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Science China Chemistry 2023年第7期66卷 1905-1923页

作者： Dan Duan Xiaohan Cai Cong Ma zongxi lin Yao Wang Jianwei Nai Tiefeng Liu Jianmin Luo Yujing Liu Xinyong Tao College of Materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014China

Lithium-ion batteries(LIBs)have been in a dominant position in the new energy industry because of their excellent comprehensive performance.The performance of LIBs highly depends on the microstructures of the materials that constitute LIBs.Particularly,the relatively“weak”molecular interactions instead of the common-discussed strong chemical-bonding always affect the structures and the consequent properties of the components in LIBs.As a typical example,the hydrogen bonds,which widely exist inside LIBs,greatly improved the mechanical strength,lithium ion(Li^(+))transport rate and the intrinsic stabilities towards boosting performance of LIBs.This review starts from the interaction force between molecules,and especially summarizes the correlation between the formation of hydrogen bonds and the properties of the typical components in LIBs(cathode,anode,electrolyte,separator).In addition,how the formation of hydrogen bonds affects the performance of LIBs components is discussed.Finally,the strategies of combining hydrogen bonds with LIBs components in the future are prospected,which provide guidance for the rational design of high-performance LIBs.

关键词： hydrogen bonds lithium-ion batteries electrochemical performance

Solid polymer electrolytes in all-solid-state lithium metal batteries:From microstructures to properties

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能源化学:英文版 2023年第6期81卷 358-378,I0009页

作者： zongxi lin Ouwei Sheng Xiaohan Cai Dan Duan Ke Yue Jianwei Nai Yao Wang Tiefeng Liu Xinyong Tao Yujing Liu College of Materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014ZhejiangChina

All-solid-state lithium(Li)metal batteries(ASSLMBs)are considered one of the most promising secondary batteries due to their high theoretical capacity and high safety performance.However,low room-temperature ionic conductivity and poor interfacial stability are two key factors affecting the practical application of ASSLMBs,and our understanding of the mechanisms behind these key problems from microscopic perspective is still limited.In this review,the mechanisms and advanced characterization techniques of ASSLMBs are summarized to correlate the microstructures and properties.Firstly,we summarize the challenges faced by solid polymer electrolytes(SPEs)in ASSLMBs,such as the low roomtemperature ionic conductivity and the poor interfacial stability.Secondly,several typical improvement methods of polymer ASSLMBs are discussed,including composite SPEs,ultra-thin SPEs,SPEs surface modification and Li anode surface modification.Finally,we conclude the characterizations for correlating the microstructures and the properties of SPEs,with emphasis on the use of emerging advanced techniques(e.g.,cryo-transmission electron microscopy)for in-depth analyzing ASSLMBs.The influence of the microstructures on the properties is very important.Until now,it has been difficult for us to understand the microstructures of batteries.However,some recent studies have demonstrated that we have a better understanding of the microstructures of batteries.Then we suggest that in situ characterization,nondestructive characterization and sub-angstrom resolution are the key technologies to help us further understand the batteries'microstructures and promote the development of batteries.And potential investigations to understand the microstructures evolution and the batteries behaviors are also prospected to expect further reasonable theoretical guidance for the design of ASSLMBs with ideal performance.

关键词： Lithium metal batteries Solid polymer electrolytes Microstructures Properties

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“诗穷而后工”的文化现象阐释

陇东学院学报 2024年第3期35卷 29-31页

作者：丁琳天水师范学院文学与文化传播学院甘肃天水741000

中国古代的文学思想——“诗穷而后工”,即穷困激发文学才华的理论由欧阳修提出,韩愈、陆游等人对其继承发展,再到黄宗羲将其提高到诗歌“原本”的高度。这一理论的内涵是受压迫者的不平之气,与人民的愤懑和反抗精神是相通的。作家必须... 详细信息

中国古代的文学思想——“诗穷而后工”,即穷困激发文学才华的理论由欧阳修提出,韩愈、陆游等人对其继承发展,再到黄宗羲将其提高到诗歌“原本”的高度。这一理论的内涵是受压迫者的不平之气,与人民的愤懑和反抗精神是相通的。作家必须在不同程度上接触和体验人民的生活,只有深刻反映生活真实的作品才能达到艺术上的“工”。

关键词： “诗穷而后工” 理论文学精神

Advances of Studies on the Viral Proteins of PRRSV

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Animal Husbandry and Feed Science 2014年第2期6卷 80-82,90页

作者： Cao zongxi Shi Zhihai lin Zhemin Jiao Peirong Zhang Guihong Hainan Provincial Key Laboratory of Tropical Animal Reproduction & Breeding and Veterinary Medicine Institute of Animal Husbandry and Veterinary Medicine of Hainan Academy of Agricultural Sciences Key Laboratory for Animal Vaccine Development of Ministry of Agriculture South China Agricultural University Institute of Animal Husbandry and Veterinary Medicine Henan Academy of Agricultural Sciences

Porcine reproductive and respiratory syndrome( PRRS) is one of viral diseases with severe reproductive obstacle of pregnant sows and respiratory tract symptoms and higher mortality of piglets as characteristics,which is caused by porcine reproductive and respiratory syndrome virus( PRRSV). PRRS has brought great threats to swine industry in the world. The advances of studies on the viral proteins of PRRSV were reviewed from the genome,non-structural proteins and structural proteins of PRRSV.

关键词： PRRSV Viral genome Non-structural proteins Structural proteins

Study on Antibody Dynamics against Porcine Contagious Pleuropneumonia in Piglets

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Agricultural Biotechnology 2018年第1期7卷 66-69页

作者： Hailong LIU Yan ZHANG Lili HUANG zongxi CAO Zhemin lin Institute of Animal Husbandry and Veterinary Medicine Hainan Academy of Agricultural Sciences Haikou 571100 China Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research Haikou 571100 China

In order to provide basis for establishing a scientific and reasonable immune procedure against porcine contagious pleuropneumonia in pig farms, antibody dynamics in piglets inoculated with two vaccines against porcine contagious pleuropneumonia （ APP-1,2, 7 ） were detected. The result showed that average antibody blocking rate and antibody positive rate in piglets inoculated with two vaccines against porcine contagious pleuropneumonia exhibited similar variation trend. At 21 day after primary vaccination, average antibody blocking rate and antibody positive rate against porcine contagious pleuropneumonia in piglets reached the peak and then declined gradually. After secondary vaccination, average antibody blocking rate and antibody positive rate against porcine contagious pleuropneumonia in piglets increased gradually, which reached the second peak at 30 day after secondary vaccination （60 day after primary vaccination） ; subsequently, average antibody blocking rate declined gradually but still remained at a high level; antibody positive rate had little change. Average antibody blocking rate against APP-1 was slightly lower than the critical level of antibody protection at 44 day after primary vaccination ; antibody positive rate was slightly higher than the qualification rate at 37 day after primary vaccination; average antibody blocking rate against APP-7 was close to the critical level of antibody protection at 30 day after primary vaccina- tion, suggesting that piglets should be inoculated twice with polyvaccine against porcine contagious pleuropneumonia during the entire growth period, and the secondary vaccination should be performed at 30 -37 day after primary vaccination.

关键词： Piglet Porcine contagious pleuropneumonia Immune antibody Dynamics Immune procedure

Study on Growth and Decline Law of Contagious Pleuro-pneumonia in Piglets

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Agricultural Biotechnology 2018年第5期7卷 116-118页

作者： Yan ZHANG Hailong LIU Zhemin lin zongxi CAO Shuyi TAN Institute of Animal Husbandry and Veterinary Medicine Hainan Academy of Agricultural SciencesHaikou 571100China Hainan Key Laboratory for Tropical Animal Breeding and Disease Research Haikou 571100China

In order to establish the immune procedure for Actinobacillus pleuropneumonia and to determine the date for the first immunization on piglets, the growth and decline law of A. pleuropneumonia material antibodies in the piglets borne by cows inoculated with A. pleuropneumonia vaccines （type I, II, and VII） before delivery was detected. The results showed that type I, II, and VII maternal antibodies in piglets decreased gradually with the age growing overall, and was at the critical protection value at the ages of 42-50 days （type I） and 28 days （type VII）, lower than the quantification rate; and the antibodies all turned to be negative until the ages of 70 days （type I）, 60 days （type VII） and 35 days （type II）. The first immunization should be carried out at the age of 42-50 days using type I A. pleuropneumonia vaccine, and at the age of 28 days using type VII A. pleuropneumonia vaccine. However, type II A. pleuropneumonia maternal antibody had lower level and positive rate and could not well protect piglets, so the various A. pleuropneumonia vaccines differed in the date for the first immunization. In order to achieve a better immunization effect, A. pleuropneumonia vaccines with different valences should be further researched and developed.

关键词： Porcine contagious pleuropneumonia Maternal antibody Growth and decline law Piglets

Isolation and Sequence Analysis of NSP2, ORF3 and ORF5 of PRRSV XH-GD Strain from Guangdong Province

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Agricultural Biotechnology 2014年第1期3卷 4-8页

作者： zongxi CAO Quanhui PAN Zhemin lin Liuwu KONG Yimin HE Guihong ZHANG Hainan Provincial Key Laboratory of Tropical Animal Reproduction and Breeding and Veterinary Medicine Institute of Animal Husbandry and Veterinary MedicineHainan Academy of Agricultural Sciences MOA Key Laboratory for Animal Vaccine Development South China Agricultural University

The diseased samples collected from Guangdong Province were inoculated to Marc-145 cells, PRRSV-specific cytopathogenic effects （CPE） such as cell aggregation, cell shrinkage and cell exfoliation were observed and a PRRSV strain XH-GD was obtained. By using primers designed in accordance with the pub- lished gene se^lences of PRRSV in GenBank, NSP2, ORF3 and ORF5 fragments were amplified by reverse transcription polymerase chain reaction and analyzed by sequence aligment. The results demonstrated that PRRSV strain XH-GD belonged to the North American genotype and the NSP2 gene contained discontinuous de- letions of 30 amino acids. NSP2, ORF3 and ORF5 genes of XH-GD shared 83.3% -98.9%, 88.6% -99.2% and 88.1% -99.2% nucleotide homology, and 76.8% -98.3%, 83.7% -98.8% and 88.1% -99.2% amino acid homology with HUB1, NX06, BJsy06, VR-2332, HB-1（sh）2002, HB-2（sh）2002, CH-1a and RespPRRS MLV, respectively; however, NSP2, ORb3 and ORF5 genes of XH-GD shared only 52.9%, 65.0% and 64. 3% nucleotide homology, and 31.3%, 57.5% and 58.9% amino acid homology with LV, respectively. Phylogenetic analysis revealed that XH-GD had a closer relationship with HUB1, NX06, BJsy06, VR-2332, HB-1 （sh） 2002, HB-2 （sh） 2002, CH-la and RespPRRS MLV strains compared with LV strain.

关键词： PRRSV XH-GD strain Sequence analysis

Comparison of Immune Effect of Four Commercial Marek's Disease Vaccines in Wenchang Chicken

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Animal Husbandry and Feed Science 2015年第1期7卷 18-20页

作者： Cao zongxi Zhang Yan Tan Shuyi Ye Baoguo lin Zhemin Institute of Animal Husbandry and Veterinary Medicine Hainan Academy of Agricultural Sciences

Marek＇s disease（MD） is a lymphoproliferative disease of domestic chickens caused by a highly infectious,oncogenic alpha-herpesvirus known as Marek＇s disease virus（MDV）.The aim of this study was to compare the efficacy of four commercial MDV vaccines in Wenchang chicken.The 1-day old Wenchang chickens tested were injected with one of four different vaccines or not unvaccinated as control;five days later,they were then challenged by virulent MDV strain MD5.The results showed that,in comparison with HVT vaccines,the CVI988 vaccine gave the immunized chickens more potent immunities against challenges of MDV strain MD5.

关键词： Marek＇s disease virus Wenchang chicken Immunity efficacy Comparative trial

Phylogenetic Relationship Analysis of the Complete Genomes of Porcine Circovirus Type 2( PCV2) Strains Isolated from Hainan Province

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Agricultural Biotechnology 2015年第5期4卷 45-48,53页

作者： Baoguo YE Xinli ZHENG Yan ZHANG Zhemin lin Feng WANG zongxi CAO Institute of Animal Husbandry and Veterinary Medicine Hainan Academy of Agricultural Sciences

[ Objective] This study aimed to investigate the molecular characteristics of porcine circovirus type 2 （PCV2） strains isolated from Hainan Province. [ Method] The complete genome of PCV2 was amplified from PMWS-suspected samples by PCR for sequence analysis. [ Result] A total of eight PCV2 strains were isolated and identified. All the eight isolates belonged to genotype PCV2b, among which seven isolates belonged to subgenotype PCV2b-1 C, and one isolate be- longed to subgenotype PCV2b-IA/1B. ORF2 gene of PCV2 isolates from Hainan Province was 705 bp in length, encoding 234 amino acids. Antigenic epitopes of Cap protein exhibited certain changes. Nucleotide sequences and deduced amino acid sequences of ORF2 gene shared 95.3% -99.7% and 93.6% - 100% simi- larities among eight PCV2 isolates from Hainan Province, respectively. Moreover, nucleotide sequences and deduced amino acid sequences of ORF2 gene of PCV2 isolates from Hainan Province shared 91.0% -99.9% and 91.0% -99.6% similarities with other PCV2 strain isolated from China （AY682994, AF381175, JX945577, JX682407, AY180397 ）, respectively; nucleotide sequences and deduced amino acid sequences of ORF2 gene of PCV2 isolates from Hainan Province shared 90.0% - 97.0% and 88.0% -97.9% similarities with PCV2 isolates from other countries （ NC_005148, JQ994268, KJ187306, AF201307, AF454546, AY＂/Sd020）, respectively; nucleotide sequences and deduced amino acid sequences of ORF2 gene of PCV2 isolates from Hainan Province shared 98.2% -100% and 94.9% -100% similarities with vaccine strain SH, respectively; nucleotide sequences and deduced amino acid sequences of ORF2 gene of PCV2 isolates from Hainan Province shared 90.6% -91.7% and 89.7% - 91.0% similarities with vaccine strain LG, respectively. [ Conclusion] This study provided theoretical hasis for the prevention and control of PCV2 and selection of vaccine strains in Hainan Province.

关键词： Porcine circovirus type 2 Complete genome Cloning Sequence analysis

Effects of Compound Acidifier on Growth Performance and Serum Biochemical Parameters of Heat-stressed Wenchang Chickens

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Animal Husbandry and Feed Science 2020年第2期12卷 39-42,44页

作者： Liu Quanwei Gu Lihong Xing Manping lin Dajie Liu Hailong Cao zongxi Jiang Shouqun lin Xiajing lin Zhemin Institute of Animal Science and Veterinary Medicine Hainan Academy of Agricultural Sciences/Hainan Key Lab of Tropical Animal ReproductionBreeding and Epidemic Disease ResearchHaikou 571100China Institute of Animal Science Guangdong Academy of Agricultural SciencesGuangzhou 510640China

[Objective]The paper was to investigate the effects of compound acidifier on growth performance and serum biochemical parameters of heat-stressed Wenchang chickens.[Method]A total of 28881-day-old Wenchang chickens with the same genetic background and similar initial weight(985.63±20.35 g)were randomly divided into four groups,four replicates per group and 18 chickens per replicate.Birds were fed the basal diet supplemented with 0 g/kg(control group),1 g/kg(groupⅠ),2 g/kg(groupⅡ)and 3 g/kg(groupⅢ)compound acidifier.The trial lasted 35 d.[Result]The daily gain of experimental groupⅠwas significantly higher than those of control group and groupⅢ(P0.05),but the serum uric acid concentration decreased(P>0.05).[Conclusion]Supplementing 1 g/kg compound acidifier could improve the growth performance of heat-stressed Wenchang chickens.

关键词： Compound acidifier Heat-stressed Wenchang chickens Growth performance Serum biochemical parameters