridization(ND-FISH)technology provide a convenient and efficient way to identify individual rye ***,suitable ND-FISH-positive oligo probes for recognizing specific segments of rye chromosomes are *** new ND-FISH-positive oligo probes:Oligo-5BL.46,Oligo-5A8080,Oligo-5A8080.1,Oligo-1AL.73,and Oligo-0R3,combined with two previously reported oligo probes,Oligo-44 and Oligo-45,were used in this *** Oligo-5BL.46,Oligo-5A8080 and Oligo-44 produced signals only in intercalary regions of arms 1RS,5RS,and 5RL,*** Oligo-5A8080.1 combined with probe Oligo-45 distinguished the intercalary regions of arms 1RS,5RS,and 6RS ***-5A8080 and Oligo-5A8080.1 revealed variation in the distribution of 5S rDNA sequences and polymorphism among 5R *** Oligo-1AL.73 produced signals only on chromosomes 4R and 7R and contributed to the construction of an improved FISH map of chromosome 4RKu and to the confirmation of 4RLKu breakpoints in wheat-rye 4RLKu translocation ***-0R3 produced signals in the telomeric and subtelomeric regions of 14 rye *** oligo probes also revealed five new tandem repeats in *** the oligo probes reported in this study,the short arms of 1R,5R,and 6R and the long arms of 4R and 7R can be easily discriminated when these chromosomes are broken.

关键词： Secale cultivar Probe

Identification and Physical Mapping of New PCR-Based Markers Specific for the Long Arm of Rye(Secale cereale L.) Chromosome 6

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Journal of Genetics and Genomics 2016年第4期43卷 199-206页

作者： Meng Li zongxiang tang Ling Qiu Yangyang Wang Shuyao tang Shulan Fu Province Key Laboratory of Plant Breeding and Genetics Sichuan Agricultural University Agronomy College Sichuan Agricultural University

To effectively use elite genes on the long arm of rye chromosome 6（the 6RL arm） in wheat breeding programs,precise and fast identification of 6RL chromatin in wheat backgrounds is ***-based 6RL-specific markers can facilitate the detection of elite genes on 6RL in wheat ***,only a limited number of 6RL-specific markers have been *** the present study.300 new PCR-based 6RL-specific markers were identified using specific length amplified fragment sequencing（SLAF-seq） technology,and were further physically mapped to four regions on the 6RL arm using 6R and 6RL deletion ***,127 of the 300 markers were physically localized to a region from the site between 2.3 and 2.5 to the telomere,the same region where the powdery mildew resistance gene was *** addition,95 of the 300 markers exhibit polymorphisms,which can be used to investigate the diversity of rye 6RL *** markers developed in this study can be used to identify given segments of 6RL in wheat backgrounds and accelerate the utilization of elite genes on 6RL in wheat breeding.

关键词： Wheat Rye 6RL-specific marker Physical mapping SLAF-seq Powdery mildew

Analysis of DNA methylation variation in wheat genetic background after alien chromatin introduction based on methylation-sensitive amplification polymorphism

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Chinese Science Bulletin 2008年第1期53卷 58-69页

作者： ZHANG Yong LIU ZhaoHui LIU Cheng YANG ZuJun DENG KeJun PENG JinHua ZHOU JianPing LI GuangRong tang zongxiang REN ZhengLong School of Life Sciences and Technology University of Electronic Science and Technology of China Chendu 610054 China Sichuan Provincial Key Laboratory of Plant Breeding and Genetics Sichuan Agriculture University Ya'an 625014 China

During the process of alien germplasm introduced into wheat genome by chromosome engineering, extensive genetic variations of genome structure and gene expression in recipient could be induced. In this study, we performed GISH (genome in situ hybridization) and AFLP (amplified fragment length po-lymorphism) on wheat-rye chromosome translocation lines and their parents to detect the identity in genomic structure of different translocation lines. The results showed that the genome primary struc-ture variations were not obviously detected in different translocation lines except the same 1RS chromosome translocation. Methylation sensitive amplification polymorphism (MSAP) analyses on genomic DNA showed that the ratios of fully-methylated sites were significantly increased in translo-cation lines (CN12, 20.15%; CN17, 20.91%; CN18, 22.42%), but the ratios of hemimethylated sites were significantly lowered (CN12, 21.41%; CN17, 23.43%; CN18, 22.42%), whereas 16.37% were fully-me-thylated and 25.44% were hemimethylated in case of their wheat parent. Twenty-nine classes of me-thylation patterns were identified in a comparative assay of cytosine methylation patterns between wheat-rye translocation lines and their wheat parent, including 13 hypermethylation patterns (33.74%), 9 demethylation patterns (22.76%) and 7 uncertain patterns (4.07%). In further sequence analysis, the alterations of methylation pattern affected both repetitive DNA sequences, such as retrotransposons and tandem repetitive sequences, and low-copy DNA.

关键词：小麦染色体易位排列胚芽 DNA

TRPV1 and PLC involved in Histamine H4 Receptor-Induced Itch

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TRPV1 and PLC involved in Histamine H4 Receptor-Induced Itch

利用小麦-黑麦1BL.1RS易位系快速筛选F_(2)群体中携带抗条锈病基因Yr15的纯合个体

中国生理学会张锡钧基金会第十三届全国青年优秀生理学学术论文交流及评奖会议、中国生理学会第十一届全国青年生理学工作者学术会议

作者： Guanyi Wu Tunyu Jian Niuniu Yang zongxiang tang College of Basic Medicine Guangxi University of Chinese Medicine College of Basic Medicine Nanjing University of Chinese Medicine

Histamine H4 receptor has been confirmed to play a role in evoking peripheral ***,the ionic and intracellular signaling mechanism of activation of H4 receptor on the dorsal root ganglion（DRG） neurons is still *** using cell culture and calcium imaging,we studied the underlying mechanism of activation of H4 receptor on the DRG *** dihydrobromide（Immepip）—a histamine H4 receptor special agonist under cutaneous injection—obviously induced itch behavior of ***-induced scratching behavior could be blocked by TRPV1 antagonist AMG9810 and PLC pathway inhibitor,*** of immepip（8.3-50 uM） could also induce a dose-dependent increase in intracellular Ca([Ca]i) of DRG *** found that 77.8%of the immepip-sensitized DRG neurons respond to the TRPV1 selectively agonist capsaicin.U73122 could inhibit immepip-induced Ca *** addition,[Ca]i the increases from the induction of immepip could be blocked by ruthenium red,capsazepine,and AMG9810,but could not be blocked by TRPA1 antagonist *** results indicate that TRPV1 but not TRPA1 is the important ion channel to induce the DRG neurons' responses in the downstream signaling pathway of histamine H4 receptor,and suggest that TRPV1 may ne involved in the mechanism of histamine-induced itch response by H4 receptor activation.

关键词： Histamine H4 receptor TRPV1 PLC

来源： cnki会议评论

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四川农业大学学报 2023年第6期41卷 1015-1019页

作者：邹杨符书兰唐宗祥四川农业大学农学院/四川农业大学植物遗传育种省级重点实验室成都611130

【目的】为了从F_(2)分离群体中快速鉴定含Yr15基因的纯合个体,从而减少后续选择的工作量。【方法】利用感条锈病的小麦-黑麦1BL.1RS易位系与含Yr15基因的小麦品系杂交,利用基于寡核苷酸探针Oligo-1RNOR的简便NDFISH方法对F_2群体进行... 详细信息

【目的】为了从F_(2)分离群体中快速鉴定含Yr15基因的纯合个体,从而减少后续选择的工作量。【方法】利用感条锈病的小麦-黑麦1BL.1RS易位系与含Yr15基因的小麦品系杂交,利用基于寡核苷酸探针Oligo-1RNOR的简便NDFISH方法对F_2群体进行鉴定。【结果】可以快速筛选出不含1BL.1RS易位染色体的植株,这些植株含1对1B染色体。因1BS不与1RS发生重组,所以它们都是含有Yr15基因的纯合个体,他们的自交后代不会出现抗感分离。【结论】这一方法可以提高Yr15的应用效率。对于其他小麦染色体携带的抗病基因,也可以采用这一方式,利用不同的小麦-黑麦易位染色体,从分离世代中快速鉴定出含抗病基因的纯合个体。该方法有利于加快小麦育种进程。

关键词：小麦条锈病 Yr15 1BL.1RS ND-FISH

新型小麦-黑麦6R附加系的创制及其白粉病抗性基因向小麦中的渗进

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麦类作物学报 2014年第1期34卷 34-38页

作者：李方安唐宗祥符书兰四川农业大学植物遗传育种省级重点实验室四川农业大学农学院成都温江611130

用黑麦(Secale cereale L.)Kustro与普通小麦(Triticum aestivum L.)绵阳11杂交,获得了小麦-黑麦双二倍体材料MK-25,再用小麦品种绵阳11和川农27与MK-25回交,从回交后代中获得了6R单体附加系、6RL端体附加系及来源于6R单体附加系的小片... 详细信息

用黑麦(Secale cereale L.)Kustro与普通小麦(Triticum aestivum L.)绵阳11杂交,获得了小麦-黑麦双二倍体材料MK-25,再用小麦品种绵阳11和川农27与MK-25回交,从回交后代中获得了6R单体附加系、6RL端体附加系及来源于6R单体附加系的小片段渐渗系,且这些附加系和小片段渐渗系都表现出了对白粉病免疫的特性。研究结果表明,来自Kustro的6RL染色体臂上带有白粉病抗性基因,该基因已渐渗入小麦遗传背景中,且能在不同小麦背景中正常表达。因来自Kustro的白粉病抗性基因在前人的研究中尚未见报道,推测该基因可能为新的等位基因。

关键词：小麦黑麦 6R附加系白粉病抗性基因

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中药药性中“苦味”形成机制及生物学意义

广西师范大学学报（自然科学版） 2022年第5期40卷 324-331页

作者：吕佳桦朱婵唐宗湘南京中医药大学医学院·整合医学学院江苏南京210023

“苦”是一种常见的味觉感受,也是中药药性的主要特征;“苦味”作为中药药性“五味”之一,是对中药药物功效和作用规律的高度概括;苦药“能泻、能燥、能坚”,具有“泄火、降气、燥湿和坚阴”等作用;苦味中药占比较高,传统中药理论认为... 详细信息

“苦”是一种常见的味觉感受,也是中药药性的主要特征;“苦味”作为中药药性“五味”之一,是对中药药物功效和作用规律的高度概括;苦药“能泻、能燥、能坚”,具有“泄火、降气、燥湿和坚阴”等作用;苦味中药占比较高,传统中药理论认为苦味成分就是苦味中药的活性成分,这些成分主要包括生物碱、苷类和苦味质等;感受苦味的受体分子分布在味蕾、肠道和呼吸道等组织器官中。本文综述苦味中药的活性物质成分、苦味分级、苦味产生的机制、苦味与疾病之间的关系和苦味的生物学意义等,期望它能对中药药性的研究和苦味中药的临床应用提供参考。

关键词：苦味中药药性苦味受体苦药功能苦的生物意义

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“视觉生理”一节多媒体课件的制作

广西高教研究 2001年第3期 59-61,95页

作者：唐宗湘广西师范大学生物系广西桂林541004

本文以“视觉生理”

关键词：多媒体课件 “视觉生理” 制作方法计算机辅助教学教学改革多媒体素材

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白芷水提液镇痛作用及其机理研究

广西师范大学学报（自然科学版） 2019年第4期37卷 103-110页

作者：郭珺陈丹朱婵唐宗湘南京中医药大学医学与生命科学学院

采用浸尾实验、热辐射实验、辣椒素和福尔马林脚掌致痛实验以及Von-Frey动物行为学实验,探究白芷水提液(ADE)的镇痛作用,并通过原代细胞培养和钙成像技术探讨白芷水提液的镇痛作用机理。结果发现,白芷水提液可有效抑制热浴浸尾和热辐射... 详细信息

采用浸尾实验、热辐射实验、辣椒素和福尔马林脚掌致痛实验以及Von-Frey动物行为学实验,探究白芷水提液(ADE)的镇痛作用,并通过原代细胞培养和钙成像技术探讨白芷水提液的镇痛作用机理。结果发现,白芷水提液可有效抑制热浴浸尾和热辐射引起的伤害性热痛以及由辣椒素或福尔马林诱导的疼痛。然而,在Trpv1基因敲除小鼠中,白芷水提液不能缓解因热刺激或辣椒素引起的小鼠疼痛行为。此外,在小鼠背根神经节神经元中,白芷水提液能显著抑制由辣椒素刺激引起的钙离子内流。可见白芷水提液具有显著的镇痛作用,其镇痛作用与抑制TRPV1通道功能紧密相关。

关键词：白芷镇痛 TRPV1 热痛急性痛

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神经源性炎症痛的分子机制及研究进展

中国疼痛医学杂志 2022年第7期28卷 486-493页

作者：孙建梅刘彤彤唐宗湘南京中医药大学医学院·整合医学学院南京210023

神经源性炎症是指由感觉神经元引起的炎症,即当感觉神经元受到伤害性刺激时,所产生的动作电位会顺向传递到中枢神经系统(背根反射),或者逆向传递到感觉神经末梢(轴突反射),进而影响突触或者轴突末梢释放神经肽(如降钙素基因相关肽和P物... 详细信息

神经源性炎症是指由感觉神经元引起的炎症,即当感觉神经元受到伤害性刺激时,所产生的动作电位会顺向传递到中枢神经系统(背根反射),或者逆向传递到感觉神经末梢(轴突反射),进而影响突触或者轴突末梢释放神经肽(如降钙素基因相关肽和P物质),最终引起神经系统或外周组织的炎症反应。神经源性炎症痛则是由于神经源性炎症敏化了伤害性神经末梢而导致的疼痛。神经源性炎症痛涉及多种以疼痛为主要临床症状的疾病机制。因此,探究神经源性炎症痛的分子机制对多种以疼痛为主要临床表现的疾病治疗具有重要意义。本文就神经源性炎症痛的定义、发生机制、临床诊断及治疗以及神经源性炎症痛伴发的相关疾病进行了综述。

关键词：神经源性炎症炎性痛分子机制治疗策略