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In-situ study of the microstructure evolution during tension of a Mg-Y-Zn-Al alloy processed by rapidly solidified ribbon consolidation technique

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Journal of Magnesium and Alloys 2024年第5期12卷 2024-2040页

作者： Jeno Gubicza Kristián Máthis Péter nagy Péter Jenei zoltán hegedus Andrea Farkas Jozef Vesely Shin-ichi Inoue Daria Drozdenko Yoshihito Kawamura Department of Materials Physics Faculty of ScienceEotvos Loránd UniversityPázmány P.sétány 1/ABudapest H-1117Hungary Department of Physics of Materials Faculty of Mathematics and PhysicsCharles UniversityKe Karlovu 512116 Praha 2Czech Republic Deutsches Elektronen-Synchrotron DESY Notkestr.85Hamburg 22603Germany Magnesium Research Center Kumamoto University2-39-1 KurokamiChuo-kuKumamoto 860-8555Japan

Mg-Y-Zn-Al alloys processed by rapidly solidified ribbon consolidation(RSRC)technique exhibit an exceptional mechanical performance indicating promising application *** material has a bimodal microstructure consisting of fine recrystallized and coarse non-recrystallized grains with solute-rich stacking faults forming cluster arranged layers(CALs)and nanoplates(CAnaPs),or complete long period stacking ordered(LPSO)*** order to reveal the deformation mechanisms,in-situ synchrotron X-ray diffraction line profile analysis was employed for a detailed study of the dislocation arrangement created during tension in Mg-0.9%Zn-2.05%Y-0.15%Al(at%)*** uncovering the effect of the initial microstructure on the mechanical performance,additional samples were obtained by annealing of the as-consolidated specimen at 300 and 400℃ for 2 *** heat treatment at 300℃ had no significant effect on the initial microstructure,its evolution during tension and,thus,the overall deformation behavior under tensile *** the other hand,annealing at 400℃ resulted in a significant increase of the recrystallized grains fraction and a decrease of the dislocation density,leading to only minor degradation of the mechanical *** maximum dislocation density at the failure of the samples corresponding to the plastic strain of 10-25% was estimated to be about 16-20×10^(14)m^(-2).The diffraction profile analysis indicated that most dislocations formed during tension were of non-basal and pyramidal types,what was also in agreement with the Schmid factor values revealed independently from orientation *** was also shown that the dislocation-induced Taylor hardening was much lower below the plastic strain of 3% than above this value,which was explained by a model of the interaction between prismatic dislocations and CAnaPs/LPSO plates.

关键词： Mg-Zn-Y-Al alloy Long period stacking ordered(LPSO)phase Cluster arranged nanoplates(CAnaPs) Annealing Tension Dislocation density hardening

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Methionine sources and genotype affect embryonic intestinal development,antioxidants,tight junctions,and growth-related gene expression in chickens

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Animal nutrition 2024年第1期16卷 218-230页

作者： James K.Lugata Sawadi F.ndunguru Gebrehaweria K.Reda Xénia E.Ozsváth Eszter Angyal Levente Czeglédi Gabriella Gulyás Renáta Knop János Oláh zoltán Mészár Rita Varga Brigitta Csernus Csaba Szabó Department of Animal nutrition and Physiology Faculty of Agriculture and Food Sciences and Environmental ManagementUniversity of DebrecenDebrecenHungary Department of Animal husbandry Faculty of Agriculture and Food Sciences and Environmental ManagementUniversity of DebrecenDebrecenHungary Faculty of Agriculture and Food Sciences and Environmental Management Doctoral School of Animal ScienceUniversity of DebrecenDebrecenHungary Institutes for Agricultural Research and Educational Farm University of DebrecenDebrecenHungary Department of Anatomy Histology and EmbryologyFaculty of MedicineUniversity of DebrecenDebrecenHungary Department of Evolutionary Zoology and human Biology Faculty of Science and TechnologyUniversity of DebrecenDebrecenHungary

Methionine(Met)is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and *** present study examined the effect of in ovo injection of DL-Met and L-Met sources and genotypes on chicken embryonic-intestinal development and *** eggs of the two genotypes,TETRA-SL layer hybrid(TSL)—commercial layer hybrid and hungarian Partridge colored hen breed(hPC)—a native genotype,were randomly distributed into four treatments for each *** treatment groups include the following:1)control non-injected eggs(noIn);2)saline-injected(SaIn);3)DL-Met injected(DLM);and 4)L-Met injected(LM).The in ovo injection was carried out on 17.5 d of embryonic development;after hatching,eight chicks per group were sacrificed,and the jejunum was extracted for *** results showed that both DLM and LM groups had enhanced intestinal development as evidenced by increased villus width,villus height,and villus area(Phe *** DLM group had significantly reduced crypt depth,glutathione(GSh)content,glutathione S-transferase 3 alpha(GST3),occludin(OCLn)gene expression and increased villus height to crypt depth ratio in the TSL genotype than the LM group(Phe hPC genotype has overexpressed insulin-like growth factor 1(IGF1)gene,tricellulin(MD2),occludin(OCLn),superoxide dismutase 1(SOD1),and GST3 genes than the TSL genotype(Pn conclusion,these findings showed that in ovo injection of Met enhanced intestinal development,and function,with genotypes responding differently under normal *** also influenced the expression of intestinal antioxidants,tight junction,and growth-related genes.

关键词： DL-Methionine L-Methionine In ovo injection Intestinal development Poultry

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Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells

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World Journal of Gastroenterology 2006年第11期12卷 1730-1738页

作者： Károly Palatka zoltán Serf(o|″)z(o|″) zoltán Veréb Róbert Bátori Beáta Lontay zoltán h.rgitay zoltán nemes Miklós Udvardy Ferenc Erd(o|″)di István Altorjay Department of Gastroenterology University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Department of Experimental Zoology BLRI-hAS TihanyHungary Institute of Immunology University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001 Hungary Department of Medical Chemistry University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Department of Pathology Gyula Kenezy hospital DebrecenBartók Béla út 10.Debrecen H-4026Hungary Institute of Pathology University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Institute of Internal Medicine University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary

AIM： To study the expression of endothelial and inducible nitric oxide synthases （enOS and inOS） and their role in inflammatory bowel disease （IBD）. METhODS： We examined the effect of sera obtained from patients with active Crohn＇s disease （CD） and ulcerative colitis （UC） on the function and viability of human umbilical vein endothelial cells （hUVEC）. hUVECs were cultured for 0-48 h in the presence of a medium containing pooled serum of healthy controls, or serum from patients with active CD or UC. Expression of enOS and inOS was visualized by immunofluorescence, and quantified by the densitometry of Western blots. Proliferation activity was assessed by computerized image analyses of Ki-67 immunoreactive cells, and also tested in the presence of the nOS inhibitor, 10^-4 mol/L L-nAME. Apoptosis and necrosis was examined by the annexin-V-biotin method and by propidium iodide staining, respectively. RESULTS： In hUVEC immediately after exposure to UC, serum enOS was markedly induced, reaching a peak at 12 h. In contrast, a decrease in enOS was observed after incubation with CD sera and the enOS level was minimal at 20 h compared to control （18%±16% vs 23%± 15% P〈0.01）. UC or CD serum caused a significant increase in inOS compared to control （UC： 300%±21%; CD： 275% ± 27% vs 108% ± 14%, P〈0.01）. Apoptosis/necrosis characteristics did not differ significantly in either experiment. Increased proliferation activity was detected in the presence of CD serum or after treatment with L-nAME. Cultures showed tube-like formations after 24 h treatment with CD serum. COnCLUSIOn： IBD sera evoked changes in the ratio of enOS/inOS, whereas did not influence the viability of hUVEC. These involved down-regulation of enOS and up-regulation of inOS simultaneously, leading to increased proliferation activity and possibly a reduced antiinflammatory protection of endothelial cells.

关键词： Crohn's disease human umbilical vein endothelial cells Inflammatory bowel disease nitric oxide synthase Ulcerative colitis

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Stone–Wales defects in hexagonal boron nitride as ultraviolet emitters

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npj Computational Materials 2020年第1期6卷 169-175页

作者： hanen hamdi GergőThiering zoltán Bodrog Viktor Ivády Adam Gali Wigner Research Centre for Physics P.O.Box 49Budapest 1525Hungary Department of Physics Chemistry and BiologyLinköping UniversityLinköping SE-58183Sweden Budapest University of Technology and Economics Budafokiút 8.Budapest 1111Hungary

Many quantum emitters have been measured close or near the grain boundaries of the two-dimensional hexagonal boron nitride where various Stone–Wales defects *** show by means of first principles density functional theory calculations that the pentagon–heptagon Stone–Wales defect is an ultraviolet emitter and its optical properties closely follow the characteristics of a 4.08-eV quantum emitter,often observed in polycrystalline hexagonal boron *** also show that the square–octagon Stone–Wales line defects are optically active in the ultraviolet region with varying gaps depending on their density in hexagonal boron *** results may introduce a paradigm shift in the identification of fluorescent centres in this material.

关键词： Wales boron hexagonal

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Endangered lowland oak forest steppe remnants keep unique bird species richness in Central hungary

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Journal of Forestry Research 2022年第1期33卷 343-355页

作者： GáborÓnodi zoltán Botta‑Dukát Dániel Winkler Tamás Rédei Institute of Ecology and Botany Centre for Ecological ResearchVácrátótHungary Department of Botany and Biodiversity Research University of ViennaViennaAustria Institute of Wildlife Management and Wildlife Biology University of SopronSopronHungary

The biodiversity of natural or semi-natural native,old oak woodlands have high conservation importance,especially in landscapes of monocultural forest plantations and arable *** a wider variety of microhabitats and foraging sources,such old oak forests can provide essential habitat for native forest bird *** conducted a study using bird point counts to compare the forest bird communities of old pedunculate oak(Quercus robur)remnants with native and non-native plantations in central hungary in a landscape of mostly arable fields,settlements,and monocultural *** surveys were carried out in old oak forest remnants,middle-aged oak,white poplar(Populus alba),hybrid poplar(Populus×euramericana),black locust(Robinia pseudoacacia),and pine(Pinus spp.)*** has been carried out in nine study sites,where all six habitat types were represented(with a few exceptions),to determine total abundance,species richness,Shannon–Wiener diversity,species evenness,dominant and indicator species,and guild *** found that old oak forest remnants were the most diverse habitats among the studied forest types,while hybrid poplar and pine plantations exhibited the lowest avian *** avian guilds most sensitive to the loss of old oak forest remnants were ground foragers,bark foragers,cavity-nesters,residents,and Mediterranean migratory *** habitats were more diverse than non-native *** results suggest that it is important to conserve all remaining high biodiversity old oak stands and to avoid clear-cutting of monocultural plantations in favour of practices such as mixed-species plantations,longer rotation lengths,or retention forestry.

关键词： Forest bird community Bird conservation Old oak forest-steppe remnant Plantation forestry Forest conservation non-native plantation

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Ethanol inhibits the motility of rabbit sphincter of Oddi in vitro

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World Journal of Gastroenterology 2004年第23期10卷 3470-3474页

作者： Réka Sári Attila Pálv lgyi zoltán Rakonczay Jr Tamás Takács János Lonovics László Czakó Péter hegyi zoltán Szilvássy First Department of Medicine Faculty of MedicineUniversity of Szeged H-6720Koranyi fasor 10Hungary Department of Pharmacology Medical University of Debrecen H-4032Nagyerdei krt98Hungary

AIM:The rote of the sphincter of Oddi(SO)in ethanol (ETOh)-induced pancreatitis is *** aim was to characterise the effect of E-I-Oh on basal and stimulated SO motility. METhODS:SOs removed from white rabbits were placed in an organ bath(Krebs solution,ph7.4,37℃).The effects of 2 mL/L,4 mL/L,6 mL/L and 8 mL/L of ETOh on the contractile responses of the sphincter were determined. SOs were stimulated with either 0.1 μmol/L carbachol,1 μmol/L erythromycin or 0.1 μmol/L cholecystokinin(CCK). RESULTS:ETOh at a dose of 4 mL/L significantly decreased the baseline contractile amplitude from 11.98±0.05 mn to 11.19±0.07 ***,no significant changes in the contractile frequency were ***(0.6%) significantly decreased both the baseline amplitude and the frequency compared to the control group(10.50±0.01 mn, 12.13±0.10 mn and 3.53±0.13 c/min,5.5±0.13 cycles(c)/min, respectively).Moreover,0.8% of ETOh resulted in complete relaxation of the ***(0.1 μmol/L)or erythromycin (1 μmol/L)stimulated the baseline amplitudes(by 82% and 75%,respectively)and the contractile frequencies (by 150% and 106%,respectively).In the carbachol or erythromydn-stimulated groups 2-6 mL/L of E-IOh significantly inhibited both the amplitude and the ***, a 4-5 min administration of 6 mL/L ETOh suddenly and completely relaxed the ***(0.1 μmol/L)stimulated the baseline amplitude from 12.37±0.05 mn to 27.40±1.82 mn within 1.60±0.24 *** this peak,the amplitude decreased to 17.17±0.22 mn and remained constant during the *** frequency peaked at 12.8±0.2 c/min, after which the constant frequency was 9.43±0.24 c/min throughout the rest of the *** at a dose of 4 mL/L significantly decreased the amplitude from 16.13±0.23 mn to 14.93±0.19 ***,no significant changes in the contractile frequency were *** at a dose of 6 mL/L inhibited both the amplitudes and the frequencies in the CCK-stimulated group,while 8 mL/L of E

关键词： Animals Carbachol Central nervous System Depressants Cholecystokinin Cholinergic Agonists Erythromycin Ethanol Gastrointestinal Agents Gastrointestinal Motility In Vitro Male Rabbits Research Support, non-U.S. Gov't Sphincter of Oddi

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Is rectal indomethacin effective in preventing of post-endoscopic retrograde cholangiopancreatography pancreatitis?

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World Journal of Gastroenterology 2014年第29期20卷 10151-10157页

作者： zoltán Dbrnte zoltán Szepes Ferenc Izbéki Judit Gervain László Lakatos Gyula Pécsi Miklós Ihász Lilla Lakner Erzsébet Toldy László Czakó Department of Gastroenterology and Internal Medicine Markusovszky Teaching Hospital 1st Department of Medicine University of Szeged 1st Department of Internal Medicine Szent Gyrgy Regional Hospital Department of Internal Medicine Csolnoky Ferenc Regional Hospital Department of Gastroenterology Karolina Hospital Central Laboratory Markusovszky Teaching Hospital Institute of Diagnostics Faculty of Health SciencesUniversity of Pécs

AIM: To investigate the effectiveness of rectally administered indomethacin in the prophylaxis of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis and hyperamylasaemia in a multicentre study.

关键词： Endoscopic retrograde cholangiopancreatography Post-endoscopic retrograde cholangiopancreatography pancreatitis hyperamylasaemia nonsteroidal anti-inflammatory drugs Indomethacin

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Interplay between nitric oxide and VIP in CCK-8-induced phasic contractile activity in the rabbit sphincter of Oddi

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World Journal of Gastroenterology 2005年第21期11卷 3264-3266页

作者： Attila Pálv(o|¨)lgyi Réka Sári József németh Annamária Szabolcs István nagy Péter hegyi János Lonovics zoltán Szilvássy First Department of Medicine Faculty of Medicine University of SzegedH-6720 SzegedKorányi fasor 8-10 Hungary Department of Pharmacology Medical University of Pécs H-7643 Pécs Szigeti u. 12 Hungary First Department of Medicine Faculty of Medicine University of Szeged H-6720 Szeged Korányi fasor 8-10 Hungary Department of Pharmacology Medical University of Debrecen H-4032 DebrecenNagyerdei krt.98Hungary

AIM: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (nO) were involved in phasic contractile activity of the rabbit SO stimulated by cholecystokinin-octapeptide (CCK-8). METhODS: Isolated SO muscle rings were cleaned of fat and mounted horizontally on two small L-shaped hooks one of which was connected to a force transducer for the measurement of isometric *** experiments were carried out in a thermostatically controlled (37±0.2℃) organ bath (5 mL) containing Krebs *** organ fluid was gassed with 95% O2 and 50 mL/L CO2 to keep the ph at 7.40±0.05. Contractile responses to CCK-8 (1 μmol/L) were evaluated in the presence and absence of nG-nitro-L-arginine (LnnA), an inhibitor of nO synthase (100 μmol/L), and (p-chloro-D-Phe6-Leu17)-VIP (VlPa, 30 μmol/L), a VIP receptor antagonist. RESULTS: CCK-8 stimulated the phasic activity of the SO. nO synthase inhibition increased the frequency and amplitude of contractions with a slight increase in developed tension. Pre-incubation with VlPa also attenuated this CCK-8 effect. The combined application of LnnA and VlPa abolished the phasic activity of the muscle rings with a marked increase in tension in response to CCK-8. COnCLUSIOn: VIP and nO together contribute to an increase in phasic activity of SO.

关键词： Sphincter of Oddi CCK VIP nO LnnA

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Characterization of h^+ and hCO_3^- transporters in CFPAC-1 human pancreatic duct cells

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World Journal of Gastroenterology 2006年第6期12卷 885-895页

作者： zoltánRakonczayJr AmyFearn Péterhegy MichaelAGray BarryEArgent Péterhegyi Imre Boros institute for Cell anti Molecular Biosciences.University of newcastle upon Tyne Medical SchoolNewcastle upon Tyne NE2 4HHUnited Kingdom First Department of Medicine Faculty of MedicineUniversity of SzegedH-6720 Szeged institute for Cell anti Molecular Biosciences.University of newcastle upon Tyne Medical SchoolNewcastle upon Tyne NE2 4HH hungarian Academy of Sciences Biological Research CenterInstitute of BiochemistryH-6726 Szeged

AIM： To characterize h^＋ and hCO3^- transporters in polarized CFPAC-1 human pancreatic duct cells, which were derived from a cystic fibrosis patient with the AF508 CFTR mutation. METhODS： CFPAC-1 cells were seeded at high density onto permeable supports and grown to confluence. The cells were loaded with the ph-sensitive fluorescent dye BCECF, and mounted into a perfusion chamber, which allowed the simultaneous perfusion of the basolateral and apical membranes. Transmembrane base flux was calculated from the changes in intracellular ph and the buffering capacity of the cells. RESULTS： Our results showed differential permeability to hCO3^＋/CO2 at the apical and basolateral membranes of CFPAC-1 cells. na^＋/hCO3^- co-transporters （nBCs） and Cl^-/hCO3^- exchangers （AEs） were present on the basolateral membrane, and na＋/h＋ exchangers （nhEs） on both the apical and basolateral membranes of the cells. Basolateral hCO3 uptake was sensitive to variations of extracellular K^＋ concentration, the membrane permeable carbonic anhydrase （CA） inhibitors acetazolamide （100 μmol/L） and ethoxyzolamide （100 μmol/L）, and was partially inhibited by h2-DIDS （600 μmol/L）. The membrane-impermeable CA inhibitor 1-n-（4-sulfamoylphenylethyl）-2,4,6-trimethylpyridine perchlorate did not have any effect on hCO3^- *** basolateral AE had a much higher activity than that in the apical membrane, whereas there was no such difference with the nhE under resting conditions. Also, 10 μmol/L forskolin did not significantly influence Cl^-/hCO3^- exchange on the apical and basolateral membranes. The administration of 250 μmol/L h2-DIDS significantly inhibited the basolateral AE. Amiloride （300 μmol/L） completely inhibited nhEs on both membranes of the cells. RT-PCR revealed the expression of pnBC1, AE2, and nhE1 mRnA. COnCLUSIOn： These data suggest that apart from the lack of CFTR and apical Cl^-/hCO3^- exchanger activity, CFPAC-1 cells express similar h^＋ and hCO3^- transporters to those observe

关键词： CFPAC-1 Pancreatic duct cells hCO3^- Intracellular ph

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Insulin is necessary for the hypertrophic effect of cholecystokinin-octapeptide following acute necrotizing experimental pancreatitis

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World Journal of Gastroenterology 2004年第15期10卷 2275-2277页

作者： Péter hegyi zoltán RakonczayJr Réka Sári László Czakó norbert Farkas Csaba Góg József németh János Lonovics Tamás Takács First Department of Medicine Faculty of MedicineUniversity of Szeged Szeged H6722Hungary School of Cell and Molecular BiosciencesMedical SchoolUniversity of NewcastleNewcastle upon TyneNE2 4HHUnited Kingdom First Department of Medicine Faculty of MedicineUniversity of Szeged Szeged H6722Hungary Jr.School of Cell and Molecular BiosciencesMedical SchoolUniversity of NewcastleNewcastle upon TyneNE2 4HHUnited Kingdom First Department of Medicine Faculty of MedicineUniversity of Szeged Szeged H6722Hungary

AIM:In previous experiments we have demonstrated that by administering low doses of cholecystokinin-octapeptide (CCK-8),the process of regeneration following L-arginine (Arg)-induced pancreatitis is *** rats that were also diabetic(induced by streptozotocin,STZ),pancreatic regeneration was not *** aim of this study was to deduce whether the administration of exogenous insulin could in fact restore the hypertrophic effect of CCK-8 in diabetic-pancreatitic rats. METhODS:Male Wistar rats were used for the experiments. Diabetes mellitus was induced by administering 60mg/kg body mass of STZ intraperitoneally(i.p.),then,on d 8, pancreatitis was induced by 200mg/100 g body mass Arg *** at an interval of 1 *** animals were injected subcutaneously twice daily(at 7 *** 7 p.m.)with 1 μg/kg of CCK-8 and/or 2 IU mixed insulin(300g/L short- action and 700g/L intermediate-action insulin) for 14 d after pancreatitis *** this the animals were killed and the serum amylase,glucose and insulin levels as well as the plasma glucagon levels,the pancreatic mass/body mass ratio(pm/bm),the pancreatic contents of DnA,protein,amylase,lipase and trypsinogen were *** tissue samples were examined by light microscopy on paraffin-embedded sections. RESULTS:In the diabetic-pancreatitic rats treatment with insulin and CCK-8 significantly elevated pw/bm and the pancreatic contents of protein,amylase and lipase vs the rats receiving only CCK-8 ***-8 administered in combination with insulin also elevated the number of acinar cells with mitotic activities,whereas CCK-8 alone had no effect on laboratory parameters or the mitotic activities in diabetic-pancreatitic rats. COnCLUSIOn:Despite the hypertrophic effect of CCK-8 being absent following acute pancreatitis in diabetic-rats, the simultaneous administration of exogenous insulin restored this *** results clearly demonstrate that insulin is necessary for the hypertrophic ef

关键词： Animals Diabetes Mellitus, Experimental hypoglycemic Agents Insulin Male Pancreatitis, Acute necrotizing Rats Rats, Wistar Regeneration Research Support, non-U.S. Gov't Sincalide

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