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Visualizing the crystallization of sodium chloride under supersaturated condition

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Nano Research 2024年第8期17卷 7786-7792页

作者： Mao Ye Tao Xu Yuwei Xiong Yatong Zhu Mingrui Zhou Longxiang han Jinyi sun Ming Qin Litao sun SEU-FEI Nano-Pico Center Key Laboratory of MEMS of Ministry of EducationSchool of Integrated CircuitsSoutheast UniversityNanjing 210096China

Crystallization in supersaturated solution plays a fundamental role in a variety of natural and industrial ***,a thorough understanding of crystallization phenomena in supersaturated solution is still difficult because the real-time visualization of crystallization processes under supersaturated condition is a great ***,an electron beam-induced crystallization method was carried out in in situ liquid cell transmission electron microscopy(TEM)to visualize the crystallization of NaCl under supersaturated condition in real *** steps and behaviors in the crystallization of NaCl were captured and clarified,including the growth of NaCl nanocrystals with different morphologies,the formation of initial crystalline seeds from amorphous ion clusters,and the non-equilibrium growth behaviors caused by uneven distribution of precursor *** study provides the real-time visualization of detailed nucleation and growth behaviors in NaCl crystallization and brings an ideal strategy for investigating crystallization phenomena under supersaturated condition.

关键词： crystallization in situ liquid cell transmission electron microscopy(TEM) electron-water interaction sodium chloride

Improve meat production and virus resistance by simultaneously editing multiple genes in livestock using Cas12i^(Max)

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Science China(Life Sciences) 2024年第3期67卷 555-564页

作者： Jilong Ren Tang Hai Yangcan Chen Ke sun Zhiqiang han Jing Wang Chongyang Li Qingwei Wang Leyun Wang Huabing Zhu Dawei Yu wei Li Shanjiang Zhao State Key Laboratory of Animal Nutrition Key Laboratory of Animal GeneticsBreeding and Reproduction of Ministry of Agriculture and Rural AffairsInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratoryf Stem Cell and Reproductive Biology Instituteof ZooogyChinese Academyof SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China Beijing Farm Animal Research Center Institute of ZoologyChinese Academy of SciencesBeijing 100101China

The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated gene(Cas) system is continually optimized to achieve the most efficient gene editing effect. The Cas12i^(Max), a Cas12i variant, exhibits powerful DNA editing activity and enriches the gene editing toolbox. However, the application of Cas12i^(Max)in large domestic animals has not yet been reported. To verify the efficiency and feasibility of multiple gene editing in large animals, we generated porcine fibroblasts with simultaneous knockouts of IGF2, ANPEP, CD163,and MSTN via Cas12i^(Max)in one step. Phenotypically stable pigs were created through somatic cell nuclear transfer technology. They exhibited improved growth performance and muscle quality. Furthermore, we simultaneously edited three genes in bovine fibroblasts. A knockout of MSTN and PRNP was created and the amino acid Q-G in CD18 was precisely substituted. Meanwhile, no off-target phenomenon was observed by sum-type analysis or off-target detection. These results verified the effectiveness of Cas12i^(Max)for gene editing in livestock animals and demonstrated the potential application of Cas12i^(Max)in the field of animal trait improvement for agricultural production.

关键词： Cas12i^(Max) gene editing livestock multiple trait improvement

Saturation Estimation with Complex Electrical Conductivity for Hydrate-Bearing Clayey Sediments:An Experimental Study

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Journal of Ocean University of China 2024年第1期23卷 173-189页

作者： XING Lanchang ZhanG Shuli ZhanG Huanhuan WU Chenyutong WANG Bin LAO Liyun wei wei han weifeng wei Zhoutuo GE Xinmin DENG Shaogui College of Control Science and Engineering China University of Petroleum(East China)Qingdao 266580China School of Water Energy and EnvironmentCranfield UniversityCranfield MK430ALUnited Kingdom Department of Alternative Energy PetroChina Research Institute of Petroleum Exploration&DevelopmentLangfang 065007China School of Geosciences China University of Petroleum(East China)Qingdao 266580China Laboratory for Marine Mineral Resources Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

Clays have considerable influence on the electrical properties of hydrate-bearing *** is desirable to understand the electrical properties of hydrate-bearing clayey sediments and to build hydrate saturation(S_(h))models for reservoir evaluation and *** electrical properties of tetrahydrofuran-hydrate-bearing sediments with montmorillonite are characterized by complex conductivity at frequencies from 0.01 Hz to 1 *** effects of clay and Sh on the complex conductivity were analyzed.A decrease and increase in electrical conductance result from the clay-swelling-induced blockage and ion migration in the electrical double layer(EDL),*** quadrature conductivity increases with the clay content up to 10%because of the increased surface site density of counterions in *** the in-phase conductivity and quadrature conductivity decrease consistently with increasing Sh from 0.50 to *** sets of models for Sh evaluation were *** model based on the Simandoux equation outperforms Archie’s formula,with a root-mean-square error(E_(RMS))of 1.8%and 3.9%,respectively,highlighting the clay effects on the in-phase *** fre-quency effect correlations based on in-phase and quadrature conductivities exhibit inferior performance(E_(RMS)=11.6%and 13.2%,re-spectively)due to the challenge of choosing an appropriate pair of frequencies and intrinsic uncertainties from two *** second-order Cole-Cole formula can be used to fit the complex-conductivity *** pair of inverted Cole-Cole parameters,i.e.,characteristic time and chargeability,is employed to predict S_(h) with an E_(RMS) of 5.05%and 9.05%,respectively.

关键词： gas hydrate complex electrical conductivity hydrate-bearing clayey sediment hydrate saturation Simandoux equation frequency dispersion Cole-Cole formula

Polymeric viologen-based electron transfer mediator for improving the photoelectrochemical water splitting on Sb_(2)Se_(3) photocathode

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Fundamental Research 2024年第2期4卷 291-299页

作者： Chang Liu Fusheng Li Linqin Wang Zeju Li Yilong Zhao Yingzheng Li Wenlong Li ziqi Zhao Ke Fan Fei Li Licheng sun State Key Laboratory of Fine Chemicals Institute of Artificial PhotosynthesisDUT-KTH Joint Education and Research Centre on Molecular DevicesInstitute for Energy Science and TechnologyDalian University of TechnologyDalian 116024China Center of Artificial Photosynthesis for Solar Fuels School of ScienceWestlake UniversityHangzhou 310024China Department of Chemistry School of Engineering Sciences in ChemistryBiotechnology and HealthKTH Royal Institute of TechnologyStockholm 10044Sweden

The photogenerated charge carrier separation and transportation of inside photocathodes can greatly influence the performance of photoelectrochemical(PEC)H2 production *** TiO_(2) with p-type semiconductors to construct heterojunction structures is one of the most widely used strategies to facilitate charge separation and ***,the band position of TiO_(2) could not perfectly match with all p-type ***,taking antimony selenide(Sb_(2)Se_(3))as an example,a rational strategy was developed by introducing a viologen electron transfer mediator(ETM)containing polymeric film(poly-1,1′-dially-[4,4′-bipyridine]-1,1′-diium,denoted as PV^(2+))at the interface between Sb_(2)Se_(3) and TiO_(2) to regulate the energy band alignment,which could inhibit the recombination of photogenerated charge carriers of *** Pt as a catalyst,the constructed Sb_(2)Se_(3)/PV^(2+)/TiO_(2)/Pt photocathode showed a superior PEC hydrogen generation activity with a photocurrent density of−18.6 mA cm^(-2) vs.a reversible hydrogen electrode(RHE)and a half-cell solar-to-hydrogen efficiency(HC-STH)of 1.54%at 0.17 V ***,which was much better than that of the related Sb_(2)Se_(3)/TiO_(2)/Pt photocathode without PV^(2+)(−9.8 mA cm^(-2),0.51%at 0.10 V ***).

关键词： Viologen Electron transfer mediator Sb2Se3 photocathode Solar water splitting Hydrogen evolution reaction

A data-driven approach to RUL prediction of tools

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Advances in Manufacturing 2024年第1期12卷 6-18页

作者： wei Li Liang-Chi Zhang Chu-han Wu Yan Wang Zhen-Xiang Cui Chao Niu Department of Mechanical Engineering University College LondonLondonWC1E 7JEUK Shenzhen Key Laboratory of Cross-Scale Manufacturing Mechanics Southern University of Science and TechnologyShenzhen518055GuangdongPeople’s Republic of China SUSTech Institute for Manufacturing Innovation Southern University of Science and TechnologyShenzhen518055GuangdongPeople’s Republic of China Department of Mechanics and Aerospace Engineering Southern University of Science and TechnologyShenzhen518055GuangdongPeople’s Republic of China School of Mechanical and Manufacturing Engineering The University of New South WalesKensingtonNSW2052Australia Baoshan Iron&Steel Co. Ltd.Shanghai200941People’s Republic of China

An effective and reliable prediction of the remaining useful life(RUL)of a tool is important to a metal forming process because it can significantly reduce unexpected maintenance,avoid machine shutdowns and increase system *** study proposes a new data-driven approach to the RUL prediction for metal forming processes under multiple contact sliding *** data-driven approach took advantage of bidirectional long short-term memory(BLSTM)and convolutional neural networks(CNN).A pre-trained lightweight CNN-based network,WearNet,was re-trained to classify the wear states of workpiece surfaces with a high accuracy,then the classification results were passed into a BLSTM-based regression model as inputs for RUL *** experimental results demonstrated that this approach was able to predict the RUL values with a small error(below 5%)and a low root mean square error(RMSE)(around 1.5),which was more superior and robust than the other state-of-the-art methods.

关键词： Remaining useful life(RUL) Bidirectional long short-term memory(BLSTM) Data-driven approach Metal forming

Synergistic Effect of Nitrogen/Phosphorus Co-Doping and Molybdenum Carbide Induced Electron Redistribution of Carbon Layer to Boost Hydrogen Evolution Reaction

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Chinese Journal of Chemistry 2024年第1期42卷 55-60页

作者： Jiamin Xiao Peng Li Yanyan sun Heng Liu Javid Khan handong Zhang han Zhou Yaqiong Su Shuangyin Wang Lei han College of Materials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean EnergyHunan UniversityChangshaHunan 410082China School of Chemistry Engineering Research Center of Energy Storage Materials and Devices Ministry of EducationXi'an Key Laboratory of Sustainable Energy Materials ChemistryState Key Laboratory of Electrical Insulation and Power EquipmentXi'an Jiaotong UniversityXi'anShaanxi 710049China School of Materials Science and Engineering Central South UniversityChangshaHunan 410083China state Key Laboratory of Chem/Bio-Sensingand Chemometrics College of Chemistry and Chemical EngineeringHunan University ChangshaHunan 410082China Laboratory of Inorganic Materials and Catalysis Department of Chemical Engineering and ChemistryEindhoven University of Technology P.O.Box5135600 MB EindhovenThe Netherlands

The development of highly efficient non-precious-metal-based electrocatalysts for the hydrogen evolution reaction is imperative for promoting the large-scale application of electrochemical water ***,nitrogen/phosphorus co-doped carbon nanorods encapsulated Mo_(2)C nanoparticles(Mo_(2)C@PNc)have been prepared by pre-phosphating treatment in combination of the coordination with polydopamine and the subsequent *** phosphating temperature has a significant effect on the content of phosphorus within the resultant Mo_(2)C@PNC,and the optimal catalyst delivers superior HER activity with the low overpotential of 104 mV at a current density of 10 mAcm^(-2) and good stability for 8 h,which has been theoretically demonstrated to originate from the synergistic effect between P doping and Mo_(2)C induced electron redistribution of nitrogen-doped carbon layer.

关键词： Molybdenum carbide N/P co-doped carbon Hydrogen evolution Synergistic effect Electron redistribution Comprehensive

Broadening environmental research in the era of accurate protein structure determination and predictions

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Frontiers of Environmental Science & Engineering 2024年第7期18卷 169-178页

作者： Mingda Zhou Tong Wang Ke Xu han Wang Zibin Li wei-xian Zhang Yayi Wang State Key Laboratory of Pollution Control and Resources Reuse Shanghai Institute of Pollution Control and Ecological SecurityCollege of Environmental Science and EngineeringTongji UniversityShanghai 200092China Translational Research Institute of Brain and Brain-Like Intelligence Shanghai Fourth People's HospitalSchool of MedicineTongji UniversityShanghai 200434China

The deep-learning protein structure prediction method AlphaFold2 has garnered enormous attention beyond the realm of structural biology,for its groundbreaking contribution to solving the"protein foiding problem"In this perspective,we explore the connection between protein structure studies and environmental research,delving into the potential for addressing specific environmental *** are promising for environmental applications because of the functional diversity endowed by their structural ***,structural studies on proteins with environmental significance remain ***,we present the opportunity to study proteins by advancing experimental determination and deep-learning prediction ***,the latest progress in environmental research via cryogenic electron microscopy is *** allows us to determine the structure of protein complexes in their native state within cells at molecular resolution,revealing environmentally-associated structural *** the remarkable advancements in computational power and experimental resolution,the study of protein structure and dynamics has reached unprecedented depth and *** advancements will undoubtedly accelerate the establishment of comprehensive environmental protein structural and functional *** opportunities for protein engineering exist to enable innovative solutions for environmental applications,such as the degradation of persistent contaminants,and the recovery of valuable metals as well as rare earth elements.

关键词： Environmental proteins Protein structure Cryogenic electron microscopy Protein structure prediction Protein engineering Artificial Intelligence

Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

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Journal of Integrative Agriculture 2024年第5期23卷 1494-1506页

作者： Xiaohui Wu Mengyuan Zhang Zheng Zheng ziqi sun Feiyan Qi Hua Liu Juan Wang Mengmeng Wang Ruifang Zhao Yue Wu Xiao Wang Hongfei Liu Wenzhao Dong Xinyou Zhang College of Agronomy Henan Agricultural UniversityZhengzhou 450046China Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop ImprovementZhengzhou 450002China The Shennong Laboratory Zhengzhou 450002China Shangqiu Academy of Agricultural and Forestry Sciences Shangqiu 476000China

Peanut(Arachis hypogaea L.)is a globally important oil *** blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses *** web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web *** the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch *** on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome *** region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two *** markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.

关键词： peanut web blotch bulked segregant analysis KAsP markers resistant gene

Precise Planar-Twisted Molecular Engineering to Construct Semiconducting Polymers with Balanced Absorption and Quantum Yield for Efficient Phototheranostics

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Research 2024年第2期2023卷 227-238页

作者： Xiang Su Zhirong Bao wei Xie Deliang Wang Ting han Dong Wang Ben Zhong Tang Center for AIE Research Shenzhen Key Laboratory of Polymer Science and TechnologyGuangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Materials Science and EngineeringShenzhen UniversityShenzhen 518060China School of Biomedical and Pharmaceutical Sciences Guangdong University of TechnologyGuangzhou 510006China Department of Radiation and Medical Oncology Hubei Key Laboratory of Tumor Biological BehaviorsHubei Cancer Clinical Study CenterZhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Materials Chemistry Huzhou UniversityHuzhou 313000China School of Science and Engineering Shenzhen Institute of Aggregate Science and TechnologyThe Chinese University of Hong KongShenzhenGuangdong 518172China

Semiconducting polymers(SPs)have shown great feasibility as candidates for near-infrared-II(NIR-Ⅱ)fluorescence imaging-navigated photothermal therapy due to their strong light-harvesting ability and flexible ***,the fluorescence signal of traditional SPs tends to quench in their aggregate states owing to the strongπ-πstacking,which can lead to the radiative decay pathway shutting *** address this issue,aggregation-induced emission effect has been used as a rational tactic to boost the aggregate-state fluorescence of NIR-Ⅱ*** this contribution,we developed a precise molecular engineering tactic based on the block copolymerizations that integrate planar and twisted segments into one conjugated polymer backbone,providing great flexibility in tuning the photophysical properties and photothermal conversion capacity of *** monomers featured with twisted and planar architectures,respectively,were tactfully incorporated via a ternary copolymerization approach to produce a series of new *** optimal copolymer(SP2)synchronously shows desirable absorption ability and good NIR-Ⅱquantum yield on the premise of maintaining typical aggregation-induced emission characteristics,resulting in balanced NIR-Ⅱfluorescence brightness and photothermal ***-dispersible nanoparticles fabricated from the optimal SP2 show efficient photothermal therapeutic effects both in vitro and in *** in vivo investigation reveals the distinguished NIR-Ⅱfluorescence imaging performance of SP2 nanoparticles and their photothermal ablation toward tumor with prominent tumor accumulation ability and excellent biocompatibility.

关键词： property conducting twisted