检索结果-南通市图书馆

Isolation of a Plasmalemma Aquaporin Encoding Gene StPIP1 from Solanum tuberosum L. and Its Expression in Transgenic Tobacco

引用

Agricultural Sciences in China 2009年第10期8卷 1174-1186页

作者： WU Wang-ze PENG Xiao-li WANG Di Crop Research Institute Academy of Agri-Science Anhui Province Hefei 230031 P.R.China College of Agronomy Gansu Agricultural University Lanzhou 730070 P.R. China Gansu Key Lab of Crop Improvement ＆ Germplasm Enhancement Lanzhou 730070 P.R. China School of Biotechnology and Food Engineering Hefei University of Technology Hefei 230009 P.R.China

Aquaporin （AQP） belongs to a highly conserved group of membrane proteins considered as major intrinsic proteins, which facilitate water transport across biological membranes. The discovery of AQPs in plants has resulted in a paradigm shift in the understanding of plant-water relations, however, the potential relationship between the role of aquaporins in regulating plant water balance and drought tolerance still remains elusive. In this study, the gene encoding potato AQP cDNA, StPIP1 （GenBank accession no. DQ999080）, was cloned from the leaf of potato cultivar Gannongshu 2 by reverse transcription-PCR （RT-PCR）. Sequence alignment was made by BLASTn in GenBank, the phylogenetic analysis was conducted using PHYliPWY, the 3D structure was predicted in Swiss-Model server. Subcellular localization of StPIP1 was performed by constructing CaMV35S-StPIP1-GFP and rd29A-StPIP1-GFP fusion proteins and transient expression in onion epidermis. To understand StPIP1 physiological functions in potato under various stress conditions, the StPIP1 gene in a reverse orientation was transformed into tobacco driven by the Cauliflower mosaic virus （CMV） 35S promoter. The expression levels of transgenic and wild-type plants were assessed under various abiotic stress conditions using semi-quantitative RT-PCR, and the morphological and physiological responses of transgenic plants to different stress conditions were investigated. The expression of StPIP1 mRNA decreased in transgenic plants under non-stress and stress conditions, however, the reduction was more severer under drought stress. In both non-stress and stress conditions, StPIP1 was expressed predominantly in root. The morphological and physiological investigation showed no significant differences in growth rate, germination rate, and root fresh weight （FW） between transgenic and wild-type plants when grown under favorable conditions. In contrast, under drought stress, the reduction in StPIPI expression leads to a delay in seed germi

关键词： aquaporin Solanum tuberosum L. drought stress gene cloning sequence analysis gene expression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Possible Contribution of Mature γ-ray Pulsars to Cosmic-ray Positrons

引用

Chinese Journal of Astronomy and Astrophysics 2008年第1期8卷 87-95页

作者： Quan-Gui Gao ze-Jun Jiang li Zhang National Astronomical Observatories / Yunnan Observatories Chinese Academy of Sciences Kunming 650011 Department of Physics Yunnan University Kunming 650091

We restudy the possible contribution of mature gamma-ray pulsars to cosmic ray positrons based on the new version of outer gap model. In this model, the inclination angle and average properties of the outer gap are taken into account, and more mature pulsars can have the outer gap and emit high energy photons. Half of the primary particles in the outer gaps will flow back toward the star surface and emit synchrotron photons, which can produce electron/positron pairs by the cascade of pair production. Some of these pairs will escape from the light cylinder and be accelerated to relativistic energies in the pulsar wind driven by low-frequency electromagnetic waves. Using a Monte Carlo method, we obtain a sample of mature gamma-ray pulsars and then calculate the production of the positrons from these pulsars. The observed excess of cosmic positrons can be well explained by this model.

关键词： stars pulsars general - acceleration of particles - ISM cosmic rays

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of different gas releasing methods on anaerobic fermentative hydrogen production in batch cultures

引用

Frontiers of Environmental Science & Engineering 2012年第6期6卷 901-906页

作者： Sheng CHANG Jianzheng li Feng liU ze YU State Key Laboratory of Urban Water Resource and Environment School of Municipal and Environmental EngineeringHarbin Institute of TechnologyHarbin 150090China

Decreasing hydrogen partial pressure can not only increase the activity of the hydrogen enzyme but also decrease the products inhibition, so it is an appropriate method to enhance the fermentative hydrogen production from anaerobic mixed culture. The effect ofbiogas release method on anaerobic fermentative hydrogen production in batch culture system was compared, i.e., Owen method with intermediately release, continuous releasing method, and continuous releasing ＋ CO2 absorbing. The experi- mental results showed that, at 35℃, initial pH 7.0 and glucose concentration of 10 g.L-1, the hydrogen produc- tion was only 28 mL when releasing gas by Owen method, while it increased two times when releasing the biogas continuously. The cumulative hydrogen production could reach 155 mL when carbon dioxide in the gas stream was continuously absorbed by 1 mol.L-1 NaOH. The results showed that acetate was dominated, accounting for 43% in the dissolved fermentation products in Owen method, whereas the butyrate predominated and reached 47%-53% of the total liquid end products when releasing gas continuously. It is concluded that the homoacetogenesis could be suppressed when absorbing CO2 in the gas phase in fermentative hydrogen production system.

关键词： batch fermentation hydrogen production,biogas releasing hydrogen pressure homoacetogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

On Sums of Sparse Prime Subsets

引用

Acta Mathematica Sinica,English Series 2013年第8期29卷 1535-1542页

作者： Zhen CUI Hong ze li Bo Qing XUE Department of Mathematics Shanghai Jiao Tong University

For arbitrary c00, if A is a subset of the primes less than x with cardinality δx（logx）-1, δ≥（logx）-c0, then there exists a positive constant c such that the cardinality of A＋A is larger than cδx（loglogx）-1.

关键词： Density subsets subsets of the primes circle method sifted numbers

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SN 1054: A pulsar-powered supernova?

引用

Research in Astronomy and Astrophysics 2015年第11期15卷 1823-1831页

作者： Shao-ze li Yun-Wei Yu Yan Huang Institute of Astrophysics Central China Normal University

The famous ancient supernova SN 1054 could have been too bright to be explained in the “standard” radioactive-powered supernova scenario. As an alternative attempt, we demonstrate that the spin-down of the newly born Crab pulsar could provide a sufficient energy supply to make SN 1054 visible at daytime for 23 days and at night for 653 days, where a one-zone semi-analytical model is employed. Our results indicate that SN 1054 could be a “normal” cousin of magnetar-powered superluminous supernovae. Therefore, SN 1054-like supernovae could be a probe to uncover the properties of newly born neutron stars, which provide initial conditions for studies on neutron star evolutions.

关键词： scenario powered neutron gamma bright ancient luminosity attempt radioactive stars

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Band Gap Properties of Magnetoelectroelastic Grid Structures with Initial Stress

引用

Chinese Physics Letters 2012年第3期29卷 121-123页

作者： WANG Yi-ze li Feng-Ming P.O.Box 137 School of AstronauticsHarbin Institute of TechnologyHarbin 150001

The propagation of elastic waves in magnetoelectroelastic grid structures is *** gap properties are presented and the effects of the magnetoelectroelastic coupling and initial stress are *** calculations are performed using the plane-wave expansion *** results show that the band gap width can be tuned by the initial *** is hoped that our results will be helpful for designing acoustic filters with magnetoelectroelastic materials and grid structures.

关键词： designing magneto initial

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Exact Geometric Relationships, Symmetry Breaking and Structural Stability for Single-Walled Carbon Nanotubes

引用

Nano-Micro Letters 2011年第4期3卷 228-235页

作者： Tong Zhang ze Shuai Yuan li Hao Tan Research Center for Solid Mechanics Beihang University

We pioneered a study about how the geometric relationship of single-walled carbon nanotubes(SWCNT) is influenced by curvature factor and non-planar geometry factor in cylindrical coordinate system based on the assumption of complete symmetry. The bond length and angle of every carbon-carbon bonds are determined by using the principle of the minimum energy. The results of the paper include(1) From the calculation result, the symmetry breaking appears for chiral carbon nanotubes, while the part symmetry appears for achiral carbon nanotubes with increasing curvature.(2) The synergistic effect of bond lengths and bond angles is first found.(3) We conclude that the influence of non-planar geometry factor can be completely ignored on bond lengths and bond angles when the curvature parameter has been included in the model.(4)The two fractal dimensions are given from the nanoscale to the macroscale for zigzag topology and armchair topology respectively. Fractal dimensions of SWCNT show special characteristics, varying with the length of SWCNT until the lengths approach infinity. The close and inevitable correlations among curvature, symmetry breaking and stability of SWCNTs can be summed up as: the increase of curvature causes symmetry breaking,and such symmetry breaking will further reduce the structural stability.

关键词： Geometry Structural Stability Symmetry Breaking Fractal Dimension

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Study of Longitudinal-Vertical Dynamics for In-Wheel Motor-Driven Electric Vehicles

引用

Automotive Innovation 2021年第2期4卷 227-237页

作者： Yechen Qin ze Zhao Zhenfeng Wang Guofa li School of Mechanical Engineering Beijing Institute of TechnologyBeijing 100081China CAT ARC(Tianjin)Automotive Engineering Research Institute Co. LtdTianjin 300300China Institute of Human Factors and Ergonomics College of Mechatronics and Control EngineeringShenzhen UniversityShenzhen 518060China

The in-wheel motor(IWM)-driven electric vehicles(EVs)attract increasing attention due to their advantages in dimensions and *** majority of the current studies on IWM are carried out with the assumption of an ideal actuator,in which the coupling effects between the non-ideal IWM and vehicle are *** paper uses the braking process as an example to investigate the longitudinal-vertical dynamics of IWM-driven EVs while considering the mechanical-electrical coupling ***,a nonlinear switched reluctance motor model is developed,and the unbalanced electric magnetic force(UEMF)induced by static and dynamic mixed eccentricity is ***,the UEMF is decomposed into longitudinal and vertical directions and included in the longitudinal-vertical vehicle *** coupling dynamics are demonstrated under different vehicle braking scenarios;numerical simulations are carried out for various road grades,road friction,and vehicle velocities.A novel dynamics vibration absorbing system is adopted to improve the vehicle ***,the simulation results show that vehicle vertical dynamic performance is enhanced.

关键词： Mechanical-electrical coupling Longitudinal-vertical dynamics In-wheel motor Suspension system

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Supercritical fluid-assisted fabrication of C-doped Co_(3)O_(4) nanoparticles based on polymer-coated metal salt nanoreactors for efficient enzyme-mimicking and glucose sensor properties

引用

Nano Research 2023年第5期16卷 7431-7442页

作者： ze-Wen Kang Jun-Yu Zhang ze-Zhen li Ranjith Kumar Kankala Shi-Bin Wang Ai-Zheng Chen Institute of Biomaterials and Tissue Engineering Huaqiao UniversityXiamen 361021China Fujian Provincial Key Laboratory of Biochemical Technology(Huaqiao University) Xiamen 361021China Instrumental Analysis Center Laboratory and Equipment Management DepartmentHuaqiao UniversityXiamen 361021China

Nanomaterials doped with non-metallic C have attracted tremendous attention as potential nano-artificial enzymes due to their ability to change the energy band structure to improve their intrinsic ***,we report a green,facile,efficient,fast strategy to access high-performance nanozymes via supercritical CO_(2)fluid technology-fabricated polymer nanoreactor of poly-(methyl vinyl ether-co-maleic anhydride)(PVM/MA)coated Co(NO_(3))_(2)into C-doped Co_(3)O_(4)(C-Co_(3)O_(4))nanozyme by a onestep calcination *** PVM/MA to C doping into Co_(3)O_(4)shortens the entire lattice constant of the crystal structure,and the overall valence band energy level below the Fermi level shifts toward the lower energy *** as-prepared CCo_(3)O_(4)demonstrated significant peroxidase-like catalytic activity,significantly greater than the undoped Co_(3)O_(4)nanoparticle *** following density functional theory(DFT)calculations revealed that the doped nano-enzyme catalytic site displayed a unique electronic structure,altering the material surface with more electrons to fill the anti-bond of the two molecular orbitals,significantly improving the peroxidase-like enzyme catalytic and glucose sensor *** resultant enzymatic glucose sensing in a linear range of 0.1–0.6 mM with a detection limit of 3.86μM is in line with standard Michaelis–Menten ***,this work demonstrates that converting polymers into nanozymes of C-doped form by supercritical CO_(2)fluid technology in a step is an effective strategy for constructing high-performance glucose sensor *** cost-effective,reliable,precise system offers the potential for rapid analyte detection,facilitating its application in a variety of fields.

关键词： supercritical fluid poly-(methyl vinyl ether-co-maleic anhydride)(PVM/MA) C-doped Co_(3)O_(4) nanozymes density functional theory(DFT)calculation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Redshift Dependence of the Low-energy Spectral Index of Gamma-Ray Bursts Revisited

引用

Research in Astronomy and Astrophysics 2023年第12期23卷 57-62页

作者： Xiao-li Zhang Yong-Feng Huang ze-Cheng Zou Department of Physics Nanjing UniversityNanjing 210023China School of Astronomy and Space Science Nanjing UniversityNanjing 210023China Key Laboratory of Modern Astronomy and Astrophysics(Nanjing University) Ministry of EducationNanjing 210023China Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China

A negative correlation was found to exist between the low-energy spectral index and the redshift of gamma-ray bursts(GRBs)by Amati et *** was later confirmed by Geng&Huang and Gruber et al.,but the correlation was also found to be quite dispersive when the sample size was significantly *** this study,we have established two even larger samples of GRBs to further examine the *** of our samples consists of316 GRBs detected by the Swift satellite,and the other one consists of 80 GRBs detected by the Fermi *** is found that there is no correlation between the two parameters for the Swift sample,but there does exist a weak negative correlation for the Fermi *** correlation becomes even more significant when the spectral index at the peak flux is *** is argued that the absence of the correlation in the Swift sample may be due to the fact that Swift has a very narrow energy response so that it could not measure the low-energy spectral index accurately *** studies based on even larger GRB samples are solicited.

关键词： (stars:)gamma-ray burst general-methods statistical-catalogs

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：