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REGγ drives Lgr5^(+) stem cells to potentiate radiation induced intestinal regeneration

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Science China(Life Sciences) 2022年第8期65卷 1608-1623页

作者： Xiangzhan Zhu Minglei yang Zaijun Lin Solomon Kibreab Mael Ya Li Lili Zhang Yaqi Kong Yaodong Zhang Yuping Ren Jianhui Li zimeng Wang Ying Zhang Bo yang Tingmei Huang Fangxia Guan Zhenlong Li Robb E Moses Lei Li Bing Wang Xiaotao Li Bianhong Zhang Changning Maternity and Infant Health Hospital East China Normal UniversityShanghai 20051China Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical SciencesSchool of Life SciencesEast China Normal UniversityShanghai 200241China Department of Orthopedic Oncology Changzheng Hospitalthe Second Military Medical UniversityShanghai 200003China Department of Spinal Surgery Shidong HospitalYangpu DistrictShidong Hospital Affiliated to University of Shanghai for Science and TechnologyShanghai 200438China Henan Key Laboratory for Helicobacter pylori&Microbiota and GI cancer Marshall Medical Research CenterThe Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Neonatology Children’s Hospital Affiliated to Zhengzhou UniversityZhengzhou 450018China Department of Pathology Xuchang Central Hospital Affiliated to Henan University of Science and TechnologyXuchang 461000China School of Life Sciences Zhengzhou UniversityZhengzhou 450001China Department of Hematology&Hematopoietic Cell Transplantation City of Hope National Medical CenterLos Angeles CA 91010USA Department of Molecular and Cellular Biology Baylor College of MedicineHouston TX 77030USA Department of Oncological Surgery Minhang BranchShanghai Cancer CenterFudan UniversityShanghai 200240China

Leucine-rich repeat containing G protein-coupled receptor 5(Lgr5), a marker of intestinal stem cells(ISCs), is considered to play key roles in tissue homoeostasis and regeneration after acute radiation injury. However, the activation of Lgr5 by integrated signaling pathways upon radiation remains poorly understood. Here, we show that irradiation of mice with whole-body depletion or conditional ablation of REGγ in Lgr5^(+) stem cell impairs proliferation of intestinal crypts, delaying regeneration of intestine epithelial cells. Mechanistically, REGγ enhances transcriptional activation of Lgr5 via the potentiation of both Wnt and Hippo signal pathways. TEAD4 alone or cooperates with TCF4, a transcription factor mediating Wnt signaling, to enhance the expression of Lgr5. Silencing TEAD4 drastically attenuated β-catenin/TCF4 dependent expression of Lgr5. Together, our study reveals how REGγ controls Lgr5 expression and expansion of Lgr5+stem cells in the regeneration of intestinal epithelial cells.Thus, REGγ proteasome appears to be a potential therapeutic target for radiation-induced gastrointestinal disorders.

关键词： REGγ intestinal stem cell Lgr5 Wnt YAP

Transcriptomic analysis identifies a pan-cancer association of IL27 expression with cancer prognosis and immune microenvironment

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Genes & Diseases 2023年第2期10卷 393-395页

作者： Kaili Liao Jingyi Wang zimeng Li Yuqiu Li Yunqi Cheng Yingcheng He Hongping Wan Xiaozhong Wang Department of Clinical Laboratory The Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi 330006China School of Public Health Nanchang UniversityNanchangJiangxi 330006China Department of Pathology The First Affiliated Hospital of Nanchang UniversityNanchangJiangxi 330006China

Nowadays great progress has been made in targeted therapy and comprehensive therapy for a few cancers. But the prognosis of the vast majority of cancer patients is still weakness. It has been proven that inflammation can affect the development and treatment of cancer. Some previous studies have suggested that inflammation-related interleukins (ILs) play a significant role in the development of some cancers.1,2 ILs act as a considerable role in immunity,3 and inflammation affects the development of cancer. Therefore, the expression profile of IL gene may also be one of the vital markers of early pathological changes, occurrence and progression of cancer.4,5 Currently, studies on the expression of IL27 in cancer are still extremely limited. Our work comprehensively and systematically studied the IL27 gene expression in the generic cancer when compared with the existing IL27 gene related research experiment. We screened out cancer species linked with poor prognosis, and explored the correlation between IL27 gene expression and immune cell infiltration and immune microenvironment in these cancers further, providing an increasing number of the direct basis for subsequent studies. Our findings showed that IL27 can be used to assess prognosis and immune infiltration in patients with colon adenocarcinoma (COAD), glioblastoma multiforme (GBM), head and neck squamous cell carcinoma (HNSC), skin cutaneous melanoma (SKCM) and testicular germ cell tumors (TGCT) as a biomarker.

关键词： IL27 inflammation prognosis

巴西诺卡菌感染所致的原发性皮肤诺卡菌病一例

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中国麻风皮肤病杂志 2024年第4期40卷 283-285页

作者：庄凯文冉昕游紫梦孙计同冉玉平四川大学华西医院皮肤科四川成都610041

原发性皮肤诺卡菌病是一种由诺卡菌属直接感染皮肤及皮下组织引起的少见感染性疾病,临床表现多样,极易被误诊。本例患者左前臂红斑、结节、脓肿及溃疡,表面大量脓性分泌物2周。活检组织培养长出黄白色菌落,鉴定为巴西诺卡菌。患者口服... 详细信息

原发性皮肤诺卡菌病是一种由诺卡菌属直接感染皮肤及皮下组织引起的少见感染性疾病,临床表现多样,极易被误诊。本例患者左前臂红斑、结节、脓肿及溃疡,表面大量脓性分泌物2周。活检组织培养长出黄白色菌落,鉴定为巴西诺卡菌。患者口服复方磺胺甲噁唑治疗50天后痊愈。

关键词：原发性皮肤诺卡菌病巴西诺卡菌复方磺胺甲噁唑

3D hypothesis clustering for cross-view matching in multiperson motion capture

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Computational Visual Media 2020年第2期6卷 147-156页

作者： Miaopeng Li zimeng Zhou Xinguo Liu State Key Lab of CAD&CG Zhejiang UniversityHangzhou310058China

We present a multiview method for markerless motion capture of multiple people. The main challenge in this problem is to determine crossview correspondences for the 2 D joints in the presence of noise. We propose a 3 D hypothesis clustering technique to solve this problem. The core idea is to transform joint matching in 2 D space into a clustering problem in a 3 D hypothesis space. In this way, evidence from photometric appearance, multiview geometry, and bone length can be integrated to solve the clustering problem efficiently and robustly. Each cluster encodes a set of matched 2 D joints for the same person across different views, from which the 3 D joints can be effectively inferred. We then assemble the inferred 3 D joints to form full-body skeletons for all persons in a bottom–up way. Our experiments demonstrate the robustness of our approach even in challenging cases with heavy occlusion,closely interacting people, and few cameras. We have evaluated our method on many datasets, and our results show that it has significantly lower estimation errors than many state-of-the-art methods.

关键词： multi-person motion capture cross-view matching clustering human pose estimation

中药漱口液对气管插管患者呼吸机相关性肺炎影响的meta分析

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国际医药卫生导报 2024年第4期30卷 590-596页

作者：魏婷妤蒋玮婷李紫梦蒋雅昕陈碧贞福建中医药大学护理学院福州350122 福建中医药大学附属第二人民医院医院感染管理处福州350003

目的基于meta分析评价中药漱口液对气管插管患者呼吸机相关性肺炎(VAP)的预防效果。方法系统检索中国知网数据库(CNKI)、万方数据库(Wanfang Data)、维普数据库(VIP)、中国生物医学文献数据库(CBM)、PubMed、Embase、Cochrane Library、... 详细信息

目的基于meta分析评价中药漱口液对气管插管患者呼吸机相关性肺炎(VAP)的预防效果。方法系统检索中国知网数据库(CNKI)、万方数据库(Wanfang Data)、维普数据库(VIP)、中国生物医学文献数据库(CBM)、PubMed、Embase、Cochrane Library、Web of Science,检索时间为建库至2023年8月,收集中药漱口液预防VAP的随机对照试验,同时追溯相关文献的参考文献,2名研究者独立筛选文献,对纳入的文献进行文献质量评价和偏倚风险评估,提取资料后采用RevMan 5.4软件进行meta分析。结果最终纳入14篇文献,中文9篇、英文5篇,共计1298例患者,其中试验组(中药漱口液)649例、对照组(氯己定含漱液)649例。meta分析结果显示,试验组VAP发生率[RR=0.48,95%CI(0.39,0.60),P<0.00001]、真菌感染发生率[RR=0.28,95%CI(0.16,0.48),P<0.00001]、口腔溃疡发生率[RR=0.31,95%CI(0.20,0.49),P<0.00001]、口腔异味发生率[RR=0.36,95%CI(0.26,0.50),P<0.00001]和机械通气天数[SMD=-1.55,95%CI(-2.88,-0.21),P=0.02]均低于对照组,差异均有统计学意义,而两组在改善临床肺部感染评分(CPIS)方面差异无统计学意义[MD=-0.04,95%CI(-0.59,0.50),P=0.87]。结论中药漱口液相比于氯己定含漱液更能降低VAP发生率,减少真菌感染、口腔溃疡、口腔异味的发生和缩短机械通气天数,但在降低CPIS方面仍有待进一步研究,需要更多大样本、高质量的随机对照试验来验证中药漱口液对VAP的预防效果。

关键词：呼吸机相关性肺炎防控中药漱口液 meta分析

Effect of Dangfei Liganning capsule(当飞利肝宁胶囊) on liver X receptor α/steroid regulatory element binding protein-1/fatty acid synthase signal pathway in rats with metabolic-associated fatty liver disease

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Journal of Traditional Chinese Medicine 2022年第6期42卷 940-947页

作者： LI Xiaoling SUN Fengxia SHANG zimeng ZHANG Yingxue LI Jie ZHANG Qiuxiang Infections Department Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing 100010China Infections Department Beijing Hospital of Traditional Chinese Medicine Shunyi BranchBeijing 101300China

OBJECTIVE: To study the mechanism of Dangfei Liganning capsule(当飞利肝宁胶囊) in the treatment of rats with metabolic associated fatty liver disease(MAFLD). METHODS: Totally 48 specific pathogen free SpragueDawley male rats were randomly divided into normal Group, model group, Dangfei Liganning high, moderate, and low-dose groups and Essentiale group which were fed with high fat diet for 8 weeks, and gavage and molding were carried out simultaneously. Dangfei Liganning high, middle and low-dose group were given 0.27, 0.135 and 0.0675 g·kg-1·d-1 respectively by gavage, Essentiale group was given 0.123 g·kg-1·d-1 by gavage, the same amount of distilled water was given by gavage in the normal group and the model group. The rats were weighed at the 0th week, 2nd week, 4th week, 6th week and 8th weekend respectively. The rats were sacrificed at the end of the 8th week. Serum levels of alanine aminotransferase(ALT), alanine aminotransferase(AST),triglyceride(TG), total cholesterol(CHO), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein (LDL-C), total protein(TP), albumin(Alb), globulin(GLB), total bilirubin(TBIL), direct bilirubin(DBIL), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were measured. The levels of liver tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and liver pathology [hematoxylin and eosin(HE) staining, oil red O staining] were detected. The expression levels of liver X receptor α(LXRα), steroid regulatory element binding protein-1(SREBP-1) and fatty acid synthase(FAS) were detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction reverse transcription-polymerase chain reaction. RESULTS: From the beginning to the 8th week, the growth rate of body weight in the Dangfei Liganning highdose group was slower than all other groups. There was no significant difference in ALB level in all groups(P > 0.05). Compared with the model group, the levels of ALT, AST, LDL-C, TG, CHO, TP, GLB, TBIL, DBIL, IL-6, TNF-α were significantly decreased and HDL

关键词： metabolic associated fatty liver disease liver X receptors steroid regulatory element binding protein-1 fatty acid synthases signal transduction Dangfei Liganning capsule

去应力退火对LPBF TC4合金在1%NaCl溶液中的腐蚀行为影响

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现代交通与冶金材料 2024年第4期4卷 80-86页

作者：肖梓萌韩剑阳王泽鑫夏春智周慧玲江苏科技大学材料科学与工程学院江苏镇江212100

本文利用电化学阻抗谱和动电位极化曲线研究了去应力退火对激光粉末床熔融(LPBF)技术制备的TC4钛合金在1%NaCl溶液中的电化学行为,并采用Mott-Schottky测试、X射线光电子能谱仪对其表面钝化膜进行了分析。结果表明:LPBF TC4合金由针状... 详细信息

本文利用电化学阻抗谱和动电位极化曲线研究了去应力退火对激光粉末床熔融(LPBF)技术制备的TC4钛合金在1%NaCl溶液中的电化学行为,并采用Mott-Schottky测试、X射线光电子能谱仪对其表面钝化膜进行了分析。结果表明:LPBF TC4合金由针状α'/α马氏体组成,而800℃去应力退火后的组织为α+β双相结构;去应力退火导致LPBF TC4钛合金的耐蚀性降低,极化电阻降低,腐蚀电流密度和钝化电流密度增加。退火处理前后LPBF TC4钛合金的钝化膜均为n-型半导体,但去应力退火后合金的载流子密度增加,且钝化膜中TiO2的含量降低,导致合金的耐腐蚀性能降低。

关键词：腐蚀电化学钝化膜 TC4钛合金激光粉末床熔融

单增李斯特菌毒力因子溶血素关键氨基酸位点在感染过程中的作用研究

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微生物学报 2024年第4期64卷 1219-1232页

作者：冯昱超徐加利朱斌杰吴泽斌王雯静丁桉蔡孜萌陈绵绵孙静江铃丽宋厚辉夏菁程昌勇浙江农林大学动物科技学院·动物医学院、浙江省畜禽绿色生态健康养殖应用技术研究重点实验室、动物健康互联网检测技术浙江省工程研究中心、浙江省动物医学与健康管理国际科技合作基地、中澳动物健康大数据分析联合实验室浙江杭州311300 宁波卫生职业技术学院浙江宁波315100

【目的】探究单增李斯特菌溶血素O(listeriolysin O,LLO)中D3区域β8折叠片上第253位氨基酸(谷氨酰胺,Q)和第254位氨基酸(异亮氨酸,I)对单增李斯特菌(Listeria monocytogenes)感染生物学功能的影响。【方法】构建LLO_(Q253A)和LLO_(I25... 详细信息

【目的】探究单增李斯特菌溶血素O(listeriolysin O,LLO)中D3区域β8折叠片上第253位氨基酸(谷氨酰胺,Q)和第254位氨基酸(异亮氨酸,I)对单增李斯特菌(Listeria monocytogenes)感染生物学功能的影响。【方法】构建LLO_(Q253A)和LLO_(I254A)突变蛋白的原核表达菌株,以及利用同源重组方法构建hly_(Q253A)和hly_(I254A)突变株;通过表达纯化突变蛋白,测定溶血活性;比较LLO第253位Q和第254位I均突变成丙氨酸(A)后,对细菌体外生长能力、黏附侵袭、胞内迁移和增殖能力的影响。【结果】相应位点突变后,LLO蛋白均能够正常表达。在pH 6.5条件下,所有突变蛋白和突变株的溶血活性丧失。然而,在pH 5.5条件下,LLO_(I254A)和hly_(I254A)恢复了溶血活性。与野生株相比,突变株的体外生长、黏附能力和胞内增殖能力均无明显差异;突变株的侵袭能力和胞间迁移能力显著低于野生株。【结论】本研究证实第253位Q和第254位I均突变成A后,单增李斯特菌在pH 6.5条件下丧失溶血活性,并降低了感染宿主细胞的能力,但具体机制还有待进一步探索。本研究为深入探究LLO结构对单增李斯特菌生物学功能的影响奠定基础,对单增李斯特菌点突变株的构建具有一定参考意义。

关键词：单增李斯特菌溶血素O 感染点突变缺失株

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溃疡性结肠炎患者睡眠质量研究现状

天津护理 2024年第1期32卷 108-111页

作者：张楠王晓冉李玉伶马宏文李紫梦孙垚天津市人民医院天津300121

溃疡性结肠炎患者睡眠质量较差,影响患者疾病康复与生活质量。检索文献对溃疡性结肠炎患者睡眠质量的测量方法、现状、相关因素与干预措施等进行综述,为改善溃疡性结肠炎患者的睡眠质量提供依据。

关键词：溃疡性结肠炎睡眠质量生活质量