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Identif ication of a hypersensitive response core peptide of HrpZ and its role in increasing grape downy mildew resistance

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Horticultural Plant Journal 2024年第5期10卷 1112-1120页

作者： zongbao Fan Xueqiang Guan Zhichang Zhang Yushuai sun Fei Wang Huiru Chi Yuxin Yao State Key Laboratory of Crop Biology Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China Shandong Academy of Grape/Shandong Technology Innovation Center of Wine Grape and Wine JinanShandong 250100China Zhichang Agricultural Science and Technology Development Co. Ltd.RizhaoShandong 276511China

Harpins play a key role in inducing disease resistance in crops,and identifying their core functional regions and establishing a system for their efficient expression would be very *** this study,large amounts of soluble fusion proteins of harpin HrpZ and its subpeptides were obtained via the optimized induction conditions(28℃ with 0.5 mmol·L^(-1) IPTG for 6 h)in Escherichia coli BL21(DE3).Hypersensitive response(HR)assays demonstrated that the C-terminal 66 aa of HrpZ(HrpZ_C_2_2)elicited a strong HR in tobacco(Nicotiana benthamiana)and grape(Flame Seedless)***,treatment with HrpZ,and particularly HrpZ_C_2_2,significantly reduced the disease incidence and severity index of field vine leaves and those inoculated with downy *** determination of the physiological parameters indicated that HrpZ,and especially HrpZ_C_2_2,improved the photosynthesis-and chlorophyll fluorescence-related parameters,enhanced the activity of defense-related enzymes,including SOD,POD,CAT and PAL,and increased the H_(2)O_(2) ***,we efficiently expressed a core peptide of HrpZ and elucidated its strong ability to elicit a HR and resistance to downy *** research provides insight into understanding the structure and function of HrpZ and will advance the application of HrpZ_C_2_2 to increase the resistance of grapevine to downy mildew.

关键词： Grapevine Harpin HrpZ Prokaryotic expression Downy mildew Hypersensitive response

An improved YOLOv7 for the state identification of sliding chairs in railway turnout

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High-Speed Railway 2024年第2期2卷 71-76页

作者： Yuan Cao zongbao Liu Feng Wang Shuai Su Yongkui sun Wenkun Wang National Engineering Research Center of Rail Transportation Operation and Control System Beijing Jiaotong UniversityBeijing 100044China School of Automation and Intelligence Beijing Jiaotong UniversityBeijing 100044China Frontiers Science Center for Smart High-speed Railway System Beijing Jiaotong UniversityBeijing 100044China Zhuzhou CRRC Times Electric Co. LTD.Zhuzhou 412001China

The sliding chairs are important components that support the switch rail conversion in the railway *** to the harsh environmental erosion and the attack from the wheel vibration,the failure rate of the sliding chairs accounts for up to 10%of the total failure number in ***,there is little research carried out in the existing literature to diagnose the deterioration states of the sliding *** fill out this gap,by utilizing the images containing the sliding chairs,we propose an improved You Only Look Once version 7(YOLOv7)to identify the state of the sliding ***,to meet the challenge brought by the small inter-class differences among the sliding chair states,we first integrate the Convolutional Block Attention Module(CBAM)into the YOLOv7 backbone to screen the information conducive to state ***,an extra detector for a small object is customized into the YOLOv7 network in order to detect the small-scale sliding chairs in ***,we revise the localization loss in the objective function as the Efficient Intersection over Union(EIoU)to optimize the design of the aspect ratio,which helps the localization of the sliding ***,to address the issue caused by the varying scales of the sliding chairs,we employ K-means++to optimize the priori selection of the initial anchor ***,based on the images collected from real-world turnouts,the proposed method is verified and the results show that our method outperforms the basic YOLOv7 in the state identification of the sliding chairs with 4%improvements in terms of both mean Average Precision@0.5(mAP@0.5)and F1-score.

关键词： Railway turnout Sliding chairs State identification Object detection

利用响应面法优化HS-SPME镇江香醋香气成分的条件

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中国酿造 2009年第8期28卷 45-48页

作者：李国栋孙宗保江苏大学食品与生物工程学院江苏镇江212013

为了优化镇江香醋香气成分的提取,在单因素实验的基础上,根据Box-Benhnken的中心组合实验设计原理,采用3因素3水平的响应面分析法,以提取物质的质(色)谱图总峰面积为响应值做响应面和等高线图。结果表明,HS-SPME萃取镇江香醋香气成分的... 详细信息

为了优化镇江香醋香气成分的提取,在单因素实验的基础上,根据Box-Benhnken的中心组合实验设计原理,采用3因素3水平的响应面分析法,以提取物质的质(色)谱图总峰面积为响应值做响应面和等高线图。结果表明,HS-SPME萃取镇江香醋香气成分的最佳条件是氯化钠添加量2.5 g,萃取温度50.16℃,萃取时间39.76 min。

关键词：镇江香醋顶空固相微萃取香气成分响应面分析中心组合设计

Molecularly engineered lignin to polyphenol via organocatalysis as an active sunscreen ingredient

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Journal of Bioresources and Bioproducts 2024年第2期9卷 197-210页

作者： Yuanlong Guo Pengcheng Liu Lei Deng Changwei Lui Michael North Gang Hu Qitian Huang zongbao Kent Zhao Haibo Xie Department of Polymeric Materials&Engineering Guizhou UniversityGuiyang 550025China KZJ New Materials Group Guizhou Co.Ltd. Guizhou 551200China Green Chemistry Centre of Excellence Department of ChemistryUniversity of YorkYork YO105DDUK Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023China

Phenolation is one of the effective strategies to synthesize lignin-based polyphenols,improve lignin’s properties,and extend its value-added applications in biological,medicinal and cosmetic ***,by taking the structural feature advantage of lignin,an effective and green strategy was developed to molecularly engineer lignin into a robust lignin-3-(2-hydroxyphenyl)propionate ester(LPPE)derivative via a transesterification reaction between 3,4-dihydrocoumarin(DHC)and the aliphatic hydroxyls in lignin under *** strategy is optimized and the novel derivative was systematically characterized by ^(1)H,^(13)C and ^(31)P nuclear magnetic resonance(NMR)and Fourier transform infrared(FT-IR)*** findings indicated that the successful introduction of 3-(2-hydroxyphenyl)propionate groups using a OH groups/DHC/organic base mo-lar ratio of 1꞉1꞉0.3 at 120℃ for 6 h increased the content of phenolic hydroxyl groups from 1.7931 to 3.0179 mmol/g,and the LPPE exhibited excellent ultraviolet-absorbing and antioxidant per-formance with up to 90%free radical scavenging activity within 20 min using 5 mg/mL of *** addition,good biocompatibility and a high sun protection factor(SPF)value of 40.9 were achieved at 5%(w)dosage of LPPE in the cream,indicating its significant application potential in sunscreen.

关键词： Phenolation of lignin Organocatalysis 3,4-dihydrocoumarin Antioxidant sunscreen cream

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基于嗅觉可视化的鲳鱼TVB-N预测模型

江苏大学学报（自然科学版） 2013年第1期34卷 38-43页

作者：黄星奕蒋飞燕穆丽君孙宗保江苏大学食品与生物工程学院江苏镇江212013

采用半微量凯氏定氮法对4℃恒温下储藏的冰鲜鲳鱼的TVB-N进行检测,同时利用嗅觉可视化技术提取其挥发性气味信息,将嗅觉可视化传感器阵列与鲳鱼挥发性气体发生反应前后的RGB颜色变化值作为传感器响应值,分别建立TVB-N的偏最小二乘法(PLS... 详细信息

采用半微量凯氏定氮法对4℃恒温下储藏的冰鲜鲳鱼的TVB-N进行检测,同时利用嗅觉可视化技术提取其挥发性气味信息,将嗅觉可视化传感器阵列与鲳鱼挥发性气体发生反应前后的RGB颜色变化值作为传感器响应值,分别建立TVB-N的偏最小二乘法(PLS)、遗传偏最小二乘法(GA-PLS)预测模型.经比较,GA-PLS模型预测效果更优,预测集相关系数达到0.851 7;可视化传感器阵列响应信号与TVB-N之间存在较高的相关性,可以快速预测出鱼储藏期间TVB-N变化,从而无损评价鱼的新鲜度.

关键词：鲳鱼嗅觉可视化检测挥发性盐基氮遗传-偏最小二乘法

HS-SPME/GC-MS/GC-O对镇江香醋特征香气成分的确定

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江苏大学学报（自然科学版） 2010年第2期31卷 139-144页

作者：孙宗保赵杰文邹小波李国栋江苏大学食品与生物工程学院江苏镇江212013 江苏恒顺集团有限公司江苏镇江212000

采用顶空固相微萃取提取了镇江香醋的挥发性成分,用气相色谱-质谱联用技术结合气相色谱-嗅觉测量法以及化合物的保留指数,鉴定了镇江香醋的主要香气成分;同时利用气相色谱-嗅觉测量技术,采用探测频率和探测强度相结合的办法,初步确定了... 详细信息

采用顶空固相微萃取提取了镇江香醋的挥发性成分,用气相色谱-质谱联用技术结合气相色谱-嗅觉测量法以及化合物的保留指数,鉴定了镇江香醋的主要香气成分;同时利用气相色谱-嗅觉测量技术,采用探测频率和探测强度相结合的办法,初步确定了乙酸、3-甲基丁酸、乙酸乙酯、乙酸-3-甲基-丁酯、乙酸苯乙酯、苯乙醇、2,3-丁二酮、二氢-5-戊基-2(3H)-呋喃酮、2-甲基丙醛、3-甲基丁醛、糠醛、苯甲醛、三甲基恶唑、2,3-二甲基吡嗪、三甲基吡嗪、四甲基吡嗪16种化合物为镇江香醋的特征香味物质.

关键词：镇江香醋气相色谱-质谱联用气相色谱-嗅觉测量特征香味物质

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图像处理技术在食品粒度检测中的应用研究

食品研究与开发 2005年第6期26卷 164-166页

作者：孙宗保蔡健荣姜松江苏大学生物与环境工程学院镇江212013

简要阐述图像处理技术检测食品粉体物料粒度的基本原理和处理过程,并对检测的结果进行分析,结果表明运用图像处理技术进行粒度检测,测定分布状态直观明了,检测速度快,结果稳定可靠。

关键词：显微图像粒度图像处理测量

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基于超声成像技术的冷鲜与解冻牛肉鉴别方法

农业机械学报 2019年第7期50卷 349-354,166页

作者：孙宗保王天真邹小波闫晓静梁黎明刘小裕江苏大学食品与生物工程学院

针对市场上存在以解冻牛肉冒充冷鲜牛肉售卖的问题,研究了利用超声成像技术对冷鲜与解冻牛肉进行质构分析与鉴别的可行性。首先,通过超声成像设备采集冷鲜与解冻牛肉超声图像信息,并结合质构参数、微观结构和部分理化指标分析图像差异... 详细信息

针对市场上存在以解冻牛肉冒充冷鲜牛肉售卖的问题,研究了利用超声成像技术对冷鲜与解冻牛肉进行质构分析与鉴别的可行性。首先,通过超声成像设备采集冷鲜与解冻牛肉超声图像信息,并结合质构参数、微观结构和部分理化指标分析图像差异成因。然后,采用灰度共生矩阵法提取图像纹理特征值,利用主成分分析法进行数据降维处理,分别建立线性判别分析(Linear discriminant analysis,LDA)和支持向量机(Support vector machine,SVM)两种判别模型。结果表明,两种判别模型均有较好的分类效果,其中LDA模型更优,当主成分数为5时,模型的训练集和测试集识别率均达到100%。说明应用超声成像技术对冷鲜与解冻牛肉进行质构分析与鉴别是可行的。

关键词：冷鲜牛肉解冻牛肉超声成像判别模型

基于高光谱和超声成像技术的原切与合成调理牛排鉴别

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食品科学 2021年第8期42卷 257-263页

作者：孙宗保王天真邹小波刘源梁黎明李君奎刘小裕江苏大学食品与生物工程学院江苏镇江212013 镇江市食品药品监督检验中心江苏镇江212000

针对市场上存在合成调理牛排冒充原切售卖的现象,研究利用高光谱和超声成像技术对它们进行鉴别的方法。分别采集原切与合成调理牛排的高光谱和超声图像信息,利用灰度共生矩阵法提取图像的纹理特征值,分别建立线性判别分析、K最邻近(K-ne... 详细信息

针对市场上存在合成调理牛排冒充原切售卖的现象,研究利用高光谱和超声成像技术对它们进行鉴别的方法。分别采集原切与合成调理牛排的高光谱和超声图像信息,利用灰度共生矩阵法提取图像的纹理特征值,分别建立线性判别分析、K最邻近(K-nearest neighbor,KNN)、反向传播人工神经网络和极限学习机(extreme learning machine,ELM)4种鉴别模型,而后将2种技术数据融合建模,并采用连续投影法、竞争性自适应重加权算法(competitive adaptive reweighted sampling,CARS)、变量组合集群分析(variables combination population analysis,VCPA)法3种方法筛选特征变量建模。结果表明:合成调理牛排的肉块组织均匀,超声图像信号弱、均一性好,与原切调理牛排图像存在差异。高光谱和超声成像技术的最佳模型分别为KNN和ELM,模型预测集识别率分别为95.00%和90.00%。数据融合后建模,最佳模型ELM模型预测集识别率模型为97.50%,在3种变量选择方法中,CARS和VCPA选择的纹理变量建立的模型预测集识别率达到100.00%。研究表明高光谱和超声成像数据融合结合变量选择方法可以快速准确地鉴别原切和合成调理牛排。

关键词：超声成像技术高光谱成像技术调理牛排数据融合