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BIOCELL 2022年第7期46卷 1751-1762页

作者： JIANMIN PAN XIN LI JUANJUAN LI HUI LIU ZHUONI HOU zongqi yang LING XU Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou310018China UWA School of Agriculture and Environment and The UWA Institute of Agriculture Faculty of ScienceThe University of Western AustraliaPerth6009Australia

Plant U-box(PUB)E3 ubiquitin ligases play important roles in hormone signaling pathways and in response to different abiotic stresses,but little is known about U-box genes in Danshen(root of Salvia miltiorrhiza Bunge).Here,we identified and characterized 70 SmPUB genes based on its genome *** analysis of U-box genes from *** and Arabidopsis suggested that they can be clustered into seven subgroups(I–VII).Typical U-box domains were found in all identified SmPUB genes through the analysis of conserved ***,qRT-PCR was applied to analyze the relative expression levels of U-box genes in *** roots and leaves under PEG-induced water deficit and salt *** revealed that the SmPUB genes exhibited stronger response to drought than to salt *** the best of our knowledge,this report is the first to perform genome-wide identification and analysis of the U-box gene family in ***,and the results provide valuable information for better understanding of the function of U-box in ***.

关键词： Salvia miltiorrhiza U-box E3 ubiquitin ligase Gene-family Abiotic stresses Expression pattern

Genome-wide identification of NAC gene family and expression analysis under abiotic stresses in Salvia miltiorrhiza

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BIOCELL 2022年第8期46卷 1947-1958页

作者： XIN LI JIANMIN PAN FAISAL ISLAM JUANJUAN LI ZHUONI HOU zongqi yang LING XU Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou310018China Institute of Crop Science Ministry of Agriculture and Rural Affairs Laboratory of Spectroscopy SensingZhejiang UniversityHangzhou310058China

NAC(NAM,ATAF,CUC)is a class of transcription factors involved in plant growth regulation,abiotic stress responses,morphogenesis and *** miltiorrhiza is an important Chinese medicinal herb,but the characterization of NAC genes in this species is *** this study,based on the Salvia miltiorrhiza genomic databases,82 NAC transcription factors were identified,which were divided into 14 ***,phylogenetic analysis,gene structure,chromosomal localization and potential role of SmNACs in abiotic stress conditions were also *** results revealed that some SmNACs had different structures than others,which advised that these genes may have multiple/distinct ***-time quantitative polymerase chain reaction(RT-qPCR)analysis showed that SmNACs exhibited differential expression patterns under salt and drought *** NaCl induced salinity treatments modulated the expression of several SmNAC genes more in roots compared with ***,under drought stress conditions,more genes were upregulated in leaves compared with *** results will be useful for the further study involved in the functional characteristics of SmNAC genes,especially in response to salt and drought stresses,thereby may facilitate genetic breeding in Salvia miltiorrhiza.

关键词： Salvia miltiorrhiza Arabidopsis thaliana NAC transcription factors Genome-wide analysis Abiotic stress

Germplasm resources and secondary metabolism regulation in Reishi mushroom(Ganoderma lucidum)

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Chinese Herbal Medicines 2023年第3期15卷 376-382页

作者： Xinyu He Yiwen Chen Zhenhao Li Ling Fang Haimin Chen Zongsuo Liang Ann Abozeid zongqi yang Dongfeng yang Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou 310018China Zhejiang Shouxiangu Botanical Drug Institute Co. Ltd.Hangzhou 310018China Shaoxing Academy of Biomedicine Co. Ltd.of Zhejiang Sci-Tech UniversityZhejiang Engineering Research Center for Development Technology of Medicinal and Edible Health FoodShaoxing 312000China Botany and Microbiology Department Faculty of ScienceMenoufia UniversityShebin Elkoom 32511Egypt

Ganoderma lucidum is a valuable medical macrofungus with a myriad of diverse secondary metabolites,in which triterpenoids are the major *** paper introduced the germplasm resources of genus Ganoderma from textual research,its distribution and identification at the molecular *** we overviewed *** in the components,the biological activities and biosynthetic pathways of ganoderic acid,aiming to provide scientific evidence for the development and utilization of *** germplasm resources and the biosynthesis of ganoderic acid.

关键词： chemical constituents Ganoderma lucidum(Leyss.ex Fr.)Karst. ganoderic acid germplasm resources pharmacological activities secondary metabolism regulation

Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast

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Chinese Science Bulletin 2006年第14期51卷 1703-1709页

作者： yang zongqi LI yinü CHEN Feng LI Dong ZHANG Zhifang LIU Yanxin ZHENG Dexian WANG Yong SHEN Guifang College of Life Sciences Nankai University Tianjin 300071 China Biotechnology Research Institute Chinese Academy of AgriculturalSciences Beijing 100081 China National Laboratory of Medical Molecular Biology Institute of BasicMedical Sciences Chinese Academy of Medical Sciences and PekingUnion Medical College Beijing 100005 China

Tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expres-sion vector, p64TRAIL, containing the cDNA coding for the soluble TRAIL (sTRAIL), was constructed with clpP-trnL-petB-chlL-rpl23-rpl2 as Chlamydomonas reinhardtii plastid homologous recombinant frag-ments and spectinomycin-resistant aadA gene as a select marker. The plasmid p64TRAIL was trans-ferred into the chloroplast genome of C. reinhardtii by the biolistic method. Three independently trans-formed lines were obtained by 100 mg/L spectino-mycin selection. PCR amplification, Southern blot analysis of the sTRAIL coding region DNA and culti-vation cells in the dark all showed that the exogenous DNA had been integrated into chloroplast genome of C. reinhardtii. Western blot analysis showed that human soluble TRAIL was expressed in C. reinhardtii chloroplast. The densitometric analysis of Western blot indicated that the expressed human sTRAIL protein in the chloroplasts of C. reinhardtii accounted for about 0.43%―0.67% of the total soluble proteins. These experimental results demonstrated the possi-bility of using transgenic chloroplasts of green alga as bioreactors for production of biopharmaceuticals.

关键词： TRAIL 叶绿体同源重组体衣藻

Characteristics of the Middle-Late Triassic sedimentary facies assemblages in the Songpan-Ruoergai area

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Progress in Natural Science:Materials International 2007年第3期17卷 305-313页

作者： YAN Zhen, YU Liangjun, LI Jiliang, BIAN Qiantao, WANG zongqi and yang Yongcheng ( Institute of Geology, Chinese Academy of Geological Sciences, Beijing 100037, China State Key Laboratory of Lithospheric Evolution, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China Shaanxi Insititute of Geology Survey, Xianyang 712000, China) [a]Institute of Geology Chinese Academy of Geological Sciences Beijing China [b]State Key Laboratory of Lithospheric Evolution Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China [c]Shaanxi Insititute of Geology Survey Xianyang China

The Middle-Late Triassic sedimentary rocks in the Songpan-Ruoergai area mainly consist of calcareous siltstone, muddy limestone, lithic arkose, feldspathic litharenite, mudstone, wormkalk, oolithic limestone, and conglomerate. Except for limestone bed increasing eastward longitudinally and vertically, the calcareous component of the sandstones increases obviously. Abundant benthic and plant fossils and their clasts occur within these rocks. The sedimentary structures predominately contain flaser, parallel, tabular, wavy, and herringbone cross beddings. These data coevally imply that the Middle-Late Triassic sediments deposited in the fluvial, lake and tide environments. Additionally, the rocks display graded, parallel, hummocky beddings, and sandy lamination, a feature characteristic of storm deposits.

关键词： Middle-Upper Triassic fluvial-lake environments tide environment tempestite Songpan-Ruoergai

面波频散谱多模式高分辨率成像的多道信号比较法

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地球物理学报 2021年第5期64卷 1710-1720页

作者：易佳刘伊克胡昊张郁山杨宗奇中国地震灾害防御中心北京100029 中国科学院地质与地球物理研究所北京100029 University of Houston Houston Texas77204-5007USA 中国石油集团东方地球物理勘探责任有限公司河北涿州072750

高分辨率的面波频散谱成像是浅层地震勘探领域基于频散性质反演横波速度结构中的一个关键步骤.在天然地震探测领域,仅利用两个台站记录的线性信号比较法(LSC),被广泛用来计算面波的频散谱,并用于大尺度的面波层析成像.然而互相关的成像... 详细信息

高分辨率的面波频散谱成像是浅层地震勘探领域基于频散性质反演横波速度结构中的一个关键步骤.在天然地震探测领域,仅利用两个台站记录的线性信号比较法(LSC),被广泛用来计算面波的频散谱,并用于大尺度的面波层析成像.然而互相关的成像方式会造成频散谱在低频端较低的分辨率.非线性信号比较法(NLSC)利用指数函数克服了这个问题,同时极大地提高了频散谱的成像分辨率.然而,在研究中我们发现,仅利用两个台站的地震记录,并不能将面波的频散特性完整地考虑在内,导致LSC和NLSC方法对高阶模式的成像存在较大的误差.由于主动源面波勘探多道采集的方式,基于信号比较理论的多道信号比较法(MSC)充分利用多道地震信号,可以获得准确的多模式成像,然而该方法需要计算任意两道的频散谱并叠加,存在冗余的计算,导致计算效率较低.因此,本研究对MSC方法进行了相应的改进,通过追踪炮集记录上的面波波组提高了原方法的计算效率,同时,利用理论频散曲线进行叠加分析,验证了改进的MSC方法的正确性和有效性.通过与相移法、LSC和NLSC方法的对比分析,展示了MSC方法是一种准确的、高分辨率的面波多模式频散谱成像方法.实际地震资料的应用,揭示了MSC方法在浅层地震勘探中用于提取面波多模式频散信息的巨大潜力.

关键词：浅层地震勘探横波速度面波频散曲线信号比较法

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高等植物叶绿体表达重组蛋白研究进展

生物工程学报 2018年第5期34卷 631-643页

作者：林优红程霞英杨东风梁宗锁杨宗岐浙江理工大学生命科学学院浙江杭州310018

近年来,基因工程技术发展迅速,许多重组蛋白得以表达。其中利用植物生物反应器表达特异药物蛋白为人类一些重要疾病的预防和治疗提供了新途径。植物叶绿体遗传转化和表达系统成为目前植物生物反应器的研究热点。因结构和遗传上的特殊性... 详细信息

近年来,基因工程技术发展迅速,许多重组蛋白得以表达。其中利用植物生物反应器表达特异药物蛋白为人类一些重要疾病的预防和治疗提供了新途径。植物叶绿体遗传转化和表达系统成为目前植物生物反应器的研究热点。因结构和遗传上的特殊性,高等植物叶绿体在重组蛋白表达方面具有独特优势,外源基因表达量高、定点整合,而且叶绿体母系遗传特性保证了生物安全性。很多重要药用蛋白质在植物叶绿体中表达成功。烟草作为高等植物叶绿体转化模式植物,在疫苗抗原、抗体等药物蛋白和其他重要重组蛋白表达方面取得显著进展。高等植物叶绿体遗传转化也为叶绿体基因的表达和调控机制的研究提供新的技术和方法。文中从叶绿体遗传转化原理、载体构建、重组蛋白和重要药物蛋白在叶绿体中的表达以及重组蛋白表达对植物代谢和性状影响等多个角度,对高等植物叶绿体遗传转化体系研究的新进展进行了综述,以期为叶绿体表达平台的开发和重要药用蛋白质的表达提供新思路。

关键词：高等植物叶绿体表达生物反应器重组蛋白

CFRP层间碳纳米管定向喷涂工艺及断裂韧性研究

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复合材料学报 2021年第2期38卷 496-505页

作者：蒋鹏王仲奇常正平杨宗琪周旭冯征昊西北工业大学机电学院西安710072 伯明翰大学冶金与材料学院伯明翰B152TT

为了提高碳纤维增强树脂基复合材料(CFRP)层间性能,采用共沉淀法在碳纳米管上接枝磁性Fe_(3)O_(4)粒子,通过定向喷涂工艺使磁性碳纳米管(Fe_(3)O_(4)-MWCNTs)在碳纤维表面取向一致,并喷涂树脂加以固定,形成碳纤维-定向碳纳米管-树脂界面... 详细信息

为了提高碳纤维增强树脂基复合材料(CFRP)层间性能,采用共沉淀法在碳纳米管上接枝磁性Fe_(3)O_(4)粒子,通过定向喷涂工艺使磁性碳纳米管(Fe_(3)O_(4)-MWCNTs)在碳纤维表面取向一致,并喷涂树脂加以固定,形成碳纤维-定向碳纳米管-树脂界面,采用真空辅助树脂渗透成形(Vacuum assisted resin infusion, VARI)工艺制备层间性能优异的Fe_(3)O_(4)-MWCNTs层间定向增强CFRP。试验结果表明,喷涂树脂可改善和巩固定向喷涂工艺。与未加磁场喷涂工艺相比,当Fe_(3)O_(4)-MWCNTs的质量分数为0.3wt%时,采用定向喷涂工艺试件的I型层间断裂韧性(GIC)提升幅度最大,GIC提高了37.7%。断面形貌分析表明其增强机制以树脂的塑性变形、Fe_(3)O_(4)-MWCNTs棒状聚集体的拔出及树脂塑性孔洞的生长为主。该研究为具有可控定向行为的磁性碳纳米管改性CFRP层间力学性能提供了新思路与方法。

关键词： CFRP 磁性碳纳米管喷涂工艺界面改性断裂韧性

外源5–氨基乙酰丙酸对紫花苜蓿生理特性的影响

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草业科学 2019年第11期36卷 2847-2853页

作者：李丹丹梁宗锁杨宗岐韩蕊莲西北农林科技大学草业与草原学院

以紫花苜蓿(Medicago sativa)"农牧806"为试验材料,通过叶面喷施不同浓度(0、5、10、15、20、25 mg·L^–1)的5–氨基乙酰丙酸(ALA),探讨外源5–氨基乙酰丙酸对紫花苜蓿抗氧化酶活性和光合色素含量的影响。结果表明,叶面喷施10 mg·L^–... 详细信息

以紫花苜蓿(Medicago sativa)"农牧806"为试验材料,通过叶面喷施不同浓度(0、5、10、15、20、25 mg·L^–1)的5–氨基乙酰丙酸(ALA),探讨外源5–氨基乙酰丙酸对紫花苜蓿抗氧化酶活性和光合色素含量的影响。结果表明,叶面喷施10 mg·L^–1 ALA可显著(P<0.05)提高紫花苜蓿幼苗的光合色素含量,与不喷施对照处理相比,紫花苜蓿"农牧806"幼苗叶片中叶绿素a(Chl a)、叶绿素b(Chl b)、类胡萝卜素(Car)以及叶绿素总量(Chl a+b)分别提高了12.29%、36.21%、18.69%和11.46%。喷施10 mg·L^–1 ALA后,紫花苜蓿幼苗叶片中抗氧化酶(SOD、POD、CAT、APX)活性、可溶性蛋白含量显著升高而丙二醛(MDA)和过氧化系统(–OH、H2O2)的含量则显著降低。说明适宜浓度(5~10 mg·L^–1)的ALA可提高紫花苜蓿叶片中光合色素的积累及抗氧化酶活性。

关键词：外源5–氨基乙酰丙酸紫花苜蓿生理特性

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植物学实验教学改革探究

实验科学与技术 2013年第5期11卷 80-81+89页

作者：杨宗岐程霞英浙江理工大学生命科学学院

针对传统植物学实验教学存在的问题,在教学内容、教学方法和考核方法三方面提出了相应的改革措施,旨在提高学生实验技能、学习积极性,增强学生的创新意识和能力。

关键词：植物学实验实验教学实验技能教学改革