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Neuroprotective effects of combined lead and cadmium,as well as N-acetylcysteine,on cerebral cortical neurons following lipid peroxidation injury

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Neural Regeneration Research 2009年第11期4卷 907-911页

作者： Yumei Zhang Hao Lu Yan Yuan Xuezhong liu zongping liu College of Veterinary Medicine Yangzhou University Yangzhou 225009 Jiangsu Province China College of Veterinary Medicine Northwest Sci- Tech University of Agriculture and Forestry Yangling 712100 Shaanxi Province China

BACKGROUND： Studies have reported the antioxidant effects of lead and cadmium in the central nervous system, but very few have addressed the combined toxicity of lead and cadmium. The mechanisms by which these combined heavy metals are toxic, as well as how to protect cells from these agents, remains poorly understood. OBJECTIVE： Primary cultured rat cortical neurons were used to determine the effects of combined lead and cadmium on levels of glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD）, catalase （CAT）, and acetylcholinesterase （ACHE）, as well as malondialdehyde （MDA）, and to evaluate the neuroprotective effects of N-acetylcysteine （NAC）. DESIGN, TIME AND SETTING An in vitro toxicological observation was performed at the Comparative Medicine Center of Yangzhou University from August 2007 to April 2008. MATERIALS： Lead acetate, cadmium acetate, and NAC were purchased from Sigma-Aldrich （St. Louis, USA）. Commercial kits of GSH-Px, SOD, CAT, ACHE, and MDA were purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. METHODS： The cerebral cortical neurons were isolated from newborn Sprague dawley rats at 24 hours after birth and primary cultured for 6 days. Thereafter, the cells were treated with a range of cadmium doses （0, 5.0, and 10.0μmol/L）, lead doses （0, 1.0, and 2.0 μmol/L）, or a combination of the two for 12 hours at 37℃in a 5% CO2 incubator, respectively. In addition, the cells were incubated with different doses of cadmium and/or lead and （0 and 50 μmol/L） NAC for 12 hours to assess the protective effects on cell survival. MAIN OUTCOME MEASURES： The activity of SOD, GSH-Px, CAT, and ACHE, as well as MDA content, in the cell lysates was detected using commercial kits. RESULTS： At 12 hours after treatment, compared to the control group, activity of GSH-Px, SOD, and AChE in the lead, cadmium, or combined treated cells was significantly decreased with increasing doses of cadmium/or lead （P 〈 0.05）, but CAT activity and MDA levels were significantly increased （P 〈 0.05）. The com

关键词： lead cadmium cortical neurons N-acetylcysteine lipid peroxidation

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Zinc alleviates the heat stress of primary cultured hepatocytes of broiler embryos via enhancing the antioxidant ability and attenuating the heat shock responses

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Animal Nutrition 2021年第3期7卷 621-630页

作者： Tingting Li Wengang He Xiudong Liao Xi Lin Liyang Zhang Lin Lu Yanli Guo zongping liu Xugang Luo Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009China Mineral Nutrition Research Division Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China College of Animal Science and Technology Gansu Agricultural UniversityLanzhou 730070China Department of Animal Science North Carolina State UniversityRaleighNC 27695USA College of Veterinary Medicine Yangzhou UniversityYangzhou 225009China

Zinc(Zn)has been shown to attenuate the adverse effects of heat stress on broilers,but the mechanisms involving this process remain unclear.We aimed to investigate possible protective mechanisms of Zn on primary cultured hepatocytes of broiler embryos subjected to heat stress.Three experiments were conducted.In Exp.1,hepatocytes were treated with 0,50,100,200,or 400μmol/L added Zn as inorganic Zn sulfate(iZn)for 12,24 or 48 h.In Exp.2,cells were exposed to 40℃(a normal temperature[NT])and 44℃(a high temperature[HT])for 1,2,4,6,or 8 h.In Exp.3,cells were preincubated with 0 or 50μmol/L Zn as iZn or organic Zn lysine chelate(oZn)for 8 h under NT,and then incubated with the same Zn treatments under NT or HT for 4 or 6 h.The biomarkers of antioxidative status and heat stress in cells were measured.The results in Exp.1 indicated that 50μmol/L Zn and 12 h incubation were the optimal conditions for increasing antioxidant ability of hepatocytes.In Exp.2,the 4 or 6 h incubation under HT was effective in inducing heat shock responses of hepatocytes.In Exp.3,HT elevated(P<0.01)malondialdehyde content and expressions of heat shock protein 70(HSP70)mRNA and protein,as well as HSP90 mRNA.However,Zn supplementation increased(P<0.05)copper zinc superoxide dismutase(CuZnSOD)activity and metallothionein mRNA expression,and effectively decreased(P<0.05)the ex-pressions of HSP70 mRNA and protein,as well as HSP90 mRNA.Furthermore,oZn was more effective(P<0.05)than iZn in enhancing CuZnSOD activity of hepatocytes under HT.It was concluded that Zn(especially oZn)could alleviate heat stress of broiler hepatocytes via enhancing their antioxidant ability and attenuating heat shock responses.

关键词： Broiler hepatocyte Zinc Heat stress Antioxidant ability Heat shock response

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Expression of VFA Receptors GPR41 and GPR43 in Cow Abomasums

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Agricultural Biotechnology 2013年第4期2卷 50-52页

作者： Haixia liu Jundong liu Chunyan SHAO zongping liu Jianji LI Jiangsu Agri-animal Husbandry Vocational College College of Veterinary Medicine Yangzhou University

With the enlargement of intensive feeding scale of dairy cows and improvement of milk yield, the incidence rate of displacement of abomasum in dairy cows increases gradually in recent years. Previous researches have shown that VFA in abomasums can significantly inhibit the shrinkage of smooth muscles of abomasums and lead to atony of abomasums, but the specific regulatory mechanisms are still unclear. In this study, the expression of VFA receptors GPR41 and GPR43 in cow abomasums were detected by PCR method, and the fragments were consistent with the excepted sizes. This study provided theoretical basis for the pathologic mechanism investigation, prevention and treatment of displacement of abomasum in dairy cows.

关键词： Dairy cow Smooth muscles of abomasums VFA receptor

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葛根素对镉致大鼠大脑皮质线粒体损伤的保护效应

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中国兽医科学 2024年第4期54卷 560-565页

作者：黄心昱徐明畅张文华邹辉顾建红刘学忠卞建春刘宗平袁燕扬州大学兽医学院江苏扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009

为了探究葛根素对镉(Cd)致大鼠大脑皮质线粒体损伤的保护效应,将24只SD大鼠随机分为对照组(Con)、葛根素组(Pur)、镉组(Cd)、镉与葛根素共处理组(Cd+Pur)。Pur组、Cd+Pur组大鼠每日以每千克体重200 mg葛根素(溶解于羧甲基纤维素钠)灌胃,... 详细信息

为了探究葛根素对镉(Cd)致大鼠大脑皮质线粒体损伤的保护效应,将24只SD大鼠随机分为对照组(Con)、葛根素组(Pur)、镉组(Cd)、镉与葛根素共处理组(Cd+Pur)。Pur组、Cd+Pur组大鼠每日以每千克体重200 mg葛根素(溶解于羧甲基纤维素钠)灌胃,Con组、Cd组大鼠每日用相同剂量羧甲基纤维素钠灌胃,持续7周。从第4周开始,Cd组、Cd+Pur组大鼠在灌胃的同时自由饮用75 mg/L镉水溶液,持续4周。试验结束后,剖杀采集大鼠大脑皮质,火焰原子吸收光谱法检测镉含量,化学发光法检测ATP水平,比色法检测NAD^(+)/NADH比率、Mn SOD活性和H_(2)O_(2)水平,实时荧光定量PCR法检测线粒体氧化应激相关基因(GST、CAT、Gpx、UCP1、UCP2和UCP3)转录水平。结果显示,与Con组相比,Cd组大鼠大脑皮质镉含量、H_(2)O_(2)水平、线粒体氧化应激相关基因转录水平显著上升(P0.05),MnSOD活性显著上升(P0.05)。结果表明,葛根素可降低镉暴露大鼠大脑皮质镉含量,同时葛根素通过缓解线粒体功能障碍、线粒体氧化应激减轻了镉致大鼠大脑皮质线粒体损伤。

关键词：镉葛根素大鼠大脑皮质线粒体损伤

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Construction and Identification of Mammary Gland-specific Expression Vector of Bovine Tracheal Antimicrobial Peptide (TAP)

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Agricultural Biotechnology 2013年第1期2卷 27-32页

作者： Suizhong CAO Xueping YAO Yafei CUI Deying YANG Kang YONG Shumin YU zongping liu College of Veterinary Medicine Sichuan Agricultural University College of Veterinary Medicine Yangzhou University

[ Objective] This study aimed to construct nmnmm_ry gland-specific expression vector of bovine tracheal antimicrobial peptide （TAP） gene. [ Method] TAP gene of dairy cattle was amplified from the mammary gland tissue by RT-PCR using a pair of primers which were designed according to bovine TAP cDNA se- quence （NM_174776） in GenBank, and then cloned into pMD19-T Simple vector for sequencing. The recombinant plasmid was digested using EcoRI and KpnI, the target gene fragment was recovered and inserted into general mammary gland-specific expression vector pBLG-EGFP harboring enhanced green fluorescent protein （ EGFP）, and transfected into bovine mammary epithelial cells （bMEC）, COS-7 cells and lactating rabbit mmmnary gland tissue by lipofectin transfection. The ex- pression of green fluorescent protein in transfected cells was detected under fluorescence microscopy, and the expression of TAP mRNA in rabbit mammary gland tis- sue was detected by semi-quantity RT-PCR. [ Result] The constructed mammary gland-specific expression vector pBLG-EGFP-TAP specifically expressed EGFP in transfected bMECs. In addition, semi-quantitative RT-PCR result showed that the expression level of TAP mRNA in rabbit mammary gland tissue was significantly enhanced after transfeeted with pBLG-EGFP-TAP. [ Conclusion] The mammary gland-specific expression vector pBLG-EGFP-TAP was successfully constructed, which provided important materials for further investigation of expression characteristics of TAP gene and prevention of bovine mastitis by using genetic engineering technology.

关键词： Dairy cattle Mastitis [3-defensin Trachea antimicrobial peptide （TAP） gene Manunary gland-specific expression vector

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Applying Dairy Cow Behavior in Management Practice

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Journal of Northeast Agricultural University(English Edition) 2009年第1期16卷 86-92页

作者： YUAN Kai liu zongping WANG Zongyuan College of Veterinary Medicine Yangzhou University Yangzhou 225009 China

Applying dairy cow behavior in management practice is an effective way of improving cow health, welfare and performance. This paper first reviewed daily time budget and normal patterns of dairy cow behavior, and then discussed the influence of major management conditions and practices （such as competitive environments, stocking density, grouping strategies） on cow＇s feeding, lying and social behavior. Finally, new findings of using feeding behavior to predict disorders in transition period were addressed. It was suggested that dairy researchers and farmers should take advantage of related knowledge of dairy cow behavior to improve dairy cow health and welfare. More research is required to further study dairy cow behavior so as to better apply it in practical management and meet the needs of production.

关键词： dairy cows behavior management

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不锈钢管螺旋筋海洋混凝土短柱轴压性能试验研究

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建筑结构学报 2024年第2期45卷 148-161页

作者：陈宗平刘丁瑗梁宇涵周济广西大学土木建筑工程学院广西南宁530004 南宁学院土木与建筑工程学院广西南宁530200 广西大学工程防灾与结构安全教育部重点实验室广西南宁530004

为研究不锈钢管螺旋筋海洋混凝土短柱的轴压性能,以螺旋筋直径与间距、纵筋直径与数量、螺旋筋与纵筋的截面配筋率之比为变化参数,设计了17组共51个轴压短柱进行静力加载试验,观察了短柱的受力破坏过程及形态,获取了轴向荷载-位移曲线... 详细信息

为研究不锈钢管螺旋筋海洋混凝土短柱的轴压性能,以螺旋筋直径与间距、纵筋直径与数量、螺旋筋与纵筋的截面配筋率之比为变化参数,设计了17组共51个轴压短柱进行静力加载试验,观察了短柱的受力破坏过程及形态,获取了轴向荷载-位移曲线及各钢材应变曲线,基于试验数据,分析各变化参数对该类短柱的承载力、轴压延性、耗能能力、损伤发展的影响规律。结果表明:短柱破坏形态均表现为不锈钢管局部屈曲压皱和混凝土斜向剪切破坏;配置螺旋筋和纵筋可以使短柱的承载力增大14.95%,延性系数增大73.25%,耗能系数增大19.69%,并抑制短柱的损伤发展;相同换算截面配筋率下,随螺旋筋间距增大(直径增大),短柱承载力、延性、耗能均呈递减趋势;随纵筋直径增大(数量减少),短柱承载力表现为先减小后增大、延性先增大后减小、耗能递减;螺旋筋与纵筋截面配筋率最优比值为0.875。最后提出了该类短柱的轴压承载力计算式,其计算值与试验值吻合良好。

关键词：海洋混凝土短柱不锈钢管螺旋筋轴压性能承载力计算

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镉对鸭破骨细胞焦亡的影响

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中国兽医学报 2022年第4期42卷 745-749页

作者：王怡马勇刚冉迪刘宗平扬州大学兽医学院江苏扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009

为探讨镉暴露对鸭破骨细胞焦亡的影响,本试验用不同浓度的镉(0,5和10μmol/L)处理鸭破骨细胞12 h。通过显微镜观察破骨细胞形态变化,ELISA试剂盒检测IL-1β和IL-18的含量,流式细胞术检测破骨细胞焦亡,Hoechst和PI染色观察细胞核数量变... 详细信息

为探讨镉暴露对鸭破骨细胞焦亡的影响,本试验用不同浓度的镉(0,5和10μmol/L)处理鸭破骨细胞12 h。通过显微镜观察破骨细胞形态变化,ELISA试剂盒检测IL-1β和IL-18的含量,流式细胞术检测破骨细胞焦亡,Hoechst和PI染色观察细胞核数量变化。结果显示,镉暴露后鸭破骨细胞表现出明显的形态改变,主要表现为细胞肿胀,细胞膜可见大量孔洞。与对照组相比,镉暴露明显增加了鸭破骨细胞内LDH的释放,细胞因子IL-1β和IL-18的产生也显著升高(P<0.01)。Caspase-1的活性较对照组显著增加(P<0.01),FITC和PI双染的阳性破骨细胞数也明显升高(P<0.01)。Hoechst和PI染色结果表明,镉暴露明显增加了红色和蓝色荧光强度。上述结果表明,镉暴露可诱导鸭破骨细胞发生焦亡。

关键词：镉破骨细胞焦亡鸭

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珊瑚海砂海水混凝土中CFRP-钢复合筋搭接性能

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复合材料学报 2024年第5期41卷 2645-2661页

作者：周济陈宗平徐炜圣覃伟恒刘丁瑗广西大学土木建筑工程学院南宁530004 南宁学院土木与建筑工程学院南宁530200 广西大学工程防灾与结构安全教育部重点实验室南宁530004

为研究珊瑚海砂海水混凝土(CSSC)中碳纤维增强复合材料(CFRP)-钢复合筋(简称“复合筋”)搭接连接受力性能,合理确定CSSC中复合筋的搭接长度,以复合筋直径、搭接长度、保护层厚度、混凝土强度、搭接净距、配箍率、混凝土及筋材种类为试... 详细信息

为研究珊瑚海砂海水混凝土(CSSC)中碳纤维增强复合材料(CFRP)-钢复合筋(简称“复合筋”)搭接连接受力性能,合理确定CSSC中复合筋的搭接长度,以复合筋直径、搭接长度、保护层厚度、混凝土强度、搭接净距、配箍率、混凝土及筋材种类为试验参数,对27组共81个搭接连接试件进行了对拉试验,获得了各试验参数对CSSC中复合筋搭接连接性能的影响规律。结果表明:当搭接长度小于17倍复合筋直径(d_(c))时,试件发生拔出-劈裂破坏,反之则发生拉断破坏。发生拔出-劈裂破坏时,复合筋表面出现严重的剪切损伤;当搭接长度小于14dc时,试件的搭接强度约为7.2 MPa,当搭接长度大于14d_(c)时,试件的搭接强度随搭接长度的增大逐渐减小;保护层厚度大于4d_(c)或CSSC轴心抗压强度大于30 MPa后,试件搭接强度的变化基本上可以忽略;增大配箍率,搭接强度逐渐增大,且劈裂裂缝的宽度显著减小;与钢筋试件相比,复合筋试件搭接强度的降幅超过25%。基于试验结果,建立了CSSC中复合筋搭接强度及搭接长度计算公式,计算结果与试验结果相符,并参考《混凝土结构设计规范》简化了搭接长度的计算公式。

关键词：珊瑚海砂海水混凝土 CFRP-钢复合筋搭接强度临界搭接长度简化公式

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枸杞酰胺体外对镉致成骨细胞凋亡的保护作用

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畜牧兽医学报 2023年第3期54卷 1273-1280页

作者：常颖艳赵鸿雁胡茂志刘宗平扬州大学生物科学与技术学院扬州225009 扬州大学兽医学院扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心扬州225009

旨在探究枸杞酰胺(aurantiamide acetate,AA)在镉(Cd)致成骨细胞凋亡中的保护效应,本研究以ROS1728大鼠成骨细胞系为研究对象,添加5μg·mL^(-1)AA预处理3 h后,加入20μg·mL^(-1)氯化镉(CdCl2)继续处理6 h。利用流式细胞术检测细胞凋... 详细信息

旨在探究枸杞酰胺(aurantiamide acetate,AA)在镉(Cd)致成骨细胞凋亡中的保护效应,本研究以ROS1728大鼠成骨细胞系为研究对象,添加5μg·mL^(-1)AA预处理3 h后,加入20μg·mL^(-1)氯化镉(CdCl2)继续处理6 h。利用流式细胞术检测细胞凋亡率、线粒体膜电位(mitochondrial membrane potential,MMP)、活性氧簇(reactive oxygen species,ROS),Western blot检测Bcl-2、Bax蛋白,高内涵细胞成像分析系统观察和分析细胞线粒体纹理结构和指数变化。结果显示,经过AA预处理后,较Cd处理组,AA极显著抑制Cd导致的ROS1728细胞凋亡率上升,Bax/Bcl-2比值增高,MMP下降,线粒体纹理结构指数下降,ROS含量增多(P<0.01)。上述结果表明,AA通过降低细胞内过氧化物含量,维持细胞线粒体结构的完整,抑制镉诱导的ROS1728细胞凋亡。

关键词：镉枸杞酰胺 ROS1728 凋亡线粒体 ROS

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