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Neonatal Morbidity and Mortality at Hospital Saint Camille de Ouagadougou (HOSCO): A Study from 2017 to 2020

Open Journal of Pediatrics 2024年第1期14卷 63-77页

作者： Nicaise Zagre Ines Kinda Abdoul Karim Ouattara Paul Ouedraogo théodora mahoukèdè zohoncon Caroline Yonaba Fla Koueta Jacques Simpore Hô pital Saint Camille de Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Joseph KI-ZERBO Ouagadougou Burkina Faso

Introduction: Neonatal pathology remains a real public health problem in developing countries. In Burkina Faso, this mortality has declined over the last ten years but remains below compared to the Sustainable development Goals, which is 12 per 1000 living births at most by 2030. this study aims to identify specific causes of neonatal morbidity and mortality and will contribute to the implementation of preventive and curative measures aimed at reducing neonatal mortality at HOSCO. Method: this was a retrospective study using the records and database of newborns hospitalized from January 1srt, 2017 to december 31srt, 2020. Using logistic regression, the factors associated with mortality were determined. Results: during the study period, 3020 newborns were hospitalized. Most newborns (83.71%) were referred by a peripheral health facility. the average age at admission was 0.3 days ± 0.9 and the sex ratio was 1.2. Prematurity was the leading cause of hospitalization (61.13%) followed by neonatal infection (38.34%) and neonatal suffering (23.88%). the mortality rate was 40.6% with 82.71% cases of death in the early neonatal period. the main causes of death were low birth weight (47.39%), respiratory distress (18.76%), neonatal suffering (17.37%) and neonatal infection (13.87%). Home delivery, gestational age 36 weeks, number of PNC 4, concept of resuscitation, Apgar at the 5th minute 7, birth weight 2000 g and >4000 g, respiratory distress, hypothermia, neurological disorders were factors associated with deaths. Conclusion: Neonatal mortality is influenced by both maternal and fetal factors and many of them are preventable.

关键词： Morbidity Mortality Newborns Neonatology HOSCO

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Cytotoxic Properties on Cervical and Liver Cancer Cells of Two Plant Recipes from Burkina Faso

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Journal of Biosciences and Medicines 2024年第6期12卷 216-229页

作者： Estelle Ouédraogo Bagora Bayala théodora mahoukèdè zohoncon Samiratou Ouédraogo Olga Mélanie Lompo Essi Etonam dovo Jean-Marie Compaoré Albert théophane Yonli Jacques Simpore Laboratory of Molecular Biology and Genetics (LABIOGENE) Joseph KI-ZERBO University Ouagadougou Burkina Faso Center of Biomolecular Research Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Ecole Normale Suprieure Koudougou Burkina Faso Hspital Saint Camille of Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculty of Health University Saint Thomas dAquin (USTA) Ouagadougou Burkina Faso Chair Research and Action against Cancer Department of Public Health Training and Research Unit in Health Sciences Joseph KI-ZERBO University Ouagadougou Burkina Faso National Population Health Observatory National Institute of Public Health Ouagadougou Burkina Faso the department of Epidemiology Biostatistics and Occupational Health School of Population and Global Health Montral Canada Laboratory of Pathological Anatomy and Cytology University Joseph KI-ZERBO Ouagadougou Burkina Faso TIIG LA TIIM Ouagadougou Burkina Faso

Cancer is one of the deadliest diseases in developing countries. In recent years, natural plant-based compounds have been used in the search for drugs to combat numerous diseases, including cancer. In this study, we evaluate the cytotoxic properties of paanfo tiben 1 and paanfo tiben 2, two traditional herbal formulations from Burkina Faso used in the treatment of cancer in Burkina Faso. To this end, the recipes were infused and freeze-dried. the dry extracts obtained were used to determine total phenolics and flavonoids content, assess antioxidant activity using the dPPH, ABTS and FRAP methods, evaluate anti-inflammatory properties by inhibiting 15-LOX, COX 1 and 2, and assess cytotoxic activity on HeLa cervical cancer and HePG2 liver cancer cell lines using the MTT test. the paanfo tiben 1 recipe showed the highest levels of total phenolics and flavonoids, as well as the best antioxidant activities, with IC50 values of 21.020 ± 0.6 µg/ml and 22.94 ± 0.57 µg/ml for dPPH and ABTS, and 165.15 mM EAA/mg dry extract for FRAP. It also exhibited the best cytotoxic activity with IC50 values of 112.02 ± 0.025 µg/ml on HeLa cells and 80.67 ± 6.08 µg/ml on HepG2 cells. On the other hand, paanfo tiben 2 exhibited the best anti-inflammatory activities through inhibition of 15-LOX and COX 1, with inhibition percentages at 100 µg/ml of 32.523% and 24.717 % respectively. these results could justify the traditional use of these two recipes by traditional health practitioners in the treatment of cancer sufferers in Burkina Faso.

关键词： Antioxidant Anti-Inflammatory Cytotoxicity Paanfo Tiben Burkina

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Molecular diagnosis of Sexual differentiation disorders in Burkina Faso

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Journal of Biosciences and Medicines 2024年第3期12卷 1-15页

作者： Bazie Bapio Valérie Elvira Jean Télesphore Sawadogo Soumaïla zohon.on.théodora mahoukèdè Tall Ahmadou Bado Prosper Soubeiga Serge théophile Ouattara Moussa Sorgho Abel Kiendrebeogo Touwendpoulimdé Isabelle Yonli Albert theophane djigma Florencia Millogo/Traore Timongo Françoise danielle Simpore Jacques Centre National de la Recherche Scientifique et Technologique (CNRST) Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso Université Joseph KI-ZERBO Unité de Formation en Sciences de la Vie et de la Terre Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigonie (CERBA) Ouagadougou Burkina Faso Centre Hospitalier Universitaire Régional de Ouahigouya Ouagadougou Burkina Faso Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Sex development anomalies represent a group of congenital pathologies in which chromosomal, gonadal and anatomical sex differentiation is atypical. the aim of our study was to use molecular biology techniques to elucidate sex in cases of anomalies of sexual differentiation in Burkina Faso. this cross-sectional study took place from March 2023 to June 2023. Oral and blood samples were collected respectively using sterile swabs and stored on Swab Kits and NUCLEIcardTM (https://***/product-ranges/nucleic-card/) were used to determine gonosome profiles. Extraction was carried out using the dNA Swap solution Kit and the dNA IQ System Kit, and a PowerPlex? 21 kit (Promega) for amplification. the Applied Biosystems 9700 thermal cycler was used for PCR followed by 36 cm capillary electrophoresis in the Applied Biosystems 3130 prism sequencer. Sequence files were analyzed using GeneMapper IdX v. 3.2 software. Seven (07) patients were registered during the study period. there were 4 cases of XX dSd or 46, XX dSd and 3 cases of XY dSd or 46, XY dSd. the median age of our patients was 16 years. Civilian sex was male in 4 cases and female in 3. the most frequent reason for consultation was micropenis in 3 cases, followed by primary amenorrhea and sex ambiguity. there were 03 cases of discrepancy between genetic sex and civil sex. the accessibility of molecular diagnosis is little known to clinicians. XX dSds or 46, XX dSds were the most frequent (4/7) in our study. the problem facing this situation is early diagnosis to help prevent complications in Burkina Faso.

关键词： Gonosome dSd Abnormal Sexual development Burkina Faso

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Mother-to-Child Transmission of Human Papillomavirus in Burkina Faso

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American Journal of Molecular Biology 2024年第1期14卷 13-24页

作者： Koudpoko Madeleine Kabre djénéba Ouermi théodora mahoukèdè zohoncon Fatié Porzé Wilfried Traore Prosper Bado Abdoul Karim Ouattara Ouamini Pulchérie de Prisca Gnoumou Rogomenoma Alice Ouedraogo Albert théophane Yonli Punya Akouélé Kuassi-Kpede Charlemagne Marie Ragnag-Néwendé Ouedraogo Jacques Simpore Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin Ouagadougou Burkina Faso Laboratoire de Biologie Moléculaire H&#244pital Saint Camille de Ouagadougou (HOSCO) Ouagadougou Burkina Faso University Joseph Ki-Zerbo UFR/SDS Ouagadougou Burkina Faso

Introduction: Human papillomavirus (HPV) infection is the most widespread sexually transmitted infection in the world. Today, there is growing evidence that HPV can be transmitted early in life, and one potential route is mother-to-child transmission. data on this route of HPV transmission are scarce in Africa and particularly in Burkina Faso, where no data on the subject are yet available. the aim of our study was to estimate the rate of mother-to-child transmission of HPV infection and to identify circulating genotypes. Methodology: Cervico-uterine samples were collected from 100 full-term pregnant women and, buccal samples were obtained from their newborns at Hopital Saint Camille de Ouagadougou (HOSCO) by the specialist physician. HPV dNA amplification and genotyping were performed by PCR followed by hybridization using the HPV direct Flow Chips kit, detecting 36 genotypes including 18 high-risk and 18 low-risk. Results: the prevalence of HPV in newborns was 8% (8/100). Six (6) HPV-positive neonates had HPV-positive mothers, while 2 HPV-positive neonates had HPV-negative mothers. the vertical transmission rate was 26.09% (6/23). Mother-newborn genotypes were concordant. However, the genotype profile of the newborns was more restricted than that of the mothers. Conclusion: HPV dNA was found in 8% of newborns in our study. the genotype profile of the mother-newborn pair was concordant. Asymptomatic HPV infection in a pregnant woman could constitute a risk factor for vertical transmission.

关键词： Mother-to-Child Transmission Human Papillomavirus PCR Genotypes Burkina Faso

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HPV/HBV or HPV/HCV Co-Infections in Women Treated for Chronic Hepatitis at Hôpital Saint Camille in Ouagadougou, Burkina Faso

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American Journal of Molecular Biology 2024年第1期14卷 1-12页

作者： Estelle Ouédraogo théodora mahoukèdè zohoncon Bagora Bayala Prosper Bado Rose P. Clémence da Rogomenoma Alice Ouedraogo Ina Marie Angèle Traoré Punya Akouélé Kuassi-Kpede Samiratou Ouédraogo Essi Etonam dovo Lassina Traoré Albert théophane Yonli Florencia Wendkuuni djigma Olga Mélanie Lompo Jacques Simpore Laboratory of Molecular Biology and Genetics (LABIOGENE) University Joseph KI-ZERBO Ouagadougou Burkina Faso Centre of Biomolecular Research Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Hô spital Saint Camille of Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculty of Health University Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Ecole Normale Supérieure Koudougou Burkina Faso Chair “Research and Action Against Cancer” Département de Santé Publique Training and Research Unit in Health Sciences University Joseph KI-ZERBO Ouagadougou Burkina Faso Observatoire National de la Santé de la Population Institut National de Santé Publique Ouagadougou Burkina Faso the department of Epidemiology Biostatistics and Occupational Health School of Population and Global Health Montreal Canada Laboratory of Pathological Anatomy and Cytology University Joseph KI-ZERBO Ouagadougou Burkina Faso

Introduction: Cervical cancer is a public health concern and is mainly caused by Human papillomaviruses (HPV). In many parts of the world, studies are being carried out to understand the different genotypes to better tackle this issue. We conducted a study to determine the prevalence of HPV genotypes in women with chronic hepatitis B or C infection, co-infected or not with HIV, treated at the Hôpital Saint Camille in Ouagadougou (Burkina Faso). Methods: this study was conducted from April to July 2023, including 100 women in gastroenterology at Hôpital Saint Camille. A questionnaire on their socio-demographic and life style was administrated;and endocervical samples were collected using sterile swabs and then sent to Centre of Biomolecular Research Pietro Annigoni (CERBA). HPV molecular detection and genotyping were performed by PCR and hybridization using the HPV direct Flow Chips kit. data were analysis using chi square test or Fischer’s exact test with a significance threshold for p Results: the prevalence of HPV infection was 28% (28/100) on the sample of women tested. the most frequent genotypes were HPV 52 (8.33%), followed by HPV 18 and 68 (6.25% each) for high-risk HPVs, and HPV 6, 44/55 and 62/81 (8.33% each) for low-risk HPVs. Conclusion: this study, the first of its kind in Burkina Faso on this group of the population, reveals that the most frequent genotypes found in this study are not included in the vaccine available in Burkina Faso (Gardasil®4).

关键词： Human Papillomavirus Cervical Cancer Hepatitis Human Immunodeficiency Virus Burkina Faso

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G6Pd deficiency and COVId-19 in Burkina Faso: A Possible Link?

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Journal of Biosciences and Medicines 2023年第1期11卷 57-69页

作者： Abdoul Karim Ouattara Lassina Traoré Tégwendé Rebeca Compaoré théodora mahoukèdè zohoncon Jacques Simporé Université Norbert Zongo/Centre Universitaire de Manga Koudougou Burkina Faso Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) UFR/SVT Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso département Biomédical et de Santé Publique Institut de Recherche en Sciences de la Santé (CNRST/IRSS) Ouagadougou Burkina Faso Faculté de Médecine Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso

Burkina Faso is a malaria-endemic country, with a high incidence of G6Pd deficiency (G6Pdd), which recorded its first case of COVId-19 in March 2020. G6Pdd leads to a decrease in the efficiency of erythrocytes to combat oxidative stress, while SARS-CoV-2 infection induces massive production of Reactive Oxygen Species (ROS) in patients. In the present review, we discuss a possible link between G6Pdd and SARS-CoV-2 infection. the mean prevalence of G6Pdd in Burkina Faso is estimated at 16.6% among males and 6.5% among females. A total of 21,128 cases of COVId-19 have been recorded in Burkina Faso with 387 deaths reported (with a mortality rate of 1.15% among diagnosed cases) as of August 30, 2022. To our knowledge, no association study between G6Pdd and SARS-CoV-2 infection has been conducted to date in Burkina Faso. However, several case reports around the world have described elevated risks of hemolysis and thrombosis, and other complications among G6Pd-deficient patients infected with SARS-CoV-2. the use of Hydroxychloroquine (HCQ) has also been deemed unsafe by some authors for the treatment of COVId-19 among patients with G6Pdd. Although HCQ has been shown to be well tolerated in COVId-19 patients in Burkina Faso, the drug could induce hemolytic crises in people with G6Pd deficiency. G6Pd is important in regulating ROS and maintaining erythrocyte homeostasis. In view of its high prevalence in Burkina Faso, determination of the G6Pd status is required in COVId-19 patients for adequate management such as identifying a subset of COVId-19 patients for whom close monitoring and supportive care may be essential and to restrict treatment with HCQ.

关键词： G6Pd deficiency COVId-19 CQ/HCQ Hemolysis Burkina Faso

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Clinical and Paraclinical Profiling of Patients with Viral Hepatitis B and C Attending Saint Camille Hospital in Ouagadougou (HOSCO)

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Journal of Biosciences and Medicines 2023年第10期11卷 225-240页

作者： théodora mahoukèdè zohoncon denise P. Ilboudo Lionel dahourou Titeon Rose Clémence Ido da Luc Zongo Jacques Simpore Laboratoire de Biologie Moléculaire H&ocircpital Saint Camille de Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculté des Sciences de la Santé (FSdS) Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Institut Supérieur du développement durable (ISdd) Université de Fada N’Gourma Fada N’Gourma Burkina Faso Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) UFR/SVT Université Joseph KI-ZERBO Ouagadougou Burkina Faso Unité de Formation et de Recherche en Sciences de la Santé (UFR/SdS) Université Saint Dominique d’Afrique de l’Ouest Kombissiri Burkina Faso

the present study aims to describe the clinical and paraclinical profile of patients infected by viral hepatitis B and C and follow-up. the clinical and paraclinical data used in this description are from patients infected by viral hepatitis B and C of the HOSCO Hepato-Gastroenterological department from May 15, 2021 to July 23, 2021. the informed consent was provided to each patient included in this study. “Univariate analyses were evaluated using Pearson’s Chi2 test” using R software version 4.0.2. during the study period, we identified 149 patients with viral hepatitis B and/or C who met our inclusion criteria. the sex ratio was 0.83 at the rate of 68 men for 81 women with the average age at 37.17 years ± 12.21 years. the most represented age group was 30 - 44 years (49.7%). the most incriminated risk factors were medical care by injection (62.58%), excision (31.90%), blood transfusion (4.29%) and scarification (1.23%). HBV infection was the majority with a frequency of 95.97%. the HBV viral load was measured in 91.95% of patients, 77.18% of whom had a detectable dNA viral load ≤ 2000 IU/mL. the clinical and biological course was good in patients after therapeutic initiation. HBV-HCV-HIV co-infection was 0.67%. Abdominal ultrasound was normal in 87.92% of patients. Fibrosis was minimal and moderate in 58.39% and 19.46% of patients. Among patients, 52.35% were on Tenofovir therapy, 2.68% on Sofosbuvir/Velpatasvir, 0.67% on ARVs and 44.29% did not require treatment. Viral hepatitis B and C are common, and both affect sex. thus, new screening strategies need to be implemented to improve the diagnosis of hepatitis B and C. Effective strategies against viral hepatitis B and C must be developed, subsequently.

关键词： Hepatitis B and C Co-Infection Primary Liver Cancer (PLC) Cirrhosis HOSCO

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Evolution of HBV Viral Load during Clinical and Biological Follow-Up of Chronic Hepatitis B Patients at the Saint Camille Hospital in Ouagadougou

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Advances in Infectious diseases 2023年第4期13卷 550-563页

作者： théodora mahoukèdè zohoncon T. Rose Clémence Ido da Nicaise Zagre Pauline Belemkoabga denise P. Ilboudo Abdoul Karim Ouattara Paul Ouedraogo Jacques Simpore department of Molecular Biology H&#244pital Saint Camille de Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculté de Médecine Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso

Hepatitis B virus (HBV) infection is a major public health problem worldwide. the aim of this study was to document the dynamics of HBV viral load during the follow-up of chronic hepatitis B patients at the Saint Camille Hospital in Ouagadougou (HOSCO) from 2017 to 2021. this descriptive retrospective study was carried out in the Hepato-Gastro-Enterology department of HOSCO and focused on patients who were undergoing treatment for chronic viral hepatitis B. A total of 260 cases of chronic hepatitis B were included in the study. the most affected age group was 21 to 30 years, accounting for 48.08% of the cases. Lifestyle factors included alcohol consumption (3.08%) and tobacco use (2.69%). Major risk factors for transmission included lack of vaccination (98.46%), family history of HBV infection (68.00%) and engagement in high-risk activities (28.00%). Patients requiring treatment were prescribed Tenofovir 300 mg tablets. FibroScan® showed the presence of stage F3-F4 fibrosis (2.14%) and S3 steatosis (13.33%). After one year of follow-up, 6.92% of patients achieved an undetectable viral load with normalized transaminase levels. the majority of other patients had a detectable viral load but below 20,000 IU/mL. the prevalence of viral hepatitis B remains significant worldwide. Although effective and well-monitored treatment can lead to undetectable viremia, prevention remains the most effective strategy for successful management of this disease.

关键词： Chronic Viral Hepatitis B Viral dNA Follow-Up Evolution of Viral Load

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detection of Human Papillomavirus dNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso

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American Journal of Molecular Biology 2023年第4期13卷 276-290页

作者： théodora mahoukèdè zohoncon Shoukrat Ohuwa Toyin Bello Prosper Bado Rogomenoma Alice Ouédraogo Estelle Ouédraogo Ina Marie Angèle Traoré Abdoul Karim Ouattara Florencia Wenkunni djigma Albert théophane Yonli Assita Sanou-Lamien Olga Mélanie Lompo Jacques Simpore Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Centre Hospitalo-Universitaire Yalgado Ouedraogo Ouagadougou Burkina Faso Pathological Anatomy and Cytology Laboratory Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Introduction: Cervical cancer, caused by persistent high-risk human papillomavirus (HPV) infection, remains a global public health problem. the cellular transformation and maintenance of the malignant phenotype of these HPVs are attributed to the viral oncoproteins E6 and E7. Objective: this study aims to detect the presence of human papillomavirus dNA and E6/E7 oncoprotein mRNA of HPV genotypes 16, 18, 31 and 33 in cases of cervical cancer and precancerous lesions, histologically confirmed in Burkina Faso. Methods: this descriptive cross-sectional study focused on cases of cervical cancer and high-grade intraepithelial neoplasia (CIN) and was conducted from June to december 2022. One hundred (100) samples of fixed and paraffin-embedded tissues were collected from the pathological anatomy and cytology laboratories of hospitals in the capital of Burkina Faso. High-risk human papillomavirus (HR-HPV) dNA was detected using multiplex real-time PCR, while the presence of E6 and E7 mRNA in cervical cancer and high-grade CIN samples was determined using real-time Reverse Transcriptase-PCR (RT-PCR) with TaqMan probes. Results: the mean age of women diagnosed with cervical cancer and high-grade CIN was 50.81 ± 13.65 years, ranging from 22 to 82 years. Cervical cancer and high-grade CIN were positive for at least one high-risk human papillomavirus (HR-HPV) in 80% of cases. the most prevalent genotypes observed were HPV16, 18, 31, and 33, collectively accounting for 70.08% of cases. Of the 89 samples that tested positive for HR-HPV genotypes 16, 18, 31, and 33, 88 (98.88%;95% CI: [94.58 - 99.94]) were also positive for the presence of mRNA encoding the E6 and E7 oncoproteins of HPV16, 18, 31, and 33. Conclusion: In the presence of HPV dNA, testing for E6 and E7 oncoprotein mRNA could serve as a promising biomarker and valuable tool for improved assessment of the progression to cervical cancer.

关键词： HPV E6/E7 Cervical Cancer Precancerous Lesions Burkina Faso

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dC-SIGN (Cd209) Promoter -336 A/G Polymorphism Is Not Associated with dengue Fever at Burkina Faso, West Africa

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Journal of Biosciences and Medicines 2023年第1期11卷 175-183页

作者： Fiffou Yougbare Aziz Sidi Aristide Tapsoba Pegdwendé Abel Sorgho Bagora Bayala Lassina Traore Prosper Bado Bapio Valérie Elvira Jean Télesphore Bazie Abdou Azaque Zoure Esther Mah Alima Traore théodora mahoukèdè zohoncon Sessi Frida Tovo Albert théophane Yonli Alice Kiba Florencia Wendkuuni djigma Jacques Simpore Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Université Saint thomas d’Aquin Ouagadougou Burkina Faso Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso Centre National de Transfusion Sanguine (CNTS) Ouagadougou Burkina Faso

Objective: the aim of this study was to characterize the polymorphisms of the dC-SIGN (-336 A/G, rs4804803) gene and their association with the immunopathogenicity of dengue fever in Burkina Faso. Methods: A total of three hundred forty-one subjects, patients of all ages have been included in the study: 208 persons presenting clinical signs of dengue fever which were confirmed by diagnostic and 133 Healthy Controls. Genotyping for the Cd209 variant (-336 A/G, rs4804803) was carried out using TaqMan SNP Genotyping Assays. Haplotype frequencies were inferred and compared between the study groups. Results: the percentage of men was 61.88% (211/341) and 38.12% (130/341) for women. the highest frequency of dengue fever (77.42%) was noted in patients with age between 20 to 40 years. Around 1.52% of the study population was positive for HIV, 40.55% were carriers of HBV and 3.83% of HCV. Genotype distribution of the Cd209 variant (-336 A/G, rs4804803) was in Hardy-Weinberg equilibrium in both patients and controls. the frequency of allele A was higher than allele G;however, statistical analyses showed that there is no significant difference in genotypes GG, AG and AA in patients and controls. Conclusion: this related no significant association with dengue for the variant of ?336 A/G in the dC-SIGN gene in an Ouagadougou population. However, our results offered the SNP frequencies in a West African population, which might be useful for the study of ethnic groups.

关键词： dengue Virus Cd209 Variant (-336 A/G rs4804803) Burkina Faso

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