检索结果-南通市图书馆

Association between Polymorphisms of Glutathione S-Transferase and Progression to Cervical Cancer in Women from Burkina Faso and Mali

引用

Journal of Biosciences and Medicines 2020年第4期8卷 12-25页

作者： Teega-Wendé Clarisse Ouedraogo Florencia Wendkuuni djigma théodora mahoukèdè zohoncon Boureima Idani Abdoul Karim Ouattara Pegdwendé Abel Sorgho dorcas Obiri-Yeboah Prosper Bado Mah Alima Esther Traore Birama diarra Albert théophane Yonli Charlemagne Ouedraogo Jacques Simpore Laboratory of Molecular Biology and Genetics (LABIOGENE) University Joseph Ki-Zerbo Ouagadougou Burkina Faso Pietro Annigoni Biomolecular Research Centre (CERBA) Ouagadougou Burkina Faso University Saint thomas d’Aquin Faculty of Medicine Ouagadougou Burkina Faso department of Microbiology and Immunology School of Medical Sciences University of Cape Coast PMB Cape Coast Ghana University Joseph Ki-Zerbo UFR/SDS Ouagadougou Burkina Faso

Although persistence of high-risk human papillomavirus infection is the main risk factor, Glutathione S-Transferase highly polymorphic enzyme involved in the metabolism of xenobiotics, is a good candidate gene. the objective of this study was to compare the polymorphisms of Glutathione S-Transferase M1-null in women with cancerous lesions and without lesions. this study consisted of 322 uterine cervix samples of women from Mali and Burkina Faso with Cervical Intra-epithelial Neoplasia 2 and 3, adenocarcinoma and squamous cell carcinoma and 100 women with no lesions. Human Papillomavirus genotyping was performed by Real-time multiplex Polymerase Chain Reaction. Glutathione S-Transferase gene polymorphisms were determined using conventional Polymerase Chain Reaction followed by migration on agarose gel. A statistically significant association with high relative risks of 10.77 for the development of High grade Superficial or Squamous Intra-epithelial Lesion (95% CI = 5.59 - 20.72;p d 13.20 for cancer development (95% CI = 6.79 - 25.63;p d in women with the null genotype of Glutathione S-Transferase M1 in the study population. In Burkina Faso and Mali, Glutathione S-Transferase M1-null presented relative risks of 9 and 11.05 for high-grade lesions, 15 and 11.40 for cancer. Similarly, significant results had been observed in women with human papillomavirus positive and human papillomavirus negative. the results of the present study support the idea that the deletion of Glutathione S-Transferase M1 plays a crucial role in the progression of high-grade lesions and cervical cancer.

关键词： Glutathione S-Transferase M1-Null Cervical Cancer Burkina Faso Mali

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

detection of Human Papillomavirus dNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso

引用

American Journal of Molecular Biology 2023年第4期13卷 276-290页

作者： théodora mahoukèdè zohoncon Shoukrat Ohuwa Toyin Bello Prosper Bado Rogomenoma Alice Ouédraogo Estelle Ouédraogo Ina Marie Angèle Traoré Abdoul Karim Ouattara Florencia Wenkunni djigma Albert théophane Yonli Assita Sanou-Lamien Olga Mélanie Lompo Jacques Simpore Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Centre Hospitalo-Universitaire Yalgado Ouedraogo Ouagadougou Burkina Faso Pathological Anatomy and Cytology Laboratory Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Introduction: Cervical cancer, caused by persistent high-risk human papillomavirus (HPV) infection, remains a global public health problem. the cellular transformation and maintenance of the malignant phenotype of these HPVs are attributed to the viral oncoproteins E6 and E7. Objective: this study aims to detect the presence of human papillomavirus dNA and E6/E7 oncoprotein mRNA of HPV genotypes 16, 18, 31 and 33 in cases of cervical cancer and precancerous lesions, histologically confirmed in Burkina Faso. Methods: this descriptive cross-sectional study focused on cases of cervical cancer and high-grade intraepithelial neoplasia (CIN) and was conducted from June to december 2022. One hundred (100) samples of fixed and paraffin-embedded tissues were collected from the pathological anatomy and cytology laboratories of hospitals in the capital of Burkina Faso. High-risk human papillomavirus (HR-HPV) dNA was detected using multiplex real-time PCR, while the presence of E6 and E7 mRNA in cervical cancer and high-grade CIN samples was determined using real-time Reverse Transcriptase-PCR (RT-PCR) with TaqMan probes. Results: the mean age of women diagnosed with cervical cancer and high-grade CIN was 50.81 ± 13.65 years, ranging from 22 to 82 years. Cervical cancer and high-grade CIN were positive for at least one high-risk human papillomavirus (HR-HPV) in 80% of cases. the most prevalent genotypes observed were HPV16, 18, 31, and 33, collectively accounting for 70.08% of cases. Of the 89 samples that tested positive for HR-HPV genotypes 16, 18, 31, and 33, 88 (98.88%;95% CI: [94.58 - 99.94]) were also positive for the presence of mRNA encoding the E6 and E7 oncoproteins of HPV16, 18, 31, and 33. Conclusion: In the presence of HPV dNA, testing for E6 and E7 oncoprotein mRNA could serve as a promising biomarker and valuable tool for improved assessment of the progression to cervical cancer.

关键词： HPV E6/E7 Cervical Cancer Precancerous Lesions Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

distribution of High-Risk Human Papillomavirus Genotypes in Precancerous Cervical Lesions in Ouagadougou, Burkina Faso

引用

Open Journal of Obstetrics and Gynecology 2016年第4期6卷 196-204页

作者： Charlemagne Ouédraogo théodora mahoukèdè zohoncon Esther M. A. Traoré Souleymane Ouattara Prosper Bado Clarisse T. Ouedraogo Florencia W. djigma djénéba Ouermi dorcas Obiri-Yeboah Olga Lompo Simon A. Akpona Jacques Simpore Pietro Annigoni Biomolecular Research Center (CERBA) Ouagadougou Burkina Faso UFR/SdS University of Ouagadougou Ouagadougou Burkina Faso Laboratory of Molecular Biology and Genetics (LABIOGENE) University of Ouagadougou Ouagadougo Burkina Faso Teaching and Research Unit in Biochemistry and Molecular Biology Faculty of Medicine University of Parakou Parakou Republic of Benin Faculty of Medicine Université Saint Thomas d’Aquin Ouagadougou Burkina Faso University of Cape Coast School of Medical Sciences Microbiology Department University Post Office Cape Coast Ghana Yalgado Ouédraogo University Hospital (CHU/YO) Ouagadougou Burkina Faso

Aims: We aimed at identifying the high-risk HPV genotypes associated with high-grade dysplastic cervical lesions in Burkina Faso. the available vaccines to Burkina Faso only protect against two high risk HPV genotypes: HPV 16 and 18. Are the genotypes identified in the high-grade precan-cerous lesions in this survey covered by the available vaccines? Methods: the detection and genotyping of high-risk HPV have been conducted based on 118 formalin-fixed and paraffin-embedded archived tissues using the “HPV Genotypes 14 Real-TM Quant” (Sacace biotechnologies®, Italy) kit allowing for the detection of fourteen high-risk HPV genotypes: HPV 16, 31, 18, 39, 45, 59, 33, 35, 56, 68, 51, 52, 58 and 66. Results: the prevalence of high-risk HPV infections was 48.8% based on the appropriate PCR results (21/43). the most common HPV genotypes were HPV 39 (21.7%), HPV 35 (13.0%) and HPV 45 (13.0%). Two cases of multiple infections between HPV 39 - 45 and HPV 39 - 59 have been observed. HPV 16 was not detected in this study. Conclusions: We noted a high prevalence rate for HPV 39, HPV 35 and HPV 45, which are not covered by the commercial vaccines. We also found that the prevalence of HPV 18 was very low in this study and HPV 16 was not detected.

关键词： HPV Cancer CIN Cervix Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

dC-SIGN (Cd209) Promoter -336 A/G Polymorphism Is Not Associated with dengue Fever at Burkina Faso, West Africa

引用

Journal of Biosciences and Medicines 2023年第1期11卷 175-183页

作者： Fiffou Yougbare Aziz Sidi Aristide Tapsoba Pegdwendé Abel Sorgho Bagora Bayala Lassina Traore Prosper Bado Bapio Valérie Elvira Jean Télesphore Bazie Abdou Azaque Zoure Esther Mah Alima Traore théodora mahoukèdè zohoncon Sessi Frida Tovo Albert théophane Yonli Alice Kiba Florencia Wendkuuni djigma Jacques Simpore Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Université Saint thomas d’Aquin Ouagadougou Burkina Faso Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso Centre National de Transfusion Sanguine (CNTS) Ouagadougou Burkina Faso

Objective: the aim of this study was to characterize the polymorphisms of the dC-SIGN (-336 A/G, rs4804803) gene and their association with the immunopathogenicity of dengue fever in Burkina Faso. Methods: A total of three hundred forty-one subjects, patients of all ages have been included in the study: 208 persons presenting clinical signs of dengue fever which were confirmed by diagnostic and 133 Healthy Controls. Genotyping for the Cd209 variant (-336 A/G, rs4804803) was carried out using TaqMan SNP Genotyping Assays. Haplotype frequencies were inferred and compared between the study groups. Results: the percentage of men was 61.88% (211/341) and 38.12% (130/341) for women. the highest frequency of dengue fever (77.42%) was noted in patients with age between 20 to 40 years. Around 1.52% of the study population was positive for HIV, 40.55% were carriers of HBV and 3.83% of HCV. Genotype distribution of the Cd209 variant (-336 A/G, rs4804803) was in Hardy-Weinberg equilibrium in both patients and controls. the frequency of allele A was higher than allele G;however, statistical analyses showed that there is no significant difference in genotypes GG, AG and AA in patients and controls. Conclusion: this related no significant association with dengue for the variant of ?336 A/G in the dC-SIGN gene in an Ouagadougou population. However, our results offered the SNP frequencies in a West African population, which might be useful for the study of ethnic groups.

关键词： dengue Virus Cd209 Variant (-336 A/G rs4804803) Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

detection of Low-Risk and High-Risk Oncogenic Human Papillomavirus in Archived Tissues from ENT Tumors in Burkina Faso

引用

Journal of Biosciences and Medicines 2023年第9期11卷 171-180页

作者： Ali Kandé Maïmouna Ilboudo djénéba Ouermi Ina Marie Angèle Traore Abdoul Karim Ouattara théodora mahoukèdè zohoncon Esther Mah Alima Traore Florencia W. djigma dorcas Obiri-Yeboah Henriette Poaty Yvette Marie Chantal Gyebre Olga M. Lompo Charlemagne MR Ouedraogo Jacques Simpore Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Laboratoire de Biologie moléculaire et de génétique (LABIOGENE) UFR/SVT Université Joseph Ki-Zerbo Ouagadougou Burkina Faso University Post Office Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast Cape Coast Ghana Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Human papillomavirus (HPV) is classified into high-risk HPV (HR-HPV) and HPV (LR-HPV) according to their oncogenic potential. these viruses can be found in the cervix, vagina, vulva, anus and in the ENT sphere. HPV ENT infections can lead to benign or malignant tumors in which we could find both LR-HPV and HR-HPV genotypes. the objective of this study was to investigate the genotypes of HR-HPV and LR-HPV in archived tissue samples derived from both benign and malignant tumors of the ear, nose, and throat (ENT) in Ouagadougou, Burkina Faso. One hundred and twenty formalin-fixed, paraffin-embedded archived tissues of the ENT sphere from 26 benign tumors and 94 malignant tumors were included. the tissues were first deparaffinized with xylem. the extracted dNA was used to test for high-risk and low-risk HPV by Real-Time Multiplex PCR. HPV dNA was found in 57.7% (15/26) of benign tumors and 43.61% (41/94) of malignant tumors. the prevalence of HPV infection was 46.67% (56/120) in all tumors combined. the most common HPV genotypes found were HPV 11 (34.28%), HPV 6 (30%), HPV56 (14.28%) and HPV 33 (8.57%). there were 21.43% (12/56) cases of genotypes co-infections with 10 cases of double infection and 2 cases of triple infection. Both low-risk and high-risk HPV are found in ENT tumors with relatively high HPV prevalence.

关键词： HPV Archived Tissues ENT Tumor Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：