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Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison with the standard method of culture(Most probably Number)

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Asian Pacific Journal of Tropical Biomedicine 2014年第5期4卷 404-409页

作者： Dehghan fatemeh zolfaghari mohammad reza Arjomandzadegan mohammad Kalantari Salomeh Ahmari Gholam reza Sarmadian Hossein Sadrnia Maryam Ahmadi Azam Shojapoor Mana Najarian Negin Kasravi Alii reza Falahat Saeed Department of Microbiology Qom BranchIslamic Azad University Tuberculosis and Infectious Research Center and Department of Microbiology Arak University of Medical Sciences Hygiene and Quality Control Office of Markazi Province Water and Wastewater Company Department of Biology Payame Noor University Molecular and Medicine Research Center Arak University of Medical Sciences Department of Microbiology Arak University of Medical Sciences Department of Microbiology Islamic Azad UniversitySciences and Research Branch

Objective:To analyse molecular detection of coliforms and shorten the time of ***:Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably number(MPN)method for 16 artificial and 101 field *** molecular method was also conducted on isolated coliforms from positive MPN samples;standard sample for verification of microbial method certificated reference material;isolated strains from certificated reference material and standard *** PCR and electrophoresis parameters were changed for reducing the operation ***:Results of PCR for lacZ and uidA genes were similar in all of standard,operational and artificial samples and showed the 876 bp and 147 bp bands of lacZ and uidA genes by multiplex *** results were confirmed by MPN culture method by sensitivity 86%(95%CI:0.71-0.93).Also the total execution time,with a successful change of factors,was reduced to less than two and a half ***:Multiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak ***'s recommended to be used at least as an initial screening test,and then the positive samples could be randomly tested by MPN.

关键词： Water Coliforms PCR Rapid detection

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A GIS-based time-dependent seismic source modeling of Northern Iran

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Earthquake Engineering and Engineering Vibration 2017年第1期16卷 33-45页

作者： Mahdi Hashemi Ali Asghar Alesheikh mohammad reza zolfaghari School of Information Seiences University of Pittsburgh Pittsburgh Pennsylvania 15260 USA Department of Geospatial Information Systems K.N. Toosi University of Technology Tehran 19697 Iran Civil Engineering Department K.N. Toosi Universiry of Technology Tehran 19697 Iran

： The first step in any seismic hazard study is the definition of seismogenic sources and the estimation of magnitude-frequency relationships for each source. There is as yet no standard methodology for source modeling and many researchers have worked on this topic. This study is an effort to define linear and area seismic sources for Northern Iran. The linear or fault sources are developed based on tectonic features and characteristic earthquakes while the area sources are developed based on spatial distribution of small to moderate earthquakes. Time-dependent recurrence relationships are developed for fault sources using renewal approach while time-independent frequency-magnitude relationships are proposed for area sources based on Poisson process. GIS functionalities are used in this study to introduce and incorporate spatial- temporal and geostatistical indices in delineating area seismic sources. The proposed methodology is used to model seismic sources for an area of about 500 by 400 square kilometers around Tehran. Previous researches and reports are studied to compile an earthquake/fault catalog that is as complete as possible. All events are transformed to uniform magnitude scale; duplicate events and dependent shocks are removed. Completeness and time distribution of the compiled catalog is taken into account. The proposed area and linear seismic sources in conjunction with defined recurrence relationships can be used to develop time-dependent probabilistic seismic hazard analysis of Northern Iran.

关键词： seismic source modeling geostatistical index seismic hazard GIS

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Interleukin-12 as a Genetic Adjuvant Enhances Hepatitis C Virus NS3 DNA Vaccine Immunogenicity

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Virologica Sinica 2013年第3期28卷 167-173页

作者： Malihe Naderi Atefeh Saeedi Abdolvahab Moradi Mishar Kleshadi mohammad reza zolfaghari Ali Gorji Amir Ghaemi Department of Microbiology Qom branch Islamic Azad University Department of Microbiology Lahijan Branch Islamic Azad University Golestan Research Center of Gastroenterology and Hepatology-GRCGH Department of Microbiology Golestan University of Medical Sciences Shefa Neuroscience Research Centre Institut für Physiologie I Westflische Wilhelms-Universitt Münster Klinik und Poliklinik für Neurochirurgie Westf lische Wilhelms-Universitt Münster

Hepatitis C virus (HCV) chronic infection is a worldwide health problem, and numerous efforts have been invested to develop novel vaccines. An efficient vaccine requires broad immune response induction against viral proteins. To achieve this goal, we constructed a DNA vaccine expressing nonstructural 3 (NS3) gene (pcDNA3.1-HCV-NS3) and assessed the immune response in C57BL/6 mice. In this study, the NS3 gene was amplified with a nested-reverse transcriptase-polymerase chain reaction (RT-PCR) method using sera of HCV-infected patients with genotype 1a. The resulting NS3 gene was subcloned into a pcDNA3.1 eukaryotic expression vector, and gene expression was detected by western blot. The resultant DNA vaccine was co-administered with interleukin-12 (IL-12) as an adjuvant to female C57BL/6 mice. After the final immunizations, lymphocyte proliferation, cytotoxicity, and cytokine levels were assessed to measure immune responses. Our data suggest that co-administration of HCV NS3 DNA vaccine with IL-12 induces production of significant levels of both IL-4 and interferon (IFN)-γ (p<0.05). Cytotoxicity and lymphocyte proliferation responses of vaccinated mice were significantly increased compared to control (p<0.05). Collectively, our results demonstrated that co-administration of HCV NS3 and IL-12 displayed strong immunogenicity in a murine model.

关键词： Hepatitis C virus (HCV) NS3 Interleukin-12 DNA vaccine

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Prediction of peak ground acceleration of Iran's tectonic regions using a hybrid soft computing technique

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Geoscience Frontiers 2016年第1期7卷 75-82页

作者： Mostafa Gandomi Mohsen Soltanpour mohammad R.zolfaghari Amir H.Gandomi Department of Civil Engineering Khajeh Nasir Toosi University of Technology Department of Civil Engineering The University of Akron

A new model is derived to predict the peak ground acceleration（PGA） utilizing a hybrid method coupling artificial neural network（ANN） and simulated annealing（SA）, called SA-ANN. The proposed model relates PGA to earthquake source to site distance, earthquake magnitude, average shear-wave velocity,faulting mechanisms, and focal depth. A database of strong ground-motion recordings of 36 earthquakes,which happened in Iran’s tectonic regions, is used to establish the model. For more validity verification,the SA-ANN model is employed to predict the PGA of a part of the database beyond the training data domain. The proposed SA-ANN model is compared with the simple ANN in addition to 10 well-known models proposed in the literature. The proposed model performance is superior to the single ANN and other existing attenuation models. The SA-ANN model is highly correlated to the actual records（R=0.835 and r =0.0908） and it is subsequently converted into a tractable design equation.

关键词： Peak ground acceleration Artificial neural networks Simulated annealing Explicit formulation

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Mobile blood collection sites and their roles in providing safe andadequate supply： A six-year experience

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Frontiers in Biology 2018年第3期13卷 226-234页

作者： Mostafa Paridar Abbas Khosravi mohammad-Ali Jalali-Far Sima zolfaghari Omid Kiani Ghaleh Sardi Mehdi Sajadi Deputy of Management and Resources Development Ministry of Health and Medical Education Tehran Iran Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran Department of Hematolog Iran University of Medical Sciences Tehranlran Faculty of Allied Medicine Bitjand University of Medical Sciences Birjand Iran

The determination of the role of mobile sites, as compared with fixed sites, in providing safe blood supply will help with the planning of future programs. This retrospective study was carried out at the Khuzestan Blood Transfusion Organization from 2007 to 2012. Samples of the blood collected at mobile sites and fixed sites were compared. Comparisons took into consideration noticeable trends as well as the prevalence of major TTIs including HIV, HBV and HCV. The total number of blood donations from 2007 to 2012 was 621117 out of which 89590 (14.43%) were collected from mobile sites. The overall blood donation index was estimated at 23.8 per 1000 population. The prevalence of HIV, HBV and HCV in mobile site donations was 5.31, 320.34 and 117.4, and in fixed sites was 5.31, 214.72 and 104.83 per 100000 donations respectively. HBV prevalence in mobile sites was significantly higher than in fixed sites (p = 0.014). The blood donation index in Khuzestan province is much better when compared with areas of similar socioeconomic status as well as neighboring countries. The allotment of blood units collected by mobile teams is lower than that of national reports. In addition, the prevalence of TTIs in mobile site blood donations was higher than at fixed sites.

关键词： fixed sites, mobile sites, blood donations, TTIs, Iran

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