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Overexpression of SlOFP20 affects floral organ and pollen development

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Horticulture Research 2019年第1期6卷 154-169页

作者： Shengen Zhou zongli hu Fenfen Li Shibing Tian Zhiguo Zhu Anzhou Li Guoping Chen Laboratory of Molecular Biology of Tomato Bioengineering CollegeChongqing UniversityChongqingPeople’s Republic of China Institute of Vegetable Research Chongqing Academy of Agricultural SciencesChongqingPeople’s Republic of China

The OVATE gene was initially identified in tomato and serves as a key regulator of fruit *** are 31 OFP members in the tomato ***,their roles in tomato growth and reproductive development are largely ***,we cloned the OFP transcription factor *** plants overexpressing SlOFP20 displayed several phenotypic defects,including an altered floral architecture and fruit shape and reduced male ***20 overexpression altered the expression levels of some brassinosteroid(BR)-associated genes,implying that SlOFP20 may play a negative role in the BR response,similar to its ortholog OsOFP19 in ***,the transcript accumulation of gibberellin(GA)-related genes was significantly affected in the transgenic ***20 may play an important role in the crosstalk between BR and *** pollen germination assay suggested that the pollen germination rate of SlOFP20-OE plants was distinctly lower than that of WT *** addition,the tomato pollen-associated genes SlCRK1,SlPMEI,LePRK3,SlPRALF,and LAT52 were all suppressed in the transgenic *** data imply that SlOFP20 may affect floral organ and pollen development by modulating BR and GA signaling in tomato.

关键词： floral expressing pollen

Metabolic and molecular analysis of nonuniform anthocyanin pigmentation in tomato fruit under high light

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Horticulture Research 2019年第1期6卷 1065-1085页

作者： Yanjie Zhang Yan Li Wanping Li zongli hu Xiaohui Yu Yun Tu Min Zhang Jinyong huang Guoping Chen Bioengineering College Chongqing University400030 ChongqingPeople’s Republic of China School of Agricultural Sciences Zhengzhou University450001 ZhengzhouPeople’s Republic of China

Pigment intensity and patterns are important factors that determine the nutritional and market values of tomato *** acropetal manner of light-dependent anthocyanin accumulation with the highest levels at the stem end of the fruit makes Pro35S:BrTT8 tomato plants an ideal system for investigating the effects of light intensity on anthocyanin *** transcript analyses indicate that anthocyanin pigmentation in Pro35S:BrTT8 plants under high light might be coordinately regulated by the exogenous protein BrTT8 and endogenous proteins SlAN2 and ***,yeast two-hybrid assays showed that BrTT8 could interact efficiently with SlAN2,SlMYBL2,and ***,the physical interaction between BrTT8 and SlAN2 was validated by *** overexpression of SlAN2 and BrTT8 activated significant anthocyanin biosynthesis in infiltrated tobacco *** addition,the ability of SlMYBL2 to suppress anthocyanin accumulation was also demonstrated in infiltrated tobacco ***,these results prove that tissue-specific assemblage of the heterogeneous MYB-bHLH-WD40 complex consisting of SlAN2,BrTT8 and SlAN11 triggers nonuniform anthocyanin accumulation in tomato fruit under high ***,it is proposed that a negative-feedback loop fulfilled by SlMYBL2 also participates in the regulation of anthocyanin production.

关键词： light anthocyanin analysis

Spectral properties of LH2 exhibit very similar even when heterologously express LH2 with β-subunit fusion protein in Rhodobactersphaeroides

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Advances in Biological Chemistry 2013年第1期3卷 101-107页

作者： Zhiping Zhao Xin Nie zongli hu Guoping Chen Zaixin Li Zhi Zhang Bioengineering College Chongqing University Chongqing China School of Chemistry and Pharmaceutical Engineering Sichuan University of Science & Engineering Zigong China

Interactions between the light-harvesting subunits and the non-covalently bound photopigments attribute considerably to the spectral properties of photosynthetic bacteria light-harvesting complexes. In our previous studies, we have constructed a novel Rhodobacter sphaeroides expression system. In the present study, we focus on the spectral properties of LH2 when heterologously express LH2 with β-subunit- GFP fusion protein in Rb. sphaeroides. Near infra-red spectrum of LH2 remained nearly unchanged as measured by spectroscopy. Fluorescence spectrum suggested that the LH2 with β-subunit-GFP fusion protein complexes still possessed normal activity in energy transfer. However, photopigments contents were significantly decreased to a very low level in the LH2 with β-subunit-GFP fusion protein complexes compared to that of LH2. FT-IR spectra indicated that interactions between photopigments and LH2 α/β- subunits appeared not to be changed. It was concluded that the LH2 spectral properties exhibited very similar even when heterologously expressed LH2 b-subunit fusion protein in Rb. sphaeroides. Our present study may supply a new insight into better understand the interactions between light-harvesting subunits and photopigments and bacterial photosynthesis and promote the development of the novel Rb. sphaeroides expression system.

关键词： LH2 Spectral Property FT-IR Photopigment

The transgenic potato bearing Tm-22 from tomato exhibited high activity against TMV and ToMV

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The transgenic potato bearing Tm-22 from tomato exhibited hi...

重庆市生物化学与分子生物学学术会议

作者： Jiange Gao,zongli hu,Guoping Chen (Bioengineering College,Chongqing University,Chongqing,400044)

Solanum tuberosum,is the world’s fourth major food *** has been reported that cloned resistance genes could be transformed into some species to achieve resistance against *** order to identify whether the Tm-2 resist... 详细信息

关键词： Tm-2~2 gene potato virus-resistance

CASIASS-1000眼前节OCT对初发期和膨胀期皮质性白内障患者晶状体厚度及前房结构测量的比较

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中华实验眼科杂志 2017年第12期35卷 1092-1096页

作者：胡宗莉叶剑陆军军医大学大坪医院眼科重庆400042

背景随着病情发展，皮质性白内障患眼的晶状体厚度及前房结构将发生变化，可能对眼部结构及功能产生重要影响，需要一种可直接测量晶状体前后表面及房角结构的检查方法，以量化不同分期白内障的上述指标变化。目的采用CASIASS-1000眼前... 详细信息

背景随着病情发展，皮质性白内障患眼的晶状体厚度及前房结构将发生变化，可能对眼部结构及功能产生重要影响，需要一种可直接测量晶状体前后表面及房角结构的检查方法，以量化不同分期白内障的上述指标变化。目的采用CASIASS-1000眼前节光相干断层扫描（AS-OCT）测量并比较初发期和膨胀期白内障患者晶状体厚度及房角相关指标。方法采用观察性研究设计。连续纳入2015年10月16-30日于陆军军医大学大坪医院眼科门诊就诊的初发期和膨胀期皮质性白内障患者50例87眼，其中男21例，女29例；初发期白内障患者28例44眼，平均年龄（56．46±12．90）岁，膨胀期白内障患者22例43眼，平均年龄（70．95±8．81）岁。利用CASIASS-1000AS-OCT测量患眼晶状体厚度、房角开放距离（AOD）、前房角隐窝面积（ARA）、小梁网虹膜间面积（TISA）、小梁网虹膜角（TIA）及前房深度（ACD），比较2个组患眼相关指标的差异。结果初发期白内障晶状体平均厚度为（4．438±0．487）mm，膨胀期白内障晶状体厚度平均为（5．094±0．451）mm，膨胀期白内障患眼晶状体明显增厚，平均增加（0．656＋0．101）mm，差异有统计学意义（t=-6．513，P=0．000）；初发期白内障患眼ACD平均为（2．966＋0．326）mm，膨胀期患眼ACD平均为（2．534±0．376）mm，随着白内障发展，ACD平均减少（0．432＋0．075）mm，差异有统计学意义（t=5．730，P=0．000）。在房角相关测量指标中，膨胀期白内障患眼AOD500、AOD750、ARA500、ARA750、TISA500、TISA750、TIA500和TIA750明显低于初发期白内障患眼，差异均有统计学意义（均P=0．000）。结论膨胀期白内障患者晶状体厚度明显增加，前房变浅，房角明显变窄。CASIASS-1000AS-OCT测量房角扫描时间短，清晰度高，具有很好的临床应用前景。

关键词：眼前节光相干断层扫描皮质性白内障晶状体厚度前房

Expression of OsLEA3 gene enhanced the tolerance to abiotic stresses in *** and Rice

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Expression of OsLEA3 gene enhanced the tolerance to abiotic ...

重庆市生物化学与分子生物学学术会议

作者： Tingzhang hu,Xiaoxiao Qu,zongli hu,Shuai He,Guoping Chen (Bioengineering College,Chongqing University,Chongqing 400044)

The OsLEA3 is a late embryogenesis abundant group 3 *** OsLEA3 gene was cloned from rice seeding which was treated by drought and constructed into pET30a vector,and then transformed into Escherichia coli strain ***-PAGE analysis showed that a 30 KDa protein band was expressed in OsLEA3 transgenic strain induced by 1 mM *** growth rates of OsLEA3 transgenic strain and

关键词： rice LEA3 gene Escherichia coli resistant

Molecular cloning and characterization of FLOWERING LOCUS C(FLC)from Brassica campestris *** Bailey(Purple Flowering Stalk)

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Molecular cloning and characterization of FLOWERING LOCUS C(...

重庆市生物化学与分子生物学学术会议

作者： Gongling hu,zongli hu,Yong Li,Feng Gu,Guoping Chen (Bioengineenng College,Chongqing University,Chongqing 400044)

FLOWERING LOCUS C(FLC) which encodes a MAD-S domain protein is a flowering repressor and in the pivotal location of Arabidopsis flowering *** Brassica species,there are several FLC homologues involved in flowering time like Arabidopsis *** the analysis of FLC homologues expression and variation in FLC1 associated with early flowering phenotype in Purple Flowering Stalk

关键词： Purple Flowering Stalk FLOWERING LOCUS C 1 alternative splicing variation

有晶状体眼后房型人工晶状体植入矫正屈光不正的临床进展

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中华眼视光学与视觉科学杂志 2019年第9期21卷 715-720页

作者：胡宗莉叶剑中国人民解放军陆军军医大学大坪医院眼科重庆400042

后房型人工晶状体又称可植入式隐形眼镜(ICL),它为屈光不正的治疗提供了角膜手术以外的新途径,可应用于高度近视、屈光参差、圆锥角膜等患者。目前临床最常用的是V4c型ICL,其特点是带有中央孔,可改善房水的引流并增加晶状体前表面的房... 详细信息

后房型人工晶状体又称可植入式隐形眼镜(ICL),它为屈光不正的治疗提供了角膜手术以外的新途径,可应用于高度近视、屈光参差、圆锥角膜等患者。目前临床最常用的是V4c型ICL,其特点是带有中央孔,可改善房水的引流并增加晶状体前表面的房水循环,且术前或术中不再需要行周边虹膜切除术。ICL植入术可有效提高裸眼及矫正视力,且术后眼压长期稳定。V4c型ICL植入术后白内障发生率较传统V4型更低,其长期安全性及有效性高,在目前的临床应用中取得了良好的疗效,可作为矫正上述屈光不正的主要方法。随着ICL的临床应用与研究进展,有关ICL植入术的疗效及并发症的讨论越来越多,明确手术的安全有效性,以及对术后并发症的评估和处理是临床医师面临的重要问题。现就ICL植入术的临床研究进展进行综述。

关键词：后房型人工晶状体屈光不正临床疗效并发症

A dynamic analysis system for Cisco IOS based on virtualization

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A dynamic analysis system for Cisco IOS based on virtualizat...

第三届多媒体信息网络与安全国际会议

作者： Yuxiang HAN Shengli LIU Xiaoyan SU zongli hu Zhengzhou Institute of Information Science and Technology Zhengzhou China

It is difficult to analyze and debug Cisco IOS with traditional debugging and disassembler tools such as GDB and IDA pro. These tools can’t debug Cisco IOS in single step mode. This paper describes a dynamic analysis system based on a modification hardware emulator called Dynamips. Then, a new solution of inserting breakpoint with network events will be provided to analyze network communication procedures.

关键词： IOS virtualization emulator dynamic debugging system breakpoint