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Cancer Biology & Medicine 2013年第2期10卷 117-120页

作者： Yi-Hao Wang Rong Fu zong-hong shao Department of Hematology Tianjin Medical University General Hospital

A 60-year-old woman with squamous cell carcinoma in the right lung was successfully treated with four cycles of combination chemotherapy after surgery, and complete remission was achieved. However, the patient developed myelodysplastic syndrome (MDS) RAEB-2 with myelofibrosis after remission, possibly because of chemotherapy or DNA methylation. The patient responded well to dacitabine (Dacogen), suggesting that DNA hypomethylation agents can be a promising therapy to retard the progression of a second tumor or carcinoma.

关键词： DNA methylation myelodysplastic syndrome myelofibrosis squamous cell lung cancer

ROLE OF B LYMPHOCYTE AND ITS SUBPOPULATIONS IN PATHOGENESIS OF IMMUNORELATED PANCYTOPENIA

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Chinese Medical Sciences Journal 2007年第3期22卷 199-202页

作者： Rong Fu zong-hong shao hong Liu Yu-hong Wu Hua-quan Wang Li-min Xing Department of Hematology and Oncology General Hospital of Tianjin Medical University Tianjin 300052

Objective To measure the quantities and apoptosis-related protein levels of B lymphocyte in the patients with immunorelated pancytopenia （IRP） and explore the action of B lymphocyte in the pathogenic mechanism of IRP. Methods Quantifies of whole B lymphocytes and CD5^＋ B lymphocytes as well as the expressions of Fas and Bcl-2 in B lymphocytes in 35 patients with untreated IRP, 15 IRP patients in complete remission （CR）, and 10 normal controls were assayed by flow cytometry. The percentages of B lymphocyte and CD5^＋ B lymphocyte were significantly higher in untreated IRP patients than in CR IRP patients and normal controls （ P 〈 0. 05 ）, and there was no significant difference between the latter two groups （ P 〉 0. 05 ）. There was no significant difference of Fas expression in B lymphocyte among three groups （ P 〉 0. 05）. The expression of Bcl-2 in B lymphocyte was significantly higher in untreated patients than in CR patients or normal controls （ P 〈 0. 01 ）, and significantly higher in CR patients than in normal controls （ P 〈 0. 01 ）. The apoptosis. related index was significantly lower in untreated patients than in CR patients or normal controls （ P 〈 0. 05 ）, and signif. icantly lower in CR patients than in normal controls （ P 〈 0. 05 ）. The percentage of B lymphocyte was positively correlated with post-treated response time （ r = 0. 53, P 〈 0. 01 ）. Conclusion The production of auto-antibodies in IRP patients probably has some relationship with the abnormal quantifies of B lymphocyte and its subpopulations as well as with the inhibition of B lymphocyte apoptosis.

关键词： immunorelated pancytopenia B lymphocyte quantifies apoptosis

Advances in the role of helper T cells in autoimmune diseases

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Chinese Medical Journal 2020年第8期133卷 968-974页

作者： Xiao-Mei Zhang Chun-Yan Liu zong-hong shao Department of Hematology Tianjin Medical University General HospitalTianjin 300052China

Autoimmune diseases are primary immune diseases in which autoreactive antibodies or sensitized lymphocytes destroy and damage tissue and cellular components, resulting in tissue damage and organ dysfunction. Helper T cells may be involved in the pathogenesis of autoimmune diseases under certain conditions. This review summarizes recent research on the role of helper T cells in autoimmune diseases from two aspects, helper T cell-mediated production of autoantibodies by B cells and helper T cell-induced activation of abnormal lymphocytes, and provides ideas for the treatment of autoimmune diseases. The abnormal expression of helper T cells promotes the differentiation of B cells that produce autoantibodies, which leads to the development of different diseases. Among them, abnormal expression of Th2 cells and T follicular helper cells is more likely to cause antibody-mediated autoimmune diseases. In addition, abnormal activation of helper T cells also mediates autoimmune diseases through the production of abnormal cytokines and chemokines. Helper T cells play an essential role in the pathogenesis of autoimmune diseases, and a full understanding of their role in autoimmune diseases is helpful for providing ideas for the treatment of autoimmune diseases.

关键词： Helper T cells B cells Autoimmune disease

PRAME Gene Expression in Acute Leukemia and Its Clinical Significance

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Clinical oncology and cancer researeh 2012年第1期9卷 73-76页

作者： Kai Ding Xiao-ming Wang Rong Fu Er-bao Ruan Hui Liu zong-hong shao Department of Hematology General Hospital of Tianjin Medical University

Objective To investigate the expression of the preferentially expressed antigen of melanoma （PRAME） gene in acute leukemia and its clinical significance. Methods The level of expressed PRAME mRNA in bone marrow mononuclear cells from 34 patients with acute leukemia （AL） and in 12 bone marrow samples from healthy volunteers was measured via RT-PCR. Correlation analyses between PRAME gene expression and the clinical characteristics （gender, age, white blood count, immunophenotype of leukemia, percentage of blast cells, and karyotype） of the patients were performed. Results The PRAME gene was expressed in 38.2% of all 34 patients, in 40.7% of the patients with acute myelogenous leukemia （AML, n=27）, and in 28.6% of the patients with acute lymphoblastic leukemia （ALL, n=7）, but was not expressed in the healthy volunteers. The difference in the expression levels between AML and ALL patients was statistically significant. The rate of gene expression was 80% in M~, 33.3% in M2, and 28.6% in M~. Gene expression was also found to be correlated with CDl5 and CD33 expression and abnormal karyotype, but not with age, gender; white blood count or percentage of blast cells. Conclusions The PRAME gene is highly expressed in acute leukemia and could be a useful marker to monitor minimal residual disease. This gene is also a candidate target for the immunotherapy of acute leukemia.

关键词： preferentially expressed antigen of melanoma gene acute leukemia minimal residual disease immunotherapy

SUBTYPES OF B LYMPHOCYTES IN PATIENTS WITH AUTOIMMUNE HEMOCYTOPENIA

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Chinese Medical Sciences Journal 2007年第2期22卷 128-131页

作者： Li-min Xing zong-hong shao Rong Fu hong Liu Jun Shi Jie Bai Mei-feng Tu Hua-quan Wang Zhen-zhu Cui Hai-rong Jia Juan Sun Chong-li Yang Department of Hematology and Oncology General Hospital of Tianjin Medical University Tianjin 300052 Department of Hematology Institute of Hematology and Blood Diseases Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Tianjin 300020

Objective To investigate the quantities of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia and the relationship between quantities of CD5+ B lymphocytes and clinical or laboratorial parameters. Methods Quantities of CD5+ B lymphocytes in the bone marrow of 14 patients with autoimmune hemolytic anemia (AIHA) or Evans syndrome, 22 immunorelated pancytopenia (IRP) patients, and 10 normal controls were assayed by flow cytometry. The correlation between their clinical or laboratorial parameters and CD5+ B lymphocytes was analyzed. Results The quantity of CD5+ B lymphocytes of AIHA/Evans syndrome (34.64%±19.81%) or IRP patients (35.81%±16.83%) was significantly higher than that of normal controls (12.00%±1.97%, P0.05). In all hemocytopenic patients, the quantity of bone marrow CD5+ B lymphocytes showed significantly negative correlation with serum complement C3 level (r=-0.416, P0.05) or colony forming unit-granulocyte-macrophage colonies (r=-0.214, P>0.05). Conclusions The quantity of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia significantly increases and is correlated with disease severity and clinical response, which suggest that CD5+ B lymphocytes

关键词： autoimmune hemocytopenia B lymphocyte subtype

Expression of DLK1 Gene in the Bone Marrow Cells of Patients with Myelodysplastic Syndromes and Its Clinical Significance

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Clinical oncology and cancer researeh 2012年第3期9卷 188-191页

作者： Lan-zhu Yue Rong Fu Hua-quan Wang Li-juan Li Er-bao Ruan Guo-jin Wang Wen Qu Yong Liang Jing Guan Yu-hong Wu hong Liu Jia Song Xiao-ming Wang Li-min Xing zong-hong shao Department of Hematology General Hospital of Tianjin Medical University

Objective This study aims to investigate the expression of delta-like 1 （DLK1） gene in the bone marrow cells of patients with myelodysplastic syndromes （MDS） and to explore its molecular characteristics for the early diagnosis of MDS. Methods The expression of DLK1 mRNA in the bone marrow cells of cases with MDS, acute myeloid leukemia （AML）, and normal control groups were measured by real-time polymerase chain reaction and were analyzed for clinical significance. Results Significantly higher expression of DLK1 mRNA was observed in the bone marrow cells of MDS patients （0.7342±0.3652） compared with the normal control group （0.4801±0.1759）（P〈0.05）. The expression of DLK1 mRNA had a positive correlation with the proportion of bone marrow blasts （r=0.467, P〈0.05）. Moreover, DLK1 mRNA expression was significantly increased as MDS progressed （P〈0.05）. Patients with abnormal karyotypes exhibited significantly higher expression of DLK1 mRNA （0.9007±0.4334） than those with normal karyotypes （0.6411±0.2630）（P〈0.05）. Subsequently, patients with highly expressed DLK1 （≥0.8） presented significantly higher malignant clone burden （0.4134±0.3999） than those with lower DLK1 expression （〈0.8）,（0.1517±0.3109）, （P〈0.05）. Conclusions The DLK1 gene was highly expressed in MDS patients, and was increased as MDS progressed. The expression of DLK1 mRNA was positively correlated with the proportion of the bone marrow blasts. A high expression of DLK1 gene suggested a higher malignant clone burden of MDS.

关键词： DLK1 gene myelodysplastic syndromes expression

BURDEN OF ABNORMAL HEMATOPOIETIC CLONE IN PATIENTS WITH MYELODYSPLASTIC SYNDROMES

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Chinese Medical Sciences Journal 2006年第2期21卷 99-103页

作者： Hua-quan Wang zong-hong shao Jun Shi Yan-ran Cao hong Liu Jie Bai Mei-feng Tu Li-ming Xing Zhen-zhu Cui Shi-he Liu Jun Sun Hai-rong Jia Tian-ying Yang the First Department of Hematology Institute of Hematology ＆ Blood Diseases HospitalChinese Academy of Medical Sciences ＆ Peking Union Medical College Tianjin 300020 Department of Hematology and Oncology General Hospital Tianjin Medical University Tianjin 300052

Objective To investigate the role of the burden of abnormal hematopoietic clone in the development of myelodysplastic syndromes (MDS). Methods The ratio of the bone marrow cells with abnormal chromosomes to the total counted bone marrow cells was regarded as the index of MDS clone burden. The disease severity related parameters including white blood cell count, hemoglobin, platelet count, lactate dehydrogenase level, bone marrow blast, myeloid differentiation index, micromegakaryocyte, transfusion, interleukin-2, tumor necrosis factor (TNF), CD4^+ and CD8^+ T cells of MDS patients were assayed, and the correlations between those parameters and MDS clone burden were also analyzed. Results The clone burden of MDS patients was 67.4%±36.2%. MDS clone burden positively correlated with bone marrow blasts (r = 0.483, P50%) and low clone burden (≤50%) groups were 7.78%±5.51% and 3.45%±3.34%, 56.06±14.28 g/L and 76.40±24.44 g/L, (1.82±0.48)×10~ 12 /L and (2.32±0.66)×10~ 12 /L, respectively (all P<0.05). CD4^+ T lymphocytes of MDS patients and normal controls were (0.274±0.719)×10~ 9 /L and (0.455±0.206)×10~ 9 /L, respectively (P<0.05). CD8^+ T lymphocytes of MDS patients and normal controls were (0.240±0.150)×10~ 9 /L and (0.305±0.145)×10~ 9 /L, respectively. The serum level of interleukin-2 of MDS patients (6.29±3.58 ng/mL) was significantly higher than normal control (3.11±1.40 ng/mL, P<0.05). The serum level of TNF of MDS patients and normal control group were 2.42±1.79 ng/mL and 1.68±0.69 ng/mL, respectively. The ratio of CD4 to CD8 was higher in high clone burden MDS patients (1.90±0.52) than that in low clone burden patients (0.97±0.44, P<0.05). Conclusion The quantitive clonal karyotype abnormalities and deficient T cell immunity are important parameters for evaluating MDS severity and predicting its progression.

关键词： myelodysplastic syndromes karyotype T lymphocyte

TET2 Expression in Bone Marrow Mononuclear Cells of Patients with Myelodysplastic Syndromes and Its Clinical Significances

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Clinical oncology and cancer researeh 2012年第1期9卷 34-37页

作者： Wei Zhang zong-hong shao Rong Fu Hua-quan Wang Li-juan Li Jun Wang Wen Qu Yong Liang Guo-jin Wang Xiao-ming Wang Yuhong Wu hong Liu Jia Song Jing Guan Li-min Xing Department of Hematology Tianjin Medical University General Hospital

Objective To investigate the expression of TET2 mRNA and protein in the bone marrow mononuclear cells （BMMNC） of patients with myelodysplastic syndrome （MDS） and its clinical significance. Methods The expression of TET2 mRNA and protein in bone marrow mononuclear cells （BMMNC） of 32 patients with MDS and 20 healthy donors was examined by qPCR and Western blot. Results The expression of TET2 mRNA in BMMNC was down-regulated in MDS patients compared with the donor group [（0.41±0.28）% vs. （1.07±0.56）%] （P〈0.001）. Compared with lower expression group （TET2〈0.4） [（6.53±6.17）%], patients with higher expression of TET2 ≥0.4） presented significantly lower proportion of bone marrow blasts [（1.21±1.56）%1 （P〈0.05）. The expression of TET2 mRNA in BMMNC of MDS patients was inversely correlated with malignant clone burden （t=--0.398, P〈0.05） and IPSS r=-0.412, P〈0.05）. The expression of TET2 protein was down-regulated in MDS patients compared with that in the donor group. Conclusions The mRNA and protein expression of TET2 in BMMNC of MDS patients is decreased, which might be useful as an important parameter for the evaluation of MDS clone burden.

关键词： myelodysplastic syndrome TET2 gene clinical features

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Hepcidin的研究进展

中国现代医学杂志 2019年第23期29卷 38-41页

作者：王亮邵宗鸿天津医科大学第四中心临床学院天津300140 天津医科大学总医院天津300052

铁是生物体必需的元素,在氧的携带、细胞内呼吸及其他许多生物代谢途径中发挥着重要的作用。Hepcidin是调节机体铁代谢的关键激素,当机体处于慢性炎症、肿瘤等病理状态时,Hepcidin的合成增加,机体铁代谢失衡造成低铁血症,并且可致慢性... 详细信息

铁是生物体必需的元素,在氧的携带、细胞内呼吸及其他许多生物代谢途径中发挥着重要的作用。Hepcidin是调节机体铁代谢的关键激素,当机体处于慢性炎症、肿瘤等病理状态时,Hepcidin的合成增加,机体铁代谢失衡造成低铁血症,并且可致慢性病贫血(ACD)发生。许多因子如红细胞生成素(EPO)、白细胞介素-6(IL-6)、生长分化因子15(GDF15),以及红细胞铁蛋白(ERFE)均可影响Hepcidin的生成。部分肿瘤细胞可合成少量的Hepcidin,通过降低膜铁转运蛋白,限制铁排出肿瘤细胞,与肿瘤的侵袭生长密切相关,因此通过影响Hepcidin的生成来治疗ACD及肿瘤有广阔的应用前景。

关键词：慢性病贫血/贫血 Hepcidin 生长分化因子15 红细胞铁蛋白

Stable copper boron–imidazolate cage as Cu-riched enzyme for highly efficient electrocatalytic CO2 reduction to ethylene

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Science China Chemistry 2025年

作者： Jun-Qiang Chen Qiao-hong Li Qin-Long hong Ping shao Hai-Xia Zhang Jian Zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences

Single-atom catalyst has garnered widespread attention to mimic mature enzymes due to its well-defined atomic structure and coordination environments. However, since the carbon–carbon（C–C） coupling reactions require synergistic catalysis of multiple sites, single-atom catalysts suffer from insufficient active sites and unclear reaction mechanisms. Controlling the reaction intermediates in a precisely targeted pocket through careful metal–organic cage design is therefore crucial. Here, we prepare a tetrahedral [Cu6L4]-type boron–imidazolate cage integrating highly active Cu sites and optimized cavity, which exhibits enzyme like specific catalytic performance in electrochemical CO2reduction reaction（CO2RR） to enhance the selectivity of *** analyses and computational calculations suggest that the single Cu site together with neighboring boron–imidazolate ligands provides suitably synergistic effects that enable the energetically favorable formation of an *COCHO intermediate, a key step determining selectivity. As a result, the [Cu6L4]-type cage of BIC-145 achieves a Faradaic efficiency of 28% for C2H4maintaining an average current density of -3.54 mA cm-2over a 5-hour electrolysis period. This work represents the first example for studying single-metal site catalysts with ultra-low coordination numbers through the rational design of metal–organic cages.

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